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miRBase |
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![]() 5 publications mentioning ssc-mir-574Open access articles that are associated with the species Sus scrofa and mention the gene name mir-574. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: ssc-mir-122, ssc-mir-148a, ssc-mir-15b, ssc-mir-184, ssc-mir-216-1, ssc-mir-24-1, ssc-mir-140, ssc-mir-27a, ssc-mir-21, ssc-mir-30c-2, ssc-mir-210, ssc-mir-221, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-30a, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-27b, ssc-mir-24-2, ssc-mir-193a, ssc-mir-192, ssc-mir-100, ssc-mir-222, ssc-mir-335, ssc-mir-129a, ssc-mir-133a-2, ssc-mir-4332, ssc-mir-652, ssc-mir-146a, ssc-mir-30c-1, ssc-mir-451, ssc-mir-129b, ssc-mir-1343, ssc-mir-216-2, ssc-mir-582
When testing miRNAs that were down-regulated, no miRNAs had a significant number of target genes that were up-regulated, but 4 miRNAs (miR-222, miR-27a, miR-574-5p, and miR-485-3p) had a significant number of target genes that were also down-regulated when using both DEG lists of <5% and <10%.
[score:14]
Eight DEMs, 4 down-regulated (miR-146a, miR-222, miR-574 and miR-652) and 4 up-regulated (miR-100, miR-133a, miR-216 and miR-582-5p), were randomly chosen for validation using RT-qPCR.
[score:7]
Among the 8 DEMs detected by deep sequencing, 4 were proved significant (p<0.05) by qPCR, one had a tendency to be significant (p = 0.06), yet 3 (miR-146a, miR-574 and miR-100) were not confirmed significant (Table 2).
[score:1]
Among the 40 DEMs obtained by deep sequencing, 8 (miR-100, miR-140, miR-184, miR-193a-5p, miR-222, miR-4332-3p, miR-451, and miR-574) were detectable in microarray.
[score:1]
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From the 119 miRNAs highly expressed (>80 reads) in MLN, 8 miRNAs were DE, five up-regulated (mir-126-3p, mir-126-5p, let-7d-3p, mir-129a, mir-let-7b-3p) and three down-regulated (mir-193a-5p, mir-574-5p and mir-34a) (Table 3).
[score:9]
Differential expression of miRNAs in MLN is high due to the PCV2 infection; from the most represented miRNAs in both tissues (CN > 80): mir-126-3p, mir-126-5p, mir-let-7d-3p, miR-129-2-3p and mir-let-7b-3p were up-regulated in MLN of PCV2 infected animals, while miR-193a-5p, miR-574-5p and miR-34a-5p were down-regulated.
[score:9]
miR-574-5p was down-regulated in MLN and was predicted to be involved in the regulation of the T cell receptor signalling and MAPK signalling pathways.
[score:5]
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Other miRNAs from this paper: hsa-mir-24-2, hsa-mir-29b-1, mmu-mir-1a-1, mmu-mir-29b-1, mmu-mir-133a-1, mmu-mir-135a-1, mmu-mir-142a, mmu-mir-145a, mmu-mir-146a, mmu-mir-155, hsa-mir-30d, hsa-mir-139, hsa-mir-223, hsa-mir-1-2, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-135a-1, hsa-mir-142, hsa-mir-145, hsa-mir-146a, mmu-mir-30d, mmu-mir-24-2, mmu-mir-323, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-155, mmu-mir-139, mmu-mir-223, mmu-mir-7a-1, mmu-mir-7a-2, hsa-mir-323a, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, ssc-mir-145, ssc-mir-29b-1, ssc-mir-323, ssc-mir-139, hsa-mir-491, hsa-mir-574, hsa-mir-654, mmu-mir-491, mmu-mir-466h, mmu-mir-574, mmu-mir-654, mmu-mir-877, hsa-mir-877, mmu-mir-1b, mmu-mir-1187, ssc-mir-133a-1, ssc-mir-1, mmu-mir-2137, ssc-mir-133b, ssc-mir-30d, ssc-mir-24-2, ssc-mir-142, hsa-mir-323b, ssc-mir-155, ssc-mir-133a-2, ssc-mir-146a, ssc-mir-491, mmu-mir-145b, mmu-mir-142b, ssc-mir-223
We found that 86.11% (31 of 36) of the relative real-time RT-PCR results were consistent with those obtained in the microRNA microarray analysis in terms of direction of regulation at one or more time points except the results of miR-574-3p in BJ501-infected lung on 2 dpi, miR-1 in PR8-infected lung on 2 dpi, miR-1 in BJ501-infected lung on 5 dpi, miR-133a in PR8-infected lung on 2 dpi and miR-133b in PR8-infected lung on 2 dpi (Figure 5).
[score:3]
Nine microRNAs (miR-1, miR-1187, miR-133a, miR-133b, miR-155, miR-2137, miR-223, miR-30d and miR-574-3p) were selected for validation.
[score:1]
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Other miRNAs from this paper: hsa-mir-18a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-30d, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-205, hsa-mir-30b, hsa-mir-128-1, hsa-mir-146a, hsa-mir-193a, hsa-mir-128-2, hsa-mir-365a, hsa-mir-365b, hsa-mir-424, hsa-mir-18b, hsa-mir-450a-1, ssc-mir-24-1, ssc-mir-7-2, ssc-mir-205, ssc-mir-128-1, ssc-mir-18a, hsa-mir-146b, hsa-mir-193b, hsa-mir-450a-2, hsa-mir-532, hsa-mir-92b, hsa-mir-574, hsa-mir-542, hsa-mir-450b, hsa-mir-744, ssc-mir-30b, ssc-mir-450a, ssc-mir-146b, ssc-mir-30a, ssc-mir-30d, ssc-mir-128-2, ssc-mir-24-2, ssc-mir-193a, ssc-mir-365-2, ssc-mir-365-1, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-424, ssc-mir-542, ssc-mir-450b, ssc-mir-532, ssc-mir-744, ssc-mir-146a, hsa-mir-1343, ssc-mir-18b, ssc-mir-1343, ssc-mir-7-1
In addition, expression patterns of miR-574-3p, miR-574-5p, miR-744*, miR-30a, miR-30d, miR-205 and miR-532-3p are also inconsistent with our results 29.
[score:3]
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Other miRNAs from this paper: ssc-mir-148a, ssc-mir-181b-2, ssc-mir-24-1, ssc-mir-181c, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-30c-2, ssc-mir-30b, ssc-mir-221, ssc-mir-133a-1, ssc-mir-1, ssc-mir-181a-1, ssc-mir-30a, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-378-1, ssc-mir-133b, ssc-mir-30d, ssc-mir-30e, ssc-mir-499, ssc-mir-143, ssc-mir-10b, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-27b, ssc-mir-24-2, ssc-mir-208b, ssc-mir-127, ssc-mir-222, ssc-mir-155, ssc-mir-133a-2, ssc-mir-30c-1, ssc-mir-378-2, ssc-let-7a-2, ssc-let-7d, ssc-let-7f-2, ssc-mir-3613, ssc-mir-2366-1, ssc-mir-2366-2, ssc-mir-2411, ssc-mir-2483, ssc-mir-378b
It was interesting that ssc-miR-new_06, ssc-miR-new_18, ssc-miR-new_27 and ssc-miR-new_53 matched the same mature miRNA, hsa-miR-574-5p, but their precursors had no sequence homologous to each other or to precursors of has-miR-574-5p.
[score:1]
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