Stem-loop cel-mir-264

Accession

MI0000344

Description

Caenorhabditis elegans cel-mir-264 precursor miRNA

Sequence

uggcggcggucgacauguacauaucaccagucuggcuuuucccguuuccgcgagcaagcGGCGGGUGGUUGUUGUUAUGggauaccggagcca
((((..((((...((((.(((.((((((.(((..(((((((.((....)).))).))))))).))))))...)))))))....))))..))))

Structure

    gg    -cga    u   --u      a   ug    -   c  u 
uggc  cggu    caug aca   aucacc guc  gcuu uuc cg u
||||  ||||    |||| |||   |||||| |||  |||| ||| ||  
accg  gcca    GUAU UGU   UGGUGG CGG  cgaa gag gc u
    ag    uagg    -   UGU      G   --    c   c  c 

Annotation confidence Not enough data

Comments

This precursor sequence was predicted by comparative computational approaches. The excised miRNA sequence is predicted and the precise 5' or 3' end unknown. A PCR amplification protocol confirmed that the strand containing the predicted miR is predominantly expressed.

Genome context

chrX: 2819254-2819346 [+]

Mature cel-miR-264

• Accession: MIMAT0000320
• Description: Caenorhabditis elegans cel-miR-264 mature miRNA
• Sequence: 60 - GGCGGGUGGUUGUUGUUAUG - 79
• Evidence: experimental ; PCR [1]

References

1. PubMed ID: 12769849
   Computational and experimental identification of C. elegans microRNAs
   "Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J"
   "Mol Cell (2003) 11:1253-1263