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miRBase |
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![]() 7 publications mentioning mmu-mir-465b-1Open access articles that are associated with the species Mus musculus and mention the gene name mir-465b-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: mmu-mir-201, mmu-mir-465a, mmu-mir-547, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-465d
B) Pri-miRNA FISH images of pachytene cells expressing miR-465 in Spo11 null and Brca1 [Δ11/Δ11] mutants (defective in XY silencing).
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S5 FigCombined DNA / pri-miRNA FISH for miR-465 shows expression of miR-465 from the autosomal, transgenic locus (arrows) but not from the X locus (arrowhead).
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We targeted the X-miRNA miR-465, present in six copies in cluster 4 (Fig 1D).
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MiR-465 expression pattern recapitulates that of cluster 4 miRNAs (Fig 1D).
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This proved to be the case: in the few Spo11 null and Brca1 pachytene cells in which the miR-465 locus, identified using DNA FISH, lay within a γH2AFX domain (Spo11 null: n = 2 out of 54 cells; Brca1 [Δ11/Δ11]: n = 2 out of 15 cells), no pri-miR-465 expression could be observed (Fig 2C).
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S4 FigPri-miRNA FISH shows that miR-465 is silent in wild type pachytene cells.
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Detection of pri-miR-465 by pri-miRNA FISH in miRBAC line 1 transgenic pachytene spermatocytes.
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Top: Four variants of the miR-465 gene are repeated in the genome at the XA7 locus (see Fig 1D).
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Pri-miRNA FISH shows that miR-465 is silent in wild type pachytene cells.
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For miR-465-specific pri-miRNA FISH, a mix of nine amino-allyl -modified oligonucleotides labeled with fluorolink Cy3 were used as probes (S1 Table).
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Pri-miR-465-specific oligonucleotide probes and BAC probes were used for RNA and DNA FISH respectively.
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We observed the same results using our miR-465-specific pri-miRNA FISH protocol on X-miRBAC line 1 (S5 Fig) (100%, n = 9).
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We subsequently repeated the analysis of cluster 4 X-miRNAs using our oligonucleotide RNA FISH approach that specifically detects pri-miRNAs for the six copies of miR-465.
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Global DNA alignment of the precursors of the miR-465 variants is shown.
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C) In some rare pachytene cell, the miR-465 locus is found underneath the silencing body that forms in Spo11 null and Brca1 [Δ11/Δ11] spermatocytes.
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In addition, we performed miR-465-specific pri-miRNA FISH in a second MSCI mutant, the Brca1 [Δ11/Δ11] mo del [17, 18].
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Other miRNAs from this paper: hsa-mir-26a-1, hsa-mir-26b, mmu-mir-141, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, mmu-mir-205, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-205, hsa-mir-200b, mmu-mir-290a, mmu-mir-291a, mmu-mir-292a, mmu-mir-293, mmu-mir-294, mmu-mir-295, mmu-mir-302a, hsa-mir-141, mmu-mir-200a, mmu-mir-26a-1, mmu-mir-26b, hsa-mir-200c, mmu-mir-200c, mmu-mir-26a-2, mmu-mir-199a-2, mmu-mir-199b, hsa-mir-200a, hsa-mir-302a, hsa-mir-26a-2, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, hsa-mir-372, rno-mir-26a, rno-mir-26b, rno-mir-141, rno-mir-199a, rno-mir-200c, rno-mir-200a, rno-mir-200b, rno-mir-205, rno-mir-290, rno-mir-291a, rno-mir-292, hsa-mir-429, mmu-mir-429, rno-mir-429, mmu-mir-463, mmu-mir-465a, mmu-mir-471, mmu-mir-367, mmu-mir-291b, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, mmu-mir-741, mmu-mir-743a, mmu-mir-743b, mmu-mir-871, mmu-mir-880, mmu-mir-881, mmu-mir-883a, mmu-mir-883b, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-878, rno-mir-743b, rno-mir-871, rno-mir-878, rno-mir-880, rno-mir-881, rno-mir-883, rno-mir-463, rno-mir-471, rno-mir-743a, hsa-mir-302e, hsa-mir-302f, rno-mir-293, rno-mir-294, rno-mir-295-1, rno-mir-465, rno-mir-3551, rno-mir-291b, rno-mir-3580, rno-mir-741, rno-mir-295-2, rno-mir-6325, mmu-mir-292b, mmu-mir-465d, mmu-mir-290b
Several miRNAs, such as members of the miR-183-96-182 cluster, miR-741, miR-881, miR-878, miR-743a, miR-743b, miR-465, miR-871, miR-880, are known to be expressed in mouse ESCs and were also expressed in rat PSCs; however, their function in pluripotency induction and regulation has not been directly shown 48– 50.
