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10 publications mentioning rno-mir-500

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-500. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 76
To verify the reliability of the miRNA sequencing results, the most upregulated (miR-500-3p and miR-770-3p) and downregulated (miR-450a-5p and miR-196c-3p) miRNAs in the exosomes derived from old rats in comparison to those from young rats, were selected for further verification using quantitative real-time PCR (qRT-PCR). [score:7]
To understand more thoroughly how miR-500-3p and miR-770-3p may contribute to the aging and CR, we predicted their target genes using TargetScan and analyzed the biological processes, pathways, and protein classes associated with each of the miRNAs using the PANTHER database. [score:5]
miR-500-3p expression was significantly downregulated in cardiac tissues of 7-day-old mice compared to 1-and 6-day-old mice, which may have important implications for the myocardial regenerative process [23]. [score:5]
The result showed significant downregulation of miR-500-3p and miR-770-3p, which was significantly increased in old rats in comparison to young rats. [score:4]
Enrichment analysis for biological processes, pathways, and protein classes of target genes regulated by miR-500-3p and miR-770-3p. [score:4]
Among identified miRNAs, miR-500-3p and miR-770-3p, the most highly upregulated miRNAs with aging were significantly decreased by CR. [score:4]
Target genes and functional prediction of miR-500-3p and miR-770-3p. [score:3]
In particular, we found that the expression of miR-500-3p and miR-770-3p was significantly increased with aging, whereas these were decreased by CR. [score:3]
In addition, we have examined the expression of miR-500-3p and miR-770-3p in serum of young, old, and old-CR rats. [score:3]
Also, miR-500-3p showed a significantly increased expression in peripheral CD3 [+] T lymphocytes from susceptible mouse strain to collagen -induced arthritis (CIA) and these results imply miRNA that could be involved in inflammatory process following CIA induction [24]. [score:3]
ROC curves for classifying the serum exosomal miRNAs from young and old rats were produced using the expression values for (A) miR-500-3p and (B) miR-770-3p. [score:3]
Figure 4ROC curves for classifying the serum exosomal miRNAs from young and old rats were produced using the expression values for (A) miR-500-3p and (B) miR-770-3p. [score:3]
Therefore, miR-500-3p and miR-770-3p may similarly contribute to accelerate the age-related senescence of stem cells and proliferating cells as well as age -associated inflammatory diseases. [score:3]
Particularly, both miR-500-3p and miR-770-3p could mainly target the transcription factors that control the gene transcription and genes involved in the Wnt/chemokines and cytokines-related inflammatory signaling pathways. [score:3]
Figure 3A showed that the expression of miR-500-3p and miR-770-3p was significantly increased in the old group compared to that in the young group. [score:2]
Finally, using PANTHER analysis, we identified that both miR-500-3p and miR-770-3p might control the gene transcription through regulation of transcription factors and Wnt/chemokines and cytokines-related inflammation signaling pathways during aging. [score:2]
Therefore, expression of exosomal miR-500-3p and miR-770-3p in aged rat serum might present further possibilities as potential candidates for investigating the aging process. [score:1]
ROC curve analysis of miR-500-3p and miR-770-3p in the serum exosomes of young and old rats. [score:1]
The findings from our study provide the notion that miR-500-3p and miR-770-3p in serum exosomes of aging rats might be potential candidates to understand aging and CR. [score:1]
Therefore, we may need further investigation on miR-500-3p and miR-770-3p expression in human serum exosome to strengthen their potential role as aging biomarkers. [score:1]
Therefore, exosomal miR-500-3p and miR-770-3p could be potential biomarkers candidates to study aging. [score:1]
Therefore, further studies are required to clarify the cells or tissues of origin for miR-500-3p and miR-770-3p delivering exosomes and the detailed role of these two miRNAs in the aging process. [score:1]
Therefore, it suggests that miR-500-3p and miR-770-3p possess exosome specificity. [score:1]
Therefore, modulation of exosomal miR-500-3p and miR-770-3p by CR may underlie the anti-aging effects. [score:1]
Exosomal miR-500-3p and miR-770-3p as candidates for aging. [score:1]
