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18 publications mentioning hvu-MIR156a

Open access articles that are associated with the species Hordeum vulgare and mention the gene name MIR156a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 181
In A. thaliana, two miRNAs, miR156 and miR172, regulated the juvenile to adult developmental phase change [38]; SPL9 and SPL10 promoted the expression of miR172b by binding to its promoter and acted independently of this and its target genes [38]; and the expression of miR156 was higher in the juvenile phase than in the adult phase, whereas the expression of miR172 was lower in the juvenile phase than in the adult phase [38]. [score:11]
Nine HvSPLs (HvSPL1, 3, 6, 11, 13, 15, 16, 17 and 23), including six that are targeted by miR156 (HvSPL3, 11, 13, 16, 17 and 23) were highly expressed and displayed tissue-specific patterns of expression. [score:7]
Interestingly, expression of miR156 targeted HvSPL18 and miR156 non -targeted HvSPL7 and HvSPL20 were unique to INF2 tissue. [score:7]
Liu J Cheng X Liu P Sun J miR156 -targeted SBP-Box transcription factors interact with DWARF53 to regulate TEOSINTE BRANCHED1 and BARREN STALK1 expression in bread wheatPlant Physiol. [score:6]
The miR156 -targeted SPL9 promoted sesquiterpene biosynthesis by binding to the promoter region of TPS21 [26] and it negatively regulated anthocyanin levels by modulating the expression of the MYB-bHLH-WD40 complex [27]. [score:6]
Importantly, tissue-specific differential expression of miR156 -targeted HvSPL genes also suggests that they have possible key role in barley growth and development. [score:6]
Since SPL/miR156 module control panicle branching by directly regulating the miR172/AP2 module in rice 30, 47, bract and ear glume development in maize 48, 49 and floral meristem identity in A. majus 2, 28, expression of HvSPL genes in the mir172 barley mutant was analysed. [score:6]
Splice variant 1 of HvSPL11 (HvSPL11 V1), which contained a miR156 target site, showed lower expression at vegetative and higher at the reproductive phase and was the major transcript (Fig.   3B,C). [score:5]
In Arabidopsis, 10 of the 16 SPL genes are targets of miR156 5, 20 and 11 of the 19 SPL genes in rice have been identified as a targets of miR156 [18]. [score:5]
Expression analyses of HvSPL3, 6, 13, and 23 in our study are aligned with the expression pattern of miR156 and miR172b during vegetative and reproductive phases suggesting the similar role of miR156-HvSPL-miR172b module in growth phase modifications in barley as observed in A. thaliana [38] and maize [61] (Figs  6A,B and S5). [score:5]
However, expression remained constant for variant 2 of HvSPL11 (SPL11 V2), which lacked a miR156 target site and was a minor transcript. [score:5]
The genomic and cDNA sequences of HvSPLs were analysed to predict the putative target sites of miR156 using psRNATarget tool (http://plantgrn. [score:5]
Earlier studies in A. thaliana indicated that floral transition was regulated by gibberellin guided miR156 -targeted SQUAMOSA PROMOTER BINDING-LIKE transcription factors [58]. [score:4]
Conserved Motif Identification, Cis-Regulatory Elements, miR156 Target Site Prediction and Alternative Splicing Event Analysis. [score:4]
Conserved Motif Identification, Cis-Regulatory Elements, miR156 Target Site Prediction and Alternative Splicing Event AnalysisA search for conserved motifs within HvSPL proteins was performed by using the MEME 4.11.0 tool (http://meme-suite. [score:4]
Yu Z-X Progressive regulation of sesquiterpene biosynthesis in Arabidopsis and Patchouli (Pogostemon cablin) by the miR156 -targeted SPL transcription factorsMol. [score:4]
Vegetative to Reproductive Phase in Barley: Expression of miR156, miR172 and Specific SPL GenesThe timing of juvenile to adult phase transition in A. thaliana is known to be regulated by miR156 and miR172, along with several members of the SPL family [38]. [score:4]
Most of the splice variants of miR156 -targeted HvSPLs exhibited miR156 complementary sites, implying the existence of alternate splicing -mediated regulation of biological processes in barley (Fig.   3, Table  S7). [score:4]
