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11 publications mentioning bmo-mir-2a-2

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-2a-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 64
Sli-miR-2a expressed at low level in egg, prepupa and adult stages, instead expressed most strongly in pupa stage, which accord with that in mo del insects, D. melanogaster [30] and B. mori [24]. [score:5]
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraThe expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:5]
There were significant differences among various developmental stages, the relative expression levels of sli-miR-2a were 2.89-, 15.65-, 24.55-, 4.88-, 2.23-, 38.68- and 1.81-fold higher in the first instar, third instar, fourth instar, six instar, prepupa, pupa and adult than in egg, respectively (Figure 5b). [score:4]
Development-specific expression patterns for sli-miR-2a were also detected by qPCR. [score:4]
Three known miRNAs, miR-14, miR-2a and bantam, play important roles in the developmental stages in D. melanogaster [23, 24], such as regulating steroid hormone signaling [25] and apoptosis [12, 26, 27]. [score:3]
The expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:3]
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:3]
It has been clearly stated that cell autonomous anti-apoptotic activity mediated by miR-14 and miR-2a [12, 27], miR-14 also plays critical role in molting process [25], indicating miRNAs are involved in strict developmental regulation in insects. [score:3]
Moreover, expression patterns analysis indicated that the highly conserved miRNAs, both miR-14 and miR-2a, could be detected successfully by real-time quantitative PCR, which confirms that stem-loop RT-PCR can be used not only for quantification of miRNAs, but also for identifying highly conserved miRNAs in non-mo del insects. [score:3]
Therefore, Primers Pre-2aF and Pre-2aR were designed according to the conserved miRNAs for amplifying pre-miR-2a-2, and rising nealing temperature to 65 °C to inhibit non-specific amplification. [score:2]
However, the expression patterns of sli-miR-14 and sli-miR-2a, two highly conserved miRNAs in insect, could be obtained in this assay. [score:2]
The homologues of miR-14, miR-2a and bantam were cloned from Spodoptera litura, a prevalent agriculture pest in China, by stem-loop RT-PCR, and their expression patterns in different developmental stages were also investigated to confirm the results of sequence analysis. [score:2]
The mature sli-miR-14 and sli-miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraTo identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
MiR-14 and miR-2a are more conserved members of miRNAs family than bantam in mo del insects including D. melanogaster and B. mori (Table S1). [score:1]
To identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
The putative homologue of the three miRNAs in S. litura, namely sli-miR-14, sli-miR-2a and sli-bantam were cloned by a stem-loop RT-PCR technique. [score:1]
A 552bp fragment containing putative pre-sli-miR-2a-2 was amplified from genomic DNA of adult of S. litura. [score:1]
Genomic DNA Isolation and the Amplification of pre-miR-14 and pre-miR-2a from S. lituraTotal genomic DNA was isolated from the adult of S. litura according to the instruction of E. Z. N. A. Insect DNA Kit (OMEGA, USA). [score:1]
A cluster of miR-2a-1, miR-2a-2, miR-2b, miR-13a and miR-13b is localized on chromosome 1 of B. mori, indicating there are two precursors of miR-2a, and miR-2a-2 is in the interior of this cluster. [score:1]
When Group 1 was used for amplification of miR-14, miR-2a and bantam, PCR products were 86 bp, 89 bp, 89 bp, respectively (Figure 1a); when Group 2 was used, PCR products were 76 bp, 79 bp and 79 bp in length, respectively (Figure 1b). [score:1]
Results showed that putative pre-miR-14 and pre-miR-2a of S. litura can form stable stem-loop structures (initial Δ G = −41.80 and Δ G = −41.20 respectively) and are highly homologous to those of B. mori and D. melanogaster, indicating they are the precursors of sli-miR-14 and sli-miR-2a (Figure 4). [score:1]
The putative pre-sli-miR-2a showed 81.1% and 70.4% similarities with pre-bmo-miR-2a and pre-dme-miR-2a respectively (Figure 3b). [score:1]
