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16 publications mentioning bta-mir-142

Open access articles that are associated with the species Bos taurus and mention the gene name mir-142. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 149
Down-regulated miR-20a-5p was found to potentially target eight up-regulated DEG (ELK4 (ETS transcription factor), ELOVL6 (fatty acid elongase 6), ETV1 (ETS variant 1), KDM6B (lysine demethylase 6B), KIAA1524, LONP2 (lon peptidase 2, peroxisomal), M6PR (mannose-6-phosphate receptor, cation dependent), USP12 (ubiquitin specific peptidase 12)) in LF-SO cows and miR-142-5p was found to potentially target four up-regulated DEG (ELK4, ELOVL6, ETV1, PIK3CD (phosphatidylinositol 3-kinase catalytic delta polypeptide)). [score:14]
miRNA Gene symbol Gene name Functional category miR-20a-5p, ELK4 ETS-domain protein Replication/Transcription/Translation miR-142-5p (SRF accessory protein 1) miR-20a-5p, ELOVL6 ELOVL fatty acid elongase 6 Cellular lipid metabolism & transport miR-142-5p miR-20a-5p, ETV1 ETS variant 1 Replication/Transcription/Translation miR-142-5p miR-20a-5p KDM6B Lysine (K)-specific demethylase 6B Replication/Transcription/Translation miR-20a-5p KIAA1524 KIAA1524 ortholog Cell cycle,cell growth, proliferation, differentiation & death miR-20a-5p LONP2 Lon peptidase 2n peroxisomal Cellular protein metabolism & transport miR-20a-5p M6PR Mannose-6-phophatase receptor (cation dependent) Cellular carbohydrate metabolism & transport miR-20a-5p USP12 Ubiquitin specific peptidase 12 Cellular protein metabolism & transport miR-142-5p PIK3CD Phosphoinositide-3-kinase, catalytic, delta polypeptide Immune, inflammatory and stress response Impact of miR-20a-5p and miR-142-5p on the expression of ELOVL6, gene involved in lipid metabolismTo investigate whether miR-20a-5p and/or miR-142-5p could regulate the expression of ELOVL6 in mammary epithelial cells, miRNAs were over-expressed individually or together using miRNA mimics in culture bovine mammary epithelial monolayers (BME-UV1). [score:12]
However, the in vivo down-regulation of miR-20a-5p and miR-142-5p, and the up-regulation of ELOVL6, were observed in a context of lactation, which was not reproduced in cultured cells where the cellular context may influence miRNA function [73]. [score:7]
miRNA Gene symbol Gene name Functional category miR-20a-5p, ELK4 ETS-domain protein Replication/Transcription/Translation miR-142-5p (SRF accessory protein 1) miR-20a-5p, ELOVL6 ELOVL fatty acid elongase 6 Cellular lipid metabolism & transport miR-142-5p miR-20a-5p, ETV1 ETS variant 1 Replication/Transcription/Translation miR-142-5p miR-20a-5p KDM6B Lysine (K)-specific demethylase 6B Replication/Transcription/Translation miR-20a-5p KIAA1524 KIAA1524 ortholog Cell cycle,cell growth, proliferation, differentiation & death miR-20a-5p LONP2 Lon peptidase 2n peroxisomal Cellular protein metabolism & transport miR-20a-5p M6PR Mannose-6-phophatase receptor (cation dependent) Cellular carbohydrate metabolism & transport miR-20a-5p USP12 Ubiquitin specific peptidase 12 Cellular protein metabolism & transport miR-142-5p PIK3CD Phosphoinositide-3-kinase, catalytic, delta polypeptide Immune, inflammatory and stress response ABCA1: ATP-Binding Cassette subfamily A (ABC1) member1, BTN1A1: BuTyrophiliN subfamily 1, member A1, LPIN1: LiPIN1, PPARγ: Peroxisome Proliferator-Activated Receptor gamma, PPARGC1B: Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Beta, SCD: Steroyl-CoA Desaturase, VLDLR: Very Low Density Lipoprotein Lipase Receptor. [score:7]
Both miR-20a-5p and miR-142-5p are predicted to target ELK4 and ETV1 which are known to be involved in replication, transcription and translation. [score:5]
It can nevertheless be hypothesized that a weaker expression of miR-20a-5p and miR-142-5p may have contributed to the increased expression of ELOVL6 in the mammary glands of cows receiving the LF-SO diet. [score:5]
Among them, twenty-three predicted targets for miR-20a-5p and miR-142-5p have been identified, using DIANA software, which three were targeted by the two miRNAs. [score:5]
