miRNA Search Results

sort by 2 publications mentioning rno-mir-3562 Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-3562. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.
1	[+] score: 28 Other miRNAs from this paper: rno-mir-324, rno-mir-328a, rno-mir-24-1, rno-mir-24-2, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-204, rno-mir-667, rno-mir-3568, rno-mir-3584-1, rno-mir-702, rno-mir-3584-2 By both the computational methods (Miranda and Targetscan) we demonstrated that in the enriched set, miR-328a-star_st was found to control maximum number of targets (381) followed by miR-3562 controlling 341 putative targets. [score:7] Our microarray analysis and target prediction using computational methods for the 33 miRNAs revealed that the representative set of miRs: miR-667, miR-702, miR-3562, miR-3568 and miR-3584 either controlled genes of interest in the formation of MV, or were predicted to target many genes, with significantly higher expression in MV compared to VSMC from microarray data analysis (Table 1 & Fig 3). [score:6] To validate the miRNA identified by the arrays that regulate multiple genes, we performed Real time PCR on VSMc and MV to determine the expression of miR-667, miR-702, miR-3562, mir-3568 and miR-3584 and normalized by U6. [score:4] 0131589.g007 Fig 7 To validate the miRNA identified by the arrays that regulate multiple genes, we performed Real time PCR on VSMc and MV to determine the expression of miR-667, miR-702, miR-3562, mir-3568 and miR-3584 and normalized by U6. [score:4] The results demonstrated that miR-667, miR-702, miR-3562, miR-3568 and miR-3584 are all expressed in VMSC from CKD rats and are highly concentrated in MV, confirming directional changes from microarray analysis (Fig 7). [score:4] Target-specific PCR primers (miR-667, miR-702, miR-3562, miR-3568 and miR-3584) were obtained from Applied Biosystems. [score:2] We also confirmed mir-667 and miR-3562, as these miRNA controlled several genes associated with vesicle -mediated transport and thus may play a role in the formation of MV. [score:1] [1 to 20 of 7 sentences]
2	[+] score: 4 Other miRNAs from this paper: rno-mir-327, rno-mir-19a, rno-mir-23a, rno-mir-30b, rno-mir-32, rno-mir-130a, rno-mir-134, rno-mir-183, rno-mir-210, rno-mir-207, rno-mir-20b, rno-mir-182, rno-mir-423, rno-mir-465, rno-mir-466d, rno-mir-3588, rno-mir-1249, rno-mir-328b, rno-mir-1306, rno-mir-155 We found that 14 of the DEmiRNAs identified in our screen can regulate 2927 common putative target genes (Figure 5A), while 9 miRNAs, including miR-1249, miR-1306-3p, miR-32-3p, miR-327, miR-328b-3p, miR-3562, miR-3588, miR-423-5p, and miR-466d, had no common predicted genes. [score:4] [1 to 20 of 1 sentences]

By both the computational methods (Miranda and Targetscan) we demonstrated that in the enriched set, miR-328a-star_st was found to control maximum number of targets (381) followed by miR-3562 controlling 341 putative targets. [score:7]

Our microarray analysis and target prediction using computational methods for the 33 miRNAs revealed that the representative set of miRs: miR-667, miR-702, miR-3562, miR-3568 and miR-3584 either controlled genes of interest in the formation of MV, or were predicted to target many genes, with significantly higher expression in MV compared to VSMC from microarray data analysis (Table 1 & Fig 3). [score:6]

To validate the miRNA identified by the arrays that regulate multiple genes, we performed Real time PCR on VSMc and MV to determine the expression of miR-667, miR-702, miR-3562, mir-3568 and miR-3584 and normalized by U6. [score:4]

0131589.g007 Fig 7 To validate the miRNA identified by the arrays that regulate multiple genes, we performed Real time PCR on VSMc and MV to determine the expression of miR-667, miR-702, miR-3562, mir-3568 and miR-3584 and normalized by U6. [score:4]

The results demonstrated that miR-667, miR-702, miR-3562, miR-3568 and miR-3584 are all expressed in VMSC from CKD rats and are highly concentrated in MV, confirming directional changes from microarray analysis (Fig 7). [score:4]

Target-specific PCR primers (miR-667, miR-702, miR-3562, miR-3568 and miR-3584) were obtained from Applied Biosystems. [score:2]

We also confirmed mir-667 and miR-3562, as these miRNA controlled several genes associated with vesicle -mediated transport and thus may play a role in the formation of MV. [score:1]

[1 to 20 of 7 sentences]

miRBase

2 publications mentioning rno-mir-3562