Accession
MI0000315
Description
Caenorhabditis elegans
cel-mir-239b precursor miRNA mir-239
Gene
family?
family?
RF00713;
mir-239
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
Cel-mir-239b is a microRNA from Caenorhabditis elegans used as a negative control in various studies due to its minimal sequence identity with miRNAs in human, mouse, and rat [PMC2699527]. It is employed to differentiate the specific effects of other miRNAs being investigated, as it does not target human genes [PMC7708977]. In experiments involving the FXN gene, cel-mir-239b did not cause a decrease in FXN mRNA and protein levels, unlike specific human miRNAs such as hsa-miR-224-5p [PMC7253519]. This control microRNA is used across various cell types and conditions to ensure that observed effects can be attributed to the experimental miRNA rather than nonspecific effects of transfection or experimental manipulation [PMC7708977][PMC9617134][PMC4260481][PMC4546478[PMC9617134][PMC4260481][PMC4546478]. Cel-mir-239b is also used in gene regulation studies as a negative control to validate the specificity of miRNA-mimic or inhibitor effects on target gene expression [PMC6027187]. Additionally, it serves as a control in vivo when assessing the biological impact of other miRNAs on physiological processes or disease models [PMC5460990]. Overall, cel-mir-239b functions as an essential tool for validating experimental results by providing a baseline against which the activity of other microRNAs can be measured [PMC3743786][PMC8019740][PMC3572043][PMC6502783][PMC4308918][PMC5460990][PMC8019740][PMC3572043][PMC6502783][PMC4308918][PMC5460990][ PMC3422229] [ PMC4104030] .
Literature search
4 open access papers mention cel-mir-239b
(45 sentences)
(45 sentences)
Sequence
16083
reads,
130
reads per million, 14 experiments
gcgacagaugcaauuUUUGUACUACACAAAAGUACUGgucauuuaaguugaggcucaGCACUUUUGUGGUGUGCAAAAAuggcaaguugcuuuuaucu
(((((...(((.((((((((((..(((((((((.((((((.((......))))).))).)))))))))..)))))))))).))).)))))........
(((((...(((.((((((((((..(((((((((.((((((.((......))))).))).)))))))))..)))))))))).))).)))))........
Structure
-------- aga a UA A - a ua
gcgac ugc auuUUUGUAC CACAAAAGU CUG guc uu a
||||| ||| |||||||||| ||||||||| ||| ||| ||
cguug acg uAAAAACGUG GUGUUUUCA Gac cgg ag g
ucuauuuu --a g UG C u - uu
Annotation confidence
High
Do you think this miRNA is real?
Comments
miR-239b was predicted by computational analysis and conservation in C. elegans and C. briggsae, and the microRNA confirmed by PCR amplification, cloning and sequencing [1]. A large scale cloning and sequencing study finds two dominant mature miRNA products: the second is 1 nt shorter at the 5' end [3].
Genome context
chrX: 11791302-11791399 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-239b
| Name | Accession | Chromosome | Start | End | Strand | Confidence |
|---|
Database links
Mature cel-miR-239b-5p
| Accession | MIMAT0000295 |
| Description | Caenorhabditis elegans cel-miR-239b-5p mature miRNA |
| Sequence | 16 - UUUGUACUACACAAAAGUACUG - 37 |
| Evidence |
experimental
cloned [3], Illumina [4], CLIPseq [5] |
| Database links |
|
| Predicted targets |
|
Mature cel-miR-239b-3p
| Accession | MIMAT0015115 |
| Description | Caenorhabditis elegans cel-miR-239b-3p mature miRNA |
| Sequence | 58 - GCACUUUUGUGGUGUGCAAAAA - 79 |
| Evidence |
experimental
CLIPseq [5] |
| Database links |
|
References
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