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30 publications mentioning ssc-mir-206

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-206. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 100
Other miRNAs from this paper: ssc-mir-133a-1, ssc-mir-1, ssc-mir-133b, ssc-mir-133a-2
In postnatal muscle, miRNA-206 was down-regulated from D0 to D40 and was up-regulated from D40 to D100 (Figure  4D). [score:7]
SFRP1 miRNA-206 miRNA-1 Skeletal muscle Development Pig The Wnt signaling pathway plays an essential role during embryonic and postnatal muscle development [1, 2] because it regulates the expression of myogenic regulatory factors, which are essential for myogenic lineage progression and the formation of functional multinucleated myotubes [3, 4]. [score:7]
miRNA-206, meanwhile, was abundantly expressed in skeletal muscle and heart, and up-regulated from pre- to postnatal-stage skeletal muscle. [score:6]
To further explore the biological functions and regulatory mechanisms of SFRP1 gene and miRNA-1/206 in porcine muscle development, we analyzed the temporal and spatial expression patterns of miRNA-206 and SFRP1 in prenatal and postnatal skeletal muscle at 20 developmental stages. [score:6]
The expression of miRNA-206 was weak in skeletal muscle in the early embryonic stages, but then remained stable with high levels of expression in the remaining prenatal stages, although some fluctuations occurred. [score:5]
miRNA-1 and miRNA-133 are expressed in both cardiac and skeletal muscles [25] and miRNA-206 is only expressed in skeletal muscle [26]. [score:5]
We performed a temporal-spatial expression analysis of SFRP1 mRNA and miRNA-206 in Tongcheng pigs (a Chinese indigenous breed) by quantitative real-time polymerase chain reaction, and conducted the co -expression analyses of SFRP1 and miRNA-1/206. [score:5]
According to their study and this study, miRNA-1 and miRNA-206 were abundantly expressed in skeletal muscle and weakly expressed in other tissues. [score:5]
However, miRNA-206 was abundantly expressed in the longissimus dorsi muscle and heart, and was weakly expressed in the other tissues (Figure  4B). [score:5]
Developmental expression of porcine SFRP1 mRNA and miRNA-206. [score:4]
miRNA-1 and miRNA-206 were abundant in the postnatal stages and were at low levels in the prenatal stages of muscle development, while SFRP1 exhibited an opposite expression patterns. [score:4]
The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. [score:4]
Figure 4 Relative expression of SFRP1 and miRNA-206 in the different tissues and in skeletal muscle during development of Tongcheng pigs. [score:4]
The co -expression analysis revealed that the SFPR1 mRNA was significantly negatively correlated with miRNA-1 (Pearson’s R SFRP1/miRNA-1 = −0.928, p [value] = 0.003) (Figure  5A) and miRNA-206 (Pearson’s R SFRP1/miRNA-1 = −0.922, p [value] = 0.003) (Figure  5B) at the mRNA level in different tissues adult tissues. [score:3]
Our recent study documented that miRNA-1 and miRNA-206 were abundantly and specifically expressed in porcine skeletal muscle [30]. [score:3]
The expression level of miRNA-206 reached a peak at E100. [score:3]
The expression of SFRP1 mRNA and miRNA-206 was detected by real-time quantitative polymerase chain reaction (qPCR). [score:3]
Figure 8 Validating SFRP1 as a positive target for miRNA-1 and miRNA-206. [score:3]
We also explored the spatial-temporal profile of SFRP1 mRNA and miRNA-206 in adult tissues and in skeletal muscle tissues during development in Tongcheng pigs. [score:2]
These results demonstrated that miR-206 plays a key role in skeletal muscle development in pigs. [score:2]
Figure 9 The miRNA-1 and miRNA-206 regulate SFRP1 at the protein level. [score:2]
miRNA-1 and miRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing the paired box 7 (Pax7) gene [27]. [score:2]
Subsequently, miRNA-1and miRNA-206 mimics and a normal control (NC) were co -transfected into PIECs, and luciferase activity was detected. [score:1]
The porcine SFRP1-CDS-3′-UTR-psiCHECK-2 vector was constructed, and it was co -transfected with miRNA-1 and miRNA-206 mimics in PIECs. [score:1]
Figure 7 Predicted miRNA-1 and miRNA-206 binding sites (highlighted in red) in the 3′-UTR of SFRP1 showing species conservation. [score:1]
The sequence of porcine miRNA-206 was obtained from the miRBase database (Accession ID:MI0013084) (http://www. [score:1]
The expression of SFRP1 and miRNA-206 was measured in seven tissues from adult Tongcheng pigs. [score:1]
The luciferase activity of the psiCHECK-2- SFRP1–3′-UTR (mut) was not significantly decreased by both the miRNA-1 and miRNA-206 mimics (12.39% of control for the miRNA-1 mimic -transfected group and 16.76% of control for the miRNA-206 mimic -transfected group) (p >0.05) (Figure  8). [score:1]
Cotransfection of porcine pre-miRNA-1 (A) and pre-miRNA-206 (B) or control and porcine SFRP1 UTR-derived psiCHECK-2 construct or mutant in PIEC cells. [score:1]
SFRP1 mRNA was significantly negatively correlated with miRNA-1/206 [Pearson’s R SFRP1/miRNA-1 = −0.445, p [value] = 0.032 (Figure  5C); Pearson’s R SFRP1/miRNA-206 = −0.480, p [value] = 0.049(Figure  5D)]. [score:1]
Distribution of porcine SFRP1 mRNA and miRNA-206 in tissuesThe expression of SFRP1 and miRNA-206 was measured in seven tissues from adult Tongcheng pigs. [score:1]
Distribution of porcine SFRP1 mRNA and miRNA-206 in tissues. [score:1]
[1 to 20 of 32 sentences]
2
[+] score: 82
