2 publications mentioning bdi-MIR166j

Open access articles that are associated with the species Brachypodium distachyon and mention the gene name MIR166j. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

To address whether the differentially expressed miR166 family members also differentially regulate their target genes, we examined the cleavage events in a target gene encoding a homeobox domain-leucine zipper (HD-ZIP) transcription factor.

This implies that miR166 members may regulate HD-ZIP gene expression in a tissue-preferential manner.

The expression pattern of miR166j was not distinguishable due to sequence similarity with miR166h.

As shown in Figure  9a, the target gene has two major PARE sequences corresponding to cleavage sites by miR166a-eg, miR166h, miR166j and miR166f.

Interestingly, the three miR166 sequences showed differential expression patterns in diverse tissues (Figure  9a).

Given the clustered structure of MIR166h and MIR166j (Figure  3c), it is likely that miR166j expression is similar to miR166h.

However, the panicle library showed only one prominent cleavage site by miR166a-eg, miR166h and miR166j, while miR166f -mediated target cleavage was detected at only a basal level.

We observed that two miRNA families, miR166 and miR156/529, have members with both sequence variation and differential regulation patterns.

Cleavage guided by Bdi-miR166 family members in an HD-ZIP III mRNA at sites two nucleotides apart was also validated with PARE (Figure  9a).

In root tissue, two different PARE sequences were mapped to the cleavage sites of both the root-preferential miR166f and the remaining miR166 family members.

miR166 was used as a control.

In Brachypodium, ten MIR genes encode miR166.

While six MIR166 genes generate identical guide sequences, MIR166h, MIR166j and MIR166f give rise to miRNA variants with slight sequence variations.

Since miR166f has a 5′-end two-nucleotide offset relative to the other miR166 family members, the miR166f -mediated cleavage site is also shifted relative to the cleavage site mediated by other family members.

Examples of alternative RNA folding in precursors of Bdi-miR9483 (a), Bdi-miR2118 (b) and Bdi-miR166 (c).

2

Some of these miRNAs including miR156, miR160, miR166 and mi171 are deeply conserved, even in lower plants such as Physcomitrella patens [45].

For example, seven families (miR156, miR160, miR164, miR166, miR167, miR171 and miR396) had similar number of members in Brachypodium and Arabidopsis, but their sizes were much larger in rice and Populus (Table 4).

Some miRNAs, including miR166, miR319, miR393, and miR396, respond to cold stress in Arabidopsis [16, 17], but in our experiment no obvious change was found for these miRNAs after the cold treatment.

For example, the number of family members for miR156, miR160, miR164, miR166, miR167, miR171 and miR396 in Brachypodium was similar to that in Arabidopsis, but much higher in rice and Populus (Table 4).

MiR164, miR166 and miR172 were represented by two variants and miR169 was represented by four variants in the library (Table 2).