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11 publications mentioning hsa-mir-941-1

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-941-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 56
We postulated that down-regulation of these predicted targets by ectopic expression of miR-941 and miR-1247 would negatively impact cell migration. [score:8]
We found that over -expression of miR-941 was able to significantly inhibit cell migration in both HCT116 and D KO cells and additionally in DLD1 cells. [score:5]
To verify that the up-regulation of these miRNAs was consequent to DNA demethylation after 5-aza-dC treatment, we assessed changes in DNA methylation status in the proximal regions of miR-941-1/3, miR-1237 and miR-1247 by bisulfite genomic sequencing (Figure 2). [score:4]
For instance, matrix metallopeptidase 24 (MMP24) is a predicted target for miR-941, and it facilitates tissue remo deling and cell migration in endometrium from endometriosis patients [36]. [score:3]
Finally, similar suppressive effects on cell migration were independently observed in DLD1 cells (Figure S4C) and D KO cells (Figure S5C) transfected with miR-941 and miR-1247 mimics. [score:3]
This result is reasonable considering several high priority predicted targets of miR-941 are related to cell migration. [score:3]
Over -expression of miR-941, miR-1237, and miR-1247 in HCT116, D KO, and DLD1 cells was achieved by transfecting the cells with 20 nM miRNA duplexes that mimicked the mature miR-941 (MSY0004984, Qiagen), miR-1237 (MSY0005592, Qiagen), and miR-1247 (MSY0005899, Qiagen). [score:3]
Hence, we tested the effects of ectopic expression of miR-941, miR-1237, and miR-1247 on cell mobility in HCT116 cells. [score:3]
To determine if expression of these epigenetically silenced miRNAs would affect cancer cell growth and migration, we transfected HCT116 cells with miRNA mimics of miR-941 (the mature form of miR-941-1 and -3), miR-1237, miR-1247, and a non-coding negative control (AllStars Neg. [score:3]
Furthermore, computationally predicted targets of miR-941 and miR-1247 included several genes involved in cell migration and invasion. [score:3]
miR-941 and miR-1247 suppress cell growth and migration in colon cancer cells. [score:3]
We focused on two important aspects of cancer development, growth and migration, and selected miR-941, miR-1237, and miR-1247 for functional studies. [score:2]
Functional analysis of miR-941, miR-1237, and miR-1247 in HCT116 cells. [score:1]
), miR-941, or miR-1247 mimics. [score:1]
HCT116 cells transfected with miR-941 and miR-1247 mimics and the AllStars Negative Control were re-plated on coverslips at 24 hr post-transfection. [score:1]
), miR-941, miR-1237, or miR-1247 mimics. [score:1]
was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells. [score:1]
), miR-941, or miR-1247. [score:1]
Based on qRT-PCR results, we determined that miR-1237, miR-602, miR-941-1, miR-941-3, and miR-663b are transcriptionally independent from their respective putative host genes (Figure 4). [score:1]
UCSC genome browser screen captures of the promoter regions (±1000 bp from annotated transcription start site) for (A) RPS6KA4, putative host gene for miR-1237, (B) C9orf3, putative host gene for miR-24-1 and miR-27b, (C) AB058779, putative host gene for miR-602, (D) ANKRD30BL, putative host gene for miR-663b, and (E) DNAJC5, putative host gene for miR-941-1 and miR-941-3. The first track contains DNA methylation at these promoters in HCT116, and the second track contains DNA methylation in D KO cells as detected by MiGS. [score:1]
Taken together, these observations suggest that the epigenetic silencing of miR-941 and miR-1247 may facilitate colon cancer cell migration and invasion. [score:1]
0020628.g002 Figure 2Bisulfite sequencing was carried out for (A) miR-941, (B) miR-1237, and (C) miR-1247 in parental HCT116 cells, HCT116 cells treated with 5 µM 5-aza-dC for 48 hr, and D KO cells. [score:1]
Figure S4 Functional analysis of miR-941, miR-1237, and miR-1247 in DLD1 cells. [score:1]
Our functional study of miR-941 substantiated this hypothesis. [score:1]
Figure S5 Functional analysis of miR-941 and miR-1247 in D KO cells. [score:1]
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2
[+] score: 13
Other miRNAs from this paper: hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-941-5
Also, mir-941 targets genes shown to be involved in insulin pathways and neurotransmitter signaling [80]. [score:3]
These evidences indicate that allelic differences in rs2427556 of mir-941-1 and HDAC2 might play important roles in cognitive impairment associated diabetes mellitus, also known as type 3 diabetes and was observed in cases of Alzheimer’s disease [81]. [score:3]
Positive selection signature in miR-941 of Asian populations. [score:1]
S24 FigWe identified rs2427556, located in the miR complementary region of miR-941-1 in this cluster (shown by dashed box in C), to be positive for the signature of selection based on F [ST] differences. [score:1]
Out of 65 variants, rs2427556 of has-miR-941-1 present in chromosome 20, showed high F [ST] differences between Asian and European populations (0.406 for CHB-CEU). [score:1]
Recently, it has been reportedthat mir-941 emerged de novo in humans from an evolutionarily volatile tandem repeat sequence, and multiple copies of this miRNA are present in the human genome [80]. [score:1]