According to the and the prediction results of TargetScan and PicTar, RAP2C and SMARCD1 were potential target genes of miR-133b, and MAP1A, SFRS3 and CNN3 were potential target genes of miR-206 (Table 4). [score:7]
Using the Targetscan prediction program, for example, a total of 478, 435 and 478 mRNA targets were obtained for miR-1, miR-133 and miR-206, respectively. [score:5]
A previous study suggested that miR-1, miR-133b and miR-206 are associated with skeletal muscle development; we also found that these miRNAs were differentially expressed and critical to prenatal skeletal muscle development. [score:5]
miR-206 was up-regulated in both Landrace and Tongcheng pigs and peaked at 65 dpc in Wuzhishan pigs. [score:4]
The SFRP2 gene, which plays an active role in embryogenesis, especially in muscle development 62, was found to be a potential target of miR-1 and miR-206 in myogenesis in Tongcheng pigs. [score:4]
The expression of C9orf19 was potentially regulated by miR-1, miR-133b, miR-206 and let-7a. [score:4]
There were 22, 21, 10, 9 and 9 target mRNAs for miR-765, miR-302b, miR-1, let-7a and miR-206, respectively, suggesting that these miRNAs significantly contribute to prenatal skeletal muscle development in Tongcheng pigs. [score:4]
We also found that CCNJ had potentially common target loci: let-7a, let-7b, let-7d, miR-198, miR-206, miR-207, miR-325, miR-370 and miR-697. [score:3]
Additionally, we validated interaction potentiality above prediction results based on five potential mRNA targets selected (RAP2C and SMARCD1 for miR-133b, CNN3, MAP1A and SFRS3 for miR-206) by experimental method. [score:3]
Three differentially expressed miRNAs (miR-133b, miR-206 and miR-302b) were selected for data verification of the miRNA array. [score:3]
To validate the target CNN3 of miRNA miR-206, western blotting was used to analyze interaction of miRNA-mRNA at the protein level. [score:3]
In this study, we identified only 10, 27 and 34 mRNA potential targets for miR-1, miR-133b and miR-206, respectively. [score:3]
In addition, miR-206, miR-608, miR-325 and miR-762 were identified as regulators of glycolysis during prenatal skeletal muscle development. [score:3]
CKAP2, SFRS3, TPM3, YWHAB and YWHAQ were identified as targets of miR-206. [score:3]
Most miRNAs were differentially expressed in one pig breed, but we found that nine miRNAs (miR-133b, miR-206, miR-202, miR-302b, miR-320, miR-500, miR-625, miR-665 and miR-700) were common to all three breeds. [score:3]
Moreover, western blot suggested that miR-206 repressed the expression of CNN3 at the protein level (Fig. 7F). [score:3]
However, there were varied expression patterns for the muscle-specific miRNAs miR-133b and miR-206 in each breed. [score:3]
Eight miRNAs (miR-765, miR-302b, miR-1, let-7a, miR-206, miR-500, miR-546 and miR-705) targeted 86.1% of the mRNAs in Tongcheng pigs. [score:3]
miR-133b potentially regulated 4 mRNAs (C9orf19, NAV1, NNAT and SQLE), while miR-206 potentially regulated C9orf19, CKAP2, CNN3, FKBP1B, HMGCS1, MAP1A, NCALD, SFRP2 and VCAN. [score:3]
Of these miRNAs, nine (miR-133b, miR-206, miR-202, miR-302b, miR-320, miR-500, miR-625, miR-665 and miR-705) were differentially expressed in all three breeds. [score:3]
The miRNA-GO network analysis suggested that cell adhesion was regulated by six miRNAs (miR-1, miR-206, miR-302b, miR-625, miR-765 and miR-669c). [score:2]
Twenty-one mRNAs (ACTN1, AGPAT1, AMT, ANK1, C20orf186, CALM1, CCNJ, CXCL12, DHRS3, FOXO1A, IRS1, JTB, LDB3, LITAF, PGK1, RPIA, SARS, SLAMF8, TEAD1, TTR and UBE2D2) were potentially regulated by miR-206 in Wuzhishan pigs. [score:2]
FKBP1B and HMGCS1, which encode rate-limiting enzymes of the cholesterol synthesis pathway 61, were potentially regulated by let-7a, miR-1 and miR-206. [score:2]
and Tongcheng pigs, miR-206 peaked at 90 dpc, but it peaked at 65 dpc in Wuzhishan pigs. [score:1]
With the exception of miR-133b and miR-206, there have been no reports on the functions of these miRNAs in myogenesis. [score:1]
We selected three miRNAs (miR-133b, miR-206 and miR-302b) and twelve mRNAs (IGF2, TPM3, COL15A1, CSRP3, LAMA2, LAMA3, LAMB2, UCHL1, FNDC1, FEZ2, FGL2 and IGFBP7) to validate our miRNA and mRNA microarray data by qPCR. [score:1]
Porcine miR-206 and miR-133b sequences were synthesized by GenePharma Company in Shanghai. [score:1]
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3
[+] score: 65
A mutation in the myostatin gene of heavily muscled Belgian Texel sheep creates a target site for miR-1 and miR-206 containing RISC complexes in the 3' untranslated portion of the transcript, resulting in decreased translation of the myostatin protein and consequent increase in muscle mass. [score:8]
Of these, 7 targets were identified for the highest expressed miR during fetal development to the adulthood, miR-206, including genes that have been implicated in multiple myogenic processes (see Additional file 3) [50, 53- 56]. [score:6]
Initially, miR targets were predicted for a sub-set of the miR up-regulated in this experiment (miR-206, miR-338, miR-368, miR-376a, and miR-381). [score:6]
Expression of the muscle regulatory factor, myogenic factor 5, has been reported to regulate miR-1 and miR-206 transcription level in a chicken cell culture mo del [18]. [score:5]
These predicted targets for miR-206 include dystophia myotonica protein kinase, which has been implicated in myotonic dystrophy [55] and the transcription factor paired box gene 3 that regulates myogenic cell fate through the myogenic transcription factor MyoD [54]. [score:4]