The expression pattern of miR156 family members and miR172b in barley vegetative to reproductive phase was also antagonistically related (Fig.   5A–C). [score:3]
Vegetative to Reproductive Phase in Barley: Expression of miR156, miR172 and Specific SPL Genes. [score:3]
The miR156 complementary sites are present in the coding region or in the 3′ un-translated region (3′-UTR). [score:3]
HvSPL genes that contain miR156 target sites are indicated by (*) asterisks. [score:3]
The miR156 target sites with the nucleotide positions of HvSPL transcripts are shown in green. [score:3]
Xu M Developmental functions of miR156-regulated SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes in Arabidopsis thalianaPLoS Genet. [score:3]
In A. thaliana, 10 of 17 SPL genes are targeted by miR156, suggesting that miR156 complementary sites in SPL genes are conserved across plant species. [score:3]
Therefore, miR156 family in barley genome and its target site in HvSPLs was studied. [score:3]
Interestingly, differences in the miR156 target site among splice variants were also observed. [score:3]
Similarly, miR156 non -targeted (that is, lacking a miR156 binding site) HvSPLs also generated splice variants (1 to 20 numbers) of varying length. [score:3]
As expected, expression of miR156 family members was higher in 11-d-old seedlings stage (vegetative phase) and lower in 70–75 days old plants (reproductive phase). [score:3]
Data are expressed as RPM (reads per million) for the miR172 and miR156 members normalized to all miRNAs identified in the sample. [score:3]
Green colour denotes the mature sequence of miR156a/b/c and d. (C) miR156 target site in HvSPL3, 11, 13, 16, 17, 18 & 23 genes. [score:3]
We identified 4 members of miR156 family in barley and target prediction showed that 7 of the 17 SPL genes contained a complementary site for this miRNA (Fig.   2A–C; Table  S5). [score:3]
In case of HvSPL3 (20 splice variants), HvSPL11 (4 splice variants) and HvSPL17 (14 splice variants), only 15, 1 and 9 number of splice variants contained miR156 target site. [score:3]
Expression Analysis of Barley miR156 and miR172 Family Members. [score:3]
In addition, the expression patterns of SPL genes and of miR156 and miR172 from vegetative to reproductive phases revealed their possible functional relationships. [score:3]
Antagonistic expression pattern of miR156 and miR172b was observed during vegetative and reproductive phases of barley. [score:3]
Figure 5Barley miR172 sequences and expression analysis of miR156 and miR172 family members. [score:3]
The results of the current study revealed that the miR156/HvSPL/miR172 module functions as key molecular integrators that affected developmental phase transitions and spike development in barley. [score:3]
Putative miR156 binding sites were found for HvSPL3, HvSPL11, HvSPL16, HvSPL17, HvSPL18 and HvSPL23 in their coding regions and for HvSPL13 in the 3′UTR (Fig. 2C; Table  S5), suggesting that regulation by miR156 is restricted to this subset of HvSPL genes. [score:2]
Gou J The miR156-SPL4 module predominantly regulates aerial axillary bud formation and controls shoot architectureNew Phytol. [score:2]
Xie K Wu C Xiong L Genomic organization, differential expression, and interaction of SQUAMOSA promoter -binding-like transcription factors and microRNA156 in ricePlant Physiol. [score:2]
In bread wheat, it was found that the miR156-SPL module regulated bread wheat plant architecture by interacting with a strigolactone signalling repressor gene, DWARF53 [34]. [score:2]
MiR156 Family in H. vulgare and Their Target Site in HvSPL Genes. [score:2]
In A. thaliana, these phases are regulated by miR156 and miR172 [38] via SPL genes. [score:2]
The present study represents the first comprehensive analysis of the miR156/SPL/miR172 regulatory hub in barley. [score:2]
The timing of juvenile to adult phase transition in A. thaliana is known to be regulated by miR156 and miR172, along with several members of the SPL family [38]. [score:2]
Genetic modification of the miR156-SPL4 module controls aerial axillary bud formation, branching, biomass yield, and re-growth after cutting in switchgrass [36]. [score:1]