Although we have not observed continental specific differences for variants rs7360929 of mir-941-3, we did observe that, after scanning100 kb of the flanking region,the miR-941 region showed selection signatures in the Asian populations (shown in red color) while the flanking regions did not. [score:1]
These variants are rs4809383, which is present in the stem region of mir-941-1 and rs7320929, which is present in mir-941-3. The allele frequency of rs2427556 was much higher in Asian populations (65% in CHB) than in European populations (15% in CEU), while rs4809383 was nearly the same in worldwide populations (S24A– S24C Figs). [score:1]
Two other variants were detected: rs4809383 is present in the stem region of mir-941-1 and rs7320929 is present in mir-941-3. (B) The allele frequency of rs2427556 was higher in Asian populations than in European populations, while rs4809383 has nearly the same frequency in worldwide populations. [score:1]
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3
[+] score: 7
Conversely, expression of MIR941 (50%, p < 0.05) and MIR589-5P (69%, p < 0.05), which were predicted by RNA-Seq analysis to be down regulated, were not validated by qPCR. [score:4]
We used qPCR to confirm expression patterns predicted by RNA-Seq analysis for 8 miRNA (MIR29A-5P, MIR140-5P, MIR126P, MIR374A-3P, MIR26A-2-3P and MIR147B, MIR941 and MIR589-5P) that were induced following treatment of any eCig liquid (ANY LIQUID). [score:3]
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4
[+] score: 6
For the efficient target VCAM1, miR-941 repressed it only in normal stage, thus it had contribution to the ‘regulation of immune response’ process only in CIN I stage. [score:4]
Our finding suggests that based on miR-941’s differential regulation on VCAM1, the leukocyte-endothelial cell adhesion may be promoted in CIN I stage, thus accelerating inflammation in local tissue. [score:2]
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5
[+] score: 5
The smallest Student t-test P-value for a miRNA for nonprogressed vs progressed data was α=0.0053 (miR-941) (see s and Methods). [score:1]
For example, the score 11 − 0 − 2 = 9 is reached for miR-199a-3p 5′-ACAGUAGUCUGCACAU_UGGUUA-3' and miR-941 5'-CACCCGGCUGUGUGCACAUGUGC-3′ because they contain the common subsequence GU-UGCACAU-UG (Supplementary Materials and Methods). [score:1]
[44]Restricting attention to the 40 most robustly expressed miRNAs (that include 3 miRNAs from equation (1), namely, miR-941, miR-103a-3p, and miR-92a-3p), we contrasted miRNA–miRNA correlation networks over the 3 groups by randomly selecting 1000 times subsets of 25 samples from each group and calculating all 780 Pearson correlations of pairs (from 40 miRNAs represented by 25-dimensional vectors). [score:1]
Notably, miR-941 and miR-103a-3p were both included in the correlation networks for unaffected and nonprogressed subjects but absent from the network for progressed subjects. [score:1]
[44] Restricting attention to the 40 most robustly expressed miRNAs (that include 3 miRNAs from equation (1), namely, miR-941, miR-103a-3p, and miR-92a-3p), we contrasted miRNA–miRNA correlation networks over the 3 groups by randomly selecting 1000 times subsets of 25 samples from each group and calculating all 780 Pearson correlations of pairs (from 40 miRNAs represented by 25-dimensional vectors). [score:1]
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6
[+] score: 4
Other miRNAs from this paper: hsa-mir-941-2, hsa-mir-941-3, hsa-mir-941-4, hsa-mir-941-5
This host gene is repressed by miR-941, as well as two direct interacting partners of the protein encoded by DNAJC5 (ref. [score:2]
Based on DNAJC5 and its partner's functions, miR-941 was suggested to participate in neurotransmitter signalling 60. [score:1]
A remarkable example is the human-specific miR-941 hosted by DNAJC5. [score:1]
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7
[+] score: 3
Four EV-enriched pre-miRNAs (MIR3661, MIR941-1, MIR1282 and a novel miRNA AC009065) identified in sMVs were highly expressed (>100 FPKM), of them MIR3661 (ENST00000577394) was shared with EpCAM-Exos. [score:3]
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8
[+] score: 3
Here, several miRNAs such as hsa-miR-941 (not detected in any array experiment, not detected in RT-qPCR, average read count of ~1000 reads using Illumina HiSeq sequencing) had expression levels differing several orders of magnitude between the miRBase microarrays and using HiSeq sequencing. [score:3]
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9
[+] score: 3
One recently investigated human-specific non-coding RNA, miR-941, is expressed in the brain and regulates genes involved in neurotransmitter signaling (Hu et al., 2012). [score:2]
Evolution of the human-specific microRNA miR-941. [score:1]
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10
[+] score: 1
mir-106a∼mir-363 designates mir-106a/mir-18b/mir-20b/mir-19b-2/mir-92a-2/mir-363 cluster and mir-941-1∼3 designates mir-941-1/mir-941-2/mir-941-3 cluster. [score:1]
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11
[+] score: 1
In addition, Yan et al. (2011) performed a genome-wide methylome analysis entailing deep sequencing of MBD (methylated DNA binding domain)-isolated DNA in HCT116 cells, and identified a number of methylated genes, including miR-941, miR-1237, and miR-1247. [score:1]
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