miRBase entry: hsa-mir-744

Stem-loop hsa-mir-744

Homo sapiens hsa-mir-744 precursor miRNA
Gene family
MIPF0000431; mir-744

Caution, this is an AI generated summary based on literature. This may have errors. ?

MIR744 is a non-conserved miRNA that has been discovered in one or a few species [PMC9879538]. It is involved in the post-transcriptional regulation of TGF-beta1, which plays a role in cellular processes such as proliferation, differentiation, migration, and survival [PMC3828615]. In breast cancer (BC), MIR744 has been found to be hypomethylated and up-regulated in 56% of cases [PMC3828615]. It is also activated by bortezomib-mediated activation of CEBPD [PMC8308509]. MIR744 is one of the miRNA regulators expressed in cumulus cells (CCs), along with MIR425, MIR146b, Let-7d, MIR202, and Let-7e [PMC4168028]. Interestingly, MIR202 has been identified as a potential regulator of the aging and angiogenesis-related gene HAS2 [PMC4168028]. NONO interacts with several miRNAs including MIR744 and protein-coding genes such as CELF2 and KRT8 [PMC9730017]. In addition to BC, MIR744 has also been associated with poor prognosis in nasopharyngeal carcinoma (NPC) [PMC6368411]. It has been shown to induce the expression of Cyclin B1 in mouse cell lines along with miR1186 and miR466d-3p [PMC4696257]. The 5'-flanking region of MAP2K4 (host gene of intronic MIR744) was cloned from THP-1 cells using PCR techniques [PMC5775404]. The mature sequence of MIR744 was synthesized to generate a construct that inhibits its expression for functional studies [PMC5362436].

Literature search
47 open access papers mention hsa-mir-744
(449 sentences)


159015 reads, 697 reads per million, 134 experiments

--     c     GC    C   -   U       gucuuacugaa      c 
  uuggg aaggU  GGGG UAG GGC AACAGCA           gguuuc  
  ||||| |||||  |||| ||| ||| |||||||           |||||| u
  ggcuc uUCCA  CUCC AUC CCG UUGUCgu           ccaaag  
cu     a     -A    A   A   -       ----acacgca      g 

Annotation confidence High
Do you think this miRNA is real?
This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was later confirmed by cloning [2].

Genome context
chr17: 12081899-12081996 [+]

Disease association
hsa-mir-744 is associated with one or more human diseases in the Human microRNA Disease Database
Disease Description Category PubMed ID

Database links

Mature hsa-miR-744-5p

Accession MIMAT0004945
Description Homo sapiens hsa-miR-744-5p mature miRNA
Evidence experimental
cloned [2]
Database links
Predicted targets

Mature hsa-miR-744-3p

Accession MIMAT0004946
Description Homo sapiens hsa-miR-744-3p mature miRNA
Evidence experimental
cloned [2]
Database links
Predicted targets


  1. PubMed ID: 17604727
    A mammalian microRNA expression atlas based on small RNA library sequencing
    "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
    "Cell (2007) 129:1401-1414

  2. PubMed ID: 16954537
    Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
    "Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E"
    "Genome Res (2006) 16:1289-1298