Two putative members of miR156 family, Hv-miR156a (accession number MI0016449) and Hv-miR156b (accession number MI0030546) were identified for barley in the miRbase database (http://www. [score:1]
The mature miR156 sequences of all four members were identical, but divergence was observed in the precursor sequences which showed 71 to 87% homology. [score:1]
All HvSPLs with miR156 binding sites were predicted to produce splice variants (4 to 20 numbers). [score:1]
* Asterisks denote the presence of the miR156 complementary sequence in the splice variants of barley SPL11 gene. [score:1]
The miR156-complementary site was present in coding regions of HvSPL3, 11, 16, 17, 18, 23, and in the 3′UTR of HvSPL13. [score:1]
The respective transcripts of miR156 and miR172 has been shown in RPM (reads per million). [score:1]
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[+] score: 94
Other miRNAs from this paper: hvu-MIR168, hvu-MIR171, hvu-MIR156b
As expected, HvSPL was down-regulated in OE171, reinforcing the hypothesis that miR171 acts at least partially through the up-regulation of miR156. [score:7]
Additionally, AP2 was recently shown to directly activate MIR156e expression in Arabidopsis [38], suggesting that the up-regulation of HvAP2L in OE171 could be responsible for the increasing abundance of miR156. [score:7]
In grass plants, over -expression of miR156 promotes vegetative branching producing an increased number of tillers but inhibits inflorescence branching resulting in a reduced number of spikelets [2- 5, 9]. [score:5]
Secondly, miR171 could repress vegetative phase transitions in barley through the upregulation of miR156, a known regulator of the transition from juvenile to adult phases across the angiosperms [2, 6- 9, 13, 45]. [score:5]
In Arabidopsis miR156 down-regulates AtSPL9/10 which in turn directly activates the transcription of MIR172b[13]. [score:5]
Using the same tissues, we quantified the relative abundance of two barley mRNAs, coding for an SPL gene predicted to be targeted by miR156, HvSPL (U21_18637) and a potential target of miR172, HvAP2L (U21_18652) (Figure 5A). [score:5]
In addition, the data show that miR171 over -expression alters the vegetative to reproductive phase transition by activating the miR156 pathway and repressing the expression of the TRD (THIRD OUTER GLUME) and HvPLA1 (Plastochron1) genes. [score:5]
In Arabidopsis, rice and maize, miR156 regulates shoot branching, leaf initiation and juvenile-to-adult phase transition through the down-regulation of several SPL genes [2- 9]. [score:5]
During vegetative shoot development in Arabidopsis and maize, miR156 expression gradually decreases and is inversely correlated with that of miR172 [2, 12, 13]. [score:4]
It is proposed that miR171 acts up-stream of miR156 and TRD which in turn may regulate Hv PLA1 expression as was observed for their orthologs in rice [44]. [score:4]
The effect of OE171 on miR156 and miR172 expression was examined using inflorescence tissue from T [1] OE171 and WT plants grown under LD conditions (Figure 5B). [score:3]
Detailed analysis of miR156 function in the maize inflorescence has shown that it has a role in establishing axillary meristem boundaries by spatially restricting expression of the SPL gene Tasselsheath4 (TSH4) to the bract. [score:3]
The regulators of these transitions include miRNAs, in particular miR156 and miR172 which are part of a regulatory module conserved across the angiosperms. [score:3]
In agreement with this hypothesis, a decrease in miR172 expression was detected in the young leaves of OE171 T [0] plants which over-accumulated miR156 (Figure 2B). [score:3]
miR156 abundance was higher in OE171 lines but no change in miR172 expression was detected. [score:3]
Over -expression of miR156 leads to a decrease in miR172 abundance in young shoots [2, 13]. [score:3]
In addition a delay in the transition to reproductive growth involving miR156 was observed which suggests that there are monocot specific functions for miR171 and its target genes. [score:3]
The authors proposed that miR156 principally affects miR172 during the early vegetative stage by repressing MIR172b expression and that the increasing miR172 abundance during the late vegetative stage is driven by other MIR172 genes independent of miR156. [score:3]