Members of the PPAR family such as PPARα, PPARγ and the co-activator PPARGC1B (peroxisome proliferative activated receptor, gamma, coactivator 1 beta), are known to be transcription factors involved in the control of genes coding for lipogenic enzymes, are targeted by miR-142-5p, and are predicted to be targeted by miR-20a-5p [62]. [score:5]
0185511.g005 Fig 5Effect of miR-20a-5p and miR-142-5p over -expression on ELOVL6 expression in BME-UV1 cells. [score:5]
Effect of miR-20a-5p and miR-142-5p over -expression on ELOVL6 expression in BME-UV1 cells. [score:5]
Interestingly, both miR-20a-5p and miR-142-5p potentially target ELOVL6, a member of the family of fatty acid elongases [67], which are involved in lipid metabolism and were over-expressed in our LF-SO samples. [score:5]
**0.01miR-142-5p are predicted to target genes differentially expressed by sunflower oilIn order to investigate the functional role of miR-20a-5p and miR-142-5p, computational applications were used to predict their targets. [score:5]
miR-20a-5p and miR-142-5p are predicted to target genes differentially expressed by sunflower oil. [score:5]
To investigate whether miR-20a-5p and/or miR-142-5p could regulate the expression of ELOVL6 in mammary epithelial cells, miRNAs were over-expressed individually or together using miRNA mimics in culture bovine mammary epithelial monolayers (BME-UV1). [score:4]
Among the eight studied miRNAs, RT-qPCR validations confirmed that miR-20a-5p (p = 0.08) and miR-142-5p (p = 0.03) were significantly down-regulated by sunflower oil supplementation. [score:4]
Consequently, miR-20a-5p and miR-142-5p may impact lipid metabolism in response to oil supplementation as a function of their putative targets. [score:3]
Predicted targets of miR-20a-5p and miR-142-5p involved in the lipid metabolism. [score:3]
Both our results and those obtained in adipose tissues suggest that the expression of miR-142-5p is strongly influenced by dietary lipids. [score:3]
Then among the eight candidate miRNAs highlighted by the RNA sequencing study, it was confirmed, by RT-qPCR on samples from 11 cows (each cow receiving each diet), that the expression of miR-20a-5p and miR-142-5p was decreased by sunflower oil supplementation. [score:3]
Predicted targets of miR-20a-5p and miR-142-5p among DEG previously identified by microarrays. [score:3]
As seed regions are conserved between human and cattle for miR-20a-5p and miR-142-5p, putative targets for these miRNA were predicted with a high degree of accuracy based on DIANA-microT-CDS v5.0 [53]. [score:3]
Impact of miR-20a-5p and miR-142-5p on the expression of ELOVL6, gene involved in lipid metabolism. [score:3]
MiR-20a-5p and miR-142-5p were transfected separately or together for 48h in BME-UV1 cells, and the expression of ELOVL6 was quantified by RT-qPCR. [score:3]
In addition, a high fat diet given in the form of flaxseed to steers for 14 weeks led to 185-fold and 968-fold increases in miR-142-5p expression in subcutaneous and visceral adipose tissues, respectively [42]. [score:3]
Indeed, in the white adipose tissue of mice receiving a high fat diet for 5 months, miR-142-5p was significantly up-regulated when compared with the findings in control mice [59]. [score:3]
miRNA Gene symbol Gene name miR-20a-5p ABCA1 ATP-Binding cassette, sub -family A (ABC1), member 1 APOBEC4 Apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 4 (putative) APP Amyloid beta (A4) precursor protein BTN1A1 Butyrophilin, subfamily 1, member 1 ELOVL6 ELOVL fatty acid elongase 6 LDLR Low density lipoprotein receptor LPIN1 Lipin 1 PPARA Peroxisome proliferator-activated receptor alpha PPARG Peroxisome proliferator-activated receptor gamma PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 beta SCD5 Steroyl-CoA desaturase VLDLR Very low density lipoprotein receptor miR-142-5p ABCA1 ATP-Binding cassette, sub -family A (ABC1), member 1 ACADL Acyl-CoA dehydrogenase, long chain ACAT1 Acetyl-CoA acetyltransferase 1 ACSL1,6 Acyl-CoA synthetase long-chain family member 1,6 ELOVL4, 5, 6 ELOVL fatty acid elongase 4,5,6 LRP2, 4 Low density lipoprotein receptor related-protein 2, 4   PPARGC1B Peroxisome proliferator-activated receptor gamma, coactivator 1 betamiRNA’s targets were predicted using Diana microT-CDS (http://diana. [score:3]
