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7 publications mentioning hsa-mir-1305

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-1305. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 195
As shown in Figure 2F, overexpression of miR-1305 abolished the up-regulated expression of Sox9, miR-130a, as well as miR-145, which indicated the inhibition of DANCR-facilitated chondrogenesis. [score:10]
Our result demonstrated that highly expressed DANCR results in the decreased expression of miR-1305, which consequently negatively regulated the expression of Smad4. [score:8]
Figure 3Smad4 is a target of miR-1305(A) The candidate downstream targets of miR-1305 were predicted with the microRNAorg, PITA, and TargetScan databases. [score:7]
To confirm this observation, the expression abundance of miR-1305 with highly expressed DANCR was detected by real-time quantitative RT-PCR (RT-qPCR) and the result showed a significant decreased level of miR-1305 in DANCR -overexpressed SMSCs (Figure 2A). [score:7]
Notably, overexpression of miR-1305 suppressed the promotion of DANCR in the cell proliferation and chondrogenic differentiation of SMSC, suggesting the negative regulatory loop between DANCR and miR-1305. [score:6]
Figure 2DANCR negatively regulates the expression of miR-1305(A) SMSCs were transfected with the expression plasmid of DANCR or the control vector. [score:6]
And miR-1305 targetted and negatively regulated the expression of Smad4 in SMSCs. [score:6]
Our results showed that DANCR negative regulates the expression of miR-1305 and Smad4 is a major target of miR-1305. [score:6]
Overexpression of DANCR down-regulates miR-1305. [score:6]
The result showed that the mRNA level of Smad4 was not significantly changed, while the protein abundance of Smad4 was down-regulated in SMSCs with highly expressed miR-1305 (Figure 3C,D). [score:6]
Down-regulation of miR-1305 enhanced the chondrogensis of SMSCs through targetting Smad4. [score:6]
Consistently, down-regulation of DANCR increased the expression level of miR-1305 (Figure 2B). [score:6]
Further mechanism study uncovered that miR-1305 targetted and negatively regulated the expression of Smad4 in SMSCs. [score:6]
Consistent with this result, SMSCs were transfected with the miR-1305 antagomir to inhibit the endogenous expression of miR-1305. [score:5]
To search for the downstream targets of miR-1305, TargetScan (www. [score:5]
Consistently, highly expressed miR-1305 suppressed the promotion of DANCR in cell proliferation and chondrogenic differentiation of SMSCs. [score:5]
In the present study, we found that miR-1305 interacts with the 3′-UTR of Smad4; overexpression of miR-1305 led to a significant reduction in Smad4 protein expression. [score:5]
With miRNA microarray analysis, we found that the expression level of miR-1305 was significantly decreased in DANCR -overexpressed SMSCs. [score:5]
In the present study, we characterized the underlying molecular mechanism that overexpression of DANCR down-regulates miR-1305. [score:4]
The knockdown efficiency of DANCR (left panel) and the expression level of miR-1305 (right panel) were determined. [score:4]
These results demonstrated that up-regulation of miR-1305 antagonized the effect of DANCR in chondrogenesis. [score:4]
DANCR down-regulates miR-1305. [score:4]
DANCR negatively regulates the expression of miR-1305. [score:4]
As DANCR negatively regulates miR-1305, we hypothesized that whether highly expressed miR-1305 could reverse the promoting effect of DANCR on chondrogensis. [score:4]
Of the dysregulated miRNAs, miR-1305 was the most down-regulated miRNA in SMSCs harboring DANCR compared with the control. [score:4]
Recent study demonstrated that down-regulation of miR-1305 facilitated the maintenance of pluripotency and increased the cell survival of human induced pluripotent stem cells (hiPSCs) [37]. [score:4]
il) databases were used to predict the candidate targets of miR-1305. [score:3]
Smad4 is the target of miR-1305. [score:3]
In addition, the endogenous expression of miR-1305 was depleted by transfecting miR-1305 antagomir, and the protein level of Smad4 was detected by. [score:3]
This result suggested that Smad4 is a target of miR-1305. [score:3]
Smad4 is a target of miR-1305. [score:3]
Increased luciferase activity was observed with the suppression of miR-1305 (Figure 2E). [score:3]
These results identified Smad4 as a downstream target of miR-1305 in chondrogenic differentiation of SMSCs. [score:3]
To this end, SMSCs stably expressing DANCR were transfected with miR-1305 mimics and the chondrogenic differentiation was monitored. [score:3]
