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27 publications mentioning bta-mir-30b

Open access articles that are associated with the species Bos taurus and mention the gene name mir-30b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 280
As shown in Figure 6D,E, the expression levels of Trim55(exon9+) and INSR(exon11+) in C2C12 cells transfected with miR-30-5p were obviously lower than that in control C2C12 cells, indicating that miR-30-5p could regulate the alternative splicing of the target genes of MBNL1, providing more evidence that miR-30-5p could repress MBNL1 protein expression. [score:8]
The co-transfection experiment of miR-30-5p and the non -transfected experiment showed a negative correlation between the miR-30-5p expression levels and MBNL2 and MBNL3 expression (Figure 4F,I, Figures S2 and S3), suggesting that miR-30-5p could be likely to regulate the expression of MBNL2 and MBNL3. [score:8]
miR-30a-5p, miR-30b-5p and miR-30e-5p were respectively transfected into HEK293T cells with the luciferase reporter constructs harbouring potential binding sites of miR-30-5p (MBNL1-WT) or the luciferase reporter constructs harbouring mutant potential binding sites of miR-30-5p (MBNL1-Mut); (F– H) miR-30-5p directly targets MBNL2; (I– K) miR-30-5p directly targets MBNL3. [score:7]
The expression level was normalized to U6 and relative to miR-30e-5p expression in muscle of adult cattle; (D) The differences of miR-30-5p expression in skeletal muscle among fetal, calf and adult cattle. [score:7]
Taken together, these data indicated that miR-30a-5p, miR-30b-5p and miR-30e-5p directly target the 3′ UTR of MBNL2, whereas MBNL3 is a direct target gene of miR-30a-5p and miR-30e-5p only. [score:7]
Additionally, we found that the expression of mature miR-30b-5p demonstrated a great difference in skeletal muscle during different stages of Qinchuan cattle development (Figure 2D), suggesting that miR-30b-5p might be developmentally expressed in muscle cells. [score:7]
The co-transfection experiment of miR-30-5p and the non -transfected experiment showed that there was a negative correlation between the miR-30-5p expression levels and MBNL1 (Figure 4A and Figure S1), suggesting that miR-30-5p could likely regulate MBNL1 expression. [score:6]
The prediction of TargetScan6.2 showed that the MBNL family of genes are the potential direct targets of miR-30-5p, and our data verified the prediction. [score:6]
Also, point mutation analyses showed that the miR-30-5p seed sites had no capability of targeting the mutated target sites of the 3′ UTRs (Figure 4H,K). [score:6]
Point mutation analyses showed that the miR-30-5p seed sites had no ability to target the mutated target sites of 3′ UTRs, with the exception miR-30e-5p (Figure 4E). [score:6]
The bta-miR-30a-5p, bta-miR-30b-5p and bta-miR-30e-5p targets predicted by the Targetscan6.2 algorithms [57]. [score:5]
Based on our RNAi experiments and previous study on the roles of MBNL1 and MBNL3 in muscle differentiation [25, 49], we concleded that miR-30-5p regulates muscle differentiation through directly targeting the MBNL genes. [score:5]
To study the possible function of miR-30-5p in myogenesis, we first overexpressed miR-30a-5p, miR-30b-5p and miR-30e-5p in C2C12 cells and detected the mature miR-30-5p expression level by RT-qPCR. [score:5]
We concluded that miR-30-5p directly targets MBNLs, through muscle signaling pathways, to regulate muscle differentiation. [score:5]
To obtain further information on how miR-30-5p downregulates the MBNL1 level, we determined the splice pattern of Trim55 and INSR (Figure 6A,B), which are regulated by MBNL1. [score:5]