Our data suggest that some of the roles of miR171 and its target genes that have been determined in Arabidopsis are conserved in barley and that they have additional functions in barley including activation of the miR156 pathway. [score:3]
The miRNAs with the best-defined roles in regulating phase changes in the shoot meristem are miR156 and miR172 which form a regulatory module that is wi dely conserved in plants. [score:3]
The AtSPL3/4/5 genes are regulated both transcriptionally and post-transcriptionally by miR172 [14] and miR156, [15], respectively. [score:2]
In maize, miR156 controls plant architecture by regulating at least 2 SPL genes, Teosinte Glume Architecture1 (TGA1) and Tasselsheath4 (TSH4)[10, 12]. [score:2]
Thirdly, miR171 promotes vegetative traits in barley through a secondary pathway, independent from miR156 [40], that involves TRD and HvPLA1[42, 43]. [score:1]
Interestingly, OE171 and OE156 in Arabidopsis show opposite effects on leaf initiation [30, 32, 40], suggesting that the possible connection between the miR171 and miR156 pathways may be monocotyledon specific. [score:1]
Recent studies have demonstrated cross talk between the miR156 and miR172 pathways, which appear to have conserved roles in coordinating the timing of vegetative phase changes and competency to flower in all angiosperms [11]. [score:1]
Maize OE156 plants did not show the same flowering defect as for loss of miR172 function [2, 12], suggesting that the function of miR172 in the inflorescence is independent of miR156. [score:1]
In Arabidopsis the leaf initiation rate is likely to be controlled by two independent pathways, one involving miR156- SPLs and the other involving two orthologs of the rice PLASTOCHRON1 (PLA1) gene which encode cytochrome P450 enzymes [39, 40]. [score:1]
The increased accumulation of miR156 in OE171 did not cause a reduction of miR172 abundance in contrast with previous observations in Arabidopsis and maize [2, 13]. [score:1]
This correlates with the observation that miR172 abundance continues to increase during later vegetative stages while the miR156 level stays at a constant minimum level [13, 14]. [score:1]
These phenotypic similarities suggest that miR171 is linked to the miR156-miR172 pathway in barley. [score:1]
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3
[+] score: 59
Most of these targets are annotated as transcription factor-coding genes, including SQUAMOSA promoter -binding protein-like genes (SPLs) targeted by miR156, MYB transcription factors (MYBs) targeted by miR159, auxin response factors (ARFs) targeted by miR160, and TCP family transcription factors (TCPs) targeted by miR319. [score:11]
As shown in Fig. 4b, miR156 and miR167 were expressed highly at the three developmental stages we tested; miR164 was expressed mainly at the seed development and postgermination stages; miR393, miR172, and miR396 were expressed preferentially in embryo at 5 DAG. [score:9]
Regulation of OsSPL14 by OsmiR156 defines the ideal plant architecture in rice, whereas OsSPL16, another target of miR156, is involved in a regulatory module determining grain shape and can be modulated to improve rice yield and grain quality [43, 44]. [score:5]
In Arabidopsis, miR156 regulates the juvenile-to-adult phase and floral transition through targeting SPLs [37– 39]. [score:4]
Notably, we found that miR5565 and miR2199 were significantly and highly expressed in barley embryos during seed germination, whereas miR156, miR166, miR167, and miR168 accumulated in both the seed development and germination stages (Fig. 2). [score:4]
In this study, we showed that miR156 exhibited a dynamic expression pattern during seed development and germination. [score:4]
According to bioinformatics analysis, we found that miR156 was the most highly expressed miRNA in embryos of developing and germinating seeds. [score:3]
Through analysis of degradome sequencing data, three SPL gene family members including AK356077, AK374598 and MLOC_11199 were predicted to be the target genes of miR156 (Table 1). [score:3]
