Stem-loop sequence hsa-let-7g

Accession

MI0000433 (change log)

Symbol

HGNC:MIRLET7G

Description

Homo sapiens let-7g stem-loop

Gene family

MIPF0000002; let-7

Literature search

1077 open access papers mention hsa-let-7g
(5919 sentences)

Stem-loop

   a   u        a            ugagg    -a  a    a 
5'  ggc gagguagu guuuguacaguu     gucu  ug uacc c
    ||| |||||||| ||||||||||||     ||||  || ||||  
3'  ccg uuccguca cggacaugucaa     uaga  ac augg c
   a   -        c            -----    gg  -    c 

Deep sequencing

50510152 reads, 1.03e+05 reads per million, 159 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

let-7g-3p cloned in [2] has a 1 nt 3' extension (U), which is incompatible with the genome sequence.

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr3: 52268278-52268361 [-]
• Overlapping transcripts:

  sense

  OTTHUMT00000350199 ; TWF2-001; intron 2

  OTTHUMT00000367018 ; TWF2-005; intron 2

  ENST00000597542 ; TLR9-201; intron 2

  ENST00000305533 ; TWF2-001; intron 2

  ENST00000499914 ; TWF2-005; intron 2

Database links

• EntrezGene:MIRLET7G
• HGNC:MIRLET7G
• RNAcentral:URS00003DBD40_9606
• miRBase Tracker:MI0000433

Mature sequence hsa-let-7g-5p

• Accession: MIMAT0000414
• Previous IDs: hsa-let-7g
• Sequence: 5 - ugagguaguaguuuguacaguu - 26
• Deep sequencing: 50508337 reads, 159 experiments
• Evidence: experimental; cloned [2-3], Illumina [4]
• Database links:
  • RNAcentral:URS00004AFF8D_9606
• Predicted targets:
  • TargetMiner:hsa-let-7g-5p
  • TargetScanVert:hsa-let-7g-5p
  • miRDB:hsa-let-7g-5p
  • microrna.org:hsa-let-7g-5p

Mature sequence hsa-let-7g-3p

• Accession: MIMAT0004584
• Previous IDs: hsa-let-7g*
• Sequence: 62 - cuguacaggccacugccuugc - 82
• Deep sequencing: 1795 reads, 125 experiments
• Evidence: experimental; cloned [2]
• Database links:
  • RNAcentral:URS000029979D_9606
• Predicted targets:
  • TargetMiner:hsa-let-7g-3p
  • TargetScanVert:hsa-let-7g-3p
  • miRDB:hsa-let-7g-3p
  • microrna.org:hsa-let-7g-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).
4. PMID:20158877 "Analysis of deep sequencing microRNA expression profile from human embryonic stem cells derived mesenchymal stem cells reveals possible role of let-7 microRNA family in downstream targeting of hepatic nuclear factor 4 alpha" Koh W, Sheng CT, Tan B, Lee QY, Kuznetsov V, Kiang LS, Tanavde V BMC Genomics. 11 Suppl 1:S6(2010).