In addition, over -expression of fibroblast growth factor-4 has been reported to decrease miR-206 abundance, resulting in developmental changes in the somite of developing chicken embryos [19]. [score:4]
Based on abundance level, these data suggest that miR-206 and miR-1 may have a greater role in fetal muscle development than miR-133, or their targets are higher in abundance. [score:4]
Data for the satellite cells and adult muscle is presented as the average of multiple transcriptome libraries presented in Additional file 1. In comparison to the high abundance of miR-206 in muscle tissue, porcine miR-1 had relatively moderate abundance that increased throughout development, similar to the pattern observed for this miR in mouse muscle development [16]. [score:3]
These data suggest that miR-1 and miR-206 play different roles in muscle development, with miR-1 affecting regulation of genes that require inactivation in later fetal stages and miR-206 having a more constant role in repressing genes immediately after differentiation. [score:3]
Data for the satellite cells and adult muscle is presented as the average of multiple transcriptome libraries presented in Additional file 1. In comparison to the high abundance of miR-206 in muscle tissue, porcine miR-1 had relatively moderate abundance that increased throughout development, similar to the pattern observed for this miR in mouse muscle development [16]. [score:3]
The data herein confirm these results in satellite cells and also demonstrate that miR-206 is present at a high level through most or all of fetal development, and continues through maturation of the adult pig. [score:2]
Overall, miR were lowly abundant throughout fetal development with the exception of let-7 and muscle specific miR-1 and miR-206. [score:2]
In contrast to miR-1 and miR-206, miR-133 was detected only at low levels in fetal development yet increased in the neonate and adult (Figure 3). [score:2]
MiR-206 sequences of this type were clustered into a single miR sequence identified as the predominant sequence. [score:1]
In addition to identification of mismatches in the miR-206 sequence, length of sequence at the 5' and 3' ends also differed (Table 1, see Additional file 2). [score:1]
The constant high-level presence at all stages following early differentiation suggests that the role of miR-206 is to repress functions associated with muscle precursor cells. [score:1]
miR-206 sequence variation. [score:1]
Sequence comparison to known miR identified the muscle-specific miR-206 as the highest abundant miR across all muscle samples, which represented greater than 60% of all miR present (Figure 3). [score:1]
Click here for file miR-206 sequence variation. [score:1]
Three of the five were observed more frequently without mismatches: miR-206, miR-24a, and miR-168a (a cross-contamination from the parallel control oligo processing, see). [score:1]
This contrasts sharply with the abundance of miR-206 in proliferating satellite cells at 1.8 per thousand (or 0.18%). [score:1]
In mouse C [2]C [12 ]cells, miR-206 is also lowly abundant in proliferating cells and has been reported to be induced during differentiation [38], suggesting that its presence is associated with the switch from precursor to mature muscle cell. [score:1]
Three muscle-specific miRNA (miR-1, miR-133, and miR-206) were identified to increase in abundance during muscle cell differentiation [10, 16, 17]. [score:1]
MiR-133 increases proliferation of C [2]C [12 ]myoblasts, whereas miR-206 and miR-1 promote differentiation [16]. [score:1]
Five observed tags, miR-168a, miR-206, miR-24a, miR-368, and miR-381, represented possible exceptions to the pattern of exact sequence conservation across positions 4–17 of the reference miR. [score:1]
The data provided represent the variation of miR-206 sequences identified in the current study. [score:1]
[1 to 20 of 26 sentences]
4
[+] score: 50
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-20a, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
Paula et al. (2017) showed that a short period of food restriction significantly increased the expression of miR-1, miR-206, miR-199, and miR-23a in fast muscle and significantly decreased the expression of miR-1 and miR-206 in slow muscle, while their targets (IGF-1 for miR-1, miR-206, and miR-199; mTOR for miR-199; and MFbx and PGC1a for miR-23a) exhibited negatively correlated expression profiles. [score:9]
Some muscle miRNAs have well-defined target genes, as miR-206 regulates IGF-1; miR-1, miR-122, and miR-462 control IGF-2a; the let-7 family regulates MSTN; miR-103 and miR-107 modulate GHR and FSHR; and miR-138 and miR-211 control LHR. [score:5]
miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression. [score:4]
In tilapia, Yan et al. (2012a) and Nachtigall et al. (2015) showed that miR-1, miR-133a, and miR-206 have similar expression patterns in adult males and females and may assist each other to accurately control the development of skeletal muscles, although they perform distinct biological functions. [score:4]
In the skeletal muscle, the main edible part of the fish, miR-1, miR-133a, and miR-206 have conserved expression patterns in all farmed fish species, which makes them interesting molecules for modulating muscle development and growth. [score:4]
Therefore, miR-206 could affect tilapia growth by modulating IGF-1 gene expression levels (Yan et al., 2014). [score:3]
In addition, miR-206 loss of function in vivo was shown to significantly improve tilapia growth performance by targeting insulin-like growth factor-1 (IGF-1) (Yan et al., 2013b). [score:3]
