miRBase entry: cel-mir-355

Stem-loop cel-mir-355


Accession
MI0000754
Description
Caenorhabditis elegans cel-mir-355 precursor miRNA
Gene family
MIPF0000236; mir-355

Literature search
2 open access papers mention cel-mir-355
(22 sentences)

Sequence

3914 reads, 252 reads per million, 12 experiments
aauaaugucaagaauugaauggUUUGUUUUAGCCUGAGCUAUGagucaucggaauaugcaUAGCUUCUUGCUAAAACAUGCCaaucaaucuuaauugacgaaguuuuuga
......(((((..(((((.((((.(((((((((..((((((((..((....)).....)))))).))..))))))))).)))).))))).....)))))...........

Structure
-----aauaau     ---ga     a    U         CU  -      ---ag  a 
           gucaa     auuga uggU UGUUUUAGC  GA GCUAUG     uc u
           |||||     ||||| |||| |||||||||  || ||||||     ||  
           caguu     uaacu aCCG ACAAAAUCG  CU CGAUac     ag c
aguuuuugaag     aauuc     a    U         UU  U      guaua  g 


Annotation confidence Not enough data
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Comments
This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing [1]. The 5' end of the mature sequence was mapped by PCR, and the 3' end confirmed later [2].

Genome context
chrII: 11833536-11833645 [-]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-355
Name Accession Chromosome Start End Strand Confidence




Database links

Mature cel-miR-355-5p

Accession MIMAT0000697
Description Caenorhabditis elegans cel-miR-355-5p mature miRNA
Sequence 23 - UUUGUUUUAGCCUGAGCUAUG - 43
Evidence experimental
cloned [1-2], PCR [1], Illumina [3]
Database links
Predicted targets

Mature cel-miR-355-3p

Accession MIMAT0031895
Description Caenorhabditis elegans cel-miR-355-3p mature miRNA
Sequence 61 - UAGCUUCUUGCUAAAACAUGCC - 82
Evidence not_experimental

References

  1. PubMed ID: 17174894
    Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans
    "Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP"
    "Cell (2006) 127:1193-1207

  2. PubMed ID: 19460142
    Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development
    Kato M, de Lencastre A, Pincus Z, Slack FJ
    Genome Biol (2009) 10:R54

  3. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322