Sunflower oil supplementation altered the expression of miR-20a-5p and miR-142-5p. [score:3]
Interestingly, among the predicted targets of miR-20a-5p and miR-142-5p are nine DEG previously identified in the same samples using previous transcriptomic analysis [46] (Table 3). [score:3]
Although the response of miR-20a-5p to lipid supplementation has not yet been described, the change in the expression of miR-142-5p observed here is in line with previous reports regarding adipose tissues. [score:3]
Otherwise, miR-142-5p is also predicted to target two isoforms of the ACSL (acyl-CoA synthetase long-chain) enzyme (Table 2). [score:3]
ELOVL6 expression after transfection of miR-20a-5p and miR-142-5p (B) or miR-1-3p (C). [score:3]
0185511.g004 Fig 4Predicted binding site of miR-20a-5p, miR-142-5p and miR-1-3p in the 3’UTR of ELOVL6. [score:1]
No significant changes were obtained in cells transfected with miR-20a-5p and miR-142-5p, either separately or together (Fig 5B). [score:1]
Predicted binding site of miR-20a-5p, miR-142-5p and miR-1-3p in the 3’UTR of ELOVL6. [score:1]
We can hypothesize that the interaction predicted between miR-20a-5p and miR-142-5p in BME-UV1 cells does not occur or that the miRNAs affects the protein level. [score:1]
Putative targets of miR-20-5p and miR-142-5p among these DEG were investigated. [score:1]
In order to investigate the functional role of miR-20a-5p and miR-142-5p, computational applications were used to predict their targets. [score:1]
To evaluate the role of miR-20a-5p and miR-142-5p, these miRNAs were over-expressed in bovine mammary epithelial cell line. [score:1]
Eight miRNAs (miR-15a-5p, miR-17-5p, miR-20a-5p, miR-33a-3p, miR-126-3p, miR-181a-5p, miR-142-5p and miR-223-3p; Supplementary S1 Table) were chosen for further study on the basis of their ranking and their function highlighted in the literature. [score:1]
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2
[+] score: 30
The mRNA expression of actin -binding protein PFN2, the potential target of miR-17-5p, miR-19a, miR-30b-5p, miR-142-5p, miR-301a, and miR-2478, also tended to be downregulated in the grazing cattle compared to the grain-fed cattle (P = 0.064), as did the potential miR-29b targets DNAJB2 (P = 0.021) and COL3A1 (P = 0.100). [score:9]
One of the lipogenic genes, SCD1, which is predicted as a target of miR-98, miR-142-5p, and miR-2478, showed higher expression in the grain-fed than in the grazing cattle (P = 0.038, Fig 4). [score:5]
Next, we analyzed the muscle tissue expression of the 11 miRNAs that differed significantly between the groups (miR-10b, miR-17-5p, miR-19a, miR-29b, miR-30b-5p, miR-98, miR-142-5p, miR-301a, miR-374b, miR-425-5p, and miR-652) to determine whether or not c-miRNA expression was associated with that of skeletal muscle tissue miRNAs. [score:5]
The expression of PTEN, the potential target of miR-10b, miR-17-5p, miR-19a, miR-29b, miR-30b-5p, miR-142-5p, miR-301a, miR-652, and miR-2478, was lower in the grazing cattle than in the grain-fed cattle (P = 0.011). [score:5]
In the present study, muscle PTEN and PFN2 were the predicted targets of most of the altered c-miRNAs (miR-17-5p, miR-19a, miR-30b-5p, miR-142-5p, miR-301a) between the feeding conditions, as well as miR-10b. [score:3]
[1]log2(G/H): the value of log2(grazing/grain-fed)We then conducted qPCR for the contents of c-miRNAs of interest, not only to validate the results of microarray analysis for those 13 miRNAs and the miRNAs below the top 20 in SAM analysis (miR-29b, miR-197), but also to further explore potential feeding -induced miRNAs among adipose-enriched (miR-15a, miR-19a, miR-27b, miR-92a, miR-103, miR-142-5p), plasma-enriched (miR-2412, miR-2478), and bovine-specific (miR-2284x and miR-2295) miRNAs [31]. [score:1]
In contrast, the grazing cattle showed significantly lower contents of miR-17-5p (P = 0.031), miR-19a (P = 0.007), miR-29b (P = 0.021), miR-30b-5p (P = 0.035), miR-98 (P = 0.006), miR-142-5p (P = 0.013), miR-301a (P = 0.005), miR-374b (P = 0.016), miR-425-5p (P = 0.010), and miR-652 (P = 0.046). [score:1]
