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5 publications mentioning mdm-MIR858

Open access articles that are associated with the species Malus domestica and mention the gene name MIR858. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 172
Other miRNAs from this paper: mdm-MIR156a, mdm-MIR156b, mdm-MIR156c, mdm-MIR156d, mdm-MIR156e, mdm-MIR156f, mdm-MIR156g, mdm-MIR156h, mdm-MIR156i, mdm-MIR156j, mdm-MIR156k, mdm-MIR156l, mdm-MIR156m, mdm-MIR156n, mdm-MIR156o, mdm-MIR156p, mdm-MIR156q, mdm-MIR156r, mdm-MIR156s, mdm-MIR156t, mdm-MIR156u, mdm-MIR156v, mdm-MIR156w, mdm-MIR156x, mdm-MIR156y, mdm-MIR156z, mdm-MIR156aa, mdm-MIR156ab, mdm-MIR156ac, mdm-MIR156ad, mdm-MIR156ae, mdm-MIR159a, mdm-MIR159b, mdm-MIR162a, mdm-MIR162b, mdm-MIR164a, mdm-MIR164b, mdm-MIR164c, mdm-MIR164d, mdm-MIR164e, mdm-MIR164f, mdm-MIR167a, mdm-MIR167b, mdm-MIR167c, mdm-MIR167d, mdm-MIR167e, mdm-MIR167f, mdm-MIR167g, mdm-MIR167h, mdm-MIR167i, mdm-MIR167j, mdm-MIR168a, mdm-MIR168b, mdm-MIR172a, mdm-MIR172b, mdm-MIR172c, mdm-MIR172d, mdm-MIR172e, mdm-MIR172f, mdm-MIR172g, mdm-MIR172h, mdm-MIR172i, mdm-MIR172j, mdm-MIR172k, mdm-MIR172l, mdm-MIR172m, mdm-MIR172n, mdm-MIR172o, mdm-MIR319a, mdm-MIR319b, mdm-MIR390a, mdm-MIR390b, mdm-MIR390c, mdm-MIR390d, mdm-MIR390e, mdm-MIR390f, mdm-MIR396a, mdm-MIR396b, mdm-MIR396c, mdm-MIR396d, mdm-MIR396e, mdm-MIR396f, mdm-MIR396g, mdm-MIR399a, mdm-MIR399b, mdm-MIR399c, mdm-MIR399d, mdm-MIR399e, mdm-MIR399f, mdm-MIR399g, mdm-MIR399h, mdm-MIR399i, mdm-MIR399j, mdm-MIR2111a, mdm-MIR2111b, mdm-MIR3627a, mdm-MIR3627b, mdm-MIR3627c, mdm-MIR535a, mdm-MIR535b, mdm-MIR535c, mdm-MIR535d, mdm-MIR828a, mdm-MIR828b, mdm-MIR159c, mdm-MIR319c, mdm-MIR3627d, mdm-MIR159d, mdm-MIR159e, mdm-MIR159f, mdm-MIR399k, mdm-MIR319d, mdm-MIR319e, mdm-MIR319f, mdm-MIR319g, mdm-MIR319h, mdm-MIR172p
To address the possibility that some MYB gene targets were missed during degradome analysis, possibly due to inactive or low levels of target gene expression in the plant tissues analyzed, we performed target prediction analysis in over 400 putative apple MYBs and identified an additional 8, 15 and 42 MYB genes with a cleavage-favorable alignment score (≤5) for miR159, miR828 and miR858, respectively. [score:9]
The remaining 35 targets identified were shared by miR828 and miR858, with the former targeting four MYB genes and MdTAS4 and the latter targeting up to 30 genes, including 24 coding for MYB factors, 2 coding for mate efflux proteins and 3 coding for lipases (Table 2; Table S6 in Additional file 1). [score:7]
That miR828 and miR858 targeted substantially different numbers of MYB genes despite the adjacent location of their target sites prompted us to examine conservation profiles of their target sequences at both the amino acid and nucleotide levels (Figure 4). [score:7]
The miR159 target site was found to locate in the sequence-divergent region, while the miR858 and miR828 target sites both mapped to a 55-nucleotide region in the conserved coding region upstream of the divergent region, and the two sites were separated by a 12-nucleotide fragment with the position of the miR858 target site at the 5' end and that of miR828 at the 3' end (Figure 3b). [score:7]