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In addition, we found that the rno-miR-novel-8 miRNA was located less than 10 kb from miR-871, mir-3580 and miR-465, which were upregulated in rat PSCs.
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A cluster of miRNAs, comprised of miR-463, miR-465, miR-471, miR-741, miR-743a, miR-743b, miR-871, miR-878, miR-880, miR-881, and miR-883, was located on the X chromosome and was expressed at a high level in rat PSCs according to the sequencing data.
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Other miRNAs from this paper: mmu-let-7g, mmu-let-7i, mmu-mir-132, mmu-mir-145a, mmu-mir-146a, mmu-mir-181a-2, mmu-mir-205, mmu-mir-143, mmu-mir-296, mmu-let-7d, mmu-mir-130b, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-20a, mmu-mir-21a, mmu-mir-26a-1, mmu-mir-26b, mmu-mir-29c, mmu-mir-92a-2, mmu-mir-93, mmu-mir-34a, mmu-mir-17, mmu-mir-25, mmu-mir-212, mmu-mir-181a-1, mmu-mir-26a-2, mmu-mir-92a-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-181b-2, mmu-mir-434, mmu-mir-465a, mmu-mir-592, mmu-mir-743a, mmu-mir-181d, mmu-mir-743b, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-145b, mmu-mir-21b, mmu-let-7j, mmu-mir-21c, mmu-let-7k, mmu-mir-465d
Several members of the miR-465 and miR-743 families were up-regulated in both young and aged df/df mice, although they were expressed at lower levels than the previously discussed miRNAs.
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miR-465 was previously detected as one of the most highly expressed miRNAs in the newborn mouse ovary, despite being undetectable in the adult ovary [6].
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The mir-465 cluster is located in the X chromosome, and is strongly expressed in the testis, although not detected in other tissues [6].
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Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-101a, mmu-mir-125a, mmu-mir-133a-1, mmu-mir-141, mmu-mir-142a, mmu-mir-145a, mmu-mir-146a, mmu-mir-150, mmu-mir-183, mmu-mir-184, mmu-mir-199a-1, mmu-mir-200b, mmu-mir-206, mmu-mir-143, mmu-mir-291a, mmu-mir-200a, mmu-mir-18a, mmu-mir-21a, mmu-mir-31, mmu-mir-96, mmu-mir-34a, mmu-mir-328, mmu-mir-1a-2, mmu-mir-214, mmu-mir-223, mmu-mir-199a-2, mmu-mir-133a-2, mmu-mir-133b, mmu-mir-429, mmu-mir-216b, mmu-mir-671, mmu-mir-496a, mmu-mir-449c, mmu-mir-696, mmu-mir-18b, mmu-mir-465b-2, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-1b, mmu-mir-466f-4, mmu-mir-1903, mmu-mir-1936, mmu-mir-1306, mmu-mir-1947, mmu-mir-669o, mmu-mir-133c, mmu-mir-145b, mmu-mir-21b, mmu-mir-21c, mmu-mir-496b, mmu-mir-142b
Seven of the up-regulated miRNAs (miR-465b, miR-466f, miR-669o, miR-18b, miR-291a, miR-696, miR-101a) were also much more (≥ 5 fold) expressed in the retina compared to the sclera suggesting that they might be involved in the regulation of retina-specific processes.