In order to confirm whether the PCR products are endogenous miRNAs, putative precursors of miR-14 and miR-2a as representatives were also cloned from S. litura, results showed that both their sequences and secondary structures were highly conserved with mo del insects. [score:1]
Moreover, both mature sequences of miR-14 and miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
Cloning and Analysis of pre-miR-14 and pre-miR-2a from S. lituraTo verify the present results, homology of pre-miR-14 and pre-miR-2a were cloned from S. litura. [score:1]
Genomic DNA Isolation and the Amplification of pre-miR-14 and pre-miR-2a from S. litura. [score:1]
To verify the present results, homology of pre-miR-14 and pre-miR-2a were cloned from S. litura. [score:1]
The efficiency of miR-14, miR-2a and 5S rRNA was close to the ideal value of 2 (Table 1), therefore relative quantification (RQ) of miRNA expression was calculated with 2 [−Δ ΔCt] method [28, 29]. [score:1]
By searching bmo-miR-2a in Silkworm Genome Database, it is noticed that there are two precursors of pre-bmo-miR-2a which localized on chromosome 1 and organized as a cluster with pre-miR-2b, pre-miR-13a and pre-miR-13b (Figure 5). [score:1]
Although the identification of miRNAs in non-mo del insects with stem-loop RT-PCR is limited by 5′ region of putative miRNA, large amounts of miRNAs that are highly conserved, such as miR-14 and miR-2a, can be simply cloned by this method. [score:1]
Cloning and Analysis of pre-miR-14 and pre-miR-2a from S. litura. [score:1]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:1]
Based on the conserved character of miRNA, Pre-2aF and Pre-2aR for cloning pre-miR-2a-2 were designed according to the mature sequences of miR-2b and miR-2a-1 which located at both ends of the cluster. [score:1]
Pre-miR-14 and pre-miR-2a as representatives were also cloned from S. litura; both their sequences and secondary structures shared a high degree of homology with those in mo del insects, and the mature sequences of miR-14 and miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
Three homologues of known miRNAs, miR-14, miR-2a and bantam in mo del insects, were cloned from S. litura by stem-loop RT-PCR, and named sli-miR-14, sli- miR-2a and sli-bantam. [score:1]
PCR of sli-miR-14, sli-miR-2a and sli-bantam. [score:1]
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2
[+] score: 21
During metamorphosis, bmo-miR-2a was highly expressed in the four tissues, but no expression signals were detected for its antisense transcript; both miR-276-5p and its antisense transcript were absent or expressed at low levels in the body wall, and fat body, but strongly accumulated in the silk gland and midgut. [score:7]
miR-2a was ubiquitously expressed in all tissues, with the strongest signals found in the head and body wall, followed by the gonads and malpighian tubules, and then the silk glands, fat body and midgut, but the antisense strand was not found in these tissues. [score:3]
Although miR-2a and miR-13a, miR-10a and miR-10b are closely clustered in the silkworm genome [30], their expression patterns were distinct. [score:3]
Approximately 20 miRNAs were significantly expressed in the body wall of males (e. g., miR-2a, miR-34b and miR-10b-5p/3p). [score:3]
Additionally, Northern-blot results confirmed sex differences in body wall expression of 10 miRNAs (e. g., bantam, miR-1, miR-13a and miR-2a) (Table 2, Figure 1D). [score:3]
Similar results were obtained by Northern blot analysis for bantam, miR-2a, miR-228 and miR-79 (Figure 1D). [score:1]
Based on microarray analysis, 10 female-biased miRNAs were detected in the malpighian tubules (miR-286, miR-228, miR-274, miR-1, miR-252, let-7a, miR-8, bantam, miR-200b, and miR-2a), whereas miR-276-5p and miR-305 showed male-prone accumulation in malpighian tubules. [score:1]
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3
[+] score: 19
mir-2 and mir-13 repress translation of the target genes, grim, skl and rpr, suggesting that they may be involved in regulating apoptosis [48]. [score:6]
bmo-miR-2b, which is found in the gene cluster bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b, encodes a newly identified member of the mir-2 family. [score:1]
