gcu uaggua c au - AUC ucuu
gcuggaaggug cc uca ggCUCAGUAGCCAG UGUAG CUg u
||||||||||| || ||| |||||||||||||| ||||| ||| c
cgaucuucuac gg agu cUGGGUCAUCGGUC ACAUC gac g
--u ------ u cu U GAc uaau
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This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences . Expression of the excised miR was validated in zebrafish, and the ends mapped by cloning. Subsequent cloning studies have also verified the expression of miR-222 in human ES cells. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .