8 publications mentioning vvi-MIR166c

Open access articles that are associated with the species Vitis vinifera and mention the gene name MIR166c. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

For example, in GRSPaV-free grapevines, miR166 and miR396 were down-regulated by drought, similar to their reported expression in O. Sativa 37; miR168 was up-regulated in Arabidopsis thaliana, in agreement with its response to ABA-inducing stresses 16 and miR156, miR167 and miR397 were not affected by drought in grapevine, thus confirming their species-specific drought response 35 38.

In infected plants, miR166, miR319, miR396, miR3631, mi3633 and miR3639 were down-regulated in SWS, whereas miR168, miR482, miR535, miR2111, miR3624 and miR3634 were up-regulated (Fig. 2, Supplementary Fig. S4).

Our data suggest that the virus -induced changes in miRNA expression between WW and SWS conditions; in particular, the presence of GRSPaV, significantly affected the accumulation of miR156, miR164, miR166, miR394, miR396, and miR3639 in response to SWS (Fig. 2, Supplementary Fig. S4) and the relevance of this is discussed later.

Notably, most differences in miRNA accumulation between infected and GRSPaV-free plants disappeared in SWS conditions and significant differences in expression remained only for miR166, miR319, miR390, and miR3639 (Supplementary Fig. S4).

2

This high level of vvi-miR166 expression was also seen in a previous study where it was the most dominantly expressed miRNA family in all assayed grapevine tissues [26].

The vvi-miR166 family showed the highest levels of expression, but had no significant difference in terms of the total normalised read counts between the two different library types (S2 File).

3

Nine miRNA families (miR164, miR166, miR167, miR168, miR169, miR395, miR3623, miR3633, and miR3636) were strongly expressed, with more than 10,000 reads detected in at least one of the two libraries.

The medium miRNA families were miR395, miR156, miR166, and miR399, with 13, 9, 8, and 8 members, respectively.

4

Our analysis showed that in fact highly abundant (more than 1000 TP5M) miRNAs are similar in almost all the samples, but stamen and carpels, with vvi-miR166 family being by far the most abundant (above 30,000 TP5M and reaching 1 million TP5M), followed by vvi-miR3634-3p (a grapevine specific miRNA with unknown target), by vvi-miR159c.

Vvi-miR166c/d/e/f/g/h/i-3p abundance profile is noteworthy: this molecule is the most abundant in our sequenced libraries.

5

Prigge and Clark [73], and Floyd and Bowman [75] have previously suggested that HD-Zip III sequences across all land plants produce transcripts that could be targeted by miRNA165 and miRNA166.

6

For example miR171e transcripts are detected only in callus, miR171f is only transcribed in stem while miR171 g is observed in callus and root - a similar situation can be observed for several families including miR166, miR167 and miR169).

For some families, thousands or even hundreds of thousands of short RNA sequences were recovered (miR156, miR164, miR166, miR167, miR172, miR393, miR396), while less than 1000 sequences corresponded to each of the remaining represented families.

In some cases, the most commonly observed sequences were identical to at least one of the predicted mature sequences (notably: miR156, miR160, miR164, miR167, miR169, miR172, miR394, miR399) while for other families, the predominant mature miRNA sequenced exhibited small variations (shifts or differences of length of one or two bases) with respect to the predicted mature sequences (e. g. miR166, miR393, miR395, miR396, miR408).

7

Lanes 1'-20' are 3'RACE (C) and 5'RACE (D) products of 20 higher abundance va-miRNAs (va-miR156e, va-miR160c, va-miR162, va-miR164c, va-miR166c, va-miR169m, va-miR171c, va-miR172c, va-miR408, va-miR535a, va-miR001, va-miR007, va-miR016, va-miR018, va-miR023, va-miR046, va-miR047, va-miR049, va-miR057 and va-miR062, respectively).

Although the va-miR169 family had the highest number of miRNA members with SNPs, the va-miR166 family topped in the list of total number of SNPs, followed by va-miR156, va-miR167 and va-miR169 families (Figure 3).

8

For example miR171e transcripts are detected only in callus, miR171f is only transcribed in stem while miR171g is observed in callus and root - a similar situation can be observed for several families including miR166, miR167 and miR169).