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7 publications mentioning stu-MIR164

Open access articles that are associated with the species Solanum tuberosum and mention the gene name MIR164. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 71
The mutant of Arabidopsis thaliana miR164a and miR164b expressed lower miR164 and higher NAC1 mRNA levels lead to more lateral root occurred, while induced expression in wild type plants expressed miR164 can lead to NAC1 mRNA levels suppressed, and then reduce the occurrence of lateral root (Tang et al., 2012). [score:9]
Furthermore, expression of StNAC262 in transgenic miR164 plants was downregulated under PEG stress (Figure 4A), while the drought stress increased significantly the expression of StNAC262 in transgenic StNAC262 plants compared with those in WT plants (Figure 4B). [score:7]
qRT-PCR analysis demonstrated that the expression of StNAC262 gene in the transgenic potato lines T1 and T2, which overexpress miR164, was downregulated (0.58- to 0.25-fold) compared with the control, particularly in the roots of T1 (0.25-fold) (Figure 3). [score:7]
We concluded that decreased expression of Stu-miR164 drives overexpression of the potato NAC gene and can help plant response to osmotic stress. [score:5]
In Brassica and rice seedlings, miR164 was shown to target a NAC TF, whose expression was negatively correlated with miR164 under stress conditions including drought, salinity, and high-temperature (Bhardwaj et al., 2014; Fang et al., 2014). [score:5]
The overexpressions of StNAC262 in “Kexin 3” result in a high number of lateral roots although shorter than the control, while overexpressions of miR164 in “Gannongshu 2” result in a low number of lateral roots although the same length as the control (Figure 4). [score:5]
The transgenic potato plants (T1 and T2 overexpressing miR164, and T3 and T4 overexpressing StNAC262) and the non-transgenic potato plants were propagated in vitro by sub-culturing single-node cuttings on MS media containing 3% sucrose and cultured for 3 weeks. [score:5]
Conserved miR164 -targeted NAC genes negatively regulate drought resistance in rice. [score:4]
The target genes of Arabidopsis thaliana miR164 family are five genes which coding NAM/ATAF/CUC (NAC) structure domain protein mRNA (Laufs et al., 2004). [score:3]
Relative expressional levels of StNAC262 in response to osmotic stress treatment for 0, 4, 8, 16, and 32 h. (A) qRT-PCR analysis of potato “Gannongshu 2. ” CK1: non-transgenic potato “Gannongshu 2” as negative control; T1–T2: the transgenic potato plant transformed with the vector pCPB121-miR164. [score:3]
Many conserved miRNAs of plant, such as miR156, miR159, or miR164, have been shown to target stress-related TFs including MYB and NAC family members (Burcu et al., 2016). [score:3]
Plants expressing a miR164-resistant CUC2 gene reveal the importance of post-meristematic maintenance of phyllotaxy in Arabidopsis. [score:3]
Many conserved miRNAs, such as miR156, miR159, or miR164, have proved to target stress -associated MYB and NAC family members TFs across monocots and dicots (Burcu et al., 2016). [score:3]
Arabidopsis thaliana miR164 plays an important role in the process in regulating the meristem border size, differentiation of embryo and formation of floral organ (Ohnishi et al., 2005). [score:2]
CK1: non-transgenic potato “Gannongshu 2” as negative control; T1–T2: the transgenic potato plant transformed with the vector pCPB121-miR164; CK2: non-transgenic potato “Kexin 3” as negative control; T3–T4: the transgenic potato plant transformed with the vector pCPB-NAC262. [score:1]
The slices soaked into the Agrobacterium containing the vectors pCPB121-miR164 and pCPB-NAC262, after 7 min infection, the tissue slices were dried with filter paper placed into MS solid media at 28°C in the dark for 48 h. After co-cultivation the tissue slices were placed in differentiation media (MS + 1 mg/L IAA + 0.2 mg/L GA [3] + 0.5 mg/L 6-BA + 2 mg/L ZT + 50 mg/L Kan + 500 mg/L carbenicillin), at 25°C for 2500 lx light to culture, and transferred every weeks to replace media. [score:1]
