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5 publications mentioning hvu-MIR5048a

Open access articles that are associated with the species Hordeum vulgare and mention the gene name MIR5048a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 63
Other miRNAs from this paper: hvu-MIR159b, hvu-MIR159a, hvu-MIR168, hvu-MIR5048b
Using the same set of putatively more stable reference genes for miR5048 expression data normalization, miR5048 was down-regulated under drought treatment consistent with the deep sequencing result. [score:6]
The salt treatment did not result in a significant up-regulation of miR5048 expression with either normalizing group (Fig. 5J). [score:6]
In contrast, normalizing the expression level of miR5048 to group 2 candidates resulted in comparatively higher expression of miR5048 with no significant difference between the treated and control samples. [score:5]
Under the fungal infection, miR5048 expression was not abundant and there was no significant difference in expression with treatment detected using either normalizing group of reference genes (Fig. 5H). [score:5]
Under the boron treatment, miR5048 expression normalized to group 1 showed that miR5048 was down-regulated in the treated samples compared to the control samples. [score:5]
We also took advantage of the available boron treated, fungal infected, nitrate treated and salt treated samples to compared miR5048 expression in these conditions normalizing the expression level to the two normalizer groups to check for the groups’ comparative sensitivity. [score:4]
However, normalization with the putatively unstable, commonly used ACT, α-TUB and GAPDH failed to detect statistically significant down-regulation of miR5048 under drought stress, conflicting with our deep sequencing result. [score:4]
Apart from the drought treatment, as there is lack of reference for miR5048 expression, we could not compare our validation results under individual experimental conditions. [score:3]
To evaluate the putatively stable candidates for normalizing miRNA expression, we selected miR5048 for comparison with our pre-existing deep sequencing data for expression of this miRNA in the barley variety ‘Golden Promise’ under drought and well-watered conditions. [score:3]
Comparison of relative expression of (A, B, C, D, E) SUPEROXIDE DISMUTASE (AK363344.1) and (F, G, H, I, J) miR5048 (MIMAT0020544) in drought -treated, boron treated, fungal infected, nitrate treated and salt treated samples and their respective controls by qPCR when normalized to group1 (a combined group of three stable reference genes; snoR14, ADP, snoR23) and group 2 (a combined group commonly used housekeeping genes; ACT, α-TUB, GAPDH). [score:3]
Nonetheless, for miR5048 as well as for SUPEROXIDE DISMUTASE, there was an increase in the sensitivity of detection of differential expression for boron, fungal and salt treatments when our higher-ranked stable candidates were used, once again validating their superiority. [score:3]
Under the nitrate treatment, upon normalization to both normalizer groups, miR5048 was significantly up-regulated compared to the control samples (Fig. 5I). [score:3]
The normalization against group 1 reference genes resulted in down-regulation of miR5048 under drought treatment compared with the control conditions (Fig. 5F) which was consistent with our pre-existing deep sequencing data (see inset in Fig. 5F) [45]. [score:3]
To validate the reliability of putatively more stable candidate reference genes, the relative expression levels of an mRNA (Superoxide dismutase) and an miRNA (miR5048) (S2 Table) were also quantified for all the samples described above by qPCR (as before). [score:3]
We also quantified the relative expression of a barley miRNA, miR5048, normalizing with the same groups of candidates. [score:3]
Expression of miR5048 was compared with previously described deep sequencing data [45]. [score:2]
Melt curve and standard curve of miR5048. [score:1]
Melt curves and standard curves in qPCR for S uperoxide dismutase (AK363344.1) and miR5048 (MIMAT0020544) are given in S5 Fig. and S6 Fig., respectively. [score:1]
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2
[+] score: 17
The targets of miR159 and miR1120 were found to be up-regulated in both root and leaf upon boron stress, but miR395 and miR5048 target genes were down-regulated in root but remained at the same levels in leaf tissue upon boron stress. [score:11]
Although miR168 was induced, miR159, miR396, miR1120 and miR5048 were inhibited in both root and leaf upon excess boron exposure. [score:3]
Both conserved barley miRNAs (miR156, miR159, miR164, miR166, miR168, miR171, miR395 and miR396) and non-conserved barley miRNAs (miR1120 and miR5048) were detected. [score:1]
Contig1_All 10 undetermined hvu-miR5048 RPG1, Serine/threonine protein kinase, NAC domain-containing protein 18 and Serine/threonine kinase-like protein CL26250. [score:1]
Contig7_All Senescence -associated protein or CBL-interacting protein kinase 21 hvu-miR5048 Cellular processMetabolic process – BindingCatalytic activity CL26250. [score:1]
[1 to 20 of 5 sentences]
3
[+] score: 11
Expression of some conserved miRNA families such as miR156 and miR6213 has been detected as upregulated while miR168, miR444, and miR5048 have shown suppressed expression patterns in response to salinity stress in barley (Lv et al. 2012; Deng et al. 2015). [score:10]
durum, T. aestivum Phenyl-alanine tRNA synthetase likeLiu et al. 2015a, Kantar et al. 2011a, Ma et al. 2015 miR5048 T. turgidum ssp. [score:1]
[1 to 20 of 2 sentences]
4
[+] score: 7
Three miRNAs (hvu-miR168-5p, hvu-miR5048a and hvu-miR444b) were down-regulated while two other miRNAs (hvu-miR171-5p and hvu-miR6213) were up-regulated under salinity stress. [score:7]
[1 to 20 of 1 sentences]
5
[+] score: 3
Conversely, miR5071, miR5048 and miR5067 which have only been identified in barley, are expressed at over 100 RPM in the seed. [score:3]
[1 to 20 of 1 sentences]