7 publications mentioning ppe-MIR169l

Open access articles that are associated with the species Prunus persica and mention the gene name MIR169l. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Deep sequencing results showed that the expression of miR169 was down-regulated in leaf tissue while qPCR experiment revealed that its expression was up-regulated in leaf tissue after treatment.

The expression of miR169 was induced in leaf but inhibited in root tissues after drought treatment, whereas the expression level of miR171 was induced in root but inhibited in root tissues under drought.

Up-regulation of any miRNA expression levels was considered a positive value while negative values indicate down-regulation For miR156, miR164, miR166, miR168, miR169, miR171 and miR395, the miRNA stem-loop reverse transcription reaction was performed in a volume of 10 µL containing 2, 20, and 200 ng of total RNA samples of leaf and root samples (1 µL), 0.5 µL 10 mM dNTP mix, 1 µL stem-loop RT primer (1 µM) and 7.5 µL nuclease free water.

Two members, expression level of miR169a and miR169c were substantially down-regulated in Arabidopsis via ABA -dependent way [52] and also showed that level of miR169 was decreased under drought stress in M. truncatula by using high-throughput sequencing and qRT-PCR methods [66].

The expression level of miR159, miR169, miR393, miR397, miR398 and miR393 were only decreased in root under drought stress while the miR395 were only down-regulated in leaf in response to drought (Figure 4).

The target transcripts of Ppe-mir156, Ppe-mir166, Ppe-mir168, Ppe-mir169, Ppe-mir171, and Ppe-mir395 were obtained using psRNATarget (user-submitted transcripts and miRNA option) and BlastN algoritms.

These results show good concordance with our findings that miR169 was down-regulated in peach after treatment.

qRT-PCR was also used for detection and quantification of predicted targets of six drougt-responsive miRNAs (miR156, miR164, miR166, miR169, miR171 and miR395).

In addition, expression level of miR169 increased in two cowpea genotypes [69].

The miR169 targets a gene family encoding the alpha subunit of CCAAT -binding NFY transcription factors (NFYA) requiring for adoptation to drought stress [8].

It was revealed that the miR169 family members were associated with drought response and high salt stress [52], [55].

Among them, drought responsive miRNAs (miR156, miR164, miR166, miR168, miR169, miR171, and miR395) were detected and their expression levels were measured by qRT-PCR.

2

miR169 can target NFYA to control dehydration responses [47].

Our experiment also identified a high confidence cleavage site for miR169 in peach NFYA.

3

With the exception of miR169 and miR530, the expression levels of the other conserved miRNAs were found to be very low or even almost undetectable in the leaves compared with the levels in the fruit and phloem tissues.

For example, miR156, miR160, miR162, miR164, miR166, miR167, miR169, miR172, miR396, and miR397 are perfectly identical to the corresponding miRNAs in three other plant species (Arabidopsis thaliana, Populus trichocarpa, and Oryza sativa) (S3 Table).

4

The tissue-specific expression patterns were presented for the conserved miRNAs, miR160, miR167, miR169, miR319, miR390 and miR396, and the less-conserved miRNAs, miR828, miR858 and miR2118 (Figure 2b).

5

These researchers characterized different miRNAs whose targets were various TF genes [NFYA (miR169) and DRE (miR169)] involved in plant responses to drought.

6

miRNA families such as miR156, miR169, miR172, miR395, and miR5021 have the largest number of members with the latter having 18 members.

7

In addition, 11 miRNA families (miR162, miR164, miR167, miR168, miR169, miR172, miR393, miR394, miR397, miR399 and miR827) shared a high conservation in both dicotyledons and monocotyledons.