miR-30b-5p could repress MBNL1 and MBNL2 expression, but had no effect on MBNL3 expression, which may be important to the MBNL1/MBNL3 pair that controls muscle differentiation [26]. [score:5]
Thus, the expression data of myogenic markers indicated that miR-30-5p could suppress the differentiation of C2C12 myoblasts. [score:5]
MBNL2 and MBNL3 were also predicted to be target genes for bovine miR-30-5p, according to TargetScan6.2. [score:5]
TargetScan6.2 was used to predict the target genes for bovine miR-30-5p. [score:5]
Therefore, we reasonably concluded that miR-30-5p directly targets MBNL for regulating muscle differentiation through muscle signaling pathways. [score:5]
In the cells transfected with the luciferase reporter vector containing MBNL2 3′ UTR targeting the seed sequences of miR-30a-5p, miR-30b-5p and miR-30e-5p, the luciferase activity was significantly suppressed, which was in contrast with results from the control cells (Figure 4H). [score:5]
In summary, we observed that miR-30-5p affects muscle differentiation and that MBNL1 promotes muscle differentiation, which demonstrated that the MBNL family members are the direct target genes of miR-30-5p, and confirmed that miR-30-5p could regulate alternative splicing of the INSR and Trim55 genes. [score:5]
2.3.2. miR-30-5p Directly Targets MBNL2 and MBNL3. [score:4]
2.3. miR-30-5p Directly Targets MBNL Family. [score:4]
Wei C. Li L. Gupta S. NF-κB -mediated miR-30b regulation in cardiomyocytes cell death by targeting Bcl-2 Mol. [score:4]
These results verified that MBNL1 was the target gene regulated by miR-30-5p. [score:4]
We observed that the C2C12 cells transfected with miR-30-5p had a reduced mRNA expression of IR-B, confirming that miR-30-5p regulates the alternative splicing of INSR, which suggests that miR-30-5p may control muscle differentiation through the PI3K/AKT and ERK pathway. [score:4]
Primers listed in the Table S1 for miR-30-5p and reference gene U6 were designed based on Bos taurus sequences using Beacon Designer 7.9. miRNA constructs that express miR-30-5p including miR-30a-5p, miR-30b-5p and miR-30e-5p were conducted, using the overexpression vector pcDNA3.1(+). [score:4]
The expression profiling analysis showed evidence that miR-30-5p might play a crucial role in muscle development. [score:4]
Thus, because the expression profile suggested that miR-30-5p might have an important role in muscle development, it was selected as the candidate miRNA for further research about muscle differentiation. [score:4]
2.3.1. miR-30-5p Directly Targets MBNL1. [score:4]
To obtain direct evidence that MBNL1 3′ UTRs are targets of miR-30-5p, we cloned the 3′ UTRs of the MBNL1 gene, including the miR-30-5p recognition sites, and inserted them downstream of the luciferase gene in the pGL3-control luciferase reporter vector. [score:4]
We tested the hypothesis that members of the miR-30-5p family regulate myogenesis by targeting members of the MBNL family and, thus, facilitate alternative splicing of muscle-related genes. [score:4]
As expected, the mRNA expression of Trim55 including exon9 decreased in this study, suggesting that miR-30-5p is likely to regulate more muscle-related genes like Trim55 to act on muscle differentiation. [score:4]
NF-κB -mediated miR-30b targets Bcl-2 to control cardiomyocytes cell death. [score:3]
Moreover, overlap PCR was carried out to generate the mutations in target sites of MBNL 3′ UTRs recognized by miR-30-5p, using two pairs of primers, two of them harboring the mutations (Primers listed in Table S3). [score:3]
During the previous research about high-throughput sequencing on miRNA in skeletal muscle of Chinese cattle, the miRNA family miR-30-5p (miR-30a-5p, miR-30b-5p and miR-30e-5p) was detected and was predicted to target MBNL mRNA and, thus, to play a role in myogenesis [41]. [score:3]
