Accession
MI0002074
Description
Danio rerio
dre-mir-454a precursor miRNA mir-454
Gene
family?
family?
RF00746;
mir-454
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
Dre-mir-454a is a microRNA (miRNA) in zebrafish that has been evaluated for its stability as a reference gene in gene expression studies [PMC8613261]. According to the Normfinder software analysis, dre-mir-454a ranked as the third most stable miRNA with a stability value of 0.014, suggesting its potential utility as an endogenous control [PMC8613261]. However, when expression data from methimazole (MTZ) treatments were considered, dre-mir-454a's stability ranking dropped slightly to the seventh position with a value of 0.018 [PMC8613261]. Additionally, when comparing across three different zebrafish strains, dre-mir-454a's ranking was fourth in terms of stability with a value of 0.026 [PMC8613261]. The study investigated nine miRNAs including dre-mir-454a for their suitability as endogenous reference genes, indicating the importance of selecting stable reference genes for accurate normalization in gene expression analysis [PMC8613261].
Literature search
1 open access papers mention dre-mir-454a
(1 sentences)
(1 sentences)
Sequence
2725
reads,
167
reads per million, 11 experiments
uugcaggucguuuaagaauuaauccuaauucuugggacccuaucaguauugccucugcuguccacuguguucagagUAGUGCAAUAUUGCUAAUAGGGuuuuagguuuuagguuguaccuucag
.((.((((...............(((((..((((((((((((((((((((((..(((((.....(((....)))))))).)))))))))...)))))))))))))..)))))....)))).)).
.((.((((...............(((((..((((((((((((((((((((((..(((((.....(((....)))))))).)))))))))...)))))))))))))..)))))....)))).)).
Structure
u c cguuuaagaauuaau uu --- cu gucca u ug aggu ccuaa cuugggacccuau caguauugc cugcu cug g || |||| ||||| ||||||||||||| ||||||||| ||||| ||| ac ucca ggauu ggauuuuGGGAUA GUUAUAACG GAUga gac u g u -----------uguu uu AUC -U ----- u
Annotation confidence
Not enough data
Do you think this miRNA is real?
Genome context
chr10: 33376818-33376941 [-]
Clustered miRNAs
2 other miRNAs are < 10 kb from dre-mir-454a
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature dre-miR-454a
| Accession | MIMAT0001877 |
| Description | Danio rerio dre-miR-454a mature miRNA |
| Sequence | 77 - UAGUGCAAUAUUGCUAAUAGGG - 98 |
| Evidence |
experimental
cloned [1] |
| Database links |
|
| Predicted targets |
|
References
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