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1 publications mentioning ame-mir-6001

Open access articles that are associated with the species Apis mellifera and mention the gene name mir-6001. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 71
Other miRNAs from this paper: ame-mir-9a, ame-mir-100, ame-mir-13a, ame-mir-306
Target prediction for Bte-miR-6001-5p and Bte-miR-6001-3p showed that many putative targets for these miRNAs are genes involved in development and reproductive differentiation in Apis and Drosophila, including genes associated with ovary and oocyte development, neurodevelopment, larval development and larval moulting (Table 1). [score:9]
Other studies have shown that, in A. mellifera, Ame-miR-6001-5p was not differentially expressed between queen- and worker-destined larvae 21 and that Ame-miR-6001-3p was weakly differentially expressed between castes in larvae 21 or was differentially expressed between female and male larvae but not between castes in larvae 22. [score:7]
Second, it is possible that differential expression was present but that the low absolute levels of expression of these miRNAs (in contrast to those of the miR-6001 duplex) meant that differential expression was not detected by the Northern blots. [score:7]
Both Bte-miR-6001-5p and Bte-miR-6001-3p showed the highest degree of differential expression (16.5 and 15.8 fold change between caste phenotypes, respectively), while the other four miRNAs showed only 2.2–3.2 fold change between expression levels between caste phenotypes (Fig. 1). [score:5]
It is also consistent with higher expression of Bte-miR-6001-5p and Bte-miR-6001-3p in the cuticle of late-instar queen-destined larvae (Fig. 3), given that differentiation of queens in B. terrestris involves delayed moulting of later larval instars 28 and that, in the B. terrestris transcriptome, genes involved in larval cuticular biogenesis show elevated expression relative to other genes 41. [score:5]
We used miRanda 62 to identify a list of potential targets of the two miRNAs that had a validated pattern of differential expression (Bte-miR-6001-5p, Bte-miR-6001-3p). [score:5]
Despite validating the sequencing results for the Bte-miR-6001 duplex, the Northern blots failed to confirm expression patterns for four other B. terrestris miRNAs that were differentially expressed according to sRNA-seq (Bte-miR-13a, Bte-miR-87a, Bte-miR-100, Bte-miR-306; Fig. 2). [score:5]
In addition, a role in caste determination for Bte-miR-6001-5p and Bte-miR-6001-3p matches the finding that the predicted targets of Bte-miR-6001-5p include ecdysone -induced protein 75 and ferredoxin, both of which have been associated with responses to ecdysone in Drosophila 42 43. [score:3]
The patterns of expression of Bte-miR-6001-5p and Bte-miR-6001-3p observed in the sRNA-seq were confirmed by the Northern blots. [score:3]
We have isolated and validated two miRNAs (Bte-miR-6001-5p and Bte-miR-6001-3p) that are more highly expressed in queen- than in worker-destined late-instar larvae in B. terrestris (Figs 1and 2). [score:3]
Bte-miR-87a, Bte-miR-100, Bte-miR-6001-5p and Bte-miR-6001-3p were most highly expressed in queen-destined larvae in late but not early larval instars (Fig. 1). [score:3]
Specifically, predicted targets of Bte-miR-6001-5p included ecdysone -induced protein 75 and ferredoxin (Table 1). [score:3]
In B. terrestris, one possibility is that one or both arms of the Bte-miR-6001 duplex targets a protein-coding gene that prevents larvae from developing along the queen-destined pathway, so that, by silencing the gene, the miRNA allows larvae to develop as queens. [score:3]
Our finding that bte-mir-6001 is a mirtron within Vhdl suggests a novel link between miRNA regulation of caste determination and the Vitellogenin family of genes, including the gene Vitellogenin (Vg), to which Vhdl has sequence homology. [score:2]
The black box around mir-6001 and the fourth intron of Vhdl represents the sequence shown below each gene schematic. [score:1]
Genome scanning for the gene bte-mir-6001 against the B. terrestris genome 37 showed that this miRNA and its precursor sequence comprise the entire fourth intron of very high density lipoprotein (Vhdl, RefSeq accession number: NM_001331111.1; Fig. 4), which is a gene with homology to Vitellogenin. [score:1]
Light blue boxes represent mir-6001, green boxes represent the predicted exons of Vhdl, dark green lines represent the introns, and the purple box the neighbouring tRNA sequence in both genomes. [score:1]
We also used Northern blots to investigate the expression patterns of Bte-miR-6001-5p and Bte-miR-6001-3p in specific tissues of late-instar queen-destined larvae (head, digestive tract and outer cuticle) and in whole bodies of queen-destined larvae and pupae. [score:1]
Schematic diagram of very high density lipoprotein (Vhdl) and mir-6001 in Bombus terrestris and Apis mellifera. [score:1]
These were Bte-miR-13a, Bte-miR-87a, Bte-miR-100, Bte-miR-306, Bte-miR-6001–5p and Bte-miR-6001-3p (Fig. 1;), with the last two miRNAs being, respectively, the 5′ and 3′ arms of the Bte-miR-6001 duplex. [score:1]
In these sequences, green characters represent nucleotides at the end of the fourth and start of the fifth exons of Vhdl; red characters, the end of the intron-exon splice junctions; and blue characters, the predicted mir-6001 precursor sequence containing the miR-6001 miRNA duplex. [score:1]
Further investigations, as well as focusing on the mechanism by which the Bte-miR-6001 duplex might affect caste, on its putative targets and on Vhdl, would therefore benefit from considering how its role has been modified during the change from primitive to advanced eusociality in bees. [score:1]
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