Although the footprint of the 55-nucleotide sequence encompassing both miR828 and miR858 target sites is detected in dicot and monocot species (Figure 3b), miR828 and miR858 emerged only in dicot species [41], indicating that miR828- and miR858 -mediated regulation of MYB genes is a feature of dicot species, consistent with our finding that miR828 and miR858 target a large number of MYBs in both apple and Arabidopsis (Figure 3a). [score:6]
miR828 was specifically expressed in flower while miR858 accumulated in all tissues tested, but was found to be most abundant in mature fruit (Figure 3c), suggesting that miR828 and miR858 differentially regulated their co -targeted MYBs in different tissues. [score:6]
The finding that miR828 and miR858 co -targeted a group of MYB genes prompted us to examine whether they were co-expressed or differentially regulated among apple tissues. [score:6]
All the apple MYB targets for miR828, miR858, and miR159 were predicted by Targetfinder 1.6 with the alignment score no more than 5. Amino acid sequences of 126 R2R3 and 5 R1R2R3 MYB factors in Arabidopsis were retrieved from TAIR [82]and the phylogenetic tree was inferred using the neighbor-joining method and 1,000 bootstraps with putative full-length sequences using CLUSTAL X2 [81]. [score:5]
The alignment score threshold was set to 4.5 for conserved and less conserved miRNAs (except for two ARF targets of miR167 and two MYB targets of miR858, for which the score was 5) and to 5 for novel and candidate miRNAs. [score:5]
Since pairing between the miRNA seed region and corresponding target site is critical for miRNA cleavage [62], the level of sequence conservation in this region could impact miR828- and miR858 -targeted MYB populations. [score:5]
Correspondingly, the miR858 target site was found to be more conserved than the miR828 target site at the nucleotide level in both apple and Arabidopsis (Figure 4e; Figure S3b in Additional file 2). [score:5]
Of the 18 amino acid residues, the first seven (1 to 7) encoded by the 21-nucleotide miR858 target site were located in the highly conserved region covering three amino acid residues upstream and four amino acid residues at the 5' end of H3 while most of the last seven (12 to 18) encoded by the miR828 target site were located in the much less conserved region downstream of H3 (Figure 4c, d). [score:5]
However, a few of the known miRNAs, including miR319 and miR396, were found to target additional genes in apple that have not been previously reported, while others like miR828 and miR858 target an unexpectedly large number of MYB genes. [score:5]
Consistent with this prediction, miR828 and miR858 target sites, which overlap the conserved R3 region, are found in more MYBs than the miR159 target site located in the divergent region (Figure 3a). [score:5]
miR828 and miR858 have been shown to target MYBs in other species but their target number was very limited [36, 55]. [score:5]
This difference was particularly pronounced in a region (positions 10 to 20 in the miR858 target site, and 44 to 54 in the miR828 target site) that specifically pairs with the miRNA seed region (positions 2 to 12) (Figure 4e; Figure S3b in Additional file 2). [score:5]
We also found that miR858 shared 11 targets with miR828 and two with miR159 (Figure 3a; Table S7 in Additional file 1), but no common target was identified for miR828 and miR159. [score:5]
