4 publications mentioning vvi-MIR393b

Open access articles that are associated with the species Vitis vinifera and mention the gene name MIR393b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Auxin -regulating miRNAs are clearly modulated in our data: miR390, targeting ARF via ta-siRNA production, and miR393, targeting auxin receptors are both up-regulated (Fig.   8B) at late inflorescence development (Flower_FB and Flower_F), with open flowers, thus suggesting a role for this hormone in the first stages of inflorescence development.

Some miRNAs are specific to a few groups of samples, such as vvi-miR395-3p, which is strongly regulated during flower and berry development, or miRC477-3p mainly expressed in berries and rachis, and miR393 a,b-5p expressed in developing rachis and flowers.

miR390 regulates the Auxin Response Factor (ARF) through Trans-Acting siRNA locus 3 (TAS3), as experimentally validated in [28], while miR393 targets the auxin receptor TIR and an F-box protein [28] highlighting the importance of auxin regulation during flower development.

Other two interesting miRNAs are vvi-miR390 and vvi-miR393, both involved in auxin signalling regulation, are up-regulated during inflorescence and flower development (Figs.   8B and 9C).

A previous work in the hybrid Summer Black (V. vinifera x V. labrusca) showed a down-regulation of miR390 and miR393 in open flowers, compared to young inflorescences [27].

2

Overexpression of miR393 caused down-regulation of auxin receptor mRNAs, including transport inhibitor response 1 (TIR1), through degradation, which caused increased resistance to virulent Pseudomonas syringae pv.

Among them, miR160, miR167, miR390 and miR393 contribute to PTI by regulating the expression of genes encoding different auxin response factors (ARFs) and auxin receptors involved in auxin signalling, thereby promoting inhibition of pathogen growth [90].

miR393 expression, induced by bacterial elicitor flg22, was the first shown to be implicated in the repression of auxin receptor genes in Arabidopsis [23].

The bacterial PAMP peptide flg22 causes induced expression of the Arabidopsis miR393, which was the first miRNA identified to play a role in plant PTI.

3

Sunkar and Zhu (2004) reported that miR393, miR397b, and miR402 from Arabidopsis are upregulated by cold temperature, dehydration, high salinity, and abscisic acid (ABA); whereas miR389a is downregulated under the same stresses.

Several conserved miRNAs, including miR169, miR319, miR393, and miR408, were reported to respond to cold stress in other species; however, no visible expression change was found in our experiment.

4

For some families, thousands or even hundreds of thousands of short RNA sequences were recovered (miR156, miR164, miR166, miR167, miR172, miR393, miR396), while less than 1000 sequences corresponded to each of the remaining represented families.

In some cases, the most commonly observed sequences were identical to at least one of the predicted mature sequences (notably: miR156, miR160, miR164, miR167, miR169, miR172, miR394, miR399) while for other families, the predominant mature miRNA sequenced exhibited small variations (shifts or differences of length of one or two bases) with respect to the predicted mature sequences (e. g. miR166, miR393, miR395, miR396, miR408).