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5 publications mentioning csi-MIR164c

Open access articles that are associated with the species Citrus sinensis and mention the gene name MIR164c. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 94
Vector ox-miR164 overexpress csi-miR164, ox-miRCon overexpress a control miRNA, ox-10TS + GFP overexpress a GFP gene carrying target site of miR164 in Cs5g10870, ox-10MTS + GFP overexpress a GFP gene carrying a modified target site. [score:13]
Target site of miR164 in Cs5g10870 and a modified target site (inactivated target site) were inserted into a green fluorescent protein (GFP) gene overexpression vector respectively as shown in Figure  9b. [score:9]
2 were expressed at higher levels in flower than the other tissues; miR5179 was specifically expressed in flower (c) miR3951 and miR164 were expressed at higher levels in fruit than the other tissues; miR477a-3p was specifically expressed in fruit. [score:9]
As a result, csi-miR164 targeted the target region of Cs5g10870 and repressed the expression of GFP obviously (Figure  9e). [score:7]
In this assays, we used Agrobacterium tumefaciens infiltration to co-express csi-miR164, control miRNA, GFP gene carrying target site and GFP gene carrying modified target site pair by pair (Figure  9c). [score:6]
As described above, miRNAs act as regulators of plant development [18, 19]; miR156, miR164 and miR166, in particular, play important roles in regulating leaf development [20– 24]. [score:5]
As showed in Figure  9a, the expression level of csi-miR164 and Cs5g10870 displayed complementary expression pattern. [score:5]
As csi-miR164 was higher expressed in fruit and was more active at the late stages during the orange fruit development, we suggested that csi-miR164 might be involved as a regulator in orange ripening by interacting with a NAC transcription factor. [score:5]
Interestingly, Csi-miR164 was highly expressed in fruit ripening stage, and was validated to target a NAC transcription factor. [score:5]
Csi-miR164 showed significantly higher expression level at the final stage (fruit ripening stage), with a steady increase in expression level during fruit ripening (170–230 DAF). [score:5]
To confirm the interaction between csi-miR164 and Cs5g10870 in orange fruit, we detected the expression level of Cs5g10870 by qRT-PCR at fruit developmental stages. [score:4]
Figure 9 Csi-miR164 targeted Cs5g10870 was verified in vivo. [score:3]
Two targets of csi-miR164 were identified in fruit; the first was annotated as a NAC transcription factor gene which was confirmed by (Figure  7) and the second was annotated as a thymidine diphospho-glucose 4-6-dehydratase gene. [score:3]
As mentioned above, csi-miR164 was highly active during fruit ripening and 5’RACE analysis showed csi-miR164 targeted Cs5g10870, a NAC transcription factor. [score:3]
Furthermore, a transient expression system was used to confirm that csi-miR164 degraded Cs5g10870 in vivo. [score:3]
Csi-miR164 was further validated to functions by target NAC transcription factor. [score:3]
Overexpression vectors of csi-miR164 and a control miRNA were constructed respectively. [score:3]
The results showed that csi-miR164, csi-miR3951, csi-miR477a-3p and csi-miRN31 have remarkably higher expression in fruits compared with other tissues. [score:2]
Therefore, csi-miR164, as a known miRNA, may have new function in orange fruit ripening on posttranscriptional level. [score:1]
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2
[+] score: 64
The adaptive responses of leaf miRNAs to B-deficiency might be associated with several aspects: (a) attenuation of plant growth and development by down -regulating TIR1, ARF and AFB due to up-regulated miR393 and miR160, and by lowering the expression of SAUR-like auxin-responsive protein family targeted by miR3946, thus enhancing plant stress tolerance; (b) improving the expression of NACs due to decreased expression miR159, miR782, miR3946 and miR7539, hence maintaining leaf phenotype and enhancing the stress tolerance; (c) activation of the stress responses and antioxidant system due to decreased expression of miR164, miR6260, miR5929, miR6214, miR3946 and miR3446; (d) decreased expression of MFS resulting from increased expression of miR5037, thus lowering B export from plants. [score:20]
The adaptive responses of miRNAs to B-deficiency might related to several aspects: (a) attenuation of plant growth and development by repressing auxin signaling due to decreased TIR1 level and ARF -mediated gene expression by altering the expression of miR393, miR160 and miR3946; (b) maintaining leaf phenotype and enhancing the stress tolerance by up -regulating NACs targeted by miR159, miR782, miR3946 and miR7539; (c) activation of the stress responses and antioxidant system through down -regulating the expression of miR164, miR6260, miR5929, miR6214, miR3946 and miR3446; (d) decreasing the expression of major facilitator superfamily protein genes targeted by miR5037, thus lowering B export from plants. [score:16]