They recorded changes in the expression levels of eight miRNAs (miR-1a, miR-181a, miR-133a, miR-214, miR-133b, miR-206, miR-146, and miR-26a) shown to be involved in a strong resumption of myogenesis (Zhu et al., 2014). [score:3]
Duran et al. (2015) analyzed the impact of the miRNA-target interactions of miR-1/ hdac4, miR-133-a/b/ srf, miR-206/ pax7, and miR-499/ sox6 in fast- and slow-twitch skeletal muscles during growth. [score:3]
Differential expression of microRNA-206 in skeletal muscle of female Piedmontese and Friesian cattle. [score:3]
The expression pattern of 12 miRNAs, including mir-1, mir-133 and mir-206, was validated by real time PCR. [score:3]
MiR-133a promotes, in part, myocyte proliferation by repressing serum response factor (SRF) (Chen et al., 2006), whereas miR-206 plays an important role in regulating the differentiation of C2C12 myoblasts in vitro (Kim et al., 2006). [score:2]
Muscle-specific microRNA miR-206 promotes muscle differentiation. [score:1]
For instance, the role ofmiR-1, miR-206, and miR-133 during myoblast proliferation and differentiation is recognized to interfere in the hypertrophic growth of skeletal muscle. [score:1]
MiR-1, miR-133a, miR-133b, miR-206, and miR-499 have been shown to be involved in the control of genes related to myoblast proliferation and differentiation. [score:1]
They found that miR-1 and miR-206 may promote myoblast differentiation in fast- and slow-twitch muscles in adult individuals, while miR-133a/b acts earlier, promoting myoblast proliferation in juveniles. [score:1]
[1 to 20 of 16 sentences]
5
[+] score: 37
0057156.g005 Figure 5 Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
Notably, miR-133b and miR-206 in one cluster shared the same expression pattern and the same target, indicating a novel regulatory mechanism of miRNAs. [score:6]
Interestingly, miR-133b, miR-133a-3p and miR-206 were predicted to target FSHβ directly. [score:4]
Two miRNAs (ssc-miR-133b, ssc-miR-206) formed a miRNA cluster and located on the same chromosome with an intergenic region of 3766bp, which suggests the miRNA cluster tends to be co-expressed. [score:3]
More interestingly, miR-133b, miR-133a-3p and miR-206 were firstly identified as muscle-specific miRNAs with a role in promoting the proliferation of myoblasts and inhibiting their differentiation [41]. [score:3]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
In the present study, miR-133b and miR-206 located closely on chromosome 7, and separated by only 3766 bp fragment. [score:1]
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6
[+] score: 37
MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. [score:5]
miR-133 expression level of MS pigs was always higher than LW (P < 0.05), while miR-1 and miR-206 has not shown obvious expression patterns between these two breeds (Fig 8). [score:5]
MiR-206 can be upregulated by MyoD and MEF2, and can promote the differentiation of myoblast by targeting multiple genes, such as Id1-3, Pax 7, BDNF, Notch-3, Hmyb3 and Cx43 [41, 42]. [score:5]
However the expression patterns of miR-1 and miR-206 from 35 to 90 dpc are similar between LW and MS, and significantly affected by developmental stages (P [t] < 0.01). [score:4]
The expression levels of miR-133, miR-1 and miR-206 in Large White (LW) and Meishan (MS) pigs during skeletal muscle development at 35 to 90 dpc (E35, E55 and E90). [score:4]
Finally, the expression patterns of muscle specific miRNA (i. e., miR-1, miR-133 and miR-206) were detected in LW and MS. [score:3]
Interestingly, Among them, miR-133, miR-1, miR-206 and miR-148a were highly abundant in MS pigs, while let-7 family, miR-214 and miR-181 were highly expressed in LW. [score:3]
Some myogenesis related miRNAs (miR-133, miR-1, miR-206 and miR-148a) are highly abundant in MS pigs, while other miRNAs (let-7 family, miR-214, miR-181) highly expressed in LW. [score:3]
The expression levels of muscle specific miRNAs, including miR-133, miR-1 and miR-206, in longissimus dorsi of LW and MS on pregnancy days 35, 55 and 90 were analyzed. [score:3]
MiR-206 pushes the equilibrium toward differentiation by down -regulating Id1-3 and MyoR [12]. [score:1]
The difference of miRNAs (miR-133, miR-1 and miR-206) among six samples (the muscle of LW and MS fetus at each time point 35d, 55d and 90d) was analyzed through two-way ANOVA method, in which breeds and time points were factors. [score:1]
[1 to 20 of 11 sentences]
7
[+] score: 32
Chromatin immunoprecipitation showed that the myogenic regulatory factors MyoD and myogenin upregulated the expression of miR-1-1, miR-1-2, miR-133a-1, miR-133a-2 and miR-206 in myotubes [3]. [score:7]
Ssc-miR-206 was expressed only in skeletal muscles, while ssc-miR-1 was expressed only in the skeletal muscles and heart. [score:5]
MiR-1 and miR-133 are expressed both in cardiac and skeletal muscles [22], whereas miR-206 is expressed only in skeletal muscles [23], [24]. [score:5]
Although an increasing body of evidence shows that miRNAs play important roles in the regulation of skeletal muscle development, precise regulatory mechanisms of the biological functions of most miRNAs remain unclear, and most research is restricted to myomiRs, such as miR-1, miR-133 and miR-206. [score:4]
Nielsen et al. [21] showed that ssc-miR-1 and ssc-miR-206 are expressed at extremely high levels in the longissimus muscles of Danish Landrace/Yorkshire crossbred pigs of age 1.5–2 years; however, the abundance of other miRNAs was relatively low. [score:3]