The 66 MYBs targeted by miR858 represent at least 14 subgroups shown to regulate diverse biological processes and metabolism pathways relevant to cell wall formation, lignification, anthocyanin biosynthesis, cell fate and identity, plant development and response to biotic and abiotic stresses in Arabidopsis. [score:5]
Importantly, we found that miR159, miR828 and miR858 can collectively target up to 81 MYB genes potentially involved in diverse aspects of plant growth and development. [score:4]
The detection of differential expression of miR828 and miR858 among various tissues appears to support their different regulatory roles (Figure 3c). [score:4]
Finding an unusually large number of MYB targets for miR828 and miR858 suggests that they gained more diverse and broad regulatory roles in apple. [score:4]
The latter possibility is favored by the fact that 10 out of 11 MYBs co -targeted by miR828 and miR858 are related to regulation of anthocyanin biosynthesis. [score:4]
Nine of the ten MYBs co -targeted by miR828 and miR858 cluster together within subgroup 5, which is involved in the regulation of proanthocyanidin biosynthesis (Figure 3d). [score:4]
Figures 1b and 3c show that miR828 and miR858 exhibit a distinct expression pattern that was generally corroborated by boths and RNA sequencing. [score:3]
These results imply that the targeting capacity of miR858 and miR828 in apple and Arabidopsis might be even broader than those reported in Figure 3a, especially for miR858. [score:3]
Figure S4: T-plots for targets of miR828, miR858 and tasiARF. [score:3]
Strikingly, this 55-nucleotide fragment encompassing the miR858 and miR828 targeted sequences and 12-nucleotide spacer was found to be highly conserved across a wide range of dicots and monocots (Figure 3b). [score:3]
The co -targeting sequence of miR828 and miR858 is located in the region encoding the conserved R3 repeat domain of MYB proteins. [score:3]
Figure 4 The co -targeting sequence of miR858 and miR828 is located in the region encoding the R3 repeat domain of MYB proteins. [score:3]
Based on alignment scores ≤5, 66 and 19 apple MYBs were identified to be targeted by miR858 and miR828, respectively (Figure 3a). [score:3]
The dual cleavage by miR858 and miR828 was confirmed in one of the targeted MYBs (MDP0000124555) by RNA ligation -mediated 5' rapid amplification of cDNA ends (RLM-5'-RACE) analysis (Figure 3b). [score:3]
In Arabidopsis, miR159, miR828 and miR858 were either predicted or confirmed to target at least 13 MYB genes [56, 57]. [score:3]
Intriguingly, miR858 was found to co-target 11 MYBs with miR828 and two MYBs with miR159 (Figure 3a), raising the question of whether the convergence of two miRNAs upon the same MYB genes is an evolutionary coincidence or conveys some biological significance. [score:3]
Pairing of miR828 and miR858 with their target sites in a representative MYB transcript is illustrated below. [score:3]
Similarly, the miR858 target site, which overlaps the more highly conserved 5' end of the H3 domain, is conserved in more MYBs than the miR828 site, which overlaps the less conserved 3' end of the H3 domain (Figure 4c-4e). [score:3]
We found that the18 amino acid polypeptide encoded by the 55-nucleotide sequence that bears both miR828 and miR858 target sites was located in the conserved R3 DNA binding domain of MYB factors (Figure 4a). [score:3]
