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10 publications mentioning oar-mir-21

Open access articles that are associated with the species Ovis aries and mention the gene name mir-21. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 105
miR-328 was found downregulated in goats but upregulated in sheep; two miRNAs—miR-2285f-2 and miR-27a-5p were found upregulated in goats but downregulated in sheep; six miRNAs—miR-320a-1, miR-320a-2, miR-1246, miR-363, miR-760-3p, and miR-21-3p were upregulated and two miRNAs—miR-34b and miR-150 were downregulated in both species (Figure 1B and Table 3). [score:19]
In PPRV infected sheep lung, the upregulated miRNAs—miR-21-3p and miR-320a govern immune genes—TRAF6, EGFR, and ERBB4 and the downregulated miR-27a-5p potentially modulates the expression of genes—MAP3K7 and MAPK8IP3, involved in JNK signaling pathways (Figure 3B). [score:9]
miRNAs Role Tissue Reference miR-27a-5p Repression of viral replication Lung Roberts et al., 2011b miR-21-3p Induce apoptosis Lung Lo et al., 2013 miR-320a Inhibit virus infection Lung Sun et al., 2014 miR-1246 Promotes virus cytotoxicity Lung Sheng et al., 2014 miR-363 Induced apoptosis Lung and spleen Zhang et al., 2014 miR-760-3p Very highly upregulated Lung and spleen – FIGURE 2 Overview of the miRNA prediction and identification of miRNAs that regulated dysregulated proteins. [score:8]
miRNAs Role Tissue Reference miR-27a-5p Repression of viral replication Lung Roberts et al., 2011b miR-21-3p Induce apoptosis Lung Lo et al., 2013 miR-320a Inhibit virus infection Lung Sun et al., 2014 miR-1246 Promotes virus cytotoxicity Lung Sheng et al., 2014 miR-363 Induced apoptosis Lung and spleen Zhang et al., 2014 miR-760-3p Very highly upregulated Lung and spleen – FIGURE 2 Overview of the miRNA prediction and identification of miRNAs that regulated dysregulated proteins. [score:8]
Of these 20 common DEmiRNAs, miR-21-5p was the most upregulated (log [2]FC = 2.35) and miR-199b was the most downregulated DEmiRNA (log [2]FC = -3.03) in the spleen of goats. [score:7]
MicroRNA-21-3p, a berberine -induced miRNA, directly down-regulates human methionine adenosyltransferases 2A and 2B and inhibits hepatoma cell growth. [score:7]
Among these 31 DEmiRNAs, six DEmiRNAsmiR-21-3p, miR-320a, miR-27a-5p, miR-1246 (expressed in lung of both species), miR-760-3p and miR-363 (expressed in lung and spleen of both species) were selected based on their role in viral infection, apoptosis and fold change. [score:5]
These miRNAs include miR-21-3p, miR-320a, miR-27a-5p, and miR-1246—expressed in lung of both species; miR-760-3p and miR-363—expressed in lung and spleen of both species (Figure 2). [score:5]
However, in infected lung miR-21-3p was found upregulated on qPCR though not found in small RNA sequencing data in both the species (Figure 5 and Table 5). [score:4]
The upregulation of miR-21-3p and miR-363 in PPRV infections suggests synergistic effect of these miRs along with the virus in inducing apoptosis. [score:4]
This suggests that PPRV -induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis in the lung and spleen of sheep by downregulating several immune response genes. [score:4]
To further validate the expression of DEmiRNAs from high-throughput sequencing, qPCR was performed on three DEmiRNAsmiR-21-3p, miR-363, and miR-760-3p. [score:3]
Among these 11 common DEmiRNAs, the expression of miR-21-3p, miR-760-3p, and miR-27a-5p was more abundant in lungs of PPRV infected goats with log [2] fold change of 5.82, 3.79, and 3.07, respectively, while miR-34b (log [2]FC = -2.53) and miR-2285f-2 (log [2]FC = -2.53) were found least abundant in lungs of goats and sheep, respectively. [score:3]
Lung (goats)Lung (sheep)Spleen (goats)Spleen (sheep) miRNAsqPCR (log [2] fold changemiRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change) miR-21-3p +6.619 +5.826 +4.344 +1.753 +0.189 – +0.097 – miR-363 +5.823 +2.425 +0.345 +1.049 +0.618 +1.555 +0.474 +1.286 miR-760-3p +5.433 +3.798 +0.629 +1.242 +2.199 +1.637 +1.784 +1.679 PPR is a major threat to livestock keepers in developing countries, causing a severe disease in goats and sheep. [score:3]
