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3 publications mentioning chi-mir-204

Open access articles that are associated with the species Capra hircus and mention the gene name mir-204. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 38
These observations highlighted two miRNA, miR-204-5p and miR-223-3p, whose expression was the most markedly affected by food deprivation and which may target several DEG, suggesting important roles for both miRNA in the nutritional regulation of gene expression in the mammary gland. [score:8]
Of the 11 nutriregulated miRNA that could target DEG, let-7c-5p, miR-223-3p and miR-204-5p may target seven, five and four DEG, respectively (Table 4). [score:6]
Seven miRNA were chosen for RT-qPCR validation among known miRNA which expression was affected by the food deprivation and based on their level of expression; miR-99a-5p (TaqMan [®] ID 006254_mat, Applied Biosystems), miR-126-3p (TaqMan [®] ID 008451_mat), miR-140-3p (TaqMan [®] ID 471823_mat), miR-222-3p (TaqMan [®] ID 000525), miR-223-3p (TaqMan [®] ID 002295), miR-204-5p (TaqMan [®] ID 000508), miR-409-3p (TaqMan [®] ID 002332). [score:5]
In addition, seven known (miR-99a-3p, miR-188-5p, miR-196a-5p, miR-204-5p, miR-222-3p, miR-223-3p and miR-494-3p) and six predicted miRNA (chr3_4386-3p, chr3_4386-5p, chr12_17655-3p, chr19_26739-5p, chr23_30758-5p and chr27_34538-5p) were expressed at moderate levels with normalized read counts of between 100 and 1,000, and three known (miR-223-5p, miR-541-5p and miR-671-5p) and five predicted (chr3_3319-5p, chr4_6064-5p, chr9_13534-5p, chr12_18027-5p and chr23_30758-5p) were weakly expressed with normalized read counts below 100. [score:5]
In addition, miR-204-5p potentially targets ELF5 (E74-like factor 5) which belongs to the JAK-STAT signalling pathway and is essential for the expression of milk-related genes, particularly those coding for major milk proteins [58, 59]. [score:5]
Probably due to the difference of sensibility and normalization, only the expression of miR-204-5p was found significantly affected when analysed using RT-qPCR. [score:3]
Consequently, the deregulation of miR-204-5p and miR-223-3p by food deprivation may have a significant impact in the mammary gland through the regulation of DEG. [score:3]
Notably, miR-204-5p and miR-223-3p are nutriregulated miRNA with the highest fold change in high throughput sequencing. [score:2]
Elsewhere, miR-204-5p may also target LPIN2 (LiPIN 2), recently characterized in the bovine mammary gland [49] and involved in triacylglycerol accumulation [51]. [score:1]
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2
[+] score: 24
Other miRNAs from this paper: chi-mir-154a, chi-mir-154b, chi-mir-211
KITLG expression is suppressed by chi-miR-204-5p in granulosa cells. [score:5]
As shown in Fig. 2B, chi-miR-204-5p and chi-miR-211 suppressed luciferase expression in the presence of allele 1457A compared with NC and allele 1457C (P < 0.05). [score:4]
In conclusion, the SNP c. 1457A > C in the caprine KITLG gene is a functional SNP in chi-miR-204-5p and chi-miR-211 target sites. [score:3]
Therefore, the SNPs c. 1389C > T, c. 1457A > C and c. 1520G > A may account for the different litter sizes of GD goats because chi-miR-204-5p and chi-miR-211 could change the expression level of the KITLG gene and reduce GC proliferation. [score:3]
In the present study, the c. 1457A > C mutation occurred in the seed region of chi-miR-204-5p binding sites and increased the protein levels of KITLG because the base C reduced the binding affinity of chi-miR-204-5p and the 3′-UTR of the KITLG gene. [score:2]
Note: The 16 bases inthe 5′region sequence of chi-miR-204-5p are the same as that of chi-miR-211. [score:1]
In consideration that the 16 bases in the 5′ region sequences of chi-miR-204-5p and chi-miR-211 are the same, chi-miR-204-5p was used in Western blot analysis. [score:1]
Thus, chi-miR-204-5p was used in Westernblot analysis. [score:1]
Effect of chi-miR-204-5p on granulosa cell viability. [score:1]
GCs of different genotypes were transfected with chi-miR-204-5p mimics to determine whether chi-miR-204-5p affects the protein level of KITLG. [score:1]
After 24 h, the cells were treated with chi-miR-204-5p mimic for 36 h. At the end of culture, 50 μL of MTT (Amresco) was added to each well, including controls. [score:1]
The mimics of miRNAs (chi-miR-211 and chi-miR-204-5p) and their negative controls (GenePharma, Shanghai, China) were co -transfected with the reporter plasmids to a final concentration of 20 nmol/μL. [score:1]
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3
[+] score: 6
The top 10 most significantly differentially expressed miRNAs were miR-21, miR-199b, miR-199c, miR-127, miR-200a, miR-379, miR-200b, miR-204, miR-411a, and miR-493. [score:3]
The top 10 most significantly differentially expressed miRNAs (miR-21, miR-199b, miR-199c, miR-127, miR-200a, miR-379, miR-200b, miR-204, miR-411a, miR-493) were analysed. [score:3]
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