High expression of mature miR-30-5p was found in heart and lung tissue (Figure 2A–C). [score:3]
Figure 2Expression profile of miR-30-5p in tissues of different stage. [score:3]
According to our previous research on its expression in muscle tissue, miR-30-5p (miR-30a-5p, miR-30b-5p and miR-30e-5p) is a muscle-related miRNA [41]. [score:3]
To address the function of bovine miR-30-5p, we first detected the expression patterns in different tissues of fetal, calf, and adult Qinchuan cattle by RT-qPCR analysis. [score:3]
RT-qPCR detection of mature miR-30-5p, using RNA prepared from C2C12 cells transfected with the expression constructs of miR-30-5p during the differentiation, confirming proper processing of miR-30-5p. [score:3]
2.2. miR-30-5p Inhibits Myogenic Differentiation. [score:3]
To confirm the effect of miR-30-5p on muscle differentiation, the mixture with an equivalent amount of constructs expressing miR-30a-5p, miR-30b-5p and miR-30e-5p would be transfected into the C2C12 cells cultured in the 6-well plates. [score:3]
miR-30-5p was confirmed to repress both the mRNA and protein expression of MHC and MyoG, which provided evidence that miR-30-5p repressed muscle differentiation. [score:3]
These previous studies strongly suggested that all three members of miR-30-5p jointly participate in the auto-regulation and cross-regulation of MBNL1 and MBNL2, which means that miR-30-5p may serve an important role in the accurate modulation of MBNL1 and MBNL2 function. [score:3]
β-Tubulin was used as the loading control; (C) The C2C12 cells cultivated in DM (magnification 10×); (D) The expression levels of MHC and MyoG in C2C12 cells co -transfected with equivalent amount of miR-30a-5p, miR-30b-5p and miR-30e-5p were detected by RT-qPCR at 6 days after transfection in DM. [score:3]
The mRNA expression analysis showed that there was a lower level of MyoG and MHC in the C2C12 co -transfected with miR-30-5p than in the control C2C12 at six days of differentiation (Figure 3D). [score:3]
The Expression Profile of miR-30-5p in Different Tissues. [score:3]
As shown in Figure 3B, at six days of differentiation, the MHC and MyoG protein expression levels in C2C12 cells transfected with pcDNA3.1(+) harboring pre-miR-30-5p were significantly lower than in the control C2C12 cells. [score:3]
We individually transfected constructs expressing miR-30a-5p, miR-30b-5p and miR-30e-5p into C2C12 cells during differentiation. [score:3]
Additionally, exon5 and exon7 of MBNL1 are mainly distributed in early differentiation stages [53, 54], which suggests that miR-30-5p regulates muscle differentiation possibly by regulating alternative splicing transcripts of MBNL1 and MBNL2. [score:3]
After the transfection of miR-30a-5p, miR-30b-5p and miR-30e-5p individually into the C2C12 cells, the protein expression level of myosin heavy chain (MHC) and MyoG were examined. [score:3]
RT-qPCR detection of mature miR-30-5p, using RNA prepared from HEK293T cells transfected with the expression constructs of miR-30-5p, confirming proper processing of miR-30-5p. [score:3]
The cells transfected with pcDNA3.1(+) as the control; (C) Western blot analysis of MBNL1 protein levels regulated by miR-30-5p in the HEK293T cells respectively transfected with miR-30a-5p, miR-30b-5p and miR-30e-5p. [score:2]
MBNLs play important roles for muscle differentiation in these cells as well [25], and our present study, focusing on the role of a family of microRNAs (miR-30-5p) on alternative splicing by MBML proteins in cattle, provides novel insights into the fine-tuning of MBML activity during normal muscle tissue development (i. e., in the absence of DM). [score:2]
