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8 publications mentioning bmo-mir-9a

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-9a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 32
Using a dual luciferase reporter gene assay, they then predicted and verified that let-7 had target sites on the respective 3-UTRs of Ras1, Ras2, and Ras 3. To verify the regulation function of Bmo-miR-9a on the expression of Bm-ase gene, Song et al. (2013) constructed a Bmo-miR-9a over -expressing vector and Bm-ase 3'UTR fused firefly lucif-erase gene reporter plasmid, respectively. [score:7]
These results suggested that Bmo-miR-9a could down-regulate the expression of Bm-ase gene. [score:6]
By using target prediction software RNAhybrid and RNA22, Song et al. (2013) obtained a target -binding site of Bmo-miR-9a in the 3'UTR of Bm-ase gene. [score:5]
Bmo-miR-9a down regulates the expression of Bm-ase gene in vitro. [score:4]
The results confirmed that the baculovirus expression system could be used to transcribe a recombinant vector containing miR-9a for further analysis of miR-9a function (Huang et al. 2012a ). [score:3]
Construction and detection of expression vectors of microRNA-9a in BmN cells. [score:3]
Bmo-miR-9a is a conservative miRNA. [score:1]
Based on these experiments, Huang et al. 2011c successfully transformed the recombinant donor plasmid, pFastBac-miR-9a, into E. coli DH10Bac/AcNPV and transfected it into Sf21 insect cells with cational lipofec-tin. [score:1]
Huang et al. (2011c) successfully inserted the cytoplasmic actin 3 (A3) promoter and flanked sequences of the miRNA-9a (miR-9a) precursor into a pCDNA3.0 vector to construct a recombinant plasmid, which was then transfected into BmN cells. [score:1]
Furthermore, the result was similar when BmN cells were co -transfected with artificial synthetic Bmo-miR-9a mimics and Bm-ase 3'UTR fused luciferase reporter plasmid. [score:1]
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2
[+] score: 26
Using qRT-PCR approach, 6 up-regulated miRNAs (bmo-miR-275, N-11, N-45, N-58, N-46 and N-50) and 4 down-regulated miRNAs (bmo-miR-14, bmo-miR-1a, bmo-miR-9a and bmo-miR-274) were validated for the sequence data. [score:7]
In the current study, the expression of miR-9a was found to be down-regulated in BmCPV-infected 72 h midgut suggesting that miR-9a may be associated with viral infection. [score:6]
Mir-9a regulates Drosophila wing development by targeting the Drosophila LIM only protein, dLMO [51]. [score:4]
In addition, loss of miR-9a function in Drosophila peripheral nervous system leads to ectopic production of sensory organ precursors (SOPs), whereas overexpression of miR-9a results in a severe loss of SOPs [54]. [score:3]
Members of MIR-263, MIR-281 and MIR-9 were also highly expressed in the 4 samples. [score:3]
Further study revealed that miR-9a prevents apoptosis during wing development by repressing Drosophila dLMO [52]. [score:2]
Recent experiment has shown that mutant flies with reduced levels of miR-9a exhibit ectopic bristles [53]. [score:1]
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3
[+] score: 8
miR-9 family had the highest expression in moths and the lowest in larvae. [score:3]
Third, we recovered abundant miR-9 and miR-9b reads from all four libraries, and both exhibited a dynamic regulation (Figure 4). [score:2]
Overall, miR-9b level was lower than that of miR-9 in any given stage. [score:1]
First, miR-9 family represented by three members, miR-9, miR-9b and miR-9c in silkworm and these members differ by one nucleotide. [score:1]
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
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4
[+] score: 5
bmo-let-7b, bmo-let-7c, bmo-miR-9, bmo-miR-9*, bmo-miR-100-like, bmo-miR-263a, bmo-miR-31 and bmo-bantam were expressed in larva and pupa, but were not detected in moth; of these miRNAs, bmo-miR-9 and bmo-miR-9* are also complementary miRNAs. [score:3]
miRNAs having the most orthologs are mir-133 and mir-9, which are found in 25 and 23 animal species, including D. melanogaster and C. elegans. [score:1]
Weaver et al. (2007) demonstrated that some microRNAs function in the same or similar way in Drosophila and bee (e. g., mir-9a may control sensory organ precursors (SOPs) between Drosophila and bee) [52]. [score:1]
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5
[+] score: 4
These observations implicate that, orthologous miRs may regulate the orthologs of targeted genes in related species, such as, miR-9a controlling Sensory Organ Precursors (SOPs) in Drosophila and Bee [32]. [score:4]
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6
[+] score: 3
Other miRNAs from this paper: bmo-mir-275, bmo-mir-2b, bmo-mir-1926, bmo-mir-965, bmo-mir-2739
Construction and detection of expression vectors of microRNA-9a in BmN cells. [score:3]
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7
[+] score: 1
Bmo-miR-8, bmo-miR-9a, and bmo-miR-263a, similar to bmo-miR-1, showed a slight elevation after diapause-broken stage (DBS) and kept a relatively constant level thereafter. [score:1]
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8
[+] score: 1
Seven families of conserved miRNAs, including four clusters described above (2, 4, 7, and 8), have paralogs in the silkworm genome (mir-2, mir-993, mir-9, mir-92, mir-263, mir-279, and mir-989) (Figure 4). [score:1]
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