sort by

4 publications mentioning ssc-mir-361

Open access articles that are associated with the species Sus scrofa and mention the gene name mir-361. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 155
Similarly, inhibition of ssc-miR-361-3p was achieved by transfecting cells with 100 nM of 2′-O methylated single-stranded ssc-miR-361-3p antisense oligonucleotides (inhibitors, GenePharma, China), ssc-miR-361-3p inhibitors sequence is 5′-GCAAAUCAGAAUCACACUGGGGG-3′ and inhibitors negative control (I-NC) sequence is 5′-CAGUACUUUUGUGUAGUACAA-3′. [score:9]
0057156.g005 Figure 5 Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
Round boxes (let-7a,let-7c,miR-22-5p,miR-19b,miR-181d-5p,miR-338,miR-423-3p,miR-133b, miR-206,miR-1307, miR-130b,miR-425-3p, and miR-532-5p) represent up-regulated miRNAs, gray octangle boxes (miR-30e-3p, miR-320, miR-324, miR-361-3p, miR-152, miR-708-5p, and miR-22-3p) represent down-regulated miRNAs, black hexagon boxes(FSHβ, CREB, c-Jun, c-Fos) indicate target genes. [score:9]
0057156.g002 Figure 2 ssc-let-7c, ssc-miR-361-5p, ssc-423-3p, ssc-miR-425-3p and ssc-miR-451 were up-regulated, while ssc-miR-30e-3p, ssc-miR-320, ssc-miR-324 and ssc-miR-361-3p were down-regulated after GnRH challenge. [score:7]
More interestingly, ssc-miR-361-5p and ssc-miR-22-5p were up-regulated, while ssc-miR-361-3p and ssc-miR-22-3p were down-regulated. [score:7]
ssc-let-7c, ssc-miR-361-5p, ssc-423-3p, ssc-miR-425-3p and ssc-miR-451 were significantly up-regulated in the GnRH treatment group, while ssc-miR-30e-3p, ssc-miR-320, ssc-miR-324 and ssc-miR-361-3p were significantly down-regulated (Figure 2 ). [score:7]
The panels with different letters were considered statistically significant (p<0.05), N = 6. ssc-let-7c, ssc-miR-361-5p, ssc-423-3p, ssc-miR-425-3p and ssc-miR-451 were up-regulated, while ssc-miR-30e-3p, ssc-miR-320, ssc-miR-324 and ssc-miR-361-3p were down-regulated after GnRH challenge. [score:6]
For example, miR-133b is able to target FSHβ, c-Jun and c-Fos, while miR-361-3p may target c-Jun and FSHβ. [score:5]
In the following trials of pig pituitary primary cells, increase of miR-361-3p expression inhibited FSH secretion. [score:5]
Up-regulation of ssc-mir-361-3p was achieved by transfecting cells with 100 nM of synthetic RNA duplex (mimics, GenePharma, China) and negative control by Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. [score:4]
FSH concentration of cell supernatant were measured at 24 h after transfection, the result showed that up-regulation of ssc-miR-361-3p led to a significant decrease of FSH secretion by 14.1% (p<0.05) (Figure 7A ), while ssc-miR-361-3p inhibitors significantly increased FSH secretion by 15.1% (p<0.05) (Figure 7C ). [score:4]
C. miR-361-3p inhibitors increased FSH secretion by 15.07% compared with inhibitor negative control (I-NC) (163.31±7.04 vs. [score:4]
Our prediction also showed that miR-361-3p may have other targets involved in FSH secretion regulation. [score:4]
Interestingly, dramatic change of ssc-miR-361-3p expression caused only ∼15% FSH secretion increase or decrease, suggesting that ssc-miR-361-3p probably is a fine-tuned regulator in FSH secretion. [score:4]
The results strongly suggested that miR-361-3p participated in FSH secretion regulation, most possibly by targeting the 3′-UTR of FSH mRNA. [score:4]