However, the mechanism of how miR-30-5p regulates myogenic process is not clear. [score:2]
All miR-30a-5p, miR-30b-5p and miR-30e-5p could regulate MBNL1 and MBNL2, which are highly similar proteins in the MBNL family. [score:2]
Besides this, we also found that the members of miR-30-5p showed differences in regulating the MBNL members. [score:2]
2.5. miR-30-5p Regulates the Alternative Splicing of Trim55 and INSR by MBNL1. [score:2]
Hence, we can conclude that miR-30-5p regulates muscle differentiation through MBNL1 repression. [score:2]
Mature sequences of bta-miR-30a-5p (Accession, MIMAT0003841), bta-miR-30b-5p (MIMAT0003547) and bta-miR-30e-5p (MIMAT0003799) were downloaded from miRBase and the 3′ UTR sequence of bovine MBNL1 (Accession,XM_005201835), MBNL2 (NM_001099710) and MBNL3 (XM_005227518) were downloaded from NCBI. [score:1]
The stars stand for conservatism among different sequences; (F) The similarity analysis of miR-30-5p in human, cattle and mouse. [score:1]
Western blot analysis confirmed that the endogenous MBNL1 proteins in HEK293T cells were particularly and significantly attenuated by miR-30-5p (Figure 4C). [score:1]
The sequences underlined represent the seed sequence of miR-30-5p. [score:1]
β-Tubulin was used as the loading control; (D) Sequence alignment of potential binding site of miR-30-5p in the 3′ UTR of MBNL1, MBNL2 and MBNL3. [score:1]
Aside from these findings, research about the function of miR-30 in skeletal muscles has rarely been carried out. [score:1]
In this study, we first confirmed the effect of miR-30-5p on skeletal muscle differentiation. [score:1]
The advantage of these constructs is the stability of processing mature miR-30-5p. [score:1]
The potential binding sites and the seed sequences of miR-30-5p were showed with potential binding sites underlined. [score:1]
The fragments containing the pre-miR-30-5p were then digested by HindIII and KpnI restriction enzyme (TakaRa; Tokyo, Japan) and inserted into the pcDNA3.1(+) vector with T4 DNA ligase (TakaRa; Japan), and confirmed by sequencing. [score:1]
We therefore concluded that miR-30b-5p and MBNL3 protein may have similar functions on MBNL1, suggesting that miR-30b-5p is integrated into the MBNL1/MBNL3 pair mo del to compensate for MBNL3. [score:1]
Significant difference was observed between C2C12 cells transfected with pcDNA3.1(+) containing pre-miR-30-5p fragments and cells with empty pcDNA3.1(+) (Figure 3A), indicating that the constructs successfully generated the mature miR-30-5p. [score:1]
The prediction results revealed MBNL1 to have potential sites recognized by 7~8 mer seed sequences of miR-30-5p (miR-30a-5p, miR-30b-5p and miR-30e-5p) (Figure 4D), and that the potential sites were conservative in the 3′ UTRs of mammalian MBNL1 (Figure 4D). [score:1]
Because mature miR-30-5p sequences between bovine and murine are so highly conserved (Figure 1F), we chose mouse C2C12 cells as the experimental mo del. [score:1]
Before conducting the following experiments, we had proven the ability of the miR-30-5p constructs to generate mature miRNA in HEK293T cells (Figure 4B). [score:1]
Proteins were extracted from the HEK293T transfected with the mixture of equivalent amount of miR-30-5p constructs, using RIPA buffer (Solarbio; Beijing, China) containing 1 mM PMSF (Solarbio; Beijing, China). [score:1]
Figure 3Effect of miR-30-5p on differentiation of C2C12 myoblasts. [score:1]
Several studies on miR-30 have been reported recently. [score:1]
When 90% of the area of the bottom of the 6 cm culture dish was covered, the cells were seeded in 6-well plates and grown for 24 h. Then, HEK293T cells were transfected with 2.0 μg of pcDNA3.1(+) containing pre-miR-30-5p constructs at a confluence of 90% using Lipofectamine 2000 (Invitrogen; Grand Island, NY, USA) according to the manufacturer’s protocol. [score:1]