In rice and maize, miR156, together with miR172, acts as a regulator of inflorescence and tiller development [40– 42]. [score:3]
Conserved miRNA families such as miR156, miR168, miR166, miR167, and miR894 were highly expressed in embryos of developing and germinating seeds. [score:3]
Among them, miR156, miR159, miR390, miR164, miR396, and miR319 were predicted to regulate the ethylene pathway, miR159 regulates GA signalling; and miR172, miR396, and miR319 are likely to be associated with cytokinin signalling. [score:3]
This indicates that miR156 and miR166 -mediated repression of transcriptional factors might be conserved mechanisms to modulate cell differentiation during seed development and germination. [score:2]
Among them, four miRNAs including miR156, miR167, miR166, and miR894 are conserved in all plant species registered in miRBase; three miRNAs (miR168, miR2118, and miR164) are conserved among angiosperm; six miRNAs (miR2916, miR6441, miR2199, miR7696, miR8124, and miR6300) are detected only in dicotyledons; and five miRNAs (miR5565, miR1869, miR5813, miR5071, and miR5060) seem to be conserved families across grass families. [score:1]
miR396 and miR156 increased their abundance when seeds entered the maturation stage. [score:1]
miR156 is reported to be one of the most abundant miRNAs conserved among nearly all land plants including mosses [36]. [score:1]
During the early seedling growth stage, miR156, miR396, miR167, and miR164 increased their abundance in root, shoot, and leaf tissues, especially at 10 DAG. [score:1]
miR156 preferentially accumulated in embryos at 15 DPA and 25 DPA (Fig. 4c) and displayed an increasing trend in the early seedling growth stage (Figs. 4d and 5). [score:1]
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4
[+] score: 48
In their study, they found that the expression of miR164, miR395, and miR156 was downregulated while miR159, miR167, and miR171 expression was upregulated in leaf tissues of wheat. [score:11]
Expression of some conserved miRNA families such as miR156 and miR6213 has been detected as upregulated while miR168, miR444, and miR5048 have shown suppressed expression patterns in response to salinity stress in barley (Lv et al. 2012; Deng et al. 2015). [score:10]
Downregulation of miR156, miR159, miR164, miR398, and miR408 was observed under Cd stress while their targets were mostly upregulated. [score:9]
The miR156-SPL module is associated with heat stress response and memory to delay flowering by the repressing of the expression of SPL TFs in Arabidopsis (Stief et al., 2014) and also the regulation of lateral root development which determines the efficiency of water and nutrient uptake in Brassica (Yu et al. 2015). [score:5]
SPL was detected in the modulation of transition from juvenile phase to adult phase in the shoot development process in Arabidopsis, where miR156 regulates the expression of miR172 through several members of this TF (Wu et al. 2009a, b). [score:5]
Many conserved miRNAs across monocots and dicots, such as miR156, miR159, or miR164, have been shown to target stress -associated transcription factors such as MYB and NAC family members (Gupta et al. 2014; Qiu et al. 2016). [score:3]
Abundance of AGO5 -associated isomiRs of miR156, miR158, and miR845 in the pollen and sperm, combined with the findings described above, suggests a novel and intricate miRNA -mediated regulation mechanism for reproductive cells relative to somatic cells (Borges et al. 2011). [score:2]
Also, the miR156-SPL association was shown to be effective in grain development of rice and barley (Miura et al. 2010; Curaba et al. 2012). [score:2]
dicocoides Mn superoxide dismutaseKantar et al. 2011a miR156 T. turgidum ssp. [score:1]
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5
[+] score: 32
Expression of miR156 has been investigated in many studies as down-regulated in Oryza sativa, Zea mays, Populus tremula, Populus trichocarpa in response to drought stress, salt stress, cold stress, mechanical stress, while up-regulated in Arabidopsis thaliana, Triticum aestivum, Nicotiana tabacum upon salt stress, heat stress, viral infection, respectively [59]. [score:7]
