Stem-loop mmu-mir-1a-2

Accession

MI0000652

Symbol

MGI: Mir1a-2

Description

Mus musculus mmu-mir-1a-2 precursor miRNA

Literature search

287 open access papers mention mmu-mir-1a-2
(2998 sentences)

Sequence

20208824 reads, 19830 reads per million, 99 experiments

ucagagcACAUACUUCUUUAUGUACCCAUAugaacauucagugcuaUGGAAUGUAAAGAAGUAUGUAUuuug
.(((((.((((((((((((((((..(((((.(.((.....)).)))))).)))))))))))))))).)))))

Structure

u     c                AC     u a  a 
 cagag ACAUACUUCUUUAUGU  CCAUA g ac u
 ||||| ||||||||||||||||  ||||| | || u
 guuuU UGUAUGAAGAAAUGUA  GGUau c ug c
-     A                -A     - g  a 

Annotation confidence High

Comments

Lagos-Quintana et al. [1] reported the cloning of miR-1b, miR-1c and miR-1d. The mature processed miR sequences are identical apart from the 3' residues (A in mir-1b, C in mir-1c and UU in mir-1d). The 3' residues of both miR-1b and miR-1c conflict with the predicted stem-loop precursor sequence shown here and these sequences are not found in current assemblies of human and mouse genomes. It is suggested that polyA polymerase may add 1-3 nts to the 3' end of the mature transcript (Tom Tuschl, pers. comm.). The common 21 nts of the 3 reported miR sequences have been rationalised here and named miR-1. There are 2 pairs of orthologous putative hairpin precursor structures named mir-1-1 (human MIR:MI0000651, mouse MIR:MI0000139), and mir-1-2 (human MIR:MI0000437, mouse MIR:MI0000652). The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2].

Genome context

chr18: 10785481-10785552 [-]

Clustered miRNAs

2 other miRNAs are < 10 kb from mmu-mir-1a-2

Mature mmu-miR-1a-3p

• Accession: MIMAT0000123
• Description: Mus musculus mmu-miR-1a-3p mature miRNA
• Sequence: 47 - UGGAAUGUAAAGAAGUAUGUAU - 68
• Evidence: experimental ; cloned [1-2], Illumina [3]

Mature mmu-miR-1a-2-5p

• Accession: MIMAT0017047
• Description: Mus musculus mmu-miR-1a-2-5p mature miRNA
• Sequence: 8 - ACAUACUUCUUUAUGUACCCAUA - 30
• Evidence: experimental ; Illumina [3]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 12007417
   Identification of tissue-specific microRNAs from mouse
   "Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T"
   "Curr Biol (2002) 12:735-739


3. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009