5 publications mentioning vvi-MIR164b

Open access articles that are associated with the species Vitis vinifera and mention the gene name MIR164b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

Relative expression levels of miR396, miR156, and miR164 and their respective targets in Grapevine rupestris stem pitting -associated virus(GRSPaV)-free and infected ‘Bosco’ leaves as determined by qRT-PCR.

The VvNAC05 (VIT_17s0000g06400 30) gene is a target of miR164 24 and showed a negative correlation with the expression of miR164 in the WW treatment (Fig. 2).

For example, in infected grapevines, we observed that the regulation of some miRNAs and their targets involved in leaf development was influenced by virus infection (miR156, miR164, miR319, miR394, miR396; Fig. 2, Supplementary Fig. S4).

No clear correlation was observed between miR164 and its target gene expression in WS and SWS conditions.

The combination VvNAC11 (VIT_14s0108g01070)/miR s409712_2 showed the same regulation as VvNAC5/miR164, even though the increased expression of this NAC gene in response to WS was not as great as that observed for VvNAC05 (Fig. 3 vs.

Indeed, in WW conditions, most conserved miRNAs that were validated by qRT-PCR (miR164, miR168, miR172, miR319, miR390, miR394, miR2111, miR3624, miR3629, miR3639) were expressed at higher levels in infected plants than in GRSPaV-free plants (Fig. 2, Supplementary Fig. S4).

Our data suggest that the virus -induced changes in miRNA expression between WW and SWS conditions; in particular, the presence of GRSPaV, significantly affected the accumulation of miR156, miR164, miR166, miR394, miR396, and miR3639 in response to SWS (Fig. 2, Supplementary Fig. S4) and the relevance of this is discussed later.

In our experiments, we observed that VvNAC05, VvNAC11 and VvNAC17 (targets of miR164, miRs409712_2 and miRC102, respectively) were induced by water stress.

2

vvi-miR164 is also modulated during berry development (125.5 TP5M in berry at fruit set, then declines below the threshold of expression), confirming previous observations [24, 86] that show its gradual decrease during ripening.

vvi-miR164 target gene is VvNAC33 [28, 86], reported to increase during ripening [87].

Some of the most conserved miRNAs reveal intriguing profiles: among others, miR156 and miR164 seem to be involved in berry maturation, in an ethylene independent manner.

3

On the contrary miR164a, miR164b, miR171c and miR172c show a significantly lower level of expression in roots (Figure 3F).

For some families, thousands or even hundreds of thousands of short RNA sequences were recovered (miR156, miR164, miR166, miR167, miR172, miR393, miR396), while less than 1000 sequences corresponded to each of the remaining represented families.

In some cases, the most commonly observed sequences were identical to at least one of the predicted mature sequences (notably: miR156, miR160, miR164, miR167, miR169, miR172, miR394, miR399) while for other families, the predominant mature miRNA sequenced exhibited small variations (shifts or differences of length of one or two bases) with respect to the predicted mature sequences (e. g. miR166, miR393, miR395, miR396, miR408).

4

On the contrary miR164a, miR164b, miR171c and miR172c show a significantly lower level of expression in roots (Figure 2F).

5

Nine miRNA families (miR164, miR166, miR167, miR168, miR169, miR395, miR3623, miR3633, and miR3636) were strongly expressed, with more than 10,000 reads detected in at least one of the two libraries.