MiR-1, miR-206 and miR-133 are classified as myomiRs, as they play important roles in the regulation of muscle development and differentiation. [score:3]
Previous experiments showed that miR-1 and miR-206 promoted the differentiation of myoblasts, while miR-133 promoted proliferation [1], [2]. [score:1]
Ssc-miR-206, ssc-miR-1 and ssc-miR-378 had the most reads in our Solexa results. [score:1]
Although ssc-miR-133, another type of myomiR, could also be detected in our Solexa results, the reads were moderate and were not as abundant as that of ssc-miR-1 and ssc-miR-206. [score:1]
In our results, the abundance of ssc-miR-1 and ssc-miR-206 was extremely high, which was consistent with their results. [score:1]
The qPCR results of ssc-miR-1 and ssc-miR-206 were consistent with previous reports. [score:1]
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8
[+] score: 24
of Host miRNAs In silico target prediction were performed for those most abundant and DE porcine miRNAs presenting differential expression between NIA-3 and Begonia infected groups: miR-92a and miR-92b-3p from the in vitro approach and miR-206, miR-133a, miR-133b and miR-378 from the in vivo approach. [score:5]
In silico target prediction were performed for those most abundant and DE porcine miRNAs presenting differential expression between NIA-3 and Begonia infected groups: miR-92a and miR-92b-3p from the in vitro approach and miR-206, miR-133a, miR-133b and miR-378 from the in vivo approach. [score:5]
Focusing on those more expressed miRNAs in infected groups and DE between them, we observed that miR-206 (FC = 648), miR-133a (FC = 108), miR-133b (FC = 88) and miR-378 (FC = 5) were more expressed in NIA-3 group (Table S4). [score:5]
miR-206 was previously described to be up-regulated in influenza A virus experimental infected pigs, and it was reported to interact with the antimicrobial protein mucin 1 (MUC1), MyD88 involved in secretion of type I IFN and pro-inflammatory cytokines, and chemokine CCL2 [58]. [score:4]
Focusing on the in vivo approach, there were miRNAs DE between virulent and attenuated strains, particularly more expressed in NIA-3 infected group, like miR-133a (FC = 108), miR-133b (FC = 88), miR-378 (FC = 5) and miR-206 (FC = 648), suggesting that they could work activating those pathways related to the response against the viral infection. [score:3]
miR-206 was not associated to any viral gene. [score:1]
Thus, miR-92a, miR-92b-3p, miR-133a, miR-133b, miR-378 and miR-206 generated a total of 71 significant interactions with 37 SuHV-1 genes (Figure 2). [score:1]
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9
[+] score: 23
Moreover, the myogenic miRNA miR-206 also showed a higher expression level in LR and distinctly different distinct expression patterns between breeds from 49 to 77 dpc. [score:5]
Koutsoulidou A Mastroyiannopoulos NP Furling D Uney JB Phylactou LA Expression of miR-1, miR-133a, miR-133b and miR-206 increases during development of human skeletal muscleBMC Dev. [score:4]
Thus, miR-206 plays an opposite role to miR-186 and showed higher expression in LR than in LT across the studied embryonic stages (G1 up). [score:3]
miR-206 promotes the differentiation of skeletal muscle cells through the inhibition of histone deacetylase 4(HDAC4) which controls muscle differentiation [18]. [score:3]
Among the 35 miRNAs found in the network, miR-133b and miR-206 are known muscle-specific miRNAs that are induced during muscle differentiation and increase the expression of myogenic determination and differentiation factors (such as MEF2A and MEF2C in our network) 24, 25. [score:3]
Though miR-206 was not selected as a DE miRNAs, as it failed to meet the selection criteria, this miRNA indeed showed distinct expression patterns between breeds. [score:3]
Some of miRNAs showed multiple strong interactions with other genes; some of these interactions included well-known, crucial myogenic miRNAs and genes (miR-206, miR-133b, miR135-5p, MEF2A, MEF2C, etc. ) [score:1]
Kim HK Lee YS Sivaprasad U Malhotra A Dutta A Muscle-specific microRNA miR-206 promotes muscle differentiationJ. [score:1]
[1 to 20 of 8 sentences]
10
[+] score: 17
In our study, 19 putative target genes that are regulated by downregulated miRNAs (ssc-miR-101, ssc-miR-7136-5p, ssc-miR-214, ssc-miR-10b, ssc-miR-206, and ssc-miR-320) were found to be involved in the VEGF signaling pathway (Fig 4). [score:7]
Of these, 10 miRNAs (ssc-miR-210, ssc-miR-1343, 12_3058, ssc-miR-676-5p, GL894044.2_23796, 1_4279, 13_5125, ssc-miR-194b-5p, ssc-miR-142-5p, and ssc-miR-421-5p) were up-regulated and 10 (ssc-miR-101, 1_1126, 4_13655, GL892805.1_27591, ssc-miR-320, ssc-miR-7136-5p, ssc-miR-214, ssc-miR-10b, 7_17790, and ssc-miR-206) were downregulated in the TP relative to the YP. [score:7]
Expression levels of five miRNAs (ssc-miR-10b, ssc-miR-206, ssc-miR-214, ssc-miR-320, and ssc-miR-7136-5p) in the cardiac tissues of TPs and YPs were assessed by stem-loop qPCR. [score:3]
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11
[+] score: 12
The distribution of the top most abundant 15 miRNAs in our Solexa sequencing analysis are shown in Table 1. Of these, ssc-miR-206 had the highest reads in the E90 libraries, and ssc-miR-1 was highly expressed at D100. [score:3]
2011; 12(186): doi: 10.1186/1471-2164-12-186 39 Sweetman Dylan, Rathjen Tina, Jefferson Matthew, Wheeler Guy, S Terence G, W Grant N., et al Münsterberg Andrea, Dalmay Tamas, FGF-4 signaling is involved in mir-206 expression in developing somites of chicken embryos. [score:3]
