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3 publications mentioning bdo-mir-8

Open access articles that are associated with the species Bactrocera dorsalis and mention the gene name mir-8. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 95
In the case of miR-8-3p, its tissue expression pattern suggests that it regulates mitoferrin expression in thorax and head. [score:6]
Mitoferrin is most abundantly expressed in testes and targeted by miR-8-3p. [score:5]
This is a very convenient method to manipulate gene expression, allowing us to explore the role of miR-8-3p in spermatogenesis and its applicability as a target for SIT without the need to generate transgenic animals. [score:5]
This would argue that mitoferrin is the main target of miR-8-3p and that any other targets affected by increased levels of miR-8-3p play a minor role in spermatogenesis. [score:5]
miR-8-3p was expressed most abundantly in heads, testes and thoraxes and least expressed in whole fly, guts and malpighian tubule (Fig. 1D). [score:5]
However, their analysis did not identify bmfrn as a target of bdo-mir-8. Indeed, also according to our binding-energy analysis (Fig. 1B), mir-8-3p was not the top-rating candidate, but turned out to be the only of the five miRNA candidates that showed a significant interaction with the 3′UTR of bmfrn mRNA (Fig. 3C), underscoring the importance of experimental validation of miRNAs and their targets. [score:5]
Specifically, we show that miR-8-3p regulates mitoferrin (bmfrn) gene expression in the testes of B. dorsalis and is important for spermatogenesis. [score:4]
Dietary delivery of dsRNA, miR-8-3p mimics/antagomiR successfully alters bmfrn expression in testes and reduces reproductive capacity of male flies. [score:3]
Comparison of the relative abundance of miR-8-3p and bmfrn mRNA of control treatments (NC) between days 1 to 12 indicates that they are not differentially expressed over time (Fig. 2A,B). [score:3]
Dietary delivery of miR-8-3p mimics/antagomiRs and bmfrn dsRNA alter bmfrn expression in testes. [score:3]
To silence the expression of miR-8-3p, an antagomiR (antagomiRmiR-8-3p) consisting of the reverse complement of miR-8-3p and for negative control a scrambled RNA (N. C antagomiR) were used 22 33. [score:3]
With the confirmation that dietary delivery of miR-8-3p and bmfrndsRNA successfully alter bmfrn expression in testis, we set out to functionally analyze the consequences of the above treatments on male reproductive capacity (relative number of successfully fertilized eggs), sperm number and sperm viability. [score:3]
Finally, to characterize miR-8-3p expression in more detail, we obtained its tissue expression profile. [score:3]
Increasing or decreasing miR-8-3p levels in testes, as well as reduction of bmfrn expression in the testis result in reduced reproductive capabilities due to reduced sperm production and viability. [score:3]
The relative abundance of miR-8-3p was increased approximately two fold and significantly on days 3to 12of miR-8-3p mimic diet (Fig. 2A), resulting in a strong reduction (approximately 75%)of bmfrn expression in the same time frame (Fig. 2B). [score:3]
In the case of miR-8-3p it cannot be excluded that also other genes than mitoferrin are targeted. [score:3]
However, reduction of mitoferrin expression in the testes by both, miR-8-3p mimic or bmfrn RNAi treatment to similar levels (20% of control respectively, day 7) led to similar reductions in spermatozoa viability and male reproductive capacity (sperm viability: 23% for miR-8-3p mimic and 30% for bmfrn RNAi; male reproductive capacity: 22% for miR-8-3p mimic and 22% for bmfrn RNAi). [score:3]
Treatment with the antagomiR, on the other hand, reduced the relative abundance of miR-8-3p on days 1–12 to about 30–12% of the control treatment (Fig. 2A) and increased the expression of bmfrn mRNA mildly but significantly on day 1 and about 2 fold on days 3–12 (Fig. 2B). [score:3]
We observed that dietary delivery of miR-8-3p or RNAi resulted in reduction of mitoferrin expression in testes and reduction in sperm viability, total number of mature sperms in seminal vesicles and reproductive capacity of male flies. [score:3]
Interestingly and unexpectedly, we observed almost identical effects on male reproductive capacity, sperm viability and sperm counts, when miR-8-3p was inhibited by an antagomiR. [score:3]