Thus, a total of nine MYBs for miR159, 19 for miR828 and 66 for miR858 were found, bringing the total number of MYBs potentially regulated by these miRNAs to 81 (Figure 3a; Table S7 in Additional file 1). [score:2]
Figure 3Complex MYB regulatory network mediated by miR159, miR828, and miR858. [score:2]
miR828 and miR858 may either redundantly reinforce each other's silencing function or differentially regulate anthocyanin accumulation in various apple tissues. [score:2]
Thus, the roles of miR858 -mediated regulation of MYBs in apple are predicted to be much broader than those for either miR828 or miR159. [score:2]
Therefore, we further analyzed the alignment score distribution profiles for these two miRNAs among all the 251 apple MYBs, and found 95 of the 251 MYBs with a less cleavage-favorable alignment score (> 5 and ≤7) and 90 with a cleavage-unfavorable alignment score (> 7) with miR858 (Figure 4b, top). [score:1]
We found that while blotting results for some miRNAs - miR828, miR858, miR390 (Figure 3c) and miR4376 (Figure 1c) - were reflective of the relative abundances of sequenced RNAs from these four tissues, many others displayed varying degrees of divergence between the two analyses. [score:1]
[1 to 20 of 42 sentences]
2
[+] score: 66
Other miRNAs from this paper: mdm-MIR156a, mdm-MIR156b, mdm-MIR156c, mdm-MIR156d, mdm-MIR156e, mdm-MIR156f, mdm-MIR156g, mdm-MIR156h, mdm-MIR156i, mdm-MIR156j, mdm-MIR156k, mdm-MIR156l, mdm-MIR156m, mdm-MIR156n, mdm-MIR156o, mdm-MIR156p, mdm-MIR156q, mdm-MIR156r, mdm-MIR156s, mdm-MIR156t, mdm-MIR156u, mdm-MIR156v, mdm-MIR156w, mdm-MIR156x, mdm-MIR156y, mdm-MIR156z, mdm-MIR156aa, mdm-MIR156ab, mdm-MIR156ac, mdm-MIR156ad, mdm-MIR156ae, mdm-MIR159a, mdm-MIR159b, mdm-MIR160a, mdm-MIR160b, mdm-MIR160c, mdm-MIR160d, mdm-MIR160e, mdm-MIR162a, mdm-MIR162b, mdm-MIR166a, mdm-MIR166b, mdm-MIR166c, mdm-MIR166d, mdm-MIR166e, mdm-MIR166f, mdm-MIR166g, mdm-MIR166h, mdm-MIR166i, mdm-MIR167a, mdm-MIR167b, mdm-MIR167c, mdm-MIR167d, mdm-MIR167e, mdm-MIR167f, mdm-MIR167g, mdm-MIR167h, mdm-MIR167i, mdm-MIR167j, mdm-MIR171a, mdm-MIR171b, mdm-MIR171c, mdm-MIR171d, mdm-MIR171e, mdm-MIR171f, mdm-MIR171g, mdm-MIR171h, mdm-MIR171i, mdm-MIR171j, mdm-MIR171k, mdm-MIR171l, mdm-MIR171m, mdm-MIR171n, mdm-MIR172a, mdm-MIR172b, mdm-MIR172c, mdm-MIR172d, mdm-MIR172e, mdm-MIR172f, mdm-MIR172g, mdm-MIR172h, mdm-MIR172i, mdm-MIR172j, mdm-MIR172k, mdm-MIR172l, mdm-MIR172m, mdm-MIR172n, mdm-MIR172o, mdm-MIR319a, mdm-MIR319b, mdm-MIR393a, mdm-MIR393b, mdm-MIR393c, mdm-MIR398a, mdm-MIR398b, mdm-MIR398c, mdm-MIR399a, mdm-MIR399b, mdm-MIR399c, mdm-MIR399d, mdm-MIR399e, mdm-MIR399f, mdm-MIR399g, mdm-MIR399h, mdm-MIR399i, mdm-MIR399j, mdm-MIR3627a, mdm-MIR3627b, mdm-MIR3627c, mdm-MIR391, mdm-MIR535a, mdm-MIR535b, mdm-MIR535c, mdm-MIR535d, mdm-MIR827, mdm-MIR5225c, mdm-MIR159c, mdm-MIR5225a, mdm-MIR5225b, mdm-MIR319c, mdm-MIR7125, mdm-MIR7126, mdm-MIR393d, mdm-MIR393e, mdm-MIR393f, mdm-MIR171o, mdm-MIR7128, mdm-MIR1511, mdm-MIR3627d, mdm-MIR159d, mdm-MIR159e, mdm-MIR159f, mdm-MIR166j, mdm-MIR399k, mdm-MIR319d, mdm-MIR319e, mdm-MIR319f, mdm-MIR319g, mdm-MIR171p, mdm-MIR393g, mdm-MIR393h, mdm-MIR319h, mdm-MIR171q, mdm-MIR172p
