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2 publications mentioning cel-mir-248

Open access articles that are associated with the species Caenorhabditis elegans and mention the gene name mir-248. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 36
Other miRNAs from this paper: cel-mir-1, cel-mir-71, cel-mir-72, cel-mir-74, cel-mir-228, cel-mir-255
To knockdown mir-248 expression, we made an anti- mir248 transgene expressing short hairpin RNA (shRNA), consisting of both sense and antisense sequences of mir-248, in AWC. [score:6]
Unlike the highly penetrant 2AWC [ON] phenotype caused by mir-71 overexpression, mir-248 overexpression generated a mixed weak phenotype of 2AWC [ON] and 2AWC [OFF] (Figure S1B). [score:5]
These results suggest that mir-71 may not act redundantly with mir-248 to regulate tir-1 expression in AWC asymmetry. [score:4]
The anti- mir-248 transgene caused an AWC phenotype similar to mir-248(OE) (data not shown), suggesting that the effect of the anti -mir-248 transgene on AWC asymmetry is not through knockdown of mir-248 but mainly due to overexpression of sense mir-248 in the shRNA construct. [score:4]
In addition to mir-71, mir-248 was also predicted to target tir-1 by three programs (Figure S1A). [score:3]
Since mir-248 mutants are not available, we analyzed the effect of mir-248 overexpression on AWC asymmetry. [score:3]
Based on these criteria, we identified six potential miRNAs (mir-71, mir-72, mir-74, mir-228, mir-248, mir-255) predicted to target unc-2, unc-43, tir-1, nsy-1, and sek-1 (Figure S1A). [score:3]
mir-71 and mir-248 have different predicted target sites in the tir-1 3′ UTR. [score:3]
To test whether mir-71 and mir-248 have a synergistic effect on AWC symmetry, we made transgenic animals overexpressing both mir-71 and mir-248 in AWCs. [score:3]
The 2AWC [ON] phenotype was not significantly higher in mir-71(OE); mir-248(OE) animals than in mir-71(OE) (data not shown). [score:1]
To make shRNA anti- mir-248 (mir-248IR), the sense and antisense oligos, each consisting of mir-248 sense (24 nt) and antisense (24 nt) sequences that flank a 12 nt linker (loop) sequence, were designed (SBI System Biosciences) and annealed (IDT) as described. [score:1]
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2
[+] score: 5
For example, the target site of C. elegans miR-248 matches the E-box, a site known to be bound by several transcription factors of the basic helix-loop-helix family; similarly, the target site of D. melanogaster miR-184* matches the binding site for GATA factors. [score:5]
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