The engineered vectors, named pCPB-NAC262 and pCPB121-miR164, were, respectively, transformed into Escherichia coli DH5α, which was identified by double enzyme digested for further using. [score:1]
miR164 identified from drought-stress roots of wheat can be used as candidate to develop tolerant variety to explore and exploit the drought response for future studies (Akpinar et al., 2015). [score:1]
We choose the rooting potatoes for further study and named as T1/T2 from “Gannongshu 2” transformed using the vector pCPB121-miR164, and T3/T4 from “Kexin 3” transformed using the vectors pCPB-NAC262. [score:1]
CK1: non-transgenic potato “Gannongshu 2” as negative control; T1–T2: the transgenic potato transformed with the vector pCPB121-miR164; CK2: non-transgenic potato “Kexin 3” as negative control; T3–T4: the transgenic potato plant transformed with the vector pCPB-NAC262. [score:1]
We successfully generated transgenic “Kexin 3” plants with the vector pCPB-NAC262, and transgenic “Gannongshu 2” plants with the vector pCPB121-miR164. [score:1]
[1 to 20 of 21 sentences]
[+] score: 43
The NIb (nuclear inclusion protein b) gene was targeted by 26 miRNAs, and four of the miRNAs (miR162b-3p, miR164-3p, miR7992-3p, and miR8000) were found to be more important because they targeted the gene at 10 different positions. [score:5]
It is imperative to discuss that only one miRNA (miR162b-3p) targeted the NIb gene at four different positions, while the other three miRNAs(miR164-3p, miR7992-3p, and miR8000) targeted the gene at two loci (Figure 3). [score:5]
miR162b-3p potentially targeted the gene at four multiple positions, and the other three miRNAs (miR164-3p, miR7992-3p, and miR8000) targeted the gene at 6 possible sites. [score:5]
Different families of miRNAs of Solanum tuberosum have considerable potential to target potato Virus-Y. miRNA families, such as miR166c-3p, miR482e-5p, miR5303a, miR5303d, miR8004, miR8032b-5p, miR8032c, miR8032e-5p, miR162b-3p, miR164-3p, miR160a-5p, miR8011a-5p, miR8018, and miR482e-5p, were found to have more potential to target PVY at multiple loci. [score:5]
miRNAs targeting HC-Pro (helper component proteinase) geneSimilarly, miRNAs, such as miR164-3p, miR166c-3p, miR171a-3p, and miR171d-3p, targeted the HC-pro gene at multiple loci, while the gene was attacked by 19 miRNAs (Figure 5). [score:5]
miR164-3p targeted the HC-Pro gene at 3 different loci, and each miRNA(miR166c-3p, miR171a-3p, miR171d-3p) targeted HC-Pro at 2 different positions (Figure 4). [score:5]
In this study, we found 19 miRNAs (miR164-3p, miR166c-3p, miR171a-3p, miR171d-3p, miR160a-5p, miR395a, miR395b, miR395i, miR395j, miR399h, miR399l-5p, miR408a-3p, miR482d-5p, miR8000, miR8004, miR8006-3p, miR8032b-3p, miR8032d-3p, and miR8032f-3p) that potentially targeted the HC-Pro (helper component proteinase) gene. [score:3]
The NIb gene was targeted by 20 different miRNAs (miR156d-3p, miR160a-5p, miR162b-3p, miR164-3p, miR166c-3p, miR166d-5p, miR167b-3p, miR167d-3p, miR169b-3p, miR169c-3p, miR5303a, miR5303d, miR7991a, miR7992-3p, miR7992-5p, miR7997b, miR8000, miR8006-5p, miR8011a-5p, and miR8018). [score:3]
Similarly, miRNAs, such as miR164-3p, miR166c-3p, miR171a-3p, and miR171d-3p, targeted the HC-pro gene at multiple loci, while the gene was attacked by 19 miRNAs (Figure 5). [score:3]
The other three miRNAs (miR6149-3p, miR8006-3p, and miR8032b-3p) targeted the CP gene at two different positions (Figure 6), and remaining eight miRNAs (miR156d-3p, miR164-3p, miR167b-3p, miR399h, miR399l-5p, miR7996c, miR8004, and miR8032d-3p) attached at a single position. [score:3]
Among the 19 miRNAs, 4 (miR164-3p, miR166c-3p, miR171a-3p, and miR171d-3p) were found to be more critical because of their potential interaction at nine loci of the HC-Pro gene. [score:1]
[1 to 20 of 11 sentences]
[+] score: 43
