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5 publications mentioning vvi-MIR168

Open access articles that are associated with the species Vitis vinifera and mention the gene name MIR168. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 32
Under cold stress, miR319 expression decreased at time points of 8, 12 and 24 h. The expression levels of miR319 normalized by different reference genes were not exactly the same (Fig.   5b); although the normalization results of U6 snRNA and miR168 were similar, the expression normalized by miR160e and miR160e +  miR168 showed no significant differences at most time points. [score:7]
For drought stress, when normalized by miR168 and the others, the expression of target gene miR408 showed disagreement. [score:5]
Because of the involvement in fundamental physiological processes, the stable expression of miR160e and miR168 was a reasonable result. [score:3]
Considering the target gene was miRNA, and miR168 was the top 1 reference gene of comprehensive ranking under drought stress, we suggest miR168 as the reference gene for miRNA qRT-PCR, rather than the others. [score:3]
For miR168, the function is regulation of the ARGONAUTE1 protein [53], which is the primary component of the RNA -induced slicing complex (RISC) and influences plant growth and development 54, 55. [score:3]
Therefore, the optimal reference gene sets under salinity and cold stress were miR160e +  miR164a and miR160e +  miR168, respectively. [score:1]
The rank of mean CV values of candidate reference genes was U6 snRNA <  miR168 <  miR160e <  EF1 < miR164a <  ACT <  GAPDH <  5.8S rRNA <  UBQ, which represented the fluctuation difference of Cq values. [score:1]
Significant differences occurred at all time points except for d 0 and were primarily between miR168 and the other reference genes. [score:1]
According to the miRNA high-throughput sequencing results in grapevine 28, 60 and reference gene selection research for miRNA qRT-PCR in other plant species 27, 29, 32, miR156a, miR159a, miR160e, miR162, miR164a, miR167a, miR168, miR169a, and miR396a were chosen as potential normalization factors for miRNA qRT-PCR in grapevine. [score:1]
miR160e, miR164a and miR168 were ranked at the top one or top two under cold, salinity and drought stresses, indicating these genes were more stable than protein-coding genes and 5.8S rRNA. [score:1]
According to geNorm, miR160e +  miR164a, miR160e +  miR168, and ACT +  UBQ +  GAPDH were selected as the most suitable reference gene sets for salinity, cold and drought stresses, respectively. [score:1]
U6 snRNA and miR164a, U6 snRNA and miR160e, and miR168 and UBQ were the candidate genes ranked at the top 2 under salinity, cold and drought stress, respectively, whereas the least stable candidate genes under these stresses were UBQ and 5.8S rRNA, miR164a and UBQ, and miR164a and U6 snRNA, respectively. [score:1]
Therefore, to guarantee a comprehensive comparison, the candidate reference genes of this research included traditional housekeeping genes (ACT, UBQ, GAPDH and EF1), wi dely used reference genes in miRNA qRT-PCR (5.8S rRNA and U6 snRNA), and conserved miRNAs (miR160e, miR164a and miR168). [score:1]
In conclusion, in grapevine, U6 snRNA, miR164a, miR160e and miR160e + miR164a are all suitable reference genes or gene set under salinity stress, whereas U6 snRNA, miR160e, miR168 and miR160e +miR168 can be used for qRT-PCR normalization under cold stress. [score:1]
However, UBQ and miR168 were indicated as the top 2 most stable genes by at least two algorithms. [score:1]
Based on the rankings and V values, which were generated by RankAggreg and geNorm, respectively, the reference genes and gene set were selected as follow: U6 snRNA, miR164a, miR160e, and miR160e + miR164a for salinity stress; U6 snRNA, miR160e, miR168, and miR160e +miR168 for cold stress; miR168, UBQ, EF1, and ACT +  UBQ +  GAPDH for drought stress. [score:1]
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2
[+] score: 19
For example, in GRSPaV-free grapevines, miR166 and miR396 were down-regulated by drought, similar to their reported expression in O. Sativa 37; miR168 was up-regulated in Arabidopsis thaliana, in agreement with its response to ABA-inducing stresses 16 and miR156, miR167 and miR397 were not affected by drought in grapevine, thus confirming their species-specific drought response 35 38. [score:9]
In infected plants, miR166, miR319, miR396, miR3631, mi3633 and miR3639 were down-regulated in SWS, whereas miR168, miR482, miR535, miR2111, miR3624 and miR3634 were up-regulated (Fig. 2, Supplementary Fig. S4). [score:7]