The cultures were changed for serum-free medium prior to transfection with mixed vectors composed of 200 ng Firefly Luciferase reporter combinational constructs, 50 ng Ranilla Luciferase reporter vectors and 2.5 μg pcDNA3.1(+) containing pre-miR-30-5p constructs, using Lipofectamine 2000 (Invitrogen; Grand Island, NY, USA) according to manufacturer’s protocol. [score:1]
The mature cattle miR-30-5p (miR-30a-5p, miR-30b-5p and miR-30e-5p) from 5′ arm of of the hairpin precursors are labeled in pink; (E) Alignment of mature miR-30-5p, it showed the conserved match to the mature sequences. [score:1]
The red font stands for the mutated bases in the potential binding site; (E) Luciferase assays for the direct evidence of miR-30-5p targeing MBNL1. [score:1]
control represents the C2C12 cells not transfected by miR-30-5p. [score:1]
As a control, the empty luciferase reporter vector (control) was co -transfected into HEK293T cells with miR-30-5p. [score:1]
The cells transfected with pcDNA3.1(+) as the control; (B) Western blot detection for MHC and MyoG proteins in the differentiated C2C12 cells respectively transfected miR-30a-5p, miR-30b-5p and miR-30e-5p constructs for six days in differentiation medium (DM). [score:1]
Although bovine miR-30a-5p, miR-30b-5p and miR-30e-5p are located on the different chromosomes (Figure 1A–C), the mature miRNAs from the 5′ arm of the hairpin precursors are conserved from mouse to human species (Figure 1D–F). [score:1]
To test whether the miR-30-5p constructs could generate mature miRNA, we transfected the recombinational vectors pcDNA3.1(+) containing pre-miR-30-5p in HEK293T cells. [score:1]
DM six day control represents the C2C12 cells not transfected by miR-30-5p. [score:1]
Figure 1The analysis of miR-30-5p. [score:1]
The prediction results revealed MBNL2 and MBNL3 have potential sites recognized by 7~8 mer seed sequences of miR-30-5p (Figure 4G,J), and that the potential sites were conservative in 3′ UTRs of MBNL2 in mammal (Figure 4G), but not in MBNL3 (Figure 4J). [score:1]
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[+] score: 34
The mRNA expression of actin -binding protein PFN2, the potential target of miR-17-5p, miR-19a, miR-30b-5p, miR-142-5p, miR-301a, and miR-2478, also tended to be downregulated in the grazing cattle compared to the grain-fed cattle (P = 0.064), as did the potential miR-29b targets DNAJB2 (P = 0.021) and COL3A1 (P = 0.100). [score:9]
The potential targets of downregulated c-miRNAs were associated with protein phosphorylation and phosphate metabolism (miR-17-5p), endocytosis (miR-19a, miR-301a), mTOR signaling (miR-19a), MAPK signaling (miR-19a, miR-98), ECM-receptor interaction and adipocytokine signaling (miR-29b), skeletal muscle tissue development (miR-30b-5p), blood vessel development and morphogenesis, and regulation of actin cytoskeleton (miR-301a). [score:9]
Next, we analyzed the muscle tissue expression of the 11 miRNAs that differed significantly between the groups (miR-10b, miR-17-5p, miR-19a, miR-29b, miR-30b-5p, miR-98, miR-142-5p, miR-301a, miR-374b, miR-425-5p, and miR-652) to determine whether or not c-miRNA expression was associated with that of skeletal muscle tissue miRNAs. [score:5]
The expression of PTEN, the potential target of miR-10b, miR-17-5p, miR-19a, miR-29b, miR-30b-5p, miR-142-5p, miR-301a, miR-652, and miR-2478, was lower in the grazing cattle than in the grain-fed cattle (P = 0.011). [score:5]