We found that among analyzed miRNAs, miR-500-3p and miR-770-3p were modulated by aging and CR. [score:1]
There are limited previous published reports on miR-500-3p and miR-770-3p which were found in our study. [score:1]
These data suggests that exosomal miR-500-3p and miR-770-3p could be used to discriminate old rats from young rats. [score:1]
qRT-PCR was used to investigate the expression of (A) miR-500-3p, miR-770-3p, (B) miR-450a-5p, and miR-196c-3p. [score:1]
Figure 3qRT-PCR was used to investigate the expression of (A) miR-500-3p, miR-770-3p, (B) miR-450a-5p, and miR-196c-3p. [score:1]
Therefore, Figure 3 demonstrates that the qRT-PCR results for miR-500-3p, miR-770-3p, and miR-450a-5p were consistent with those of the RNA sequencing data. [score:1]
The area under the curve (AUC) for miR-500-3p and miR-770-3p were 0.823 and 0.826, respectively. [score:1]
This analysis suggests that miR-500-3p and miR-770-3p may be involved in similar biological functions in aging process. [score:1]
miR-500-3p and miR-770-3p, that were significantly changed in both aging and diet, were selected for further analysis. [score:1]
Furthermore, we analyzed the role of miR-500-3p and miR-770-3p in aging using PANTHER, a bioinformatics software. [score:1]
[1 to 20 of 35 sentences]
2
[+] score: 28
Other miRNAs from this paper: hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-27a, hsa-mir-30a, hsa-mir-32, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-93, hsa-mir-107, hsa-mir-129-1, hsa-mir-30c-2, hsa-mir-139, hsa-mir-181c, hsa-mir-204, hsa-mir-212, hsa-mir-181a-1, hsa-mir-222, hsa-mir-15b, hsa-mir-23b, hsa-mir-132, hsa-mir-138-2, hsa-mir-140, hsa-mir-142, hsa-mir-129-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-154, hsa-mir-186, rno-mir-324, rno-mir-140, rno-mir-129-2, rno-mir-20a, rno-mir-7a-1, rno-mir-101b, hsa-mir-29c, hsa-mir-296, hsa-mir-30e, hsa-mir-374a, hsa-mir-380, hsa-mir-381, hsa-mir-324, rno-mir-9a-1, rno-mir-9a-3, rno-mir-9a-2, rno-mir-15b, rno-mir-17-1, rno-mir-18a, rno-mir-19b-1, rno-mir-19b-2, rno-mir-19a, rno-mir-21, rno-mir-23a, rno-mir-23b, rno-mir-24-1, rno-mir-24-2, rno-mir-27a, rno-mir-29c-1, rno-mir-30e, rno-mir-30a, rno-mir-30c-2, rno-mir-32, rno-mir-92a-1, rno-mir-92a-2, rno-mir-93, rno-mir-107, rno-mir-129-1, rno-mir-132, rno-mir-138-2, rno-mir-138-1, rno-mir-139, rno-mir-142, rno-mir-146a, rno-mir-154, rno-mir-181c, rno-mir-186, rno-mir-204, rno-mir-212, rno-mir-181a-1, rno-mir-222, rno-mir-296, rno-mir-300, hsa-mir-20b, hsa-mir-431, rno-mir-431, hsa-mir-433, rno-mir-433, hsa-mir-410, hsa-mir-494, hsa-mir-181d, hsa-mir-500a, hsa-mir-505, rno-mir-494, rno-mir-381, rno-mir-409a, rno-mir-374, rno-mir-20b, hsa-mir-551b, hsa-mir-598, hsa-mir-652, hsa-mir-655, rno-mir-505, hsa-mir-300, hsa-mir-874, hsa-mir-374b, rno-mir-466b-1, rno-mir-466b-2, rno-mir-466c, rno-mir-874, rno-mir-17-2, rno-mir-181d, rno-mir-380, rno-mir-410, rno-mir-598-1, rno-mir-674, rno-mir-652, rno-mir-551b, hsa-mir-3065, rno-mir-344b-2, rno-mir-3564, rno-mir-3065, rno-mir-1188, rno-mir-3584-1, rno-mir-344b-1, hsa-mir-500b, hsa-mir-374c, rno-mir-29c-2, rno-mir-3584-2, rno-mir-598-2, rno-mir-344b-3, rno-mir-466b-3, rno-mir-466b-4
These miRNAs were chosen as representative of the different patterns that were observed: up-regulation (miR-21-5p) or down-regulation (miR-222-3p) during latency; up-regulation (miR-181c-5p) or down-regulation (miR-500-3p) in the chronic period; up-regulation (miR-146a-5p) or down-regulation (miR-551b-3p) in the entire course of the disease. [score:21]
Some miRNAs (miR-129-1-3p; miR-129-2-3p, miR-129-5p, miR181c-5p, miR181d-5p, miR-409a-5p, miR-655 and miR-874-3p) were up-regulated (Fig. 2, Supplementary Fig. S3A), whereas others (miR-296-5p, miR-500-3p and miR-652-3p) were down-regulated only in the chronic phase, while not being significantly altered during latency (Fig. 2, Supplementary Fig. S3B). [score:7]
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3
[+] score: 8
Of these, miR-500-3p, miR-23b-3p, miR-200a-3p, miR-19b-3p, miR-92a-1-5p, miR-21-5p, miR-21-3p, miR-1843-3p, miR-223-3p, miR-3473, and miR-129-2-3p were found to be upregulated, whereas miR-92b-3p, miR-3102, and miR-3577 were found to be downregulated in the rat brain. [score:7]
Nevertheless, many of them had not been reported (miR-500-3p, miR-1843-3p, and miR-3473, miR-3102, and miR-3577). [score:1]
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4
[+] score: 7
stress downregulated abundance of miR-329, miR-380, miR-20a, and miR-500 (all p≤0.05; Figure 2B-C). [score:4]