miR156, miR169c, miR171, miR171a, miR444a, miR444c, miR2023a were up-regulated while miR156d, miR397, miR408, miR1121, miR2014, miR5049, miR5141, miR5180, and miR5180a were down-regulated in leaf tissue upon boron stress. [score:7]
In addition, the target of miR156a, SBP protein gene, was down-regulated in stressed leaves, but was unaltered in roots in response to boron stress (Fig. 2). [score:6]
In addition, some miRNAs such as miR156, miR169, miR172, and miR1121 were highly expressed in root but miR2004 was highly expressed in leaf. [score:5]
Our study demonstrated that boron stress inhibited miR156a expression in barley leaves. [score:5]
Both conserved barley miRNAs (miR156, miR159, miR164, miR166, miR168, miR171, miR395 and miR396) and non-conserved barley miRNAs (miR1120 and miR5048) were detected. [score:1]
Our study indicates that miR156 was also boron stress responsive in leaves upon excess boron treatment. [score:1]
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6
[+] score: 23
Other miRNAs from this paper: hvu-MIR168, hvu-MIR444a, hvu-MIR156b
Therefore, miR156 suppresses the expression of miR172. [score:5]
The most abundant miR156 was over two-fold up-regulated in transgenic barley. [score:4]
Previous studies showed that miR156 targets SQUAMOSA promoter binding protein-like (SPL) TFs, which control flowering time, phase change, leaf initiation rate and positively regulate miR172 [27]– [28]. [score:4]
This suggests that the regulation of miR156 in vivo may be true and miR156 is a good candidate for drought tolerance and for further functional analysis of the impact of TaDREB3 on the expression of miRNAs. [score:4]
doi:10.1007/s10142-010-0181-4. 27 Wang JW, Czech B, Weigel D (2009) miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. [score:2]
It is to note that miR172 can in turn positively regulate the SPL TFs, but not miR156 [28]. [score:2]
Coincidently, miR156 is induced by drought [26] and TaDREB3 is specially functional in drought tolerance and induced by drought [14]. [score:1]
hvu-miR156 was the most abundant miRNA in transgenic barley, accounting for 52.4% of the total reads, and the second most abundant in non-transgenic barley, while hvu-miR168-5p was the most abundant in non-transgenic barley, accounting for 71.3% of the total reads, and the second most abundant miRNA in transgenic barley (Table 4). [score:1]
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7
[+] score: 17
Sometimes different targets for a specific miRNA are members of the same gene family (e. g. miR156-SBP family), while in other cases there is no evident relationship among the putative targets of a given miRNA (e. g. miR1121). [score:5]
The screening of barley databases has identified two SBP genes targeted by miR156 for which two nucleotide variations occur in critical positions (11-12). [score:3]
The Squamosa-promoter Binding Protein (SBP) is a known target family for miR156. [score:3]
In rice Zhou et al. have found a high number of targets for miR156 and miR396 and a low number for miR162, miR167, miR395, miR398 and miR399 [12]. [score:3]
miR156 performs a critical function in mediating developmental processes and it is also related to the response to biotic stress. [score:2]
The increase of the activity of some miRNAs (among which miR156) is part of the infection strategy performed by the Turnip mosaic virus in Arabidopsis [26, 27]. [score:1]
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8
[+] score: 12
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR398a, osa-MIR398b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, osa-MIR396e, zma-MIR396b, zma-MIR396a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR156k, zma-MIR160f, tae-MIR159a, tae-MIR159b, tae-MIR160, tae-MIR164, tae-MIR167a, tae-MIR1127a, osa-MIR169r, osa-MIR396f, zma-MIR396c, zma-MIR396d, osa-MIR2275a, osa-MIR2275b, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, osa-MIR396g, osa-MIR396h, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, tae-MIR156, hvu-MIR159b, hvu-MIR159a, hvu-MIR166a, tae-MIR167b, hvu-MIR168, hvu-MIR169, tae-MIR169, hvu-MIR397a, tae-MIR398, tae-MIR171b, hvu-MIR166b, hvu-MIR166c, osa-MIR2275c, osa-MIR2275d, tae-MIR1122b, tae-MIR9653a, tae-MIR9654a, tae-MIR9656, tae-MIR9657a, tae-MIR9659, tae-MIR9660, tae-MIR1127b, tae-MIR9661, tae-MIR396, tae-MIR9665, tae-MIR2275, tae-MIR9667, tae-MIR167c, tae-MIR1120b, tae-MIR397, tae-MIR1130b, tae-MIR5384, tae-MIR9675, tae-MIR1120c, tae-MIR9679, tae-MIR9657b, hvu-MIR397b, hvu-MIR156b, tae-MIR9653b