In the present study, muscle PTEN and PFN2 were the predicted targets of most of the altered c-miRNAs (miR-17-5p, miR-19a, miR-30b-5p, miR-142-5p, miR-301a) between the feeding conditions, as well as miR-10b. [score:3]
The present results of microarray and qPCR revealed that the grazing JB cattle had a higher plasma content of miR-10b and lower plasma contents of miR-17-5p, miR-23b-3p, miR-28, miR-30b-5p, miR-30d, miR-98, miR-301a, miR-345-5p, miR-374b, miR-425-5p, miR-652, and miR-874 than the grain-fed cattle. [score:1]
In contrast, the grazing cattle showed significantly lower contents of miR-17-5p (P = 0.031), miR-19a (P = 0.007), miR-29b (P = 0.021), miR-30b-5p (P = 0.035), miR-98 (P = 0.006), miR-142-5p (P = 0.013), miR-301a (P = 0.005), miR-374b (P = 0.016), miR-425-5p (P = 0.010), and miR-652 (P = 0.046). [score:1]
Of those miRNAs, the contents of miR-652, miR-30d, miR-301a, miR-345-5p, miR-374b, miR-425-5p, miR-23b-3p, miR-30b-5p, miR-17-5p, miR-98, miR-28, and miR-874 were lower in the plasma of the grazing cattle than in that of the grain-fed cattle, whereas the contents of miR-10b, miR-2368-3p, miR-885, and miR-2425-3p were higher in the plasma of the grazing cattle. [score:1]
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[+] score: 34
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-181a-2, hsa-mir-205, hsa-mir-181a-1, hsa-mir-214, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-146a, hsa-mir-184, hsa-mir-186, hsa-mir-193a, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-219a-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-365a, hsa-mir-365b, hsa-mir-374a, hsa-mir-148b, hsa-mir-423, hsa-mir-486-1, hsa-mir-499a, hsa-mir-532, hsa-mir-590, bta-mir-26a-2, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-27a, bta-mir-499, bta-mir-125b-1, bta-mir-181a-2, bta-mir-205, bta-mir-27b, bta-mir-31, bta-mir-193a, bta-let-7d, bta-mir-148b, bta-mir-186, bta-mir-191, bta-mir-192, bta-mir-200a, bta-mir-214, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-532, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-365-1, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-664a, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-1915, bta-mir-146a, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-194-2, bta-mir-219-1, bta-mir-223, bta-mir-26a-1, bta-mir-365-2, bta-mir-374b, bta-mir-486, bta-mir-763, bta-mir-9-1, bta-mir-9-2, bta-mir-181a-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2339, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-664a, bta-mir-2284e, bta-mir-1388, bta-mir-194-1, bta-mir-193a-2, bta-mir-2284w, bta-mir-2284x, bta-mir-148c, hsa-mir-374c, hsa-mir-219b, hsa-mir-499b, hsa-mir-664b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, hsa-mir-486-2, hsa-mir-6516, bta-mir-2284ab, bta-mir-664b, bta-mir-6516, bta-mir-219-2, bta-mir-2284ac, bta-mir-219b, bta-mir-374c, bta-mir-148d
Within 6 hrs of the presence of E. coli, the expression of 6 miRNAs in MAC-T cells was significantly altered (P < 0.05), three were down regulated (bta-miR-193a-3p, miR-30c and miR-30b-5p) while three were up-regulated (bta-miR-365-3p, miR-184 and miR-24-3p) (Table  3). [score:7]
The three miRNAs (bta-miR-193a-3p, miR-30c and miR-30b-5p) that were significantly down regulated or one miRNA (bta-miR-365-3p) that was significantly up regulated within 6 hrs of E. coli presence only showed a retarded significant down regulation by 24 or 48 hrs (bta-miR-193a-3p, 30c and 30b-5p) or up regulation (bta-miR-365-3p) by 48 hrs in the presence of S. aureus. [score:5]
The up-regulation of miR-193a-3p and miR-30b-5p may play regulatory roles in cell death which need to be further confirmed in the context of mastitis. [score:5]
