5 publications mentioning rno-mir-1843a

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-1843a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

From P4 to P28, miRNAs displayed very similar expression between both progeny: 0–22 (<5%) miRNAs displayed differential expression and 0–7 of them (<2%) had expression differences higher than 3. Ratios of miR-7a-5p, miR-24-3p, miR-29-3p, miR-137-3p or miR-1843-5p expressions relatively to miR-124-3p expression calculated from RT-qPCR at P28 data matched those calculated from HTS data (Fig. 3B).

Given the number of miRNAs and the limited quantity of material in individual ARC/MEs, we selected six miRNAs: miR-124a-3p which is wi dely expressed throughout brain 15, miR-29a/b-3p whose brain-specific knockdown results in neuronal cell death 16 17, miR-7a-5p and miR-137-3p which are expressed in hypothalamic nuclei including ARC 18, miR-24-3p and miR-1843-5p which are still poorly or not clearly documented.

As the U6 snRNA was excluded from small RNA fractions and could not been used as an internal reference to quantify miRNA expressions, we quantified the expression of miR-7a-5p, miR-24-3p, miR-29-3p, miR-137-3p and miR-1843-5p relatively to that of miR-124-3p, at P4, P8, P14.4 and P21.4 relatively to P28, from RT-qPCR amplification or sequencing data (Fig. 3A).

Expression of miR-7a-5p, miR-24-3p, miR-29-3p, miR-137-3p and miR-1843-5p were quantified relatively to those of miR-124-3p, and relatively to those of stage P28, by using the ΔΔCt method 25 and experimentally ascertained amplification efficiencies.

2

Among them, 12 miRNAs (rno-miR-10b-5p, rno-miR-122-5p, rno-miR-184, rno-miR-1843-5p, rno-miR-196c-5p, rno-miR-199a-5p, rno-miR-202-5p, rno-miR-206-3p, rno-miR-208b-5p, rno-miR-224-5p, rno-miR-298-5p and rno-miR-31a-5p) were significantly upregulated(p<0.01, fold-change >1) compared to the control group and only rno-miR-208a-3p were significantly downregulated (p<0.01, fold-change <-1) (Fig 3).

Meanwhile, rno-miR-10b-5p, rno-miR-184, rno-miR-1843-5p, rno-miR-196c-5p, rno-miR-202-5p, rno-miR-206-3p, rno-miR-224-5p, rno-miR-298-5p and rno-miR-31a-5p were reported for the first time to be differentially expressed in HF tissue.

3

Of these, miR-500-3p, miR-23b-3p, miR-200a-3p, miR-19b-3p, miR-92a-1-5p, miR-21-5p, miR-21-3p, miR-1843-3p, miR-223-3p, miR-3473, and miR-129-2-3p were found to be upregulated, whereas miR-92b-3p, miR-3102, and miR-3577 were found to be downregulated in the rat brain.

Nevertheless, many of them had not been reported (miR-500-3p, miR-1843-3p, and miR-3473, miR-3102, and miR-3577).

4

After applying these structural criteria, 5 novel pre-miRNAs homologous to known miRNAs in other species were discovered and named as rno-mir-1839, rno-mir-3068, rno-mir-1843, rno-mir-509 and rno-mir-1306.

rno-mir-1843.

All miRNAs show a perfect 2 nt 3′ overhang, except rno-miR-3068-3p and rno-miR-1843-3p marked with (#).

The sequences and the secondary structures of these 5 novel rat homologous pre-miRNAs are shown in Figure 2. The single nucleotide extension isomirs of the mature* sequences had higher read counts than the mature* sequences in two miRNAs (rno-miR-3068-3p and rno-miR-1843-3p) (Figure 2c and 2e).

The single nucleotide extension isomirs of the mature* sequences had higher read counts than the mature* sequences with perfect 2 nt 3′ overhang in two miRNAs (rno-miR-3068-3p and rno-miR-1843-3p).

5

Putative target mRNAs of 31 miRNAs were predicted as previously mentioned (rno-mir-378b and mir-1843-5p are not found in the selected databases).