miR156 targets squamosa promoter -binding protein-like 10 (SPL10) and SPL11, and the regulation of these targets prevents premature gene expression during early embryogenesis [19]. [score:8]
Of the 15 known miRNA families, 8 (miR396, miR168, miR156, miR172, miR159, miR398, miR1318 and miR167) showed different levels of preferential expression in wheat flag leaves, with the logarithm of the fold changes ranged from 0.5 to 5.2 as well as more than those in the developing seeds (Figure  3a, Table  2). [score:3]
The highest read abundance (approximately 238,000 RPM) was detected in the miR168 family and was 3.8 to 78 times more abundant than the other miRNA families, including miR156, miR166, miR167 and miR172, whose abundance ranged from about 2,900 RPM to 62,000 RPM (Table  2). [score:1]
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9
[+] score: 9
We validated 11 conserved targets of six known miRNA families which code for transcription factors known to control key steps in plant development: miR156- SPL (2 genes), miR159- Myb, miR164- NAC, miR167- ARF (potentially 3 genes sharing the same degradome peak), miR169- CBF (3 genes) and miR172- AP2like (Table 5). [score:4]
miR156 controls shoot branching in rice and maize [33- 35] and miR172 regulates floral organ identity in rice, maize and barley [36- 41]. [score:2]
In maize, miR172 accumulation is affected by miR156 and both miRNAs are involved in the regulation of the juvenile to adult phase transition [33]. [score:2]
The pot-miRNAs identified in this analysis included many homologs of known miRNA families that varied in sequence and length to previously identified sequences (e. g. 71 miR156 homologs and 24 miR168 homologs). [score:1]
[1 to 20 of 4 sentences]
10
[+] score: 8
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR171a, osa-MIR393a, osa-MIR397a, osa-MIR397b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319b, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR168b, osa-MIR169f, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR172d, osa-MIR171i, osa-MIR166m, osa-MIR166j, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319b, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169f, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, osa-MIR529a, tae-MIR159a, tae-MIR159b, tae-MIR171a, tae-MIR1120a, osa-MIR1430, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR166n, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR397a, zma-MIR397b, tae-MIR156, hvu-MIR159b, hvu-MIR159a, hvu-MIR166a, hvu-MIR168, hvu-MIR171, hvu-MIR397a, tae-MIR171b, hvu-MIR1120, hvu-MIR166b, osa-MIR3981, hvu-MIR166c, tae-MIR1120b, tae-MIR397, tae-MIR1120c, hvu-MIR397b, hvu-MIR156b
Both 20 and 21 nt long miR156 were expressed at the highest level in 68-day-old plants. [score:3]
The 20 nt long mature miR156 was previously identified in barley using deep sequencing [48]. [score:1]
Hybridization also revealed the presence of two mature miR156, 20 and 21 nt long (Figure 6H). [score:1]
A 21 nt long mature miR156 with an additional adenosine residue at the 3 [′] end is annotated in the databases of many eukaryotic species [50, 51]. [score:1]
Based on nucleotide sequence and structural similarities, we classify barley MIR156 as an orthologue of rice MIR156g (Figure 6B). [score:1]
Both the 20 and 21 nt miR156 species were equally represented in 6-week- and 68-day-old plants; however, in 1- and 2-week-old plants, primarily the 20 nt long miR156 was detectable. [score:1]
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11
[+] score: 8
Other miRNAs from this paper: hvu-MIR156b
2, and miR156 level is shown as a loading control. [score:1]
2, and miR156 and 5S rRNA are served as loading controls. [score:1]
2, and the miR156 and 5S rRNA are served as loading controls (panel b). [score:1]
