7 publications mentioning vvi-MIR319f

Open access articles that are associated with the species Vitis vinifera and mention the gene name MIR319f. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Under cold stress, miR319 expression decreased at time points of 8, 12 and 24 h. The expression levels of miR319 normalized by different reference genes were not exactly the same (Fig.   5b); although the normalization results of U6 snRNA and miR168 were similar, the expression normalized by miR160e and miR160e +  miR168 showed no significant differences at most time points.

As shown in Fig.   5, under salinity and cold stresses, the quantification results of target gene miR319 exhibited similar patterns when normalized by the selected reference genes or gene sets, demonstrating that the tested reference gene or gene set was mutually validated.

The columns represent the relative expression of miR319.

Therefore, miR319 (salinity and cold stress) and miR408 (drought stress) were chosen as the target genes to validate the stability of selected reference genes.

Thus, miR319 and miR408 were chosen as target genes to perform qRT-PCR to validate the normalization efficiency of selected reference genes.

When normalized by U6 snRNA, miR164a, miR160e and miR160e +  miR164a, the expression of miR319 under salinity stress increased through time until the peak was reached at 24 h, followed by a slight decrease at 48 h (Fig.   5a).

According to previous studies, miR319 responds to salinity and cold stresses, whereas miR408 is associated with drought stress in different plant species [37].

As reported by Thiebaut et al. [61], Zhou et al. [62] and Ma et al. [37], miR319 is responsive to salt and cold stress, whereas miR408 is responsive to drought stress.

2

In infected plants, miR166, miR319, miR396, miR3631, mi3633 and miR3639 were down-regulated in SWS, whereas miR168, miR482, miR535, miR2111, miR3624 and miR3634 were up-regulated (Fig. 2, Supplementary Fig. S4).

For example, in infected grapevines, we observed that the regulation of some miRNAs and their targets involved in leaf development was influenced by virus infection (miR156, miR164, miR319, miR394, miR396; Fig. 2, Supplementary Fig. S4).

Notably, most differences in miRNA accumulation between infected and GRSPaV-free plants disappeared in SWS conditions and significant differences in expression remained only for miR166, miR319, miR390, and miR3639 (Supplementary Fig. S4).

Indeed, in WW conditions, most conserved miRNAs that were validated by qRT-PCR (miR164, miR168, miR172, miR319, miR390, miR394, miR2111, miR3624, miR3629, miR3639) were expressed at higher levels in infected plants than in GRSPaV-free plants (Fig. 2, Supplementary Fig. S4).

3

The expression of miR319 in sugarcane is also upregulated during cold stress (Thiebaut et al., 2012).

Several conserved miRNAs, including miR169, miR319, miR393, and miR408, were reported to respond to cold stress in other species; however, no visible expression change was found in our experiment.

Overexpression of Osa-miR319 could lead to enhanced cold tolerance in rice (Yang et al., 2013).

Overexpression of microRNA319 impacts leaf morphogenesis and leads to enhanced cold tolerance in rice (Oryza sativa L. ).

Regulation of miR319 during cold stress in sugarcane.

4

SAP11 destabilises CINCINNATA (CIN)-TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTOR (TCP) transcription factors 1 and 2, known to be regulated by miR319 in Arabidopsis, resulting in the suppression of Jasmonate (JA) production that create favourable conditions for insect vector proliferation [97– 99].

The differential expression of conserved miRNA families (vvi-miR156, miR159, vvi-miR160, vvi-miR171, vvi-miR172, vvi-miR319), known to be involved in different aspects of plant development [18], make these potential candidates that play a role in the interactions leading to symptoms associated with GY.

Levels of vvi-miR159 and vvi-miR319 were also significantly higher in the AY phytoplasma-infected leaves, and in silico analysis predicted that they may target a GAMYB-like mRNA and a R2R3-MYB mRNA.

Consequently, altered expression of miR159 and miR319 may also contribute to deformation of grapevine leaves [82, 86].

5

For example, miR319c, miR319e and miR319f are all expressed in stem, while miR319c and miR319g are expressed in callus, no expression of miR319 was detected in leaf or root.

6

For example, miR319c, miR319e and miR319f are all expressed in stem, while miR319c and miR319 g are expressed in callus, no expression of miR319 was detected in leaf or root.

7

1-3p and four different members of miR319 family (above 59,000 or 70,000 TP5M), whose 3p sequence is predicted to target TCP4-like and a GAMYB-like transcription factors, are the most expressed in stamen and carpel.