However, Xu et al. found that miR164 was up-regulated in maize leaves under chronic N limitation, and suggested that miR164 might function in remobilizing the N from old to new leaves to cope with the N-limiting condition via accelerating senescence due to decreased expression of NAC [28]. [score:6]
Therefore, the down-regulation of miR164 in B -deficient leaves might be involved in the B-deficiency tolerance of plants by improving the expression of NAC. [score:6]
Water stress led to decreased expression of miR164 in cassava (Manihot esculenta) leaves, while its target gene MesNAC (No Apical Meristem) was strongly induced [49]. [score:5]
Also, GRAS is the target gene of miR1446 (Table  2), miR170 and miR171 [58], and NAC is the target gene of miR164, miR3953 and miR3946 (Table  2). [score:5]
Leaf miR164 was down-regulated by B-deficiency (Table  2), as previously observed on transient low nitrate-stressed maize leaves [28]. [score:4]
1 ARF17 0.9153** miR164 −2.28320824** orange1.1g030909m AT1G56010.2 NAC domain containing protein 1 0.5939** orange1.1g047710m AT5G53950.1 NAC domain transcriptional regulator superfamily protein 1.4205** orange1.1g017827m AT5G61430.1 NAC domain containing protein 100 1.3247** orange1.1g017636m AT3G08030.1 Protein of unknown function, DUF642 0.5400** miR158 −3.35603222** orange1.1g022993m AT1G69840.1 SPFH/Band 7/PHB domain-containing membrane -associated protein family 1.9490** AT2G03210 Fucosyltransferase 2 1.6482** orange1.1g001709m AT3G07400 Lipase class 3 family protein 0.7819* miR393 1.66802767** orange1.1g010049m AT3G18080.1 B-S glucosidase 44 0.8384** orange1.1g007916m At3g62980 TIR1 0.7489** At4g03190 AFB1 0.8195** orange1.1g008325m At3g26810 AFB2 0.7895** At1g12820 AFB3 1.6782** miR408 −2. [score:2]
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3
[+] score: 56
miRNA Fold change of miRNA Accession Homology Target genes Potential roles Relative change of target genes miR164−2.38730699 [**] orange1.1g030909m AT1G56010.2 NAC domain containing protein 1 Transcription1.4372 [**] orange1.1g047710m AT5G53950.1 NAC (No Apical Meristem) domain transcriptional regulator superfamily protein Transcription0.6610 [**] orange1.1g017827m AT5G61430.1 NAC domain containing protein 100 Transcription2.9265 [**] miR158−6.05735341 [**] orange1.1g001709m AT3G07400 Lipase class 3 family protein Lipid metabolism4.1384 [**] orange1.1g002569m AT5G63020.1 Disease resistance protein (CC-NBS-LRR class) family Disease, virulence and defense3.2507 [**] orange1.1g038105m AT1G12220.1 Disease resistance protein (CC-NBS-LRR class) family Disease, virulence and defense1.6141 [**] orange1.1g041843m AT1G12280.1 LRR and NB-ARC domains-containing disease resistance protein Disease, virulence and defense0.7305 [**] miR8335.7705023 [**] orange1.1g047519m AT1G45616.1 Receptor like protein 6 Disease, virulence and defense0.5503 [**] miR15071.99337925 [**] orange1.1g034576m AT3G14470.1 NB-ARC domain-containing disease resistance protein Disease, virulence and defense0.1031 [**] orange1.1g042037m AT3G14460. [score:24]
The adaptive responses of leaf miRNAs to Mg-deficiency might include following several aspects: (a) inducing stress-related genes by repressing miR164, miR7812, miR5742, miR3946, and miR5158; (b) up -regulating transport-related genes; (c) increasing the expression of genes related to lipid metabolism by inhibiting miR158, miR5256, and miR3946 expression; (d) activating cell wall-related gene expansis 8A by down -regulating miR779; and (e) down -regulating the expression of genes involved in the maintenance of S, K and Cu by up -regulating miR395 and miR6426. [score:13]
Therefore, Mg-deficiency -induced down-regulation of leaf miR164 might play a role in the tolerance of plants to Mg-deficiency by enhancing the expression of NAC. [score:6]
Xu et al. (2011) observed that the expression level of miR164 in maize leaves increased in response to chronic N limitation, concluding that miR164 might function in remobilizing the N from old to new leaves via boost senescence due to decreased expression of NAC under N limitation. [score:5]
Involvement of miR164- and miR167 -mediated target gene expressions in responses to water deficit in cassava. [score:5]
The expression level of miR164 was decreased in Mg -deficient leaves (Table 2), as found for transient low nitrate-stressed maize leaves (Xu et al., 2011) and water stressed cassava (Manihot esculenta) leaves (Phookaew et al., 2014). [score:3]
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4
[+] score: 5
Many putative targets are transcription factors and homologs of known miRNA target genes in other plant species, such as SBP for miR156, NAC for miR164, bZIP for miR166, AP2 for miR172 and F-box for miR394. [score:5]
[1 to 20 of 1 sentences]
5
[+] score: 3
Other miRNAs from this paper: csi-MIR164a, csi-MIR164b, csi-MIR164d
With the help of next generation sequencing, a microRNA, Csi-miR164, and its function in fruit ripening stage was identified and was validated to target a NAC transcription factor [25]. [score:3]
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