In addition, the abundance of ssc-miR-361-3p was increased by mimics (Figure 7B ) and decreased by inhibitors (Figure 7D ). [score:3]
d AAAGUGC ssc-miR-361-3p 0.76 miR-361-3p CCCCCAG To validate the differentially expressed miRNAs revealed via microarray, 10 miRNAs were selected randomly (ssc-let-7c, ssc-miR-30e-3p, ssc-miR-320, ssc-miR-324, ssc-miR-361-3p, ssc-miR-361-5p, ssc-423-3p, ssc-miR-425-3p, ssc-miR-451 and ssc-miR-708-5p) for further analysis by using a special tail-added Real-time PCR method, taking U6 as an endogenous control. [score:3]
Both miRNA array and qPCR results showed that miR-361-3p expression decreased during FSH secretion after GnRH treatment. [score:3]
Most importantly, we identified a highly conserved X-linked miRNA, miR-361-3p, potentially targeting FSHβ. [score:3]
To further ascertain whether the alteration of ssc-miR-361-3p expression influences pituitary cell FSH secretion, ssc-miR-361-3p mimics and negative control were separately transfected to primary pig anterior pituitary cell. [score:3]
Microarray revealed a total of 31 differentially expressed miRNAs, most of which had been detected in pig pituitary anterior lobe with high abundance in our previous study [20], such as let-7a, let-7c, miR-361-5p, miR-320, miR-21, miR-22, miR-152 and miR-15a. [score:3]
Further experiments are needed to confirm the interaction between miR-361-3p and its potential targets. [score:3]
It was demonstrated that miR-361-3p was differentially expressed in several tumors or cancer [47]– [49]. [score:3]
D. Transfection of miR-361-3p inhibitors exhibited 80.87% decline of miR-361-3p level relative to I-NC (p<0.05). [score:3]
At 24 h following transfection, the supernatant of cells were collected for FSH hormone determination and cells were collected for RNA extraction to detect the expression of ssc-miR-361-3p by using stem-loop RT-qPCR [20]. [score:3]
Interestingly, 1 miRNA (miR-361-3p) may target the GnRH signaling “switch” GNRHR. [score:3]
Bioinformatic prediction showed that miR-361-3p potentially targets the FSHβ mRNA 3′-UTR. [score:3]
Notably, miR-361-3p and its target site in FSHβ mRNA were highly conserved across mammalian species. [score:3]
Among the 13 possible miRNAs targeting FSHβ mRNA, interestingly, miR-361-3p had a highly conserved binding site in the FSHβ mRNA 3′-UTR (Figure 6 ) in mammals with perfect match in seed sequence across pig, sheep, cattle, mouse and human. [score:3]
ssc-miR-361-3p involved in FSH secretion regulation. [score:2]
Furthermore, we showed for the first time that miR-361-3p negatively regulated FSH secretion in pig pituitary cell mo del. [score:2]
ssc-miR-361-3p involved in FSH secretion regulation of porcine anterior pituitary cell. [score:2]
These results indicated that ssc-miR-361-3p was involved in FSH secretion regulation in pig anterior pituitary cell mo del. [score:2]
miR-361-3p and its potential role in FSH secretion regulation. [score:2]
In addition, 4 miRNAs were mapped to chromosome X, including ssc-miR-19b, ssc-mir-105-2 as well as ssc-miR-361-5p and ssc-miR-361-3p, which were produced from the same precursor (ssc-miR-361). [score:1]
A. ssc-miR-361-3p mimics (mimics) and duplex negative control (NC) were separately transfected to primary pig pituitary cell, cell supernatant were collected for FSH determination at 24 h after transfection, ssc-miR-361-3p mimics led to significant decrease of FSH secretion by 14.10% (140.00±11.45 vs. [score:1]