The expression patterns of these miRNAs and their targets could be divided into four types: (1) mdm-miR156, mdm-miR160, mdm-miR535 and their targets, the SBP, AP2, AP2-like, ARF16, AFB, DC19 and RD19 genes, had the highest expression levels in roots but relatively low expression levels in fruit (Figure  9A,B,D,F and H); (2) mdm-miR393, mdm-miR398a, mdm-miR398b and their targets, the SPL2, SPL9 and ACA8 genes, were found to be expressed most abundantly in flowers but had relatively low expression levels in fruit and roots (Figure  9A,C,E,F,G and H); (3) mdm-miR172, mdm-miR162, mdm-miR162, mdm-miR5225 and their targets, the ARF17, LETM1-LIKE and ADH2 genes, showed high expression levels in leaf tissue but relatively low levels were observed in stems (Figure  9B,D,E,G and I); (4) mdm-miR858 and mdm-miR3627 and their targets, the TIR1 and MYB5 genes, had high expression levels in fruit but low levels in stems (Figure  9C,G and J). [score:25]
The expression profiles of mdm-miR162, mdm-miR535, mdm-miR858, mdm-miR3727 and miR5225 were up-regulated in J leaves compared with A leaves during leaf development, while mdm-miR398a and mdm-miR398b were down-regulated in J leaves compared with A leaves, implying that these miRNAs may participate in the regulating leaf development and other biological processes (Figure  7F,G,H and I). [score:10]
The MYB domain protein 65 was targeted by both mdm-miR159 and mdm-miR319, and the autoinhibited Ca2 + −ATPase was regulated by both mdm-miR858 and mdm-miR3627. [score:6]
Expression of miRNAs and their targets in different tissue: mdm-miR156 (A); mdm-miR172 (B); mdm-miR393 (C); mdm-miR160 (D); mdm-miR162 (E); mdm-miR535 (F); mdm-miR3627 and mdm-miR5225 (G); mdm-miR398a (H); mdm-miR398b (I); and mdm-miR858 (J). [score:5]
The expression of miRNAs and their targets in A and J leaves: mdm-miR156 (A); mdm-miR172 (B); mdm-miR393 (C); mdm-miR160 (D); mdm-miR162 (E); mdm-miR535 (F); mdm-miR3627and mdm-miR5225 (G); mdm-miR398a (H); mdm-miR398b (I); and mdm-miR858 (J). [score:5]
The expression patterns of miR858 and their targets (MYB family) were also similar to those found in apple [22] and peach trees (Figures  8 and 9) [36]. [score:5]
The expression of miRNAs and their targets in leaves of different ages: mdm-miR156 (A); mdm-miR172 (B); mdm-miR393 (C); mdm-miR160 (D); mdm-miR162 (E); mdm-miR535 (F); mdm-miR3627 and mdm-miR5225 (G); mdm-miR398a (H); mdm-miR398b (I); and mdm-miR858 (J). [score:5]
The known miRNA targets included some TFs, including SPL2 (mdm-miR156), SPL9 (mdm-miR156), ARF16 (mdm-miR160) and MYB5 (mdm-miR858), and others contained several regulatory proteins, including the LETM1-LIKE protein (mdm-miR162), AUX signaling F-box 2 protein (mdm-miR393) and the AT hook motif DNA -binding family protein (mdm-miR3627) (Table  3). [score:4]
Seven of the known miRNA families, mdm-miR1511, mdm-miR391, mdm-miR7125, mdm-miR7126, mdm-miR7128, mdm-miR827 and mdm-miR858, had only one member (Figure  4). [score:1]
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3
[+] score: 52