Indeed, in WW conditions, most conserved miRNAs that were validated by qRT-PCR (miR164, miR168, miR172, miR319, miR390, miR394, miR2111, miR3624, miR3629, miR3639) were expressed at higher levels in infected plants than in GRSPaV-free plants (Fig. 2, Supplementary Fig. S4). [score:3]
[1 to 20 of 3 sentences]
3
[+] score: 15
Other miRNAs from this paper: vvi-MIR156a, vvi-MIR156b, vvi-MIR156c, vvi-MIR156d, vvi-MIR156e, vvi-MIR156f, vvi-MIR156g, vvi-MIR156i, vvi-MIR159a, vvi-MIR159c, vvi-MIR160a, vvi-MIR160b, vvi-MIR160c, vvi-MIR160d, vvi-MIR160e, vvi-MIR162, vvi-MIR164a, vvi-MIR164b, vvi-MIR164c, vvi-MIR164d, vvi-MIR166a, vvi-MIR166b, vvi-MIR166c, vvi-MIR166d, vvi-MIR166e, vvi-MIR166f, vvi-MIR166g, vvi-MIR166h, vvi-MIR167a, vvi-MIR167b, vvi-MIR167c, vvi-MIR167d, vvi-MIR167e, vvi-MIR169a, vvi-MIR169y, vvi-MIR169c, vvi-MIR169d, vvi-MIR169e, vvi-MIR169f, vvi-MIR169g, vvi-MIR169j, vvi-MIR169k, vvi-MIR169m, vvi-MIR169p, vvi-MIR169r, vvi-MIR169s, vvi-MIR169t, vvi-MIR169u, vvi-MIR171a, vvi-MIR171b, vvi-MIR171c, vvi-MIR171d, vvi-MIR171e, vvi-MIR171f, vvi-MIR171h, vvi-MIR171i, vvi-MIR172a, vvi-MIR172b, vvi-MIR172c, vvi-MIR172d, vvi-MIR319b, vvi-MIR319c, vvi-MIR319f, vvi-MIR319g, vvi-MIR393b, vvi-MIR394a, vvi-MIR394b, vvi-MIR395a, vvi-MIR395b, vvi-MIR395c, vvi-MIR395d, vvi-MIR395e, vvi-MIR395f, vvi-MIR395g, vvi-MIR395h, vvi-MIR395i, vvi-MIR395j, vvi-MIR395k, vvi-MIR395l, vvi-MIR395m, vvi-MIR396a, vvi-MIR396b, vvi-MIR396d, vvi-MIR398a, vvi-MIR399a, vvi-MIR399b, vvi-MIR399e, vvi-MIR399g, vvi-MIR399h, vvi-MIR408, vvi-MIR479, vvi-MIR535a, vvi-MIR535b, vvi-MIR535c, vvi-MIR156h, vvi-MIR169b, vvi-MIR169h, vvi-MIR169i, vvi-MIR169l, vvi-MIR169n, vvi-MIR169o, vvi-MIR169q, vvi-MIR169v, vvi-MIR169w, vvi-MIR169x, vvi-MIR171g, vvi-MIR319e, vvi-MIR393a, vvi-MIR394c, vvi-MIR395n, vvi-MIR396c, vvi-MIR397a, vvi-MIR398b, vvi-MIR398c, vvi-MIR399c, vvi-MIR399d, vvi-MIR399f, vvi-MIR399i, vvi-MIR403a, vvi-MIR403b, vvi-MIR403c, vvi-MIR403d, vvi-MIR403e, vvi-MIR403f, vvi-MIR477a, vvi-MIR482, vvi-MIR828a, vvi-MIR845a, vvi-MIR845b, vvi-MIR845c, vvi-MIR845d, vvi-MIR845e, vvi-MIR477b, vvi-MIR171j
Vaucheret et al. [49] showed that AGO1, the target of miR168 is involved in the regulation of miR168 stability. [score:4]
Many predicted precursors show a wide expression (miR156d, miR159c, miR166a and c, miR168, miR171a, miR398a, miR398b and c, miR408, miR482). [score:3]
Figure 4C shows evidence for expression of the miR168 locus. [score:3]
Panel A refers to miR394B, panel B to miR162 and panel C to miR168. [score:1]
For several pre/pri-miRNA loci (notably miR162 and miR168) we infer several closely related canonical introns (shared splice donors with splice acceptor sites shifted by a few tens of bases or vice-versa). [score:1]
Supplemental Figure S5: Splice junction read coverage for Vvi-miR394b, Vvi-miR162 and Vvi-miR168. [score:1]
For miR162 and miR168, this hypothesis might be consistent with the low levels of mature microRNA observed by deep-sequencing, in contrast to the apparently high spliced transcript levels. [score:1]
Some families (notably miR168) show a greatly exaggerated tendency towards sequence errors with 13,477 short sequences showing a single substitution (G in the genomic sequence, A in the reads recovered) from the predicted sequence and not exhibiting satisfactory alternative map positions on the grapevine genome (not shown). [score:1]
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4
[+] score: 14
Vaucheret et al. [50] showed that AGO1, the target of miR168 is involved in the regulation of miR168 stability. [score:4]
Many predicted precursors show a wide expression (miR156d, miR159c, miR166a and c, miR168, miR171a, miR398a, miR398b and c, miR408, miR482). [score:3]
Figure 3C shows evidence for expression of the miR168 locus. [score:3]
Supplemental Figure S4: Splice junction read coverage for Vvi-miR394b, Vvi-miR162 and Vvi-miR168. [score:1]
For several pre/pri-miRNA loci (notably miR162 and miR168) we infer several closely related canonical introns (shared splice donors with splice acceptor sites shifted by a few tens of bases or vice-versa). [score:1]
Panel A refers to miR394B, panel B to miR162 and panel C to miR168. [score:1]
For miR162 and miR168, this hypothesis might be consistent with the low levels of mature microRNA observed by deep-sequencing, in contrast to the apparently high spliced transcript levels. [score:1]
[1 to 20 of 7 sentences]
5
[+] score: 3
Nine miRNA families (miR164, miR166, miR167, miR168, miR169, miR395, miR3623, miR3633, and miR3636) were strongly expressed, with more than 10,000 reads detected in at least one of the two libraries. [score:3]
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