miRBase entry: cel-mir-259

Stem-loop cel-mir-259

Caenorhabditis elegans cel-mir-259 precursor miRNA
Gene family
MIPF0000299; mir-259

Literature search
2 open access papers mention cel-mir-259
(57 sentences)


2189 reads, 148 reads per million, 14 experiments

---uc        --a       A        -  U    U   A   ucuuu 
     agucauug   aacgagu AAUCUCAU CC AAUC GGU GCA     c
     ||||||||   ||||||| |||||||| || |||| ||| |||      
     ucagugau   uuguuCA UUAGGGUA GG UUAG CCA Cgu     g
uuuuu        aaa       G        C  U    -   C   uccaa 

Annotation confidence High
Do you think this miRNA is real?
miR-259 was predicted by computational analysis and conservation in C. elegans and C. briggsae, and the microRNA confirmed by PCR amplification, cloning and sequencing [1].

Genome context
chrV: 10539025-10539125 [-]

Database links

Mature cel-miR-259-5p

Accession MIMAT0000314
Description Caenorhabditis elegans cel-miR-259-5p mature miRNA
Evidence experimental
cloned [1-3], Illumina [4,6], CLIPseq [5]
Database links
Predicted targets

Mature cel-miR-259-3p

Accession MIMAT0020342
Description Caenorhabditis elegans cel-miR-259-3p mature miRNA
Evidence experimental
Illumina [6]
Database links


  1. PubMed ID: 12747828
    MicroRNAs and other tiny endogenous RNAs in C. elegans
    "Ambros V, Lee RC, Lavanway A, Williams PT, Jewell D"
    "Curr Biol (2003) 13:807-818

  2. PubMed ID: 17174894
    Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
    "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
    "Cell (2006) 127:1193-1207

  3. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  4. PubMed ID: 20062054
    Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans
    "Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW"
    "Nat Struct Mol Biol (2010) 17:173-179

  5. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322

  6. PubMed ID: 21307183
    Improved annotation of C. elegans microRNAs by deep sequencing reveals structures associated with processing by Drosha and Dicer
    "Warf MB, Johnson WE, Bass BL"
    "RNA (2011) 17:563-577