The abundance of ssc-miR-206 is consistent with its well-established activities during skeletal muscle development and its reported role in inducing differentiation in mouse C2C12 cells and during chicken myogenesis [4, 38, 39]. [score:2]
The miR-206 induces C2C12 myoblast differentiation by down -regulating the DNA polymerase α subunit (polyα) [6] and connexin 43 (Cx43) [7]. [score:2]
These two miRNAs were considered to be myomiRs, and the data herein confirmed that ssc-miR-206 and ssc-miR-1 play vital roles in muscle development. [score:1]
Aside from ssc-miR-206 and ssc-miR-1, ssc-miR-378 was the most abundant at E90, followed by ssc-miR-143-3p, ssc-let-7a, ssc-let-7f, ssc-let-7c, ssc-miR-30d, ssc-miR-30a-5p, ssc-miR-10b, ssc-miR-127, ssc-miR-148a, ssc-miR-126, ssc-miR-7i, and ssc-miR-21. [score:1]
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12
[+] score: 11
miR-206 regulates the growth of the teleost tilapia (Oreochromis niloticus) through the modulation of IGF-1 gene expression. [score:4]
Hou et al. [40] showed that ssc-miR-206, ssc-miR-378, and ssc-miR-1 were expressed at extremely high levels in the longissimus dorsi muscles of Tong Cheng pigs. [score:3]
For example, muscle-specific miRNAs (myomiRs), such as miR-1, miR-133a/b, miR-206, and miR-486, were shown to be involved in the regulation of skeletal muscle hypertrophy by modulating the IGF-1–Akt pathway and myostatin signaling pathway [13– 17]. [score:2]
The most abundant miRNA was ssc-miR-206, which was present by more than 4,600,000 TP5M in ten libraries. [score:1]
In the present study, we found that ssc-miR-206, ssc-miR-378, and ssc-miR-1 were ranked 1 [st], 3 [rd], and 4 [th] in abundance among the ten libraries, which is consistent with the previous study. [score:1]
[1 to 20 of 5 sentences]
13
[+] score: 11
Chen et al. [13– 15] previously reported that miR-1 and miR-206 can promote the differentiation of skeletal muscle satellite cells and significantly inhibit their proliferation by decreasing the expression level of Pax7. [score:5]
We have identified several miRNAs that are up-regulated in MSTN [-/-] pigs, and these miRNAs have previously been shown to be involved in myoblast development, including the well-known miR-1, miR-206 [13, 15], and miR-486 [26] (Figure 1A). [score:5]
MiR-148a, miR-206 and miR-214 have been shown to be similar to miR-322/424 and miR-503 [12, 33, 34]. [score:1]
[1 to 20 of 3 sentences]
14
[+] score: 11
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-29a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-208a, hsa-mir-148a, hsa-mir-10a, hsa-mir-181a-2, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-181a-1, hsa-mir-214, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-23b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-206, hsa-mir-1-1, hsa-mir-128-2, hsa-mir-29c, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-148b, hsa-mir-133b, hsa-mir-424, ssc-mir-125b-2, ssc-mir-148a, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-mir-27a, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-128-1, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-503, hsa-mir-411, hsa-mir-378d-2, hsa-mir-208b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-17, ssc-mir-221, ssc-mir-133a-1, ssc-mir-1, ssc-mir-503, ssc-mir-181a-1, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-133b, ssc-mir-29a, ssc-mir-199a-2, ssc-mir-128-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-486-1, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-23b, ssc-mir-148b, ssc-mir-208b, ssc-mir-424, ssc-mir-127, ssc-mir-125b-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-411, ssc-mir-133a-2, ssc-mir-126, ssc-mir-199a-1, ssc-mir-378-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-378j, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, hsa-mir-486-2, ssc-mir-378b
MiR-127, which is located within a CpG island with little research on myogenesis, showed similar expression pattern to ssc-miR-206, up regulated at 35 to 77 dpc and down regulated at 77 dpc to 180 dpn (Figure 5B), providing the possibility for further investigation concerning the function of ssc-miR-127 during muscle development. [score:4]
Similarly, two other myomiRs, miR-133 [21] and miR-206 [23], were highly expressed and ranked the 4 [th] and 6 [th] respectively, while two other miRNAs (miR-378 [24, 25] and miR-143 [25]) ranked the 2 [nd] and 3 [rd] have been identified to participate in the proliferation and differentiation of muscle cells. [score:3]
It’s worth noting that many miRNAs are expressed in a tissue-specific or stage-specific manner [10], and the best-characterized muscle-specific miRNAs (myomiRs [11]) are miR-1, miR-206 and miR-133 families which specifically expressed in cardiac and skeletal muscles. [score:3]
However, Cluster 5 illustrated that ssc-miR-206 and other four muscle-related miRNAs (ssc-miR-126, -148a/b and -15b) continued to decline while ssc-miR-133b and eleven other muscle-related miRNAs (ssc-miR-125b, -128,-181a/b, -199a, -214, -23a, -24, -424, -503 and -7) in Cluster 4 presented a down and then up trend from 77 dpc to 180 dpn, suggesting their different roles played in adult fiber maturation. [score:1]
[1 to 20 of 4 sentences]
15
[+] score: 10
For example, the miR-133 family (ssc-miR-133b, ssc-miR-133a-3p) as well as the let-7 family (ssc-miR-98, ssc-let-7e) was up-regulated in Bama minipigs; in the miR-1/206 family, ssc-miR-1 was up-regulated whilst ssc-miR-206 was down-regulated in Bama minipigs. [score:10]
[1 to 20 of 1 sentences]
16
[+] score: 9
