Stem-loop aae-mir-12

Accession

MI0013462

Description

Aedes aegypti aae-mir-12 precursor miRNA

Literature search

6 open access papers mention aae-mir-12
(83 sentences)

Sequence

21928 reads, 1123 reads per million, 2 experiments

uucggacUGAGUAUUACAUCAGGUACUGGUgucgauauucgaaucaccaacgaaucggguuccuuugauuaacuuacCAGUACUUAUGUUAUGCUCUCUccg
..((((..((((((.((((.(((((((((((((((.((((((.((......)).))))))....)))))......)))))))))))))).))))))..))))

Structure

uu    cU      U    C          ------     ---u      a  ac 
  cgga  GAGUAU ACAU AGGUACUGGU      gucga    auucga uc  c
  ||||  |||||| |||| ||||||||||      |||||    |||||| ||   
  gccU  CUCGUA UGUA UUCAUGACca      uaguu    ugggcu ag  a
--    CU      U    -          uucaau     uccu      a  ca 

Annotation confidence High

Comments

This miRNA was identified independently in Aedes aegypti [1] and in the closely related Aedes albopictus, for which there was no genome sequence [2]. The latter reads were therefore also mapped to the Aedes aegypti genome [2].

Genome context

supercont1.68: 2720396-2720497 [-]

Clustered miRNAs

2 other miRNAs are < 10 kb from aae-mir-12

Mature aae-miR-12-5p

• Accession: MIMAT0014253
• Description: Aedes aegypti aae-miR-12-5p mature miRNA
• Sequence: 8 - UGAGUAUUACAUCAGGUACUGGU - 30
• Evidence: experimental ; 454 [1], Illumina [2]

Mature aae-miR-12-3p

• Accession: MIMAT0014254
• Description: Aedes aegypti aae-miR-12-3p mature miRNA
• Sequence: 78 - CAGUACUUAUGUUAUGCUCUCU - 99
• Evidence: experimental ; Illumina [2]

References

1. PubMed ID: 19961592
   Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs
   Li S, Mead EA, Liang S, Tu Z
   BMC Genomics (2009) 10:581


2. PubMed ID: 20167119
   Identification of microRNAs expressed in two mosquito vectors, Aedes albopictus and Culex quinquefasciatus
   Skalsky RL, Vanlandingham DL, Scholle F, Higgs S, Cullen BR
   BMC Genomics (2010) 11:119