B. Transfection of miR-361-3p mimics increased miR-361-3p level in pituitary cell by 3.22-folds (p<0.05). [score:1]
Most importantly, the miR-361-3p precursor was located on the X chromosome in all species, which suggests miR-361-3p is X-linked and plays a potential role in controlling reproductive event based on gender. [score:1]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
High conservation of miR-361-3p and its potential binding site in FSHβ mRNA 3′-UTR across mammalians. [score:1]
Up to date, little is known about miR-361-3p function. [score:1]
0057156.g006 Figure 6 A. is alignment for mature miR-361-3p (named miR-361* in rat). [score:1]
Interestingly, chromosomal location analysis detected two couples of miRNAs, miR-22-5p/miR-22-3p and miR-361-5p/miR-361-3p, which come from the same precursor. [score:1]
h CAAAUGC ssc-miR-19b 1.50 miR-19 GUGCAAA ssc-miR-183 1.47 miR-183 AUGGCAC ssc-miR-423-3p 1.46 miR-423/423-3p GCUCGGU ssc-miR-206 1.46 miR-1/206 GGAAUGU ssc-miR-15a 1.42 miR-15/16/195/424/497 AGCAGCA ssc-miR-22-5p 1.39 miR-22-5p/3568 GUUCUUC ssc-miR-133a-3p 1.39 miR-133 UUGGUCC ssc-miR-340 1.37 miR-340/340-5p UAUAAAG ssc-let-7a 1.37 let-7/98 GAGGUAG ssc-miR-338 1.36 miR-338/338-3p CCAGCAU ssc-miR-361-5p 1.35 miR-361/361-5p UAUCAGA 10.1371/journal. [score:1]
Interestingly, ssc-miR-361-5p and ssc-miR-361-3p shared the same precursor, so did ssc-miR-22-5p and ssc-miR-22-3p. [score:1]
Notably, miRBase data showed all miR-361-3p precursors of different organisms located on X chromosome, which suggested that this miRNA may play an important role in reproductive activity based on gender. [score:1]
A. is alignment for mature miR-361-3p (named miR-361* in rat). [score:1]
0057156.g007 Figure 7 A. ssc-miR-361-3p mimics (mimics) and duplex negative control (NC) were separately transfected to primary pig pituitary cell, cell supernatant were collected for FSH determination at 24 h after transfection, ssc-miR-361-3p mimics led to significant decrease of FSH secretion by 14.10% (140.00±11.45 vs. [score:1]
ssc-mir-361-3p mimics sequences were as follows, 5′-CCCCCAGGUGUGAUUCUGAUUUGC-3′(sense), 5′-AAAUCAGAAUCACACCUGGGGGUU-3′ (antisense) and negative control sequence are 5′-UUCUCCGAACGUGUCACGUTT-3′(sense) and 5′-ACGUGACACGUUCGGGAATT-3′(antisense). [score:1]
[1 to 20 of 49 sentences]
2
[+] score: 4
P38 MAPKs (MAPK11, MAPK13, and MAPK14) were found to be regulated by miR-769-5p, miR-146b-5p, let-7g, miR-30b, miR-31, miR-361-3p, and miR-362-3p (Figure 7), which were all down expressed in H1N1 critically ill patients. [score:4]
[1 to 20 of 1 sentences]
3
[+] score: 3
Interestingly, some miRNAs were found to target more than one gene of SIV-H1N1/2009, for example, the binding sites for ssc-miR-361-3p located in both viral PB2 and HA, and the binding sites for ssc-miR-136 located in both viral NA and NP. [score:3]
[1 to 20 of 1 sentences]
4
[+] score: 1
While the largest increase in abundance was observed with let-7c in both ORF1 and ORF3 libraries, and with miR-411-5p in the ORF2 library, miR-196a, miR-361-3p, and miR-1224-5p showed the largest decrease in abundance in the ORF1, ORF2 and ORF3 libraries, respectively. [score:1]
[1 to 20 of 1 sentences]