The expression of miR-21-3p was found to be in concordance with the sequencing results in PPRV infected spleen of both the species. [score:3]
PPRV infection in spleen and lung triggered the expression of many immune-related miRNAs, including, miR-21, miR-150, miR-146b, and let-7 family as reported in Japanese encephalitis virus infection (Cai et al., 2015). [score:3]
TRAF6, a major element in IFN production (Yoshida et al., 2008) was suppressed by PPRV -induced miR-21-3p and miR-320a in the lung of sheep. [score:3]
In the miRNA–protein network of lung tissue of goats, three miRNAs—miR-21-3p, miR-363, and miR-320a mutually regulate EGFR (epidermal growth factor receptor), which is involved in immune response. [score:2]
The TRIM (tripartite motif family) family members TRIM24, TRIM36, and TRIM45 were identified to be modulated by miR-1246, miR-320a, and miR-21-3p, respectively. [score:1]
The change in the miRNA expression of miR-21-3p, miR-363, and miR-760-3p was calculated with U6snRNA as reference gene for normalization. [score:1]
Lung (goats)Lung (sheep)Spleen (goats)Spleen (sheep) miRNAsqPCR (log [2] fold changemiRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change)qPCR (log [2] fold change)miRNA-seq (log [2] fold change) miR-21-3p +6.619 +5.826 +4.344 +1.753 +0.189 – +0.097 – miR-363 +5.823 +2.425 +0.345 +1.049 +0.618 +1.555 +0.474 +1.286 miR-760-3p +5.433 +3.798 +0.629 +1.242 +2.199 +1.637 +1.784 +1.679 Viral infection in the lung and spleen of sheep and goats infected with PPRV was confirmed by RT-PCR of 351 bp N gene amplicon in lung and spleen (Supplementary Figure S1). [score:1]
miR-21-3p induce apoptosis (Lo et al., 2013) and PPRV is also reported to cause apoptosis of host cells (Mondal et al., 2001). [score:1]
This study revealed that PPRV -induced miR-21-3p, miR-320a, and miR-363 might act cooperatively to enhance viral pathogenesis, which warrants further research. [score:1]
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2
[+] score: 25
Other miRNAs from this paper: oar-mir-134, oar-mir-26b, oar-mir-27a
In particular, microRNA-21 (miR-21) was found to be up-regulated in cervical cancer tissues [13] Accumulating evidence for differential expression of miR-21 in cervical cancer suggests that it might play a crucial role in tumor biology [15]. [score:6]
Conversely, negative feedback regulation of TLR4/NF-kB signaling may be reached by miR-21 induction after C. parvum infection, as miR-21 targets PDCD4, a proinflammatory protein that promotes activation of NF-kB and suppresses interleukin 10. [score:6]
In parasite disease, it had been found that inhibition of miR-21 resulted in increased Cryptosporidium parvum burden [28]. [score:5]
Among the known differential expression of miRNAs, miR-21-3p, miR-542-5p, miR-671, miR-134-5p, and miR-26b, miR-27a showed a much higher expression in CE-R sheep compared to the CE-NR library, in terms of normalized read counts (fold changes were 3.37, 3.5, 6.88, 5.25, 3.75, and 3.2, respectively). [score:4]
In the present study, significant overexpression of miR-21 was obtained in CE-resistant sheep, which suggested that it might be a biomarker associated with CE resistance. [score:3]
It has been confirmed that miR-21 was transactivated through promoter binding of NFKB-P65. [score:1]
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3
[+] score: 8
The other highly expressed miRNAs, such as oar-let-7a, oar-miR-21 and oar-miR-125b have been reported to be highly expressed in ovine ovarian follicles [2]. [score:5]
In previous studies, miR-21 was identified to be able to promote follicular cell survival during ovulation [29], miR-143 was critical for the formation of mouse primordial follicles [30] and let-7b was shown to be necessary for normal development of the corpus luteum [10]. [score:2]
Some of the abundant miRNAs in the anestrous ovary of Tan sheep, including miR-143, miR-26a, let-7 and miR-21, were also reported to be highly abundant in ovaries of human, cow [23– 25], pig [26], adult and neonatal mouse [27, 28] and sheep [2, 3]. [score:1]
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4