In conclusion, the present study demonstrated that miR-1305 was significantly decreased in SMSCs with the overexpression of DANCR. [score:3]
To further illustrate the regulatory relationship between DANCR and miR-1305, we detected the binding between DANCR and miR-1305. [score:2]
Collectively, DANCR regulated miR-1305-Smad4 axis in the cell proliferation and chondrogenic differentiation of SMSCs. [score:2]
The relative expression abundance of miR-1305 was detected with RT-PCR assay. [score:2]
These results indicated the negative regulatory relationship between miR-1305 and Smad4. [score:2]
These results point to an important role for miR-1305 as a novel regulator of cell differentiation and proliferation. [score:2]
These data indicated the DANCR regulated miR-1305-Smad4 axis in chondrogenic differentiation (Figure 4G). [score:2]
The result showed that the luciferase activity was significantly decreased in the presence of miR-1305, while transfection of the mutant pMIR-REPORT-DANCR with miR-1305 mimics had no obvious effect on the luciferase activity (Figure 2D). [score:1]
The binding between DANCR and miR-1305 was detected by PCR. [score:1]
As shown in Figure 3E, depletion of miR-1305 increased the protein level of Smad4. [score:1]
Consistently, cotransfection of DANCR with miR-1305 antagomir further enhanced the effect of DANCR in chondrogenic differentiation (Figure 2G). [score:1]
The relative expression level of miR-130a, miR-145, and miR-1305 were calculated with the 2–ΔΔ C [t] method. [score:1]
Depletion of miR-1305 with miR-1305 antagomir increased the luciferase activity (E); ** P<0.01, Student’s t test. [score:1]
The result showed that miR-1305 was detected in the immumocomplex enriched by DANCR, which suggested the interaction between DANCR and miR-1305 (Figure 2C). [score:1]
As shown in Figure 3B, wild-type Smad4 showed decreased luciferase activity in the presence of miR-1305, whereas the relative luciferase activity in the mutant 3′-UTR of Smad4 was comparable with that of the scramble control cells. [score:1]
This observation suggested that miR-1305 might serve as a downstream effector in DANCR-enhanced cell proliferation and chondrogenesis. [score:1]
To further confirm this conclusion, we investigated the protein and mRNA level of Smad4 with SMSCs expressing miR-1305. [score:1]
SMSCs were cotransfected with pMIR-REPORT-DANCR and miR-1305 mimics or the control vector. [score:1]
Significantly decreased luciferase activity was observed with the transfection of miR-1305 mimics (D). [score:1]
These results demonstrated the binding between miR-1305 and DANCR. [score:1]
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2
[+] score: 13
In basal conditions (day 0), up-regulated miRNAs were: miR-1225-3p, miR-1305, miR-1238, miR-425, miR-191* and miR-34a, while miR-378 was the only down-regulated miR. [score:7]
Five miRs have one single target gene (miR-202: CBFA2T3, miR-500: NEBL, miR-378: KIF26A, miR-892b: BACH2, miR-1305:ID1), with a restricted impact on gene expression modulation. [score:5]
Among these miRs only miR-1305 was maintained significantly modulated over osteoblast-like differentiation. [score:1]
[1 to 20 of 3 sentences]
3
[+] score: 4
Significantly deregulated miRNAs were miR-3163 (fold change 2.0, p = 0.05), miR-539 (fold change 0.5, p = 0.001), miR-1305 (fold change 0.5, p = 0.05), miR-1260 (fold change 0.5, p = 0.03) and let-7a (fold change 0.3, p = 0.02, in all cases unadjusted p-values; 30 most deregulated probes). [score:3]
Among the panel of miRNAs, there are some with relatively unknown cell cycle function, such as miR-3163, miR-1305, miR-1260. [score:1]
[1 to 20 of 2 sentences]
4
[+] score: 3
Five of the miRNAs (miR-320c, miR-320d, miR-365a-3p, miR-494, and miR-1305) did not have detectable expression when using the platform. [score:3]
[1 to 20 of 1 sentences]
5
[+] score: 2
Seven miR were selected for follow-up (supplementary table S7) including three miR that were differentially regulated in the N v TS comparison (miR-30c, miR-32, miR-203) and four from the N v TSI comparison (miR-10a, miR-147b, miR-1285, miR-1305). [score:2]
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6
[+] score: 1
Both miR-1305 and miR-3198 correlated with IGF-BP3 after training and miR-128-3p correlated with the free testosterone. [score:1]
[1 to 20 of 1 sentences]
7
[+] score: 1
The limiting of prediction mo dels to 15 and 10 miRNAs expectedly resulted in some loss of performance, but it also revealed several miRNAs (miR-140-5p, miR-199a-3p, miR-93, miR-652, miR-1305, miR-224, miR-96, miR-766) that consistently contributed to all mo dels. [score:1]
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