Dietary delivery of dsRNA, miR-8-3p mimics/antagomiR successfully alters bmfrn expression in testes and reduces reproductive capacity of male fliesPreviously it was shown that RNAi and miRNA as well as antagomiRs can be successfully delivered to moth larvae and oriental fruit flies via feeding 22 23. [score:3]
How to cite this article: Tariq, K. et al. miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis. [score:2]
miR-8-3p interacts with the mitoferrin 3′UTR and represses mitoferrin mRNAIn our previous work we have obtained miRNA profiles at different developmental stages during spermatogenesis of the oriental fruit fly 14. [score:2]
“a” above the bars indicates significant differences in miR-8-3p expression compared with whole fly homogenate. [score:2]
Different concentrations of miRNA mimic or antagomiR have previously been tested and 100 nM of antagomiR miR-8-3p and 500 nM of miRNA mimic were found to be the most effective 22 34. [score:1]
N = 3. Relative abundances of miR-8-3p (A) and bmfrn (B,C) were determined by qRT-PCR of cDNA made from total RNA isolated from testes 1, 3, 7, and 12 days after the indicated dietary treatments of adult flies. [score:1]
Therefore, we would attribute the decreased sperm viability of miR-8-3p and bmfrn RNAi treated B. dorsalis mainly to insufficient heme and/or Fe/S cluster synthesis in spermatids as proposed for Drosophila mitoferrin loss of function mutants 19. [score:1]
However, our results clearly show that depleting or increasing miR-8-3p or RNAi of mitoferrin in B. dorsalis all lead to significantly reduced sperm viability and reduced male reproductive capacity. [score:1]
This suggests that miR-8-3p is the only miRNA, of the five miRNAs tested, that interacts with the 3′ UTR of mitoferrin mRNA and could be an interesting candidate to develop genetic SIT to control B. dorsalis. [score:1]
From our results it is not possible to identify the specific cause of reduced sperm viability and whether the defects in spermatogenesis or sperm function leading to reduced sperm viability and reproductive capacity are the same in flies treated with miR-8-3p mimic/antagomire or bmfrn dsRNA. [score:1]
However, in flies treated with miR-8-3p or the antagomir we did not notice any obvious alterations in morphology or behavior connected to these tissues. [score:1]
To increase the abundance of miR-8-3p, a miR-8-3p mimic and a NC mimic (unrelated mimic) were used. [score:1]
These results indicate, that the main reason for the reduced reproductive capacity of miR-8-3p mimic/antagomiR or bmfrn dsRNA treated male flies is the result of reduced sperm viability and not as much in the reduction in sperm number. [score:1]
miR-8-3p interacts with the mitoferrin 3′UTR and represses mitoferrin mRNA. [score:1]
The detrimental effect that miR-8-3p antagomiR has on sperm viability could have several reasons. [score:1]
Recently, Calla and Geib reported that bdo-miR-8 is an miRNA conserved between B. dorsalis and Drosophila 24. [score:1]
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[+] score: 13
The results showed that miR-6-3p, miR-989-3p, miR-994-5p, miR-308-3p were significantly more highly expressed in the ovaries, miR-8-3p, miR-1-3p, miR-12-5p, miR-274-5p was preferentially expressed in the testes, while miR-995-3p and miR-276a co-expressed in the ovary and testes (Fig 5). [score:7]
Of the sex-biased miRNAs, some are pairs derived from the same precursor (like miR-5-5p and miR-5-3p; miR-8-5p and miR-8-3p), and most of the miRNAs prefer to cluster in the genome and express in a consistent sex-biased pattern (Table 2). [score:3]
Red reflects miR-8-5p and yellow reflects miR-8-3p. [score:1]
miR-5-3p/miR-5-5p, miR-4-3p/miR-6-3p/miR-309-3p, miR-318-3p/miR-994-5p from female-biased miRNAs and miR-8-5p/miR-8-3p, miR-3477-5p/miR-12-5p, miR-252-5p/miR-1000-5p from male-biased miRNAs are clustered in B. dorsalis genome, suggesting that those clustered miRNA in B. dorsalis is transcribed in multicistronic primary-miRNA. [score:1]
0159591.g002 Fig 2 (A) The predicted hairpin structure of the miR-8 hairpin, colored as miRNA and miRNA*. [score:1]
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[+] score: 2
Other miRNAs from this paper: dme-mir-8
Tariq, K., Metzendorf, C., Peng, W., Sohail, S. & Zhang, H. miR-8-3p regulates mitoferrin in the testes of Bactrocera dorsalis to ensure normal spermatogenesis. [score:2]
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