C, Table of target genes for miRNAs modulated by gestational stress (miR-329, miR-380, miR-20a, and miR-500; p≤0.05), and their physiological implications. [score:3]
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5
[+] score: 7
miR-448, let-7b, miR-540, miR-296, miR-880, miR-200a, miR-500, miR-10b, miR-336, miR-30d, miR-208, let-7e, miR-142-5p, miR-874, miR-375, miR-879, miR-501, and miR-188 were upregulated, while miR-301b, miR-134, and miR-652 were downregulated in TMH group (Table 5). [score:7]
[1 to 20 of 1 sentences]
6
[+] score: 6
There was no significant difference in the expression of rno-miR-500-3p, rno-miR-877, and rno-miR-3577 between RDE group and OVX group. [score:3]
Name Primers U6F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ rno-miR-500-3pGSP: 5′-GGAAGGCACCTGGGCAAG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-499-3pGSP: 5′-GGGGAACATCACAGCAAGTC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-214-3pGSP: 5′-GGGGACAGCAGGCACAGAC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-20b-5pGSP: 5′-GGGGCAAAGTGCTCATAGTG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-877GSP: 5′-GGGGAAGTAGAGGAGATGGC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-451-5pGSP: 5′-GGGGGAAACCGTTACCATTAC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-3577GSP: 5′-GGGTTCTGTCCCTCTTGGC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-370-3pGSP: 5′-AGCCTGCTGGGGTGGAA-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-181d-5pGSP: 5′-GGGGCATTCATTGTTGTCG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-23b-3pGSP: 5′-GGGATCACATTGCCAGGG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-191a-5pGSP: 5′-GGCAACGGAATCCCAAAAG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-200c-3pGSP: 5′-GGGGTAATACTGCCGGGTAA-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-328a-3pGSP: 5′-AACTCGCCCTCTCTGCCC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ nutrients-07-01333-t002_Table 2 Table 2 Primers of mRNA targets. [score:1]
In nearly all cases, the results of the qRT-PCR analysis generally agreed with the changes in the microarray analysis, with the exception of rno-miR-500-3p, rno-miR-877, and rno-miR-3577. [score:1]
Name Primers U6F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ rno-miR-500-3pGSP: 5′-GGAAGGCACCTGGGCAAG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-499-3pGSP: 5′-GGGGAACATCACAGCAAGTC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-214-3pGSP: 5′-GGGGACAGCAGGCACAGAC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-20b-5pGSP: 5′-GGGGCAAAGTGCTCATAGTG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-877GSP: 5′-GGGGAAGTAGAGGAGATGGC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-451-5pGSP: 5′-GGGGGAAACCGTTACCATTAC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-3577GSP: 5′-GGGTTCTGTCCCTCTTGGC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-370-3pGSP: 5′-AGCCTGCTGGGGTGGAA-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-181d-5pGSP: 5′-GGGGCATTCATTGTTGTCG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-23b-3pGSP: 5′-GGGATCACATTGCCAGGG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-191a-5pGSP: 5′-GGCAACGGAATCCCAAAAG-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-200c-3pGSP: 5′-GGGGTAATACTGCCGGGTAA-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ rno-miR-328a-3pGSP: 5′-AACTCGCCCTCTCTGCCC-3′ R: 5′-GTGCGTGTCGTGGAGTCG-3′ nutrients-07-01333-t002_Table 2 Table 2 Primers of mRNA targets. [score:1]
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7
[+] score: 3
After the FO diet, miR-380–5p, miR-27a-3p, and miR-500–3p expressions decreased compared with the SO diet. [score:2]
miR-500 was induced by OO diet. [score:1]
[1 to 20 of 2 sentences]
8
[+] score: 2
At 24 hours, five miRNAs (rno-miR-214, rno-miR-99a, rno-miR-363*, rno-miR-100 and rno-miR-340–5p) and at 48 hrs 6 miRNAs (rno-miR-34b, rno-miR-500, rno-miR-24-1*, rno-miR-29b, rno-miR-199a-3p, rno-let-7a) showed the most prominent dysregulation (P < 0.001) (Fig.   7B). [score:2]
[1 to 20 of 1 sentences]
9
[+] score: 1
The study by Yuuki Genda et al. also revealed that certain miRNAs, including miR-500, -221 and -21, were related to neuropathic pain in a chronic constriction injury rat mo del [31]. [score:1]
[1 to 20 of 1 sentences]
10
[+] score: 1
For example, seven microRNAs, including miR-1, miR-200c, miR-340, miR-342, miR-325, miR-139 and miR-500 contributed to the association with heart failure (P-value = 2.74e-3). [score:1]
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