[1]log2(G/H): the value of log2(grazing/grain-fed) We then conducted qPCR for the contents of c-miRNAs of interest, not only to validate the results of microarray analysis for those 13 miRNAs and the miRNAs below the top 20 in SAM analysis (miR-29b, miR-197), but also to further explore potential feeding -induced miRNAs among adipose-enriched (miR-15a, miR-19a, miR-27b, miR-92a, miR-103, miR-142-5p), plasma-enriched (miR-2412, miR-2478), and bovine-specific (miR-2284x and miR-2295) miRNAs [31]. [score:1]
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3
[+] score: 16
Other miRNAs from this paper: bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-30d, bta-mir-99a, bta-mir-145, bta-mir-181a-2, bta-mir-199a-1, bta-mir-27b, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-181c, bta-mir-191, bta-mir-199b, bta-mir-214, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-let-7a-1, bta-mir-150, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-mir-34c, bta-mir-363, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-195, bta-mir-34a, bta-mir-365-1, bta-mir-99b, bta-mir-100, bta-mir-129-1, bta-mir-129-2, bta-mir-130a, bta-mir-130b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-143, bta-mir-146b, bta-mir-146a, bta-mir-155, bta-mir-181d, bta-mir-182, bta-mir-183, bta-mir-184, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-199a-2, bta-mir-212, bta-mir-26a-1, bta-mir-28, bta-mir-29d, bta-mir-32, bta-mir-335, bta-mir-338, bta-mir-339a, bta-mir-346, bta-mir-365-2, bta-mir-378-1, bta-mir-383, bta-mir-409a, bta-mir-449a, bta-mir-449b, bta-mir-449c, bta-mir-592, bta-mir-708, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-1271, bta-mir-1249, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2285a, bta-mir-2285d, bta-mir-2285b-1, bta-mir-2332, bta-mir-199c, bta-mir-2389, bta-mir-2285c, bta-mir-2404-1, bta-mir-449d, bta-mir-2411, bta-mir-2446, bta-mir-339b, bta-mir-2404-2, bta-mir-2483, bta-mir-424, bta-mir-378-2, bta-mir-409b, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2285n-7, bta-mir-2285k-2, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2285k-5, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2285ae, bta-mir-378d, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Given the gene expression and/or translation repression role of miRNAs, increased level of miR-142-5p in granulosa cells of DF may suggest downregulation of axon guidance by Slit/Robo pathway. [score:8]
This may suggest that miR-142-5p could be an important candidate for granulosa cell survival by targeting genes involving in guidance by Slit/Robo. [score:3]
For instance, the miRNA-gene interaction and pathway analysis showed that the target genes of by miR-142-5p, namely, ENAH, ROBO1, Rho GTPases (RHOA, RHOQ), NEO1 were found to be involved in axon guidance mediated by Slit/Robo. [score:3]
Among miRNAs increased at day 7 of the estrous cycle, bta-miR-2389, bta-miR-29d, bta-miR-363, bta-miR-1249, bta-miR-338, bta-miR-129-3p, bta-miR-129-5p, bta-miR-129, bta-miR-142-3p, bta-miR-449b, bta-miR-2285t and bta-miR-346 were not detected at day 3 (Figure 9A). [score:1]
Indeed, the anti-apoptosis role of miR-142-5p has been previously described [64]. [score:1]
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[+] score: 11
miR-223 has been shown to up-regulate miR-142 expression through transcription factors (LMO2-L/-S isoforms and CEBP-β) [83]. [score:6]
Sun W. Shen W. Yang S. Hu F. Li H. Zhu T. -H. MiR-223 and miR-142 attenuate hematopoietic cell proliferation, and miR-223 positively regulates miR-142 through LMO2 isoforms and CEBP-βCell. [score:2]
Functional analysis of miR-142-3p indicated its importance in regulating signal to balance cell cycle processes such as balance of cell proliferation and differentiation of mesenchymal cells [82]. [score:2]
Interestingly, miR-223 and miR-142 showed strong interaction in the TURQUOISE module. [score:1]
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5
[+] score: 10
14/26s highly expressed in the HM fraction (let-7d-5p, miR-103a-3p, miR-142-3p, miR-17-5p, miR-18a-5p, miR-196a-5p, miR-20a-5p, miR-24-1-5p, miR-26a-5p, miR-301a-3p, miR-30b-5p, miR-34b-5p, miR-34c-5p, miR-378a-3p) and 7/14s highly expressed in the LM fraction (miR-10b-5p, miR-122-5p, miR-1-3p, miR-184, miR-486-5p, miR-7-5p, miR-99b-5p) were predicted to target 327 and 281 experimentally observed genes, respectively. [score:7]