mir-2 and mir-7 may be related to the Notch signaling pathway in B. mori; this hypothesis is consistent with previous predictions [38, 53]. [score:1]
4220] of these six genes can bind perfectly to mir-2 (bmo-miR-2a, bmo-miR-2b, bmo-miR-13a* and bmo-miR-13b) and bmo-miR-7, respectively. [score:1]
mir-2 controls HLHm delta, and mir-7 controls HLHm3, HLHm5, HLHmgamma, M4 and TOM, in D. melanogaster; these six genes are involved in the Notch signaling pathway. [score:1]
These newly identified miRNAs are bmo-miR-2a*, bmo-miR-8*, bmo-miR-13a*, bmo-miR-46*, bmo-miR-263*, bmo-miR-279*, and bmo-miR-305*. [score:1]
a. bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b; b. bmo-miR-275/bmo-miR-305/bmo-miR-305*. [score:1]
Examining the positions of the identified miRNAs in the B. mori genome, we identified two miRNA clusters (Figure 4): bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b; and bmo-miR-275/bmo-miR-305/bmo-miR-305*. [score:1]
Searching miRBase, we found that the bmo-miR-2a sequence was identical to the dme-miR-2a, dps-miR-2a, ame-miR-2, and aga-miR-2 sequences, but we failed to identify any other miRNA that had the same sequence as bmo-miR-2b (Figure 5); therefore, bmo-miR-2b is a newly identified member of the mir-2 miRNA family. [score:1]
Genes in the cluster containing bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b are members of the mir-2 miRNA family. [score:1]
This cluster contains bmo-miR-2a, which can be derived from two different precursor miRNAs transcribed from two paralogous miRNA genes, bmo-miR-2a-1 and bmo-miR-2a-2. The opposite strand to bmo-miR-2a-1 also encodes a miRNA. [score:1]
Figure 5Homologous analysis of mir-2 family. [score:1]
We identified two miRNA gene clusters in the B. mori genome, and bmo-miR-2b was identified as a new member of the mir-2 family. [score:1]
Searching the D. melanogaster and A. gambiae miRNAs, we found that corresponding mir-2 and mir-13 family members also assembled into clusters in these two species. [score:1]
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4
[+] score: 10
Preferential expression of miR-2 and miR-13 families in spinning larvae and moth suggests that these miRNAs may promote apoptosis in these two stages. [score:3]
miR-2 and miR-13 families possess identical "seed" sequences, and both show almost similar expression profiles in the silkworm. [score:3]
The miR-2 family is known to target proapoptotic genes (reaper, grim and sickle) in Drosophila [69]. [score:3]
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
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5
[+] score: 8
Coincidently we detected bmo-miR-13 in two stages: late fifth-instar larva (LFLS) and pupa stages (PS), and its potential targets also include notch-like gene and inhibitor of apoptosis gene (Iap) (Table 4) suggesting that bmo-miR-2/13 have similar functions in silkworms and fruitflies. [score:5]
The miR-2 family has been implicated in the control of apoptosis [23], [64]. [score:1]
We also discovered six pairs that are organized as clusters; bmo-miR-1/bmo-miR-133, bmo-let-7/bmo-miR-100, bmo-miR-12/bmo-miR-283, and bmo-miR-275/bmo-miR-305 are separated by less than 20 kb apart and in the same orientation; bmo-miR-9b overlaps with bmo-miR-79 on the opposite strand; and bmo-miR-2 is adjacent to bmo-miR-13 but on the reverse strand in a tail-to-tail orientation about some twenty basepairs away. [score:1]
Another pair of interesting clustered miRNAs in the silkworm genome contains bmo-miR-2 and bmo-miR-13, which are classified into the miR-2 family based on sequence similarity. [score:1]
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6
[+] score: 5
Similarly, miR-2 and miR-7 control signal transduction pathways (notch signalling) in both Drosophila sp and B. mori by targeting HLHmδ, HLHm3, HLHm5, HLHmγ, M4 and TOM [33, 34]. [score:3]
In this specific instance, miR-2a and miR-34 were identified from Helicoverpa armigera and S. litura based on in silico comparative analysis using data set of B. mori. [score:1]
The cluster (sfr-mir-2a, sfr-mir-2b, sfr-mir-2c, sfr-mir-13a, sfr-mir-13b) is distributed over the region, 8161–8774 of scaffold 1973 with a size of 20411bp (Fig. 3B, i), while the other (sfr-mir-10494, sfr-mir-10463, sfr-mir-10471) span over 10636bp region of scaffold 6745. [score:1]