Other miRNAs from this paper: mdm-MIR482a, mdm-MIR156a, mdm-MIR156b, mdm-MIR156c, mdm-MIR156d, mdm-MIR156e, mdm-MIR156f, mdm-MIR156g, mdm-MIR156h, mdm-MIR156i, mdm-MIR156j, mdm-MIR156k, mdm-MIR156l, mdm-MIR156m, mdm-MIR156n, mdm-MIR156o, mdm-MIR156p, mdm-MIR156q, mdm-MIR156r, mdm-MIR156s, mdm-MIR156t, mdm-MIR156u, mdm-MIR156v, mdm-MIR156w, mdm-MIR156x, mdm-MIR156y, mdm-MIR156z, mdm-MIR156aa, mdm-MIR156ab, mdm-MIR156ac, mdm-MIR156ad, mdm-MIR156ae, mdm-MIR160a, mdm-MIR160b, mdm-MIR160c, mdm-MIR160d, mdm-MIR160e, mdm-MIR164a, mdm-MIR164b, mdm-MIR164c, mdm-MIR164d, mdm-MIR164e, mdm-MIR164f, mdm-MIR167a, mdm-MIR167b, mdm-MIR167c, mdm-MIR167d, mdm-MIR167e, mdm-MIR167f, mdm-MIR167g, mdm-MIR167h, mdm-MIR167i, mdm-MIR167j, mdm-MIR168a, mdm-MIR169a, mdm-MIR169b, mdm-MIR169c, mdm-MIR169d, mdm-MIR171a, mdm-MIR171b, mdm-MIR171c, mdm-MIR171d, mdm-MIR171e, mdm-MIR171f, mdm-MIR171g, mdm-MIR171h, mdm-MIR171i, mdm-MIR171j, mdm-MIR171k, mdm-MIR171l, mdm-MIR171m, mdm-MIR171n, mdm-MIR172a, mdm-MIR172b, mdm-MIR172c, mdm-MIR172d, mdm-MIR172e, mdm-MIR172f, mdm-MIR172g, mdm-MIR172h, mdm-MIR172i, mdm-MIR172j, mdm-MIR172k, mdm-MIR172l, mdm-MIR172m, mdm-MIR172n, mdm-MIR172o, mdm-MIR393a, mdm-MIR393b, mdm-MIR393c, mdm-MIR395a, mdm-MIR395b, mdm-MIR395c, mdm-MIR395d, mdm-MIR395e, mdm-MIR395f, mdm-MIR395g, mdm-MIR395h, mdm-MIR395i, mdm-MIR396a, mdm-MIR396b, mdm-MIR396c, mdm-MIR396d, mdm-MIR396e, mdm-MIR396f, mdm-MIR396g, mdm-MIR397a, mdm-MIR397b, mdm-MIR398a, mdm-MIR398b, mdm-MIR398c, mdm-MIR399a, mdm-MIR399d, mdm-MIR399i, mdm-MIR408a, mdm-MIR3627a, mdm-MIR3627b, mdm-MIR3627c, mdm-MIR391, mdm-MIR477b, mdm-MIR477a, mdm-MIR482b, mdm-MIR482c, mdm-MIR535a, mdm-MIR535b, mdm-MIR535c, mdm-MIR535d, mdm-MIR827, mdm-MIR828a, mdm-MIR828b, mdm-MIR408b, mdm-MIR408c, mdm-MIR408d, mdm-MIR482d, mdm-MIR7121a, mdm-MIR7121b, mdm-MIR7121c, mdm-MIR7121d, mdm-MIR7121e, mdm-MIR7121f, mdm-MIR7121g, mdm-MIR7121h, mdm-MIR5225c, mdm-MIR7124a, mdm-MIR5225a, mdm-MIR5225b, mdm-MIR7125, mdm-MIR393d, mdm-MIR393e, mdm-MIR393f, mdm-MIR7127a, mdm-MIR7127b, mdm-MIR171o, mdm-MIR169e, mdm-MIR169f, mdm-MIR3627d, mdm-MIR395j, mdm-MIR169g, mdm-MIR169h, mdm-MIR169i, mdm-MIR169j, mdm-MIR171p, mdm-MIR393g, mdm-MIR393h, mdm-MIR395k, mdm-MIR171q, mdm-MIR169k, mdm-MIR169l, mdm-MIR169m, mdm-MIR169n, mdm-MIR172p, mdm-MIR395l, mdm-MIR169o
The expression levels of “Granny Smith” mdm-miR858 in the treatment group were decreased at 1 day, and then rapidly up-regulated at 2 days, finally down-regulated to lowest level at 6 days. [score:9]
The expression profiles of MdSPL9 (MDP0000297978), MdbHLH (MDP0000225680), MdMYB9 (MDP0000210851), and MdANR2 (MDP0000320264) target genes regulated by mdm-miR156, mdm-miR828, mdm-miR858 and miR5072 were analyzed, respectively. [score:6]
It has been reported that miR828 and miR858 could directly or indirectly control anthocyanin biosynthesis in apple, and differentially expressed miR156 can positively regulate anthocyanin biosynthesis by the SPL transcription factor in Arabidopsis thaliana (Gou et al., 2011; Xia et al., 2012). [score:6]
MdMYB transcription factors and anthocyanin regulatory C1 protein-like were found to serve as the target genes of miR828 and miR858. [score:4]
Although the expression levels of mdm-miR858 slightly fluctuated in the “Starkrimson” treatment group, transcription levels of MdMYB9 transcription factor increased to 40-fold at 6 days after debagging, which was in agreement with trends of increasing anthocyanin concentrations. [score:3]
Previous study shows that miR828 is specifically expressed in apple flowers, but miR858 accumulates most abundantly in mature apple fruit (Xia et al., 2012), in agreement with our results that the normalized reads of miR858 were 120-fold higher than that of miR828. [score:3]
Interestingly, in our study, MdMYB9 transcription factor was predicted to be targeted by mdm-miR858. [score:3]