miR-181 was barely detectable in resting muscle cells [47] and miR-206 was only highly expressed in newly formed muscle fibers [51]. [score:3]
In 2006, miR-1 and miR-206 were reported to regulate the myostatin gene which directly impacts muscular hypertrophy [14]. [score:3]
The top ten expressed miRNAs also showed strong signals in the microarrays, with the exception of miR-206, Pearson’s correlation was 0.83 (P < 0.001). [score:3]
[1 to 20 of 3 sentences]
17
[+] score: 8
For example, hsa-miR-133a, hsa-miR-200b, hsa-miR-206, and hsa-miR-218 were considered as tooth tissue-specific miRNAs [4]; eight differentially expressed miRNAs were expressed during morphogenesis and seven were expressed in the incisor cervical loop containing the stem cell niche [1]; the three most highly expressed microRNAs in dental epithelium were identified as mmu-miR-24, mmu-miR-200c, and mmu-miR-205, while mmu-miR-199a-3p and mmu-miR-705 were found in dental mesenchyme [2]; and miR-200 was suggested to play an important role in the formation of incisor cervical loop during stem cell–fueled incisor growth [5]. [score:8]
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18
[+] score: 6
Other miRNAs from this paper: ssc-mir-1
The importance of MYOD1 in regulating the apoptosis of myoblasts via inducing the expression of miR-1 and miR-206 has been demonstrated [35, 36]. [score:4]
Chen J. Tao Y. Li J. Deng Z. Yan Z. Xiao X. Wang D. microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7 J. Cell Biol. [score:2]
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19
[+] score: 5
Such as the myostatin allele in muscle mass QTL interval is characterized by a G to A transition in the 3′ un-translated region (UTR) that creates a target site for miR-1 and miR-206 which are highly expressed in skeletal muscle. [score:5]
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[+] score: 5
For example, inhibition of miRNA-206 in SOD1 [G93A] transgenic mice induced severe atrophy [16]. [score:3]
Previous studies have demonstrated that the myomiRs miR-1, miR-133a/b, miR-206, miR-486, miR-26a, miR-27b, miR-378, miR-148a and miR-181 are highly enriched in skeletal muscle and play a key role in skeletal muscle metabolism [28, 29, 30, 31]. [score:1]
In contrast, miR-1, miR-133a/b and miR-206, as muscle-specific miRNAs, were not the most abundant miRNAs in our data. [score:1]
[1 to 20 of 3 sentences]
21
[+] score: 5
Other miRNAs from this paper: ssc-mir-1
The myostatin (MSTN) allele of Texel sheep was characterized by a G to A transition in the 3′UTR that created a target site for miR-1 and miR-206, which caused the translational inhibition of the MSTN gene and contributed to the muscular hypertrophy of Texel sheep 33. [score:5]
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22
[+] score: 4
Many miRNAs, such as miR-1, miR-133, miR-29, miR-214, miR-206, miR-486, miR-208b, and miR-499 were involved in the regulation of skeletal myogenesis by binding to its target genes 36, 37. [score:4]
[1 to 20 of 1 sentences]
23
[+] score: 4
2011-4179 21821816 7. Lee M. Yoon K. Cho K. Kim K. Jung H. Expression of miR-206 during the initiation of mammary gland development Cell Tissue Res. [score:4]
[1 to 20 of 1 sentences]
24
[+] score: 4
The muscle specifically expressed or highly enriched miRNAs, such as miR-1, miR-133, miR-206 and miR-208, which are responsible for the transcriptional networks involving SRF, MyoD, MEF2 and myocardin, and play central regulatory roles in myoblast proliferation and differentiation during myogenesis [8, 9, 10]. [score:4]
[1 to 20 of 1 sentences]
25
[+] score: 3
Other miRNAs from this paper: ssc-mir-122, ssc-mir-214, ssc-mir-1, ssc-mir-10b
The miR-1 and miR-206 are key mediators in proper skeletal and cardiac muscle development and function, myogenesis during embryonic development and muscle cell differentiation [37]. [score:3]
[1 to 20 of 1 sentences]
26
[+] score: 3
In co-operation with miR-133, miR-206 can repress myoblast fusion by targeting the connexin 43 (Cx43) gap junction channels without altering the Cx43 mRNA level [7]. [score:3]
[1 to 20 of 1 sentences]
27
[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-18a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-21, hsa-mir-23a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-99a, mmu-mir-127, mmu-mir-128-1, mmu-mir-136, mmu-mir-142a, mmu-mir-145a, mmu-mir-10b, mmu-mir-182, mmu-mir-183, mmu-mir-187, mmu-mir-193a, mmu-mir-195a, mmu-mir-200b, mmu-mir-206, mmu-mir-143, hsa-mir-139, hsa-mir-10b, hsa-mir-182, hsa-mir-183, hsa-mir-187, hsa-mir-210, hsa-mir-216a, hsa-mir-217, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-224, hsa-mir-200b, mmu-mir-302a, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-128-1, hsa-mir-142, hsa-mir-143, hsa-mir-145, hsa-mir-127, hsa-mir-136, hsa-mir-193a, hsa-mir-195, hsa-mir-206, mmu-mir-19b-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-31, mmu-mir-92a-2, mmu-mir-96, mmu-mir-98, hsa-mir-200c, mmu-mir-17, mmu-mir-139, mmu-mir-200c, mmu-mir-210, mmu-mir-216a, mmu-mir-219a-1, mmu-mir-221, mmu-mir-222, mmu-mir-224, mmu-mir-19b-1, mmu-mir-92a-1, mmu-mir-128-2, hsa-mir-128-2, mmu-mir-217, hsa-mir-200a, hsa-mir-302a, hsa-mir-219a-2, mmu-mir-219a-2, hsa-mir-363, mmu-mir-363, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-371a, hsa-mir-18b, hsa-mir-20b, hsa-mir-452, mmu-mir-452, ssc-mir-106a, ssc-mir-145, ssc-mir-216-1, ssc-mir-217-1, ssc-mir-224, ssc-mir-23a, ssc-mir-183, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-128-1, ssc-mir-136, ssc-mir-139, ssc-mir-18a, ssc-mir-21, hsa-mir-146b, hsa-mir-493, hsa-mir-495