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Several miRNAs exhibited more than 10-fold change in expression in the myopic retina (Table 1), including mmu-miR-1947-5p (FC = 31.5, p = 1.47 × 10 [−04]), mmu-miR-200a-5p (FC = 18.8, p = 9.46 × 10 [−05]), mmu-miR-141-5p (FC = 13.9, p = 4.75 × 10 [−06]), mmu-miR-465b-5p (FC = 12.8, p = 5.93 × 10 [−04]), mmu-miR-214-5p (FC = 12.6, p = 8.27 × 10 [−03]), mmu-miR-1936 (FC = 12.3, p = 9.56 × 10 [−06]), mmu-miR-466f-5p (FC = 11.5, p = 3.85 × 10 [−03]), mmu-miR-669o-5p (FC = 10.9, p = 2.18 × 10 [−03]), mmu-miR-18b-5p (FC = 10.1, p = 1.79 × 10 [−03]), and mmu-miR-145-5p (FC = -10.5, p = 8.87 × 10 [−09]).
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Other miRNAs from this paper: mmu-mir-207, mmu-mir-290a, mmu-mir-292a, mmu-mir-298, mmu-mir-15a, mmu-mir-16-1, mmu-mir-465a, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-292b, mmu-mir-465d, mmu-mir-290b
For instance, mmu-miR-290 was up-regulated in all organs except forestomach; mmu-miR-465 was down-regulated in lung, spleen and glandular stomach.
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Other miRNAs from this paper: mmu-mir-152, mmu-mir-465a, mmu-mir-743a, mdo-mir-152, mmu-mir-743b, mmu-mir-880, mmu-mir-883b, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-465d
The critical region also contains the Mir465 cluster of miRNA genes, which show a significant difference in expression between reciprocal F1 hybrids.
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Significant overexpression of Mir465 cluster in PWD germ cells is shown in red.
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Only the Mir465 [PWD] cluster displayed a significant increase of expression in the first meiotic prophase of PWD and B6 sorted testicular cells (Figure 3A, B).
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Other miRNAs from this paper: mmu-mir-30a, mmu-mir-30b, mmu-mir-141, mmu-mir-151, mmu-mir-10b, mmu-mir-191, mmu-mir-143, mmu-mir-30e, mmu-mir-34c, mmu-mir-34b, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-21a, mmu-mir-10a, mmu-mir-139, mmu-mir-375, mmu-mir-196b, mmu-mir-465a, mmu-mir-466a, mmu-mir-467a-1, mmu-mir-669a-1, mmu-mir-669b, mmu-mir-669a-2, mmu-mir-669a-3, mmu-mir-467b, mmu-mir-669c, mmu-mir-465b-2, mmu-mir-465c-1, mmu-mir-465c-2, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-467c, mmu-mir-467d, mmu-mir-466d, mmu-mir-208b, mmu-mir-467e, mmu-mir-466l, mmu-mir-669k, mmu-mir-669g, mmu-mir-669d, mmu-mir-466i, mmu-mir-669j, mmu-mir-669f, mmu-mir-669i, mmu-mir-669h, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-467f, mmu-mir-466j, mmu-mir-669e, mmu-mir-467g, mmu-mir-467h, mmu-mir-669l, mmu-mir-669m-1, mmu-mir-669m-2, mmu-mir-669o, mmu-mir-669n, mmu-mir-466m, mmu-mir-669d-2, mmu-mir-466o, mmu-mir-467a-2, mmu-mir-669a-4, mmu-mir-669a-5, mmu-mir-467a-3, mmu-mir-466c-2, mmu-mir-669a-6, mmu-mir-467a-4, mmu-mir-466b-4, mmu-mir-669a-7, mmu-mir-467a-5, mmu-mir-466b-5, mmu-mir-669p-1, mmu-mir-467a-6, mmu-mir-669a-8, mmu-mir-466b-6, mmu-mir-669a-9, mmu-mir-467a-7, mmu-mir-466b-7, mmu-mir-669p-2, mmu-mir-467a-8, mmu-mir-669a-10, mmu-mir-467a-9, mmu-mir-669a-11, mmu-mir-467a-10, mmu-mir-669a-12, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, mmu-mir-466q, mmu-mir-6240, mmu-mir-30f, mmu-mir-465d, mmu-mir-466c-3
Prominent among these were members of the let7, miR-30, miR-465, miR-466, miR-467, and miR-669 clusters.
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