9 publications mentioning bmo-mir-8

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-8. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1

The most abundantly expressed miRNAs in B. mori are also highly conserved miRNAs e. g, miR-1, miR-8, miR-10, let-7, miR-263a, miR276a, and miR-306 and were expressed in all four stages, albeit their expression levels vary across different developmental periods.

The seed sequences of miR-8 and miR-429 are identical, indicative of a potential in targeting similar sequences (messenger RNA targets).

miR-8 is a negative regulator of Wnt signaling in Drosophila [73].

The top three abundant miRNAs of the silkworm are miR-1 (30%), miR-8 (11%) and miR-306 (11%) in the feeding larval stage; miR-1 (40%), miR-8 (18%), and miR-276a (15%) in the spinning larval stage; miR-276a (27%), miR-1 (16%) and miR278 (8%) in the pupal stage; miR-263a (18%), miR276a (16%) and miR-1 (12%) in the adult stage (Additional file 2).

However, in the silkworm, the two miRNAs vary greatly in abundance: miR-8 level was high whereas miR-429 level was extremely low.

The specific accumulation of miR* sequences for miR-8, miR-10, miR-37, miR-281 and miR-965 was particularly striking in the silkworm (Table 3 and Figure 5).

Perhaps, miR-8 plays a predominant role in Wnt signaling in B. mori.

Abundant miR-8 during the silkworm spinning-larval stage suggests a role in Wnt signaling.

Notably, miR-8 and miR-1 abundance was greater by 10-fold and 6-fold, respectively, in spinning larvae than in other stages.

2

bmo-miR-1, bmo-let-7a, bmo-miR-8, bmo-miR-14, bmo-miR-276a, bmo-miR-279 were strongly expressed in all developmental stages (larva, pupa and moth).

These newly identified miRNAs are bmo-miR-2a*, bmo-miR-8*, bmo-miR-13a*, bmo-miR-46*, bmo-miR-263*, bmo-miR-279*, and bmo-miR-305*.

Using methylated DNA probes, signal strength was significantly improved in Northern blots for bmo-miR-14 and bmo-miR-8*, which could not be detected using non-methylated DNA probes [see Figure S2 of Additional file 1].

Of these miRNAs, bmo-miR-8 has an opposite-strand miRNA, bmo-miR-8*, which was detected by (Figure 3A) at very low levels in larva, pupa and moth.

3

For instance, aside from a few commonly expressed miRNAs, such as bmo-miR-8, bmo-miR-13, bmo-miR-263a, bmo-miR-275, bmo-miR-279, bmo-282*, bmo-miR-285 and bmo-miR-306, most of them were found development-related (Table 3) although some may be due to inadequate sampling that often results less reliable statistics.

Bmo-miR-8, bmo-miR-9a, and bmo-miR-263a, similar to bmo-miR-1, showed a slight elevation after diapause-broken stage (DBS) and kept a relatively constant level thereafter.

4

Furthermore, this study revealed that the immediate-early gene targeted by bmo-miR-8 could be controlled by bmnpv-miR-1 and Ran dsRNA, resulting in increasing virus infection levels in the B. mori larvae.

The constructed miRNA vectors were successfully transfected into BmN cells and quantitative RT-PCR (qRT-PCR) analysis showed the relative abundances of bmo-mir-1a, bmo-mir-8, and bmo-mir-133 in BmN cells.

5

Bombyx mori miR-8 was identified as an anti-viral miRNA, which is suppressed by BmNPV following infection or transfection of a bmnpv-miR-1 mimic into the host cell [16].

Further analysis suggested that conserved miRNAs such as bmo-bantam, bmo-let-7, bmo-miR-31, bmo-miR-8 were in general highly abundant.

6

After a further 12 h (24 h Em), 12 miRNAs were detected soon after egg release from diapause, including the 7 "early" miRNAs and others observed for the first time (miR-252, miR-8, miR-2a, miR-79, and miR-10b-3p).

Small and large miRNA transcripts were detected in pre-laid eggs and embryos, and were identified as miR-8, miR-252 and let-7 (indicated with a red arrow in Figure 2B).

A number of miRNAs were also confirmed in females by (let-7a, let-7b, miR-8, and miR-2a) (Figure 5B).

7

Besides, Bmo-miR-8 was identified as an anti-viral miRNA, which is suppressed by BmNPV following infection or transfection of abmnpv-miR-1 into the host cell [13].

8

For instance, bmo-bantam, bmo-miR-12, bmo-miR-263a, bmo-miR-263b, bmo-miR-278, and bmo-miR-8 are both literature -based and database collections but the miRBase-collected miRNAs showed stronger signals.

9

Based on microarray analysis, 10 female-biased miRNAs were detected in the malpighian tubules (miR-286, miR-228, miR-274, miR-1, miR-252, let-7a, miR-8, bantam, miR-200b, and miR-2a), whereas miR-276-5p and miR-305 showed male-prone accumulation in malpighian tubules.