Stem-loop sequence mmu-mir-214

Accession

MI0000698 (change log)

Symbol

MGI:Mir214

Description

Mus musculus miR-214 stem-loop

Gene family

MIPF0000062; mir-214

Literature search

120 open access papers mention mmu-mir-214
(1829 sentences)

Stem-loop

   ggccu      acaga           u          aca            aacau 
5'      ggcugg     guugucaugug cugccugucu   cuugcugugcag     c
        ||||||     ||||||||||| ||||||||||   ||||||||||||     c
3'      ccgacc     caacaguacac gacggacaga   ggacgacauguc     g
   ----u      -----           u          cac            cacuc 

Deep sequencing

137323 reads, 1.04e+03 reads per million, 87 experiments

Confidence

Annotation confidence: high

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Comments

Mouse mir-214 is predicted [2] based on homology to a reported miR from human (MI0000290) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr1: 162223368-162223477 [+]
• Overlapping transcripts:

  sense

  OTTMUST00000073023 ; Dnm3os-001; exon 1

  ENSMUST00000139662 ; Dnm3os-001; exon 1

  ENSMUST00000178978 ; Dnm3os-201; exon 1

  antisense

  OTTMUST00000073178 ; Dnm3-003; intron 3

  OTTMUST00000073179 ; Dnm3-004; intron 3

  OTTMUST00000073026 ; Dnm3-006; intron 14

  OTTMUST00000073176 ; Dnm3-001; intron 14

  ENSMUST00000160665 ; Dnm3-004; intron 3

  ENSMUST00000159763 ; Dnm3-003; intron 3

  ENSMUST00000070330 ; Dnm3-006; intron 14

  ENSMUST00000086074 ; Dnm3-001; intron 14

Clustered miRNAs

< 10kb from mmu-mir-214

• mmu-mir-199a-2: chr1: 162217814-162217923 [+]
• mmu-mir-214: chr1: 162223368-162223477 [+]

Database links

• EntrezGene:Mirn214
• MGI:Mir214
• RNAcentral:URS0000160683_10090
• miRBase Tracker:MI0000698

Mature sequence mmu-miR-214-5p

• Accession: MIMAT0004664
• Previous IDs: mmu-miR-214*
• Sequence: 30 - ugccugucuacacuugcugugc - 51
• Deep sequencing: 41028 reads, 69 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00004DAA89_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-214-5p
  • miRDB:mmu-miR-214-5p
  • microrna.org:mmu-miR-214-5p

Mature sequence mmu-miR-214-3p

• Accession: MIMAT0000661
• Previous IDs: mmu-miR-214
• Sequence: 71 - acagcaggcacagacaggcagu - 92
• Deep sequencing: 93861 reads, 87 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00002C11C3_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-214-3p
  • miRDB:mmu-miR-214-3p
  • microrna.org:mmu-miR-214-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).