Among conserved miRNA targets, most of them were found to be transcripts coding for transcription factors (Additional file 5), such as Squamosa promoter -binding protein (regulated by miR156), GRAS family transcription factors (targeted by miR171), GAMYB-like2 (targeted by miR159), APETALA2 (target of miR172), NAC domain containing protein (targeted by miR164), Auxin response factors (regulated by miR160), PHAVOLUTA-like HD-ZIPIII protein (target of miR166) and nuclear transcription factors - YA4, YA5, YA6 (targeted by miR169). [score:17]
In addition to transcription factors, other targets included mRNA coding for F-box family protein (miR394), disease resistance protein and fiber expressed protein (miR159), laccase (miR397), salt tolerance protein (miR157), UDP-glucoronate decarboxylase 2 (miR164), DNA binding protein (miR166 and miR396), NL25 disease resistance protein (miR482), protein phosphatase and kinase (miR390), AGO1-1 (miR168), galactose oxidase (miR6149) and proteins with unknown functions (Additional file 5). [score:9]
Targets were predicted for identified conserved and potato-specific miRNAs, and predicted targets of four conserved miRNAs, miR160, miR164, miR172 and miR171, which are ARF16 (Auxin Response Factor 16), NAM (NO APICAL MERISTEM), RAP1 (Relative to APETALA2 1) and HAM (HAIRY MERISTEM) respectively, were experimentally validated using 5′ RLM-RACE (RNA ligase mediated rapid amplification of cDNA ends). [score:5]
It has been also reported that miR164 is a key regulator in diverse developmental processes including floral, embryonic, vegetative organs and lateral root development [48, 49]. [score:4]
The predicted targets of miR160, miR164, miR172 and miR171 were ARF16, NAM, RAP1 and HAM, respectively. [score:3]
Among conserved miRNAs, miR164 was predicted to target mRNA encoding for NAC domain containing proteins. [score:3]
Our RACE results confirmed that transcripts of AFR16, NAM, RAP1 and HAM were cleaved in vivo by miR160, miR164, miR172 and miR171, respectively. [score:1]
Similar variations in read abundance were also observed among members of the miRNA families such as miR171 (0.1–1269 TPM), miR164 (0.2–6089 TPM), miR399 (0.5–232 TPM) and miR166 (23888–82963 TPM). [score:1]
[1 to 20 of 8 sentences]
[+] score: 9
We also inspected sRNA -mediated responses which resembled responses in mutualistic symbioses in tolerant plants of Désirée and found that miR482e was also upregulated in NahG plants, while regulation of miR164, miR167, miR169, miR171, miR319, miR390, and miR393, was diminished (Dataset S1). [score:5]
Désirée, such as miR164, miR167, miR169, miR171, miR319, miR390, and miR393 have also been reported to regulate nodulation and arbuscular mycorrhizal symbiosis in different legume species (Dataset S1; Lelandais-Brière et al., 2009; Devers et al., 2011; Mao et al., 2013; Yan et al., 2015). [score:2]
miR393 and miR164 influence indeterminate but not determinate nodule development. [score:2]
[1 to 20 of 3 sentences]
[+] score: 9
Stu-miR164-5p which targeted an NAC TF (PGSC0003DMG400022134), was down-regulated at 24hpi and 60hpi. [score:6]
The expression analysis showed that most of these DEMs decreased at 60hpi, including stu-miR164-5p, stu-miR397-5p and stu-miR408a-3p (Fig.   7B,C and Table  S7). [score:3]
[1 to 20 of 2 sentences]
[+] score: 3
Similarly, Zhang et al. [20] predicted numerous similar lncRNAs in O. sativa, and two of them were proven to bind to miR160 and miR164, individually, thus playing a role in the regulation of reproductive development. [score:3]
[1 to 20 of 1 sentences]
[+] score: 2
Products of miR159, MYB33, and/or MYB101 genes that act downstream of CBP80 have been shown to be involved in the ABA- mediated regulation of potato responses to drought and similar studies have also identified and characterized microRNA families for drought stress response and their putative target genes including miR171 (stu-miRNA171a, b, and c), miR159, miR164, miR166, miR390, miR395, miR397, miR398, miR408, and miR482 (Hwang et al., 2011a, b; Pieczynski et al., 2013; Zhang et al., 2013). [score:2]
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