, hsa-mir-497, hsa-mir-505, mmu-mir-20b, hsa-mir-92b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, hsa-mir-671, mmu-mir-216b, mmu-mir-671, mmu-mir-497a, mmu-mir-495, mmu-mir-146b, mmu-mir-708, mmu-mir-505, mmu-mir-18b, mmu-mir-493, mmu-mir-92b, hsa-mir-708, hsa-mir-216b, hsa-mir-935, hsa-mir-302e, hsa-mir-302f, ssc-mir-17, ssc-mir-210, ssc-mir-221, mmu-mir-1839, ssc-mir-146b, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-128-2, ssc-mir-143, ssc-mir-10b, ssc-mir-23b, ssc-mir-193a, ssc-mir-99a, ssc-mir-98, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-142, ssc-mir-497, ssc-mir-195, ssc-mir-127, ssc-mir-222, ssc-mir-708, ssc-mir-935, ssc-mir-19b-2, ssc-mir-19b-1, ssc-mir-1839, ssc-mir-505, ssc-mir-363-1, hsa-mir-219b, hsa-mir-371b, ssc-let-7a-2, ssc-mir-18b, ssc-mir-187, ssc-mir-218b, ssc-mir-219a, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-31, ssc-mir-182, ssc-mir-216-2, ssc-mir-217-2, ssc-mir-363-2, ssc-mir-452, ssc-mir-493, ssc-mir-671, mmu-let-7k, ssc-mir-7138, mmu-mir-219b, mmu-mir-216c, mmu-mir-142b, mmu-mir-497b, mmu-mir-935, ssc-mir-9843, ssc-mir-371, ssc-mir-219b, ssc-mir-96, ssc-mir-200b
adj ssc-miR-21 -1.1788 1.45E-02 1.68E-02 -2.4642 2.07E-04 3.85E-04 ssc-miR-143-3p -1.1940 1.40E-02 1.67E-02 -2.7004 2.27E-05 5.34E-05 ssc-miR-145-3p -1.2289 2.47E-02 2.68E-02 -2.6837 6.34E-04 1.10E-03 ssc-miR-505 -1.3657 2.68E-02 2.82E-02 -2.1577 4.16E-02 4.16E-02 ssc-miR-98 -1.5185 3.46E-03 5.15E-03 -2.8061 7.55E-05 1.55E-04 ssc-miR-139-3p -1.6685 2. 54E-02 2.71E-02 -2.5158 1.69E-02 1.93E-02 ssc-miR-23b -1.7157 3.70E-03 5.42E-03 -2.3687 8.39E-03 1.10E-02 ssc-miR-224 -1.8515 1.41E-02 1.67E-02 -2.5778 1.95E-02 2.19E-02 ssc-miR-23a -1.8753 3.40E-03 5.15E-03 -2.4676 1.00E-02 1.24E-02 ssc-miR-143-5p -1.9243 1.15E-04 2.60E-04 -3.9943 1.25E-09 5.88E-09 ssc-miR-139-5p -2.1198 2.01E-02 2.24E-02 -3. 2644 1.01E-02 1.24E-02 ssc-miR-222 -2.2666 2.58E-07 1.02E-06 -2.6019 2.34E-05 5.35E-05 ssc-miR-671-5p -2.3068 1.15E-02 1.47E-02 -2.7986 3.86E-02 3.92E-02 ssc-miR-9843-3p -2.3507 9.68E-04 1.87E-03 -4.7281 5.90E-05 1.31E-04 ssc-miR-145-5p -2.7059 2.08E-03 3.50E-03 -4.3459 7.18E-05 1.51E-04 ssc-miR-221-5p -2.7136 3.21E-07 1.21E-06 -1.9513 3.02E-02 3. 22E-02 ssc-miR-221-3p -2.9643 8.31E-11 5.47E-10 -2.1967 1.74E-03 2.90E-03 ssc-miR-708-5p -4.0615 2.31E-06 7.60E-06 -2.8238 6.43E-03 8.72E-03 ssc-miR-193a-3p -4.1933 2.39E-07 1.02E-06 -4.3848 2.87E-07 9.18E-07 ssc-miR-193a-5p -4.1933 2.39E-07 1.02E-06 -7.1423 2.32E-12 1.33E-11 ssc-miR-452 -4.3025 5.55E-11 3.99E-10 -2.2057 1.53E-02 1.77E-02 ssc-miR-206 -5.3001 6. 39E-09 3.37E-08 -6.2200 3.10E-09 1.38E-08 10.1371/journal. [score:1]
adj ssc-miR-21 -1.1788 1.45E-02 1.68E-02 -2.4642 2.07E-04 3.85E-04 ssc-miR-143-3p -1.1940 1.40E-02 1.67E-02 -2.7004 2.27E-05 5.34E-05 ssc-miR-145-3p -1.2289 2.47E-02 2.68E-02 -2.6837 6.34E-04 1.10E-03 ssc-miR-505 -1.3657 2.68E-02 2.82E-02 -2.1577 4.16E-02 4.16E-02 ssc-miR-98 -1.5185 3.46E-03 5.15E-03 -2.8061 7.55E-05 1.55E-04 ssc-miR-139-3p -1.6685 2. 54E-02 2.71E-02 -2.5158 1.69E-02 1.93E-02 ssc-miR-23b -1.7157 3.70E-03 5.42E-03 -2.3687 8.39E-03 1.10E-02 ssc-miR-224 -1.8515 1.41E-02 1.67E-02 -2.5778 1.95E-02 2.19E-02 ssc-miR-23a -1.8753 3.40E-03 5.15E-03 -2.4676 1.00E-02 1.24E-02 ssc-miR-143-5p -1.9243 1.15E-04 2.60E-04 -3.9943 1.25E-09 5.88E-09 ssc-miR-139-5p -2.1198 2.01E-02 2.24E-02 -3. 2644 1.01E-02 1.24E-02 ssc-miR-222 -2.2666 2.58E-07 1.02E-06 -2.6019 2.34E-05 5.35E-05 ssc-miR-671-5p -2.3068 1.15E-02 1.47E-02 -2.7986 3.86E-02 3.92E-02 ssc-miR-9843-3p -2.3507 9.68E-04 1.87E-03 -4.7281 5.90E-05 1.31E-04 ssc-miR-145-5p -2.7059 2.08E-03 3.50E-03 -4.3459 7.18E-05 1.51E-04 ssc-miR-221-5p -2.7136 3.21E-07 1.21E-06 -1.9513 3.02E-02 3. 22E-02 ssc-miR-221-3p -2.9643 8.31E-11 5.47E-10 -2.1967 1.74E-03 2.90E-03 ssc-miR-708-5p -4.0615 2.31E-06 7.60E-06 -2.8238 6.43E-03 8.72E-03 ssc-miR-193a-3p -4.1933 2.39E-07 1.02E-06 -4.3848 2.87E-07 9.18E-07 ssc-miR-193a-5p -4.1933 2.39E-07 1.02E-06 -7.1423 2.32E-12 1.33E-11 ssc-miR-452 -4.3025 5.55E-11 3.99E-10 -2.2057 1.53E-02 1.77E-02 ssc-miR-206 -5.3001 6. 39E-09 3.37E-08 -6.2200 3.10E-09 1.38E-08 10.1371/journal. [score:1]
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[+] score: 2
These miRNAs have previously been identified in pig skeletal muscle, including Nielsen et al. [11], where ssc-miR-1 and ssc-miR-206 were the two most highly abundant miRNAs identified from sequencing LD samples from seven 1.5–2-year-old Danish Landrace/Yorkshire crossbred pigs. [score:1]
The five most abundant miRNAs represented 47.9% of the total cpm in the dataset including ssc-miR-1, ssc-miR-133a­3p, ssc­miR-378, ssc-miR-206, and ssc-miR-10b. [score:1]
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[+] score: 2
Using miRNA microarray analysis and RT-PCR, some researchers have found that miR-24, miR-31, miR-140, miR-141, miR-205, miR-200c, miR-875-5p, miR-455, miR-689, miR-711, and miR-720 may regulate tooth epithelial stem cell differentiation [6], [7]; others identified miR-133a, miR-200b, miR-206, and miR-218 as tooth-specific miRNAs, and that miR-141, miR-199b*, miR-200a, miR-200b, miR-200c, and miR-429 likely play a role in the renewal and differentiation of adult stem cells during stem cell-fueled incisor growth [8], [9]. [score:2]
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30
[+] score: 1
For example, in the Texel sheep, the creation of miR-1 and miR-206 binding sites by a SNP in the 3′ UTR region of the GDF8 shows perfect association with sheep hyper-muscularity [20], [21]. [score:1]
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