Accession
MI0000077
Symbol
HGNC:
MIR21
Description
Homo sapiens
hsa-mir-21 precursor miRNA mir-21
Gene
family?
family?
RF00658;
mir-21
Summary
Caution, this is an AI generated summary based on literature. This may have errors. ?
To examine the signaling pathways involved in MIR21 mimic-induced wound healing responses, western blot analysis was performed on wounded tissues on day 5 [PMC7053209]. Serum MIR21, CEA, NSE, and CYFRA21-1 levels were, on average, significantly higher in patients with NSCLC than in control individuals (Table 1) [PMC4737022]. However, serum MIR21 and CYFRA21-1 levels of NSCLC patients at TNM stages I-II were significantly lower than those of patients at TNM stages III-IV (t = 2.575, −2.301, resp [PMC4737022]. Comparison of joint detection of serum CYFRA21-1 and MIR21 levels with single detection of either marker indicated that AUC of single detection for CYFRA21-1, single detection for MIR21, and joint detection of both markers were 0.843, 0.872, and 0.909, respectively (Figure 1(c), Table 6) [PMC4737022]. Therefore, this study comparatively analyzed the value of MIR21 compared to tumor markers CEA, NSE, and CYFRA21-1 for early diagnosis of NSCLC [PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022'>PMC4737022]. However, logistic multiple regression analysis indicated that early NSCLC (TNM stages I-II) was associated with serum CYFRA21-1 and MIR21 levels [PMC4737022]. Joint detection of MIR21 with serum CYFRA21-1 can further improve diagnostic efficiency for early NSCLC [PMC4737022]. The results indicated that occurrence of early NSCLC was significantly correlated with serum CYFRA21-1 (OR = 1.076; Wald χ 2 = 4.025) and MIR21 (OR = 2.473; Wald χ 2 = 9.153) levels (P < 0.05) (Table 5) [PMC4737022]. MIR21 had the highest diagnostic efficiency for early NSCLC (Figure 1(b), Table 4) [PMC4737022]. Therefore, MIR21 could serve as an important serum marker for auxiliary diagnosis of early NSCLC, while joint detection of serum MIR21 and CYFRA21-1 levels could improve diagnostic efficiency [PMC4737022]. This study indicates that, on average, serum MIR21 and CYFRA21-1 levels were lower in patients with NSCLC at TNM stages I-II than NSCLC at TNM stages III-IV [PMC4737022]. Serum MIR21, CEA, NSE, and CYFRA21-1 levels did not significantly differ with gender or age of NSCLC patients or with tumor pathology (Table 2) [PMC4737022]. AUC analysis showed that MIR21 had the highest diagnostic efficiency for early NSCLC [PMC4737022]. After removing the data for TNM stages III-IV, the AUC of using serum MIR21, CEA, NSE, and CYFRA21-1 levels to diagnose early NSCLC (TNM stages I-II) was 0.752, 0.806, 0.843, and 0.882, respectively [PMC4737022]. To validate the relationship between MIR21 expression and AUY922 sensitivity, we conducted a high-throughput compound screen in RKO cells that had been engineered to knock out the MIR21 locus (MIR21KO) and parental isogenic wild type (WT) cells.40 A number of HSP90 inhibitors produced a larger reduction in cell viability in MIR21KO cells in comparison with WT cells (Supplementary Figure 2A), with AUY922, 17-AAG, 17-DMAG, and ganetespib showing the highest activity [PMC5863695]. To validate the role of MIR21 in driving resistance to HSP90 inhibition, we infected MIR21KO DLD-1 cells with an inducible MIR21 or control (CTRL) viral vector (Supplementary Figure 2F) and monitored their response to AUY922 [PMC5863695]. A multiplex sandwich immunoassay showed a reduction in the level of HSP40 (
Literature search
1559 open access papers mention hsa-mir-21
(13078 sentences)
(13078 sentences)
Sequence
6832853
reads,
28570
reads per million, 159 experiments
ugucgggUAGCUUAUCAGACUGAUGUUGAcuguugaaucucauggCAACACCAGUCGAUGGGCUGUcugaca
((((((((((((((((.(((((.(((((.((((.((...))))))))))).)))))))))))))))))))))
((((((((((((((((.(((((.(((((.((((.((...))))))))))).)))))))))))))))))))))
Structure
A A A u a
ugucgggUAGCUUAUC GACUG UGUUG cugu ga
|||||||||||||||| ||||| ||||| |||| || u
acagucUGUCGGGUAG CUGAC ACAAC ggua cu
- C - - c
Annotation confidence
High
Do you think this miRNA is real?
Comments
Mourelatos et al. named this sequence miR-21 precursor-17 and also reported the exact reverse complement of this predicted stem-loop sequence and erroneously assigned the name miR-104 [2].
Genome context
chr17: 59841266-59841337 [+]
Disease association
hsa-mir-21 is associated with one or more human diseases in the Human microRNA Disease Database
| Disease | Description | Category | PubMed ID |
|---|
Biological pathways
hsa-mir-21 is involved in one or more biological pathways:
(Source: Reactome)
(Source: Reactome)
Biological reactions
hsa-mir-21 is involved in one or more regulation/signalling events:
(Source: Reactome)
(Source: Reactome)
Database links
Mature hsa-miR-21-5p
| Accession | MIMAT0000076 |
| Description | Homo sapiens hsa-miR-21-5p mature miRNA |
| Sequence | 8 - UAGCUUAUCAGACUGAUGUUGA - 29 |
| Evidence |
experimental
cloned [1-3,6-9], Northern [1,5], Illumina [10] |
| Database links |
|
| Predicted targets |
|
Mature hsa-miR-21-3p
| Accession | MIMAT0004494 |
| Description | Homo sapiens hsa-miR-21-3p mature miRNA |
| Sequence | 46 - CAACACCAGUCGAUGGGCUGU - 66 |
| Evidence |
experimental
cloned [8-9] |
| Database links |
|
| Predicted targets |
|
References
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