8 publications mentioning mdm-MIR168a

Open access articles that are associated with the species Malus domestica and mention the gene name MIR168a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Several miRNAs were differentially expressed across the stem tissues: miR160 showed higher expression in the phloem than xylem and was barely detectable in periderm; miR394 showed higher expression in the xylem than phloem and was barely detectable in periderm; miR168 was more abundant in periderm than phloem and xylem; miR171 was absent from phloem but was detected both in periderm and xylem.

Conservation status miRNA family Arabidopsis Oryza(rice) Populus(poplar) Predicted target gene(s) miR156 √ √ √Squamosa promoter -binding proteins[57] miR159/319 √ √ √GAMYB transcription factors[57] miR160 √ √ √Auxin response factors (ARF) [57] miR162 √ √ √DICER-LIKE 1 (DCL1) [57] miR164 √ √ √NAC domain transcription factors[57] miR156/166 √ √ √HD-ZIP transcription factors[57] miR167 √ √ √Auxin response factors (ARF) [57] miR168 √ √ √ARGONAUTE 1 (AGO1) [57] miR169 √ √ √HAP2-like transcription factors[57] miR171 √ √ √Scarecrow-like transcription factors[57] miR172 √ √ √APETALA 2 transcription factors[58] miR390 √ √ √TAS3[59] miR393 √ √ √F-box transcription factors (TIR1) [60] miR394 √ √ √F-box transcription factors[60] miR396 √ √ √GRF, rhodenase[60] miR397 √ √ √laccase[60] miR398 √ √ √Copper superoxid dismutase, CytC oxidase[60] miR403 √ √ √ARGONAUTE 2 (AGO2)[20] miR408 √ √Peptide chain release factor, laccase[20] miR475 √PPR proteins[8] miR476 √PPR proteins[8] Figure 1 Differential expression of miRNAs in apple tissues.

Conservation status miRNA family Arabidopsis Oryza(rice) Populus(poplar) Predicted target gene(s) miR156 √ √ √Squamosa promoter -binding proteins[57] miR159/319 √ √ √GAMYB transcription factors[57] miR160 √ √ √Auxin response factors (ARF) [57] miR162 √ √ √DICER-LIKE 1 (DCL1) [57] miR164 √ √ √NAC domain transcription factors[57] miR156/166 √ √ √HD-ZIP transcription factors[57] miR167 √ √ √Auxin response factors (ARF) [57] miR168 √ √ √ARGONAUTE 1 (AGO1) [57] miR169 √ √ √HAP2-like transcription factors[57] miR171 √ √ √Scarecrow-like transcription factors[57] miR172 √ √ √APETALA 2 transcription factors[58] miR390 √ √ √TAS3[59] miR393 √ √ √F-box transcription factors (TIR1) [60] miR394 √ √ √F-box transcription factors[60] miR396 √ √ √GRF, rhodenase[60] miR397 √ √ √laccase[60] miR398 √ √ √Copper superoxid dismutase, CytC oxidase[60] miR403 √ √ √ARGONAUTE 2 (AGO2)[20] miR408 √ √Peptide chain release factor, laccase[20] miR475 √PPR proteins[8] miR476 √PPR proteins[8] Figure 1 Differential expression of miRNAs in apple tissues.

C, Stem-loop RT-PCR analyses using 10 ng total RNA of miR160, miR162, miR164, miR168, miR169, miR171, miR390, miR393, miR394, miR396, miR397, miR398, miR403, miR408, miR475, and miR476 expression.

Using this approach miR156, miR159, miR160, miR162, miR167, miR169, miR396 and miR398 were clearly detectable; miR172, miR390 and miR393 produced a weak amplification signal; miR166 and miR397 amplification did not produce the expected product, but resulted in a smear not detected in the minus-RT control; miR164, miR168, miR171, miR394, miR403, miR408 and the miRNAs specific to poplar (miR475 and miR476) were not detected (Figure 4).

Some exceptions suggest differences in phloem-mobility of specific miRNAs among species or possibly between herbaceous and woody plants; in particular, miR168 and miR408 accumulated in the phloem sap of pumpkin and Brassica napus, but could not be amplified from the apple phloem sap.

miR168 and miR408 were not detected in the apple SE sample but were enriched in the pumpkin phloem sap sample (Table 2).

2

For example, the expression of gma-miR167 is induced by far-red light (Jones-Rhoades and Bartel, 2004; Zhang et al., 2014a), the expression levels of ath-miR408 is induced by light (Li et al., 2014; Zhang et al., 2014a), and the expression levels of ath-miR396 (Zhou et al., 2007), ptc-miR168a-b, ptc-miR169, and ptc-miR398 (Jia et al., 2009) are induced by UV-light.

3

This number was soon increased to include miR168 (inhibiting ARGONAUTE1), miR172 (inhibiting APETALA2) (Itaya et al., 2008), and miR156 (targeting CNR) (Zhang et al., 2011).

4

It was found that mdm-MIR168b-p5, ppe-MIR168-p5, mdm-miR159c, and ppe-miR530_L-2R+2 could all target AGO1 in apple.

However, AGO1 in Arabidopsis was regulated by miR168 (Vaucheret et al., 2004).

Among them, two are from the miR168 family, and another two are from miR159 and miR530 families (Figure 7).

5

Although lower expression (between 1,500 and 4,000 RPM) was observed for the miR162, miR164, miR168, miR172 and miR399 families, their overall expression level was 3 to 20 times greater than any of the remaining 12 conserved miRNA families (Figure 1a; Table S3 in Additional file 1).

6

The juvenile to adult phase transition related microRNAs, mdm-miR156 and mdm-miR172, and the flowering related, mdm-miR535, mdm-miR168, and mdm-miR167, also showed high expression levels in apple shoot tips.

7

Transcriptional regulation of Arabidopsis MIR168a and argonaute1 homeostasis in abscisic acid and abiotic stress responses.

8

However, while the evidence in support of bioavailability of milk miRNAs is unambiguous, a recent report that mammals can also absorb plant miRNAs (e. g. miR-168a) from rice [25] was met with widespread skepticism [26– 29].