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![]() 2 publications mentioning cbr-mir-124bOpen access articles that are associated with the species Caenorhabditis briggsae and mention the gene name mir-124b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: cel-mir-1, mmu-mir-1a-1, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-206, cel-mir-124, cel-mir-228, hsa-mir-1-2, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-206, cbr-mir-1, cbr-mir-124a, cbr-mir-228, hsa-mir-1-1, mmu-mir-1a-2, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-1b, cbr-mir-124c, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3
The down regulation of brain mRNAs by mir-124 in transfected HeLa cells suggests that this miRNA confers tissue specific expression.
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Mir-1 is known to have high expression levels in mammalian heart and muscle cells while mir-124 contributes to the cells of the nervous system [24].
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Mir-124 has also been shown to promote neuronal differentiation by down regulating the general splice regulator PTB1 [25].
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This motif was also found to be especially abundant in the upstream sequences of two old and biologically important miRNAs, mir-1 and mir-124, thus suggesting a connection between the number of motif instances in the upstream sequence close to a miRNA start site and a globally conserved function of the miRNA.
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The abundance and conservation of this motif in the upstream of two old and important miRNAs, mir-1 and mir-124 suggest a connection to miRNAs with global specialized function.
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Similar results were observed when looking at the occurrences of GANNNNGA in the mir-124 -family, where the putative promoter sequence of mir-124a-1 is the only one that includes a significant number of the motif in both species, 37 and 19, respectively.
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Figure 4The frequency diagrams of the motif GANNNNGA occurrences in sequences 1000 bp upstream of mir-1 (panel a) and mir-124 (panel b) family members in C. elegans, C. briggsae, human and mouse.
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The mir-1-1 and mir-124-a1 1000 bp upstream sequences of human and mouse contain similar number of occurrences of the motif GANNNNGA as the corresponding sequences in C. elegans and C. briggsae.
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In both cases, we chose mir-1-1 as the representative of mir-1 -family and mir-124a-1 for the representative of mir-124 -family for human and mouse.
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We found these motifs also from the mir-1 and mir-124 1000 bp upstream sequences in C. elegans and C. briggsae, thus strengthening the connection of these miRNAs with a muscle specific function in these two worms.
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To determine whether the motif GANNNGA is conserved in the upstream sequences of mir-1, mir-124 and mir-228 orthologs in other species, we located all its occurrences from 1000 bp upstream sequences of all miRNAs that, according to miRBase [17], belong to mir-1 or mir-124 family in human and mouse genomes.
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When comparing these results, we found that the motif GANNNNGA is especially abundant in the 1000 bp upstream sequences of miRNAs mir-1, mir-124 and mir-228 in both worms.
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The multiple sequence alignment of the mir-124 family upstream sequences of C. elegans, C. briggsae, human and mouse.
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In addition, this motif was observed to be most abundant in the upstream sequences of two important miRNAs, mir-1 and mir-124.
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We also found the frequency distribution of the motif in the upstream sequences from human and mouse orthologs of mir-1 and mir-124 to approximately follow its corresponding distributions in the two worms, thus confirming the conserved nature of the motif.
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Mir-228 is not found from these two genomes, and generally it is included in the mir-124 family [18].
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The 1000 bp upstream sequences of human and mouse miRNAs that belong to mir-1 and mir-124 families were downloaded from UCSC Genome Browser [40], and the multiple sequence alignments for the mir-1 and mir-124 families upstream sequences were made with ClustalW [37].
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Click here for file The multiple sequence alignment of the mir-124 family upstream sequences of C. elegans, C. briggsae, human and mouse.
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We draw the frequency diagrams of the motif GANNNNGA in the 1000 bp upstream sequences of mir-1 and mir-124 orthologs in these four species (Figure 4), and made the global alignments of mir-1 and mir-124 upstream sequences (Additional files 1 and 2).
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Prior to and independent from the current study, there has been keen interest in mir-1 and mir-124, two miRNAs with the most abundant GANNNNGA motifs.
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Other miRNAs from this paper: cel-let-7, cel-mir-1, cel-mir-2, cel-mir-43, cel-mir-75, cel-mir-79, cel-mir-80, cel-mir-81, cel-mir-82, cel-mir-86, cel-mir-87, dme-mir-1, dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-4, cel-mir-124, cel-mir-233, cel-mir-248, cel-mir-273, dme-mir-184, dme-mir-277, dme-mir-124, dme-mir-79, dme-mir-87, dme-bantam, dme-let-7, dme-mir-2c, cbr-let-7, cbr-mir-1, cbr-mir-43, cbr-mir-75, cbr-mir-79, cbr-mir-80, cbr-mir-81, cbr-mir-82, cbr-mir-86, cbr-mir-87, cbr-mir-124a, cbr-mir-233, cbr-mir-248, cel-mir-356a, dps-bantam, dps-let-7, dps-mir-1, dps-mir-2a-1, dps-mir-2a-2, dps-mir-2b-1, dps-mir-2b-2, dps-mir-2c, dps-mir-4, dps-mir-79, dps-mir-87, dps-mir-124, dps-mir-184, dps-mir-277, cbr-mir-356, cel-mir-356b, cbr-mir-2, cbr-mir-124c
Indeed, decreased mRNA levels (in human HeLa cells) were observed for dozens of genes upon transfection of two distinct miRNAs, miR-1 and miR-124; it was also shown that the 3′ untranslated regions (UTRs) of these down-regulated mRNAs have significant complementarity to the 5′ extremity of the transfected miRNAs.
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