2 publications mentioning bdi-MIR529

Open access articles that are associated with the species Brachypodium distachyon and mention the gene name MIR529. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

In our data, Bdi-miR156 and Bdi-miR529 also showed a similar differential regulation of their target gene, Bdi-SPL14L, when they were expressed (Figure  9b).

Since Bdi-miR529 and Bdi-miR156 share several target genes and may differ for others, their over -expression should be significant and, depending on the outcome, may suggest strategies to improve food or bioenergy crops.

In addition, Bdi-miR156 expression in panicle tissue was much lower than that in the other tissues, whereas Bdi-miR529 was preferentially expressed in panicle tissue.

It has been reported that the regulation of OsSPL14 expression by Osa-miR156 and Osa-miR529 may play a crucial role in both tillering and panicle-branching in rice [34, 68, 69].

To explore this regulation in Brachypodium, we first examined the expression pattern of Bdi-miR156 and Bdi-miR529.

This result implies that cleavage of BdiSPL14L during vegetative growth is predominantly miR156 -guided, whereas miR529 and miR156 regulate the same gene during reproductive development.

In contrast to miR156, the effects of over -expressing miR529 have not been examined yet for transgenic plants.

Sequence variations and differential expression patterns between miR156 and miR529 could cause their distinct function in SPL gene repression.

Additionally, miR529, which is in the same miRNA family as miR156, has been proposed to play a crucial role in panicle branching [34].

In contrast, the PARE sequence corresponding to the site of cleavage mediated by panicle-preferential miR529 was more abundant than that of miR156 in the panicle library.

In leaf tissue of three-week-old plants, a prominent PARE sequence was mapped to a cleavage site by miR156, while the PARE sequence from the site of miR529 -mediated cleavage had a very low abundance.

The distinct cleavage of Bdi-SPL14L guided by Bdi-miR156 and Bdi-miR529 as discussed above, was precisely pinpointed at sites four nucleotides apart using PARE (Figure  9b), reminiscent of similar observations from rice PARE data [34].

2

The miR160a, miR164d, miR169f, miR172a, miR172b, miR319, miR390, miR393, miR394, miR395a, miR397a, miR529 and miR827 were moderately expressed, and were represented by the number of sequences varying between 10 and 100.

MiR528 and miR529, which are species-specific miRNAs, were also found in the NC library.

According to miRBase and published papers, miR528 only appears in Oryza sativa and miR529 has been identified in Eschscholzia californica [46], Oryza sativa, Physcomitrella patens as well as some Pinus species [46].

Similarly, in the WC library miR529 had 268 reads of the miRNA and 11 reads of the miRNA*.

The miR529* was reported to be sequenced more often than the miR529 in rice and the annotated miR529*, therefore, is suspected to be the true miRNA [26].

Our data indicate that miR529 is the true miRNA and the existing annotation should not be changed.

For miR529, there were 631 reads of the miRNA and 17 reads of the miRNA* in the NC library.