While differentially-expressed miR172 and miR398 were only present in the T1/N1 group, miR167, miR391, miR7121, and miR858 were only in the T2/N2 group, and miR535 and miR7127 only in the T3/N3 group. [score:3]
Interestingly, our results found that the expression levels of mdm-miR156, mdm-miR828 and mdm-miR858 were different when fruits were debagged. [score:3]
The mRNA abundances of mdm-miR858 and miR5072 in the “Granny Smith” control group and the “Starkrimson” treatment group were slightly fluctuated at low expression level. [score:3]
The expression levels of four known miRNAs (mdm-miR156, mdm-miR828, mdm-miR858, and miR5072) and their target genes were investigated by using qRT-PCR (Figures 7, 8). [score:3]
We found that the expression of miR858 actually reversed to MdMYB9 transcription factor, implying that mdm-miR858 might be negatively correlated with the accumulation of anthocyanin and PA in apple peel. [score:3]
It was suggested that the regulation of mdm-miR858/ MdMYB9 might be involved in anthocyanin biosynthesis in “Granny Smith” apple peel after debagging. [score:2]
It can be seen that transcription level of MdMYB9 transcription factor caused by mdm-miR858 was more readily influenced in “Starkrimson” than that in “Granny Smith,” and mdm-miR858 played an important role in anthocyanin accumulation in both cultivars. [score:1]
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4
[+] score: 14
Other miRNAs from this paper: mdm-MIR482a, mdm-MIR156a, mdm-MIR156b, mdm-MIR156c, mdm-MIR156d, mdm-MIR156e, mdm-MIR156f, mdm-MIR156g, mdm-MIR156h, mdm-MIR156i, mdm-MIR156j, mdm-MIR156k, mdm-MIR156l, mdm-MIR156m, mdm-MIR156n, mdm-MIR156o, mdm-MIR156p, mdm-MIR156q, mdm-MIR156r, mdm-MIR156s, mdm-MIR156t, mdm-MIR156u, mdm-MIR156v, mdm-MIR156w, mdm-MIR156x, mdm-MIR156y, mdm-MIR156z, mdm-MIR156aa, mdm-MIR156ab, mdm-MIR156ac, mdm-MIR156ad, mdm-MIR156ae, mdm-MIR159a, mdm-MIR159b, mdm-MIR160a, mdm-MIR160b, mdm-MIR160c, mdm-MIR160d, mdm-MIR160e, mdm-MIR164a, mdm-MIR164b, mdm-MIR164c, mdm-MIR164d, mdm-MIR164e, mdm-MIR164f, mdm-MIR166a, mdm-MIR166b, mdm-MIR166c, mdm-MIR166d, mdm-MIR166e, mdm-MIR166f, mdm-MIR166g, mdm-MIR166h, mdm-MIR166i, mdm-MIR167a, mdm-MIR167b, mdm-MIR167c, mdm-MIR167d, mdm-MIR167e, mdm-MIR167f, mdm-MIR167g, mdm-MIR167h, mdm-MIR167i, mdm-MIR167j, mdm-MIR168a, mdm-MIR168b, mdm-MIR169a, mdm-MIR169b, mdm-MIR169c, mdm-MIR169d, mdm-MIR171a, mdm-MIR171b, mdm-MIR171c, mdm-MIR171d, mdm-MIR171e, mdm-MIR171f, mdm-MIR171g, mdm-MIR171h, mdm-MIR171i, mdm-MIR171j, mdm-MIR171k, mdm-MIR171l, mdm-MIR171m, mdm-MIR171n, mdm-MIR172a, mdm-MIR172b, mdm-MIR172c, mdm-MIR172d, mdm-MIR172e, mdm-MIR172f, mdm-MIR172g, mdm-MIR172h, mdm-MIR172i, mdm-MIR172j, mdm-MIR172k, mdm-MIR172l, mdm-MIR172m, mdm-MIR172n, mdm-MIR172o, mdm-MIR393a, mdm-MIR393b, mdm-MIR393c, mdm-MIR395a, mdm-MIR395b, mdm-MIR395c, mdm-MIR395d, mdm-MIR395e, mdm-MIR395f, mdm-MIR395g, mdm-MIR395h, mdm-MIR395i, mdm-MIR396a, mdm-MIR396b, mdm-MIR396c, mdm-MIR396d, mdm-MIR396e, mdm-MIR396f, mdm-MIR396g, mdm-MIR397a, mdm-MIR397b, mdm-MIR399a, mdm-MIR399b, mdm-MIR399c, mdm-MIR399d, mdm-MIR399e, mdm-MIR399f, mdm-MIR399g, mdm-MIR399h, mdm-MIR399i, mdm-MIR399j, mdm-MIR391, mdm-MIR482b, mdm-MIR482c, mdm-MIR535a, mdm-MIR535b, mdm-MIR535c, mdm-MIR535d, mdm-MIR827, mdm-MIR828a, mdm-MIR828b, mdm-MIR482d, mdm-MIR7123a, mdm-MIR7123b, mdm-MIR5225c, mdm-MIR159c, mdm-MIR7124a, mdm-MIR7124b, mdm-MIR5225a, mdm-MIR5225b, mdm-MIR7125, mdm-MIR7126, mdm-MIR393d, mdm-MIR393e, mdm-MIR393f, mdm-MIR171o, mdm-MIR169e, mdm-MIR169f, mdm-MIR7128, mdm-MIR1511, mdm-MIR159d, mdm-MIR159e, mdm-MIR159f, mdm-MIR166j, mdm-MIR399k, mdm-MIR395j, mdm-MIR169g, mdm-MIR169h, mdm-MIR169i, mdm-MIR169j, mdm-MIR171p, mdm-MIR393g, mdm-MIR393h, mdm-MIR395k, mdm-MIR171q, mdm-MIR169k, mdm-MIR169l, mdm-MIR169m, mdm-MIR169n, mdm-MIR172p, mdm-MIR395l, mdm-MIR169o
In contrast, five members of mdm-miR164, three members of mdm-miR171, six members of mdm-miR172, three members of mdm-miR393, nine members of mdm-miR395, two members of mdm-miR396, six members of mdm-miR399, two members of mdm-miR5225, two members of mdm-miR7124, and mdm-miR858 were all downregulated in YF shoot tips. [score:4]
miRNA828 and miRNA858 target the MYB family at the R3 domian (Xia et al., 2012; Figure 6B). [score:3]
The potential targets of miR858, miR828, and miR156 participate in the fatty acid biosynthetic process. [score:3]
The potential targets of miR159, miR166, miR167, miR171, miR172, miR393, miR858, and miR828 are involved in cell growth. [score:3]
mdm-miR1511, mdm-miR391, mdm-miR7125, mdm-miR7126, mdm-miR827, and mdm-miR858 only had one member. [score:1]
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5
[+] score: 4
Other miRNAs from this paper: ppe-MIR171f, ppe-MIR394a, ppe-MIR828, ppe-MIR171h, ppe-MIR171a, ppe-MIR171e, ppe-MIR171g, ppe-MIR171b, ppe-MIR171c, mdm-MIR156a, mdm-MIR156b, mdm-MIR156c, mdm-MIR156d, mdm-MIR156e, mdm-MIR156f, mdm-MIR156g, mdm-MIR156h, mdm-MIR156i, mdm-MIR156j, mdm-MIR156k, mdm-MIR156l, mdm-MIR156m, mdm-MIR156n, mdm-MIR156o, mdm-MIR156p, mdm-MIR156q, mdm-MIR156r, mdm-MIR156s, mdm-MIR156t, mdm-MIR156u, mdm-MIR156v, mdm-MIR156w, mdm-MIR156x, mdm-MIR156y, mdm-MIR156z, mdm-MIR156aa, mdm-MIR156ab, mdm-MIR156ac, mdm-MIR156ad, mdm-MIR156ae, mdm-MIR159a, mdm-MIR159b, mdm-MIR160a, mdm-MIR160b, mdm-MIR160c, mdm-MIR160d, mdm-MIR160e, mdm-MIR164a, mdm-MIR164b, mdm-MIR164c, mdm-MIR164d, mdm-MIR164e, mdm-MIR164f, mdm-MIR166i, mdm-MIR167a, mdm-MIR167b, mdm-MIR167c, mdm-MIR167d, mdm-MIR167e, mdm-MIR167f, mdm-MIR167g, mdm-MIR167h, mdm-MIR167i, mdm-MIR167j, mdm-MIR171a, mdm-MIR171b, mdm-MIR171c, mdm-MIR171d, mdm-MIR171e, mdm-MIR171f, mdm-MIR171g, mdm-MIR171h, mdm-MIR171i, mdm-MIR171j, mdm-MIR171k, mdm-MIR171l, mdm-MIR171m, mdm-MIR171n, mdm-MIR172a, mdm-MIR172b, mdm-MIR172c, mdm-MIR172d, mdm-MIR172e, mdm-MIR172f, mdm-MIR172g, mdm-MIR172h, mdm-MIR172i, mdm-MIR172j, mdm-MIR172k, mdm-MIR172l, mdm-MIR172m, mdm-MIR172n, mdm-MIR172o, mdm-MIR394a, mdm-MIR394b, mdm-MIR396e, mdm-MIR828a, mdm-MIR828b, mdm-MIR159c, mdm-MIR171o, ppe-MIR156a, ppe-MIR156b, ppe-MIR156c, ppe-MIR156d, ppe-MIR156e, ppe-MIR156f, ppe-MIR156g, ppe-MIR156h, ppe-MIR156i, ppe-MIR159, ppe-MIR160a, ppe-MIR160b, ppe-MIR164a, ppe-MIR164b, ppe-MIR164c, ppe-MIR164d, ppe-MIR167a, ppe-MIR167b, ppe-MIR167c, ppe-MIR167d, ppe-MIR171d, ppe-MIR172a, ppe-MIR172b, ppe-MIR172c, ppe-MIR172d, ppe-MIR394b, ppe-MIR858, mdm-MIR159d, mdm-MIR159e, mdm-MIR159f, mdm-MIR171p, mdm-MIR171q, mdm-MIR172p
In addition, miR828, and miR858, which are also involved in R2R3-MYB regulation[72] was predicted target MYB genes in Rosa (Additional file 2). [score:4]
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