5 publications mentioning bdi-MIR156f

Open access articles that are associated with the species Brachypodium distachyon and mention the gene name MIR156f. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

In our data, Bdi-miR156 and Bdi-miR529 also showed a similar differential regulation of their target gene, Bdi-SPL14L, when they were expressed (Figure  9b).

Bdi-miR156 was highly expressed in young shoot tissues, and downregulated in mature leaf tissues (Figure  9b).

Since Bdi-miR529 and Bdi-miR156 share several target genes and may differ for others, their over -expression should be significant and, depending on the outcome, may suggest strategies to improve food or bioenergy crops.

In addition, Bdi-miR156 expression in panicle tissue was much lower than that in the other tissues, whereas Bdi-miR529 was preferentially expressed in panicle tissue.

Increased expression of OsSPL14 due to sequence alteration of the miR156 target site leads to less tillers and more panicle branching, resulting in increased grain yields in rice [68, 69].

It has been reported that the regulation of OsSPL14 expression by Osa-miR156 and Osa-miR529 may play a crucial role in both tillering and panicle-branching in rice [34, 68, 69].

To explore this regulation in Brachypodium, we first examined the expression pattern of Bdi-miR156 and Bdi-miR529.

Known and potential outcomes of modulating miR156/529 expression.

Sequence variations and differential expression patterns between miR156 and miR529 could cause their distinct function in SPL gene repression.

In contrast to miR156, the effects of over -expressing miR529 have not been examined yet for transgenic plants.

However, over -expression of miR156 can also result in other defects like dwarfism and male sterility, likely because miR156 represses many SPL genes.

Recently, it has been shown that over -expression of miR156 in rice, maize, switchgrass and Brachypodium caused morphological alterations including an increased number of branches, resulting in a favorable phenotype for improved biomass production [70, 82- 84].

This result implies that cleavage of BdiSPL14L during vegetative growth is predominantly miR156 -guided, whereas miR529 and miR156 regulate the same gene during reproductive development.

We observed that two miRNA families, miR166 and miR156/529, have members with both sequence variation and differential regulation patterns.

In leaf tissue of three-week-old plants, a prominent PARE sequence was mapped to a cleavage site by miR156, while the PARE sequence from the site of miR529 -mediated cleavage had a very low abundance.

In contrast, the PARE sequence corresponding to the site of cleavage mediated by panicle-preferential miR529 was more abundant than that of miR156 in the panicle library.

The distinct cleavage of Bdi-SPL14L guided by Bdi-miR156 and Bdi-miR529 as discussed above, was precisely pinpointed at sites four nucleotides apart using PARE (Figure  9b), reminiscent of similar observations from rice PARE data [34].

Additionally, miR529, which is in the same miRNA family as miR156, has been proposed to play a crucial role in panicle branching [34].

2

MiR156, which plays a role in controlling flowering and leaf development [59] appeared targeted by several lncRNAs.

We also found TMs related to large miRNA families such as miR156, miR395, miR399 and miR5174.

3

Some of these miRNAs including miR156, miR160, miR166 and mi171 are deeply conserved, even in lower plants such as Physcomitrella patens [45].

For example, the number of family members for miR156, miR160, miR164, miR166, miR167, miR171 and miR396 in Brachypodium was similar to that in Arabidopsis, but much higher in rice and Populus (Table 4).

For example, seven families (miR156, miR160, miR164, miR166, miR167, miR171 and miR396) had similar number of members in Brachypodium and Arabidopsis, but their sizes were much larger in rice and Populus (Table 4).

4

Because miR156 and miR172 participate in the age -dependent regulation of flowering in diverse plants 12 13, it will be interesting to explore whether alterations in FT2 growth-related AS in B. distachyon is controlled by these two miRNAs during flowering processes.

5

In Arabidopsis, the LFY, FUL and AP1 genes are activated by SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 (SPL3) (Figure 1) which is regulated by FT and microRNA156 [100].