[+] score: 8
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181a-1, hsa-mir-215, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-mir-15b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-141, hsa-mir-143, hsa-mir-152, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-184, hsa-mir-200c, hsa-mir-155, hsa-mir-29c, hsa-mir-200a, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-342, hsa-mir-148b, hsa-mir-451a, ssc-mir-125b-2, ssc-mir-148a, ssc-mir-15b, ssc-mir-184, ssc-mir-224, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-let-7f-1, ssc-mir-103-1, ssc-mir-21, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-671, hsa-mir-378d-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-31, bta-mir-15b, bta-mir-215, bta-mir-30e, bta-mir-148b, bta-mir-192, bta-mir-200a, bta-mir-200c, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-mir-342, bta-let-7f-1, bta-let-7a-2, bta-let-7a-3, bta-mir-103-2, bta-mir-125b-2, bta-mir-15a, bta-mir-99b, hsa-mir-664a, ssc-mir-99b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-16-2, ssc-mir-16-1, bta-mir-141, bta-mir-143, bta-mir-146a, bta-mir-152, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-223, bta-mir-224, bta-mir-26a-1, bta-mir-296, bta-mir-29d, bta-mir-378-1, bta-mir-451, bta-mir-486, bta-mir-671, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, ssc-mir-181a-1, ssc-mir-215, ssc-mir-30a, bta-mir-2318, bta-mir-2339, bta-mir-2430, bta-mir-664a, bta-mir-378-2, ssc-let-7a-1, ssc-mir-378-1, ssc-mir-29a, ssc-mir-30e, ssc-mir-499, ssc-mir-143, ssc-mir-10b, ssc-mir-486-1, ssc-mir-152, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-99a, ssc-mir-148b, ssc-mir-664, ssc-mir-192, ssc-mir-342, ssc-mir-125b-1, oar-mir-29a, oar-mir-125b, oar-mir-181a-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-296, ssc-mir-155, ssc-mir-146a, bta-mir-148c, ssc-mir-126, ssc-mir-378-2, ssc-mir-451, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-451b, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-664b, hsa-mir-378j, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-31, ssc-mir-671, bta-mir-378b, bta-mir-378c, hsa-mir-486-2, oar-let-7a, oar-let-7f, oar-mir-103, oar-mir-10b, oar-mir-143, oar-mir-148a, oar-mir-152, oar-mir-16b, oar-mir-181a-2, oar-mir-200a, oar-mir-200b, oar-mir-200c, oar-mir-23a, oar-mir-26a, oar-mir-29b-1, oar-mir-30a, oar-mir-99a, bta-mir-664b, chi-let-7a, chi-let-7f, chi-mir-103, chi-mir-10b, chi-mir-125b, chi-mir-126, chi-mir-141, chi-mir-143, chi-mir-146a, chi-mir-148a, chi-mir-148b, chi-mir-155, chi-mir-15a, chi-mir-15b, chi-mir-16a, chi-mir-16b, chi-mir-184, chi-mir-192, chi-mir-200a, chi-mir-200b, chi-mir-200c, chi-mir-215, chi-mir-21, chi-mir-223, chi-mir-224, chi-mir-2318, chi-mir-23a, chi-mir-24, chi-mir-26a, chi-mir-27b, chi-mir-296, chi-mir-29a, chi-mir-29b, chi-mir-29c, chi-mir-30a, chi-mir-30e, chi-mir-342, chi-mir-378, chi-mir-451, chi-mir-499, chi-mir-671, chi-mir-99a, chi-mir-99b, bta-mir-378d, ssc-mir-378b, oar-mir-29b-2, ssc-mir-141, ssc-mir-200b, ssc-mir-223, bta-mir-148d
Ye et al. (2012) examined miRNA expression in the duodenum of E. coli F18-sensitive and -resistant weaned piglets and identified 12 candidate miRNA (ssc-miR-143, ssc-let-7f, ssc-miR-30e, ssc-miR-148a, ssc-miR-148b, ssc-miR-181a, ssc-miR-192, ssc-miR-27b, ssc-miR-15b, ssc-miR-21, ssc-miR-215, and ssc-miR-152) disease markers. [score:5]
Additionally, a number of miRNAs including miR-148a, miR-26a, miR-21-5p, miR-27b, miR-143, bta-miR-30a-5p, let-7a-5p, let-7f, miR-10b, and miR-99a-5p are highly expressed in bovine mammary gland/mammary epithelial cells (Li et al., 2012a, 2014a; Jin et al., 2014a; Le Guillou et al., 2014) suggesting roles in the lactation process and mammary gland functions. [score:3]
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5
[+] score: 5
Validation of a selected few by qPCR identified 10 miRNAs - miR-133b-3p, miR-208b-3p, miR-21-5p, miR-125a-5p, miR-125b-5p, miR-126-3p, miR-210-3p, miR-29a-3p, miR-494-3p and miR-320a, that were significantly up-regulated in HF myocardium compared to normal controls. [score:3]