The relative expression in HM and LM fractions of several of these knowns, including bta-miR-103, bta-miR-30b-5p, bta-miR-17-5p, bta-miR-106b, bta-miR-142-3p, bta-miR-34b, bta-miR-18a, bta-miR-34c, bta-miR-455-5p, bta-miR-10b, bta-miR-99b, bta-miR-1246, bta-miR-99a-5p, and bta-miR-1388-5p, was consistent with the relative abundance of their homologouss, observed in the normal vs abnormal sperm. [score:3]
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[+] score: 9
We selected 8 miRNAs that were reported to be upregulated (miR-145, miR-143, miR-99a-5p) or downregulated (miR-155 and miR-142-3p, miR-132, miR-378) in follicular relative to luteal tissues of ruminants [19, 21] or differentially expressed in endometrium during the human menstrual cycle (miR-31) [48– 50]. [score:9]
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7
[+] score: 8
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-20a, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
They detected let-7a-3p as a regulator of zonadhesin-like and growth hormone 2 gene, miR-142-5p as a regulator of heparin -binding growth factor 1, and miR-144 as a regulator of the growth factor receptor-bound protein 2. They also found that miR-430 regulates the transforming growth factor-beta -induced protein ig-h3, miR-451 blocks the anti-dorsalizing morphogenic protein, and miR-1594 activates both titin-cap (telethonin)-like mRNA and growth hormone receptor isoform 2. All of these miRNAs and targets are associated with growth and developmental processes. [score:8]
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8
[+] score: 8
Bta-miR-423-5p which was found to be a whey enriched miRNA by DE analysis showed the highest expression in whey (p < 0.05) while the four cell enriched miRNAs (bta-miR-142-5p, miR-146a, miR-221, miR-223) were most highly expressed in milk cells than in the other two milk fractions (p < 0.05). [score:5]
Statistically, bta-miR-142-5p and miR-146a demonstrated a comparable expression level between milk cell and mammary gland tissue. [score:3]
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9
[+] score: 8
Among them, the more expressed let-7, miR-142, miR-196b and lower expressed miR-320a have been reported to regulate lipid metabolism. [score:6]
In the present study, we found that the expression levels of miR-142-3p, miR-379 and miR-196a were higher in the adipose tissue of Wagyu cattle compared with Holstein (Table 2). [score:2]
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10
[+] score: 5
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26b, hsa-mir-27a, hsa-mir-31, hsa-mir-33a, hsa-mir-99a, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-147a, hsa-mir-34a, hsa-mir-182, hsa-mir-199a-2, hsa-mir-212, hsa-mir-221, hsa-mir-224, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-132, hsa-mir-142, hsa-mir-145, hsa-mir-152, hsa-mir-153-1, hsa-mir-153-2, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-127, hsa-mir-134, hsa-mir-200c, hsa-mir-106b, hsa-mir-361, hsa-mir-148b, hsa-mir-20b, hsa-mir-410, hsa-mir-202, hsa-mir-503, hsa-mir-33b, hsa-mir-643, hsa-mir-659, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-221, bta-mir-26b, bta-mir-27a, bta-mir-99a, bta-mir-125a, bta-mir-125b-1, bta-mir-145, bta-mir-199a-1, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-127, bta-mir-20b, bta-let-7d, bta-mir-132, bta-mir-148b, bta-mir-200c, bta-mir-22, bta-mir-23a, bta-mir-29b-2, bta-mir-361, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-34a, hsa-mir-708, hsa-mir-147b, hsa-mir-877, hsa-mir-940, hsa-mir-548j, hsa-mir-302e, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-100, bta-mir-106b, bta-mir-130a, bta-mir-134, bta-mir-147, bta-mir-152, bta-mir-153-1, bta-mir-153-2, bta-mir-182, bta-mir-24-1, bta-mir-199a-2, bta-mir-202, bta-mir-212, bta-mir-224, bta-mir-33a, bta-mir-33b, bta-mir-410, bta-mir-708, bta-mir-877, bta-mir-940, bta-mir-29b-1, bta-mir-148c, bta-mir-503, bta-mir-148d