The levels of miR9863a and phasiRNAs were detected by RNA gel-blot using probes for miR9863a and siRNAs (see supplemental Table 2), and miR156 and 5S rRNA are shown as loading controls (panel a). [score:1]
2 and miR156a signal, respectively; probe J87 was used for Mla1 derived phasiRNAs in S8 Figure according to Chen and colleagues [44]. [score:1]
1. miR156 and 5S rRNA were used as controls (panel b). [score:1]
The level of miR9863 members was analyzed by RNA gel blot using miR156 as a loading control (panel a), or by qRT-PCR normalized against U6 (panel b). [score:1]
miR156 and 5S rRNA were employed as loading controls. [score:1]
[1 to 20 of 8 sentences]
12
[+] score: 5
According to qRT-PCR results, miRNA156 exhibited higher level of expression in root, young leaves and spikes, but lower in mature leaves and spikes. [score:3]
The miR156 was the largest family with 10 members, followed by miR166 and miR169 each with 9 members, and miR396 with 8 members. [score:1]
The miR156 family is a large miRNA family playing important roles in various biological and metabolic processes [27]. [score:1]
[1 to 20 of 3 sentences]
13
[+] score: 4
Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156 -targeted SPL transcription factor. [score:4]
[1 to 20 of 1 sentences]
14
[+] score: 4
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR162a, osa-MIR164a, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR394, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR398a, osa-MIR398b, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, osa-MIR156k, osa-MIR156l, osa-MIR159b, osa-MIR162b, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR408, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR437, osa-MIR396e, osa-MIR444a, osa-MIR528, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR529b, tae-MIR159b, tae-MIR167a, tae-MIR399, tae-MIR408, tae-MIR444a, osa-MIR1432, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR1848, osa-MIR1858a, osa-MIR1858b, osa-MIR1862a, osa-MIR1862b, osa-MIR1862c, osa-MIR1871, osa-MIR1862d, osa-MIR1862e, osa-MIR827, osa-MIR396f, osa-MIR396g, osa-MIR396h, osa-MIR396d, osa-MIR395x, osa-MIR395y, tae-MIR156, hvu-MIR159b, hvu-MIR166a, tae-MIR167b, hvu-MIR168, tae-MIR395a, tae-MIR395b, hvu-MIR397a, tae-MIR398, tae-MIR444b, hvu-MIR166b, hvu-MIR444a, osa-MIR1862f, osa-MIR1862g, hvu-MIR399, hvu-MIR444b, hvu-MIR166c, tae-MIR396, tae-MIR167c, tae-MIR397, hvu-MIR397b, hvu-MIR156b
Recent studies showed that miR172 acts downstream of miR156 and is regulated by miR156 [56]. [score:2]
Interestingly, miR156 is the second most abundant miRNA in the barley dataset, accounting for about 3.7% of the total reads (Additional file 1). [score:1]
These results, combined with the fact that Brachypodium is closer to barley than to rice [57, 58], lead us to speculate that miR156 may have different or additional roles in barley and Brachypodium relative to rice. [score:1]
[1 to 20 of 3 sentences]
15
[+] score: 4
While some salt related miRNA were identified to show a different expression profile under salinity stress among different plants, such as miR156, miR169 and miR396 [8, 11, 12], which indicated that there might be some species-specific response or tolerance mechanism in miRNA-meditated gene regulation for various plants under salinity stress [13]. [score:4]
[1 to 20 of 1 sentences]
16
[+] score: 2
Very recently, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 15 (AtSPL) was demonstrated to be regulated by miR156 and to promote flowering under non-inductive conditions (Hyun et al., 2016). [score:2]
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17
[+] score: 2
Because miR156 and miR172 participate in the age -dependent regulation of flowering in diverse plants 12 13, it will be interesting to explore whether alterations in FT2 growth-related AS in B. distachyon is controlled by these two miRNAs during flowering processes. [score:2]
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[+] score: 1
In Arabidopsis, the LFY, FUL and AP1 genes are activated by SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 (SPL3) (Figure 1) which is regulated by FT and microRNA156 [100]. [score:1]
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