Similarly, MiR-29a is reported to be a key regulator of cardiac fibrosis and hypertrophy 38, 39, and miR-21 is enhanced in the fibroblasts of failing hearts [40]. [score:2]
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6
[+] score: 4
Other miRNAs from this paper: hsa-mir-21, hsa-mir-145, hsa-mir-155
Future studies in the sheep asthma mo del may investigate the changes in expression and subsequent therapeutic targeting of other Th [2] -associated mediators such as IL-17, IL-22, IL-25 and IL-33 [60– 63], or miRNAs thought to play a role in directing the pathways of IL-4 and/or IL-13 production in allergic airways, including miR-21, miR-145 and miR-155 [64– 67]. [score:4]
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7
[+] score: 4
A number of these miRs had identified targets in the insulin signaling pathway, for example, miR-126-5p, miR-106b-5p miR-126-5p, miR-21-5p, and miR-369-3p (Lie et al. 2014). [score:3]
These include miR-126-5p, miR-30a-5p, and miR-30d (Guay et al. 2011), as well as miR-27b, miR-21, miR-206 (Herrera et al. 2010), and let-7 family (Frost and Olson 2011), each of which is altered in the states of insulin resistance, glucose intolerance, and/or type-2 diabetes in adult life. [score:1]
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8
[+] score: 3
We also successfully validated the expression of 4 known miRNAs in the LV sample (oar-miR-21, oar-miR-493-5p, oar-miR-494-3p, oar-miR-133-3p) (see Table 3). [score:3]
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9
[+] score: 3
Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-127, mmu-mir-134, mmu-mir-136, mmu-mir-154, mmu-mir-181a-2, mmu-mir-143, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-21a, rno-mir-329, mmu-mir-329, mmu-mir-1a-2, mmu-mir-181a-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-375, mmu-mir-379, mmu-mir-181b-2, rno-mir-21, rno-mir-127, rno-mir-134, rno-mir-136, rno-mir-143, rno-mir-154, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-196a, rno-mir-181a-1, mmu-mir-196b, rno-mir-196b-1, mmu-mir-412, mmu-mir-370, oar-mir-431, oar-mir-127, oar-mir-432, oar-mir-136, mmu-mir-431, mmu-mir-433, rno-mir-431, rno-mir-433, ssc-mir-181b-2, ssc-mir-181c, ssc-mir-136, ssc-mir-196a-2, ssc-mir-21, rno-mir-370, rno-mir-412, rno-mir-1, mmu-mir-485, mmu-mir-541, rno-mir-541, rno-mir-493, rno-mir-379, rno-mir-485, mmu-mir-668, bta-mir-21, bta-mir-181a-2, bta-mir-127, bta-mir-181b-2, bta-mir-181c, mmu-mir-181d, mmu-mir-493, rno-mir-181d, rno-mir-196c, rno-mir-375, mmu-mir-1b, bta-mir-1-2, bta-mir-1-1, bta-mir-134, bta-mir-136, bta-mir-143, bta-mir-154a, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-329a, bta-mir-329b, bta-mir-370, bta-mir-375, bta-mir-379, bta-mir-412, bta-mir-431, bta-mir-432, bta-mir-433, bta-mir-485, bta-mir-493, bta-mir-541, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-1, ssc-mir-181a-1, mmu-mir-432, rno-mir-668, ssc-mir-143, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-196b-1, ssc-mir-127, ssc-mir-432, oar-mir-181a-1, oar-mir-493, oar-mir-433, oar-mir-370, oar-mir-379, oar-mir-329b, oar-mir-329a, oar-mir-134, oar-mir-668, oar-mir-485, oar-mir-154a, oar-mir-154b, oar-mir-541, oar-mir-412, mmu-mir-21b, mmu-mir-21c, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-370, ssc-mir-493, bta-mir-154c, bta-mir-154b, oar-mir-143, oar-mir-181a-2, chi-mir-1, chi-mir-127, chi-mir-134, chi-mir-136, chi-mir-143, chi-mir-154a, chi-mir-154b, chi-mir-181b, chi-mir-181c, chi-mir-181d, chi-mir-196a, chi-mir-196b, chi-mir-21, chi-mir-329a, chi-mir-329b, chi-mir-379, chi-mir-412, chi-mir-432, chi-mir-433, chi-mir-485, chi-mir-493, rno-mir-196b-2, bta-mir-668, ssc-mir-375
Using gene sequence comparisons and expression analysis, Lee and Ambros [23] reported that about 12% of miRNA in C. elegans, Drosophila and some plants are conserved and of these, miR-21, miR-234, miR-260 and miR-287 are highly conserved [23]. [score:3]
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10
[+] score: 1
In the DU, CE, and CO libraries, miR-143 had the largest number of reads, while the miR-21, miR-148a, miR-26, and let-7 families were the next most abundant. [score:1]
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