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7
[+] score: 5
Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-9a, dme-mir-10, dme-mir-12, dme-mir-13a, dme-mir-13b-1, dme-mir-13b-2, dme-mir-276a, dme-mir-133, dme-mir-276b, dme-mir-210, dme-mir-31b, dme-mir-9c, dme-mir-306, dme-mir-9b, dme-mir-31a, dme-mir-309, dme-mir-316, dme-mir-317, dme-mir-2c, ame-mir-12, ame-mir-133, ame-mir-210, ame-mir-276, ame-mir-2-1, ame-mir-2-2, ame-mir-317, ame-mir-9a, ame-mir-9b, bmo-mir-9a, bmo-mir-10, bmo-mir-276, bmo-mir-31, bmo-mir-71, ame-mir-10, ame-mir-137, ame-mir-13a, ame-mir-2-3, ame-mir-29b, ame-mir-31a, ame-mir-375, ame-mir-71, ame-mir-932, dme-mir-193, dme-mir-375, dme-mir-932, dme-mir-970, dme-mir-971, dme-mir-989, dme-mir-137, dme-mir-1006, dme-mir-1007, bmo-mir-2a-1, bmo-mir-2b, bmo-mir-13a, bmo-mir-13b, bmo-mir-133, bmo-mir-210, bmo-mir-317, tca-mir-2-3, tca-mir-2-1, tca-mir-2-2, tca-mir-10, tca-mir-12, tca-mir-13a, tca-mir-13b, tca-mir-31, tca-mir-71, tca-mir-133, tca-mir-137, tca-mir-210, tca-mir-276, tca-mir-317, tca-mir-932, tca-mir-9b, bmo-mir-12, bmo-mir-137, bmo-mir-932, bmo-mir-9b, tca-mir-9a, tca-mir-970, ame-mir-13b, ame-mir-1006, ame-mir-316, bmo-mir-970, lmi-mir-276, lmi-mir-210, lmi-mir-10, lmi-mir-9a, bmo-mir-9c, bmo-mir-306a, bmo-mir-989a, bmo-mir-316, bmo-mir-1175, bmo-mir-9d, bmo-mir-750, bmo-mir-375, bmo-mir-306b, api-mir-137, api-mir-10, api-mir-276, api-mir-13a, api-mir-210, api-mir-29, api-mir-2a, api-mir-2b, api-mir-2c, api-mir-316, api-mir-317, api-mir-71, api-mir-971, api-mir-9a, api-mir-9b, api-mir-306, api-mir-3049, bmo-mir-989b, ame-mir-1175, ame-mir-193, ame-mir-989, ame-mir-3049, ame-mir-971, ame-mir-3770, ame-mir-9c, ame-mir-306, ame-mir-750, tca-mir-9c, tca-mir-316, tca-mir-9d, tca-mir-309a, tca-mir-3049, tca-mir-375, tca-mir-29, tca-mir-1175, tca-mir-750, tca-mir-989, tca-mir-309b, tca-mir-193, tca-mir-6012, tca-mir-9e, ame-mir-6037, ame-mir-6012, ame-mir-2b, tca-mir-309c, tca-mir-971b
The structure of the miR-2 cluster in the seven species of insects studied. [score:1]
There is no correlation between the number of miRNA genes in a given family and the time of emergence of the family (MIR-2 family, which is the one that have more genes, appeared with the protostomes, whereas there are many monogenic miRNA families that emerged with the bilaterians) (Fig. 4). [score:1]
Transposable elements could be one of the sources of the miRNA cluster expansions as we observed on B. germanica Mir-71/Mir-2 cluster. [score:1]
Then, 5 families (8%) have between 2 and 3 genes, 4 families (6%) have between 3 and 5, and only one family (MIR-2) that have between 5 and 16 genes (Supplementary Table S8). [score:1]
In the cluster of Blattella germanica it is remarkable the distance between the second and the third copy of miR-2, where it is inserted a TIGD4 sequence. [score:1]
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8
[+] score: 3
In the previous studies, researcher suggesting the highly expressed Bmo-miR-2 in the egg stage possibly related to the generation of embryos [8]. [score:3]
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9
[+] score: 2
After a further 12 h (24 h Em), 12 miRNAs were detected soon after egg release from diapause, including the 7 "early" miRNAs and others observed for the first time (miR-252, miR-8, miR-2a, miR-79, and miR-10b-3p). [score:1]
A number of miRNAs were also confirmed in females by (let-7a, let-7b, miR-8, and miR-2a) (Figure 5B). [score:1]
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10
[+] score: 1
Seven families of conserved miRNAs, including four clusters described above (2, 4, 7, and 8), have paralogs in the silkworm genome (mir-2, mir-993, mir-9, mir-92, mir-263, mir-279, and mir-989) (Figure 4). [score:1]
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11
[+] score: 1
We observed a similar mechanism in silkworm TE-miRNAs, as we previously reported in non TE-derived miRNAs [37], that a miRNA precursor is able to yield different kinds of mature miRNA sequences (such as TE-miRNA-1 and TE-miRNA-2), and likewise different precursors produce the same miRNAs. [score:1]
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