This study demonstrated that eight miRNAs (miR-503, miR-21, miR-29b, miR-142-3p, miR-34a, miR-152, miR-25 and miR-130a) were highly expressed, while nine miRNAs (miR-125a, miR-199a-3p, miR-125b, miR-99a, let-7c, miR-145, miR-31, miR-202 and miR-27b) were expressed at lower level between the follicular and luteal stages in ovine ovarian tissues. [score:5]
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[+] score: 5
For completeness, our qPCR analyses also included 1 gene (MYD88), which interactions with 2 miRNAs were experimentally validated but not computationally predicted in bovine (Table 3 and Fig.  3), and 2 genes, IGF1 and PAPPA, which were computationally predicted bovine targets of 2 miRNAs (miR-222 and miR-378) and 3 miRNAs (miR-142-5p, miR-150, and miR-378), respectively, but none of which were experimentally validated in any species, ie, they were present in TargetScan but not in miRTarBase (Table 3 and Fig.  3). [score:5]
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12
[+] score: 4
Co-transfection of pre-miR-142 (control miRNA; not complementary to the 3 [′]-UTR of MSTN) with the wild-type MSTN 3 [′]-UTR construct did not alter the luciferase activity, indicating that miR-27b specifically targeted MSTN (Figure 4B). [score:3]
Cells were seeded at 1x10 [4] cells per well of a 24-well plate and grown for 24 h. A total of 300 ng of the appropriate psicheck-2 luciferase reporter construct was cotransfected with 15 nM final concentration of either double-stranded RNA oligonucleotides designed to mimic miR-27b and miR-142 molecules (Ambion) using Metafectene (Biontex Laboratories, Planegg, Germany) according to the manufacturer’s protocol. [score:1]
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[+] score: 4
PRLR is the putative target of miR-142, miR-23, miR-374b, miR-30a and miR-27b and plays a function in MG development together with prolactin. [score:4]
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[+] score: 4
Other miRNAs from this paper: bta-mir-26a-2, bta-mir-101-2, bta-mir-148a, bta-mir-30d, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-30b, bta-mir-30e, bta-mir-148b, bta-mir-186, bta-mir-191, bta-mir-22, bta-mir-30a, bta-mir-150, bta-mir-30c, bta-mir-101-1, bta-mir-141, bta-mir-146a, bta-mir-223, bta-mir-26a-1, bta-mir-30f, bta-mir-181a-1, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-1388, bta-mir-2898, bta-mir-2904-1, bta-mir-2904-2, bta-mir-2904-3, bta-mir-2284w, bta-mir-2284x, bta-mir-148c, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2284y-7, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-1842, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-148d, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
In particular, bta-miR-142-5p, and −223 are potential biomarkers for early detection of bacterial infection of the mammary gland. [score:1]
For example, we detected increases of bta-miR-142-5p and −223 in milk exosomes 48 h post-infection, while both lactating mammary epithelium [66] and bovine monocytes [19] under infection challenge with S. uberis also were found to increase levels of miR-223. [score:1]
Lawless and colleagues also found significant increases in miR-142-5p in mammary monocytes post-infection [19]. [score:1]
MiR-142 has also been shown to be abundant in T cells [69], which implied its role as an immune-relevant miRNA while its function remains unclear. [score:1]
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[+] score: 4
Six miRNAs (miR-16b, miR-19a, miR-106, miR142–5 p, miR-196a and miR-2454) had the expression in agreement between qRT-PCR and microarray, however the other six (miR92a, miR-92b, miR-101, miR-103, miR-296 and miR-2368*) had divergent results. [score:3]
Increases ranged from a low of 2.62 fold in subcutaneous adipose tissue and 8.94 fold in the visceral adipose tissue for miR-92b, to a high of 185.11 in subcutaneous adipose tissue and 968.77 fold in the visceral adipose tissue for miR-142–5p. [score:1]
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[+] score: 1
In the PBMC dataset, miR-142-3p was identified in the quantile-normalized dataset, but was not identified under any other normalization strategy (Table 2). [score:1]
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