For example, gene targets of five differentially expressed miRNAs (miR-365-3p, miR-30b-5p, miR-30c, let-7a-5p and miR-23a) were enriched for pathways in immune system (B-cell receptor signaling pathway, chemokine signaling, T-cell receptor signaling and Fc gamma R -mediated phagocytosis). [score:5]
It is not surprising owing to their involvement in almost all biological processes as demonstrated by GO functional annotation of the target genes of three (bta-miR-193a-3p, miR-423-5p and miR-30b-5p) of these miRNAs. [score:3]
GO functional annotation of target genes of bta-miR-193a-3p and miR-30b-5p showed enriched genes related to cell growth and death, e. g. growth arrest specific gene 1 (GAS1), myeloid cell factor 1 (MCL1, also BCL2 related), BCL2-like 11 (apoptosis facilitator) and programmed cell death 10 (PDCD10). [score:3]
In addition, our study revealed a temporal differential regulation of five miRNAs (bta-miR-193a-3p, miR-423-5p, miR-30b-5p, miR-29c and miR-un116) in unchallenged cells. [score:2]
We observed that five miRNAs (bta-miR-193a-3p, miR-423-5p, miR-30b-5p, miR-29c and miR-un116) were differentially expressed (P < 0.05) in at least two time points in control cells as compared to 0 hr (Figure  2). [score:2]
The expression of bta-miR-30b-5p and bta-miR-29c also increased over time but to a lesser magnitude as compared to bta-miR193a-3p. [score:2]
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4
[+] score: 12
Other miRNAs from this paper: bta-mir-26a-2, bta-mir-101-2, bta-mir-148a, bta-mir-30d, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-142, bta-mir-30e, bta-mir-148b, bta-mir-186, bta-mir-191, bta-mir-22, bta-mir-30a, bta-mir-150, bta-mir-30c, bta-mir-101-1, bta-mir-141, bta-mir-146a, bta-mir-223, bta-mir-26a-1, bta-mir-30f, bta-mir-181a-1, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-1388, bta-mir-2898, bta-mir-2904-1, bta-mir-2904-2, bta-mir-2904-3, bta-mir-2284w, bta-mir-2284x, bta-mir-148c, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2284y-7, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-1842, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-148d, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Specifically, the miR-30a-5p and -30d (members of the miR-30 family) are known to be involved in regulation of autophagy in cancer progression and treatment by suppressing the expression of beclin 1 [46] and also cellular invasion and immunosuppression by targeting GalNAc transferase GALNT7 to increase synthesis of the immunosuppressive cytokine interleukin-10 [47]. [score:12]
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5
[+] score: 11
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-22, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-98, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-10a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-181a-1, hsa-mir-221, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-27b, hsa-mir-30b, hsa-mir-130a, hsa-mir-152, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-185, hsa-mir-193a, hsa-mir-320a, hsa-mir-200c, hsa-mir-1-1, hsa-mir-181b-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-99b, hsa-mir-130b, hsa-mir-30e, hsa-mir-363, hsa-mir-374a, hsa-mir-375, hsa-mir-378a, hsa-mir-148b, hsa-mir-331, hsa-mir-339, hsa-mir-423, hsa-mir-20b, hsa-mir-491, hsa-mir-193b, hsa-mir-181d, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, bta-mir-29a, bta-let-7f-2, bta-mir-148a, bta-mir-18a, bta-mir-20a, bta-mir-221, bta-mir-27a, bta-mir-30d, bta-mir-320a-2, bta-mir-99a, bta-mir-181a-2, bta-mir-27b, bta-mir-106a, bta-mir-10a, bta-mir-15b, bta-mir-181b-2, bta-mir-193a, bta-mir-20b, bta-mir-30e, bta-mir-92a-2, bta-mir-98, bta-let-7d, bta-mir-148b, bta-mir-17, bta-mir-181c, bta-mir-191, bta-mir-200c, bta-mir-22, bta-mir-29b-2, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-363, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-15a, bta-mir-19a, bta-mir-19b, bta-mir-331, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-1-2, bta-mir-1-1, bta-mir-130a, bta-mir-130b, bta-mir-152, bta-mir-181d, bta-mir-182, bta-mir-185, bta-mir-24-1, bta-mir-193b, bta-mir-29d, bta-mir-30f, bta-mir-339a, bta-mir-374b, bta-mir-375, bta-mir-378-1, bta-mir-491, bta-mir-92a-1, bta-mir-92b, bta-mir-9-1, bta-mir-9-2, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, bta-mir-320b, bta-mir-339b, bta-mir-19b-2, bta-mir-320a-1, bta-mir-193a-2, bta-mir-378-2, hsa-mir-378b, hsa-mir-320e, hsa-mir-378c, bta-mir-148c, hsa-mir-374c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-378j, bta-mir-378b, bta-mir-378c, bta-mir-378d, bta-mir-374c, bta-mir-148d
The miR-30(b/c/d/e) family regulates kidney development by targeting the transcription factor Xlim1/Lhx1 in Xenopus[66]. [score:5]
In addition, ssc-moRNA-3, belonging to new type of miRNA termed moRNA, was found at the 5’ end of pre-miR-30. [score:1]
In our study, 8 miRNA families (let-7, mir-1, mir-17, mir-181, mir-148, mir-30, mir-92 and mir-99) were found with at least 3 members among all exosome miRNAs. [score:1]
The let-7 family had 9 members, miR-181 family had 4 members (miR-181a/b/c/d) and miR-30 family had 5 members (miR-30a/b/c/d/e). [score:1]
#: due to miRNAs classification by seed sequence, 3p and 5p of miR-30 represent different miRNAs families. [score:1]
Similarly, miR-30a was the most abundant in the miR-30 family. [score:1]
At the 5’ end of pre-miR-30, a 18 nt RNA sequence was found to be generated from the loop, downstream of ssc-miR-30a-5p (Figure 10C). [score:1]
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6
[+] score: 10
14/26s highly expressed in the HM fraction (let-7d-5p, miR-103a-3p, miR-142-3p, miR-17-5p, miR-18a-5p, miR-196a-5p, miR-20a-5p, miR-24-1-5p, miR-26a-5p, miR-301a-3p, miR-30b-5p, miR-34b-5p, miR-34c-5p, miR-378a-3p) and 7/14s highly expressed in the LM fraction (miR-10b-5p, miR-122-5p, miR-1-3p, miR-184, miR-486-5p, miR-7-5p, miR-99b-5p) were predicted to target 327 and 281 experimentally observed genes, respectively. [score:7]
The relative expression in HM and LM fractions of several of these knowns, including bta-miR-103, bta-miR-30b-5p, bta-miR-17-5p, bta-miR-106b, bta-miR-142-3p, bta-miR-34b, bta-miR-18a, bta-miR-34c, bta-miR-455-5p, bta-miR-10b, bta-miR-99b, bta-miR-1246, bta-miR-99a-5p, and bta-miR-1388-5p, was consistent with the relative abundance of their homologouss, observed in the normal vs abnormal sperm. [score:3]
[1 to 20 of 2 sentences]
7
[+] score: 9
When cows were fed AL diet, expression of three ruminal miRNAs including miR-125-5p, -130a, -2376, three duodenal miRNAs including miR-2483-5p, -2286l, -2336, two hepatic miRNAs including miR-199a-3p, -2399-5p and fourteen mammary gland miRNAs such as miR-196a, -205 was positively correlated with feed (R ranged from 0.81 ~ 0.99, P < 0.05) and N efficiency (R ranged from 0.81 ~ 0.98, P < 0.05), whereas the expression ruminal miR-1296, duodenal miR-6123, two jejunal miRNAs including miR-30b-3p, -652, five hepatic miRNAs including miR-1, -2285e, -421, -455-3p, -671, and mammary gland miR-99b was negatively correlated with feed (R ranged from −0.82 ~ −0.94, P < 0.05) and N efficiency (R ranged from −0.84 ~ −0.92, P < 0.05) (Table S6). [score:5]
Our study revealed that systematic phosphorylation (duodenal miR-1296, jejunal miR-30b-3p, hepatic miR-199a-3p and mammary miR-196 (data not shown)) and transport of AAs (ruminal miR-130a, duodenal miR-1296, jejunal miR-30b-3p and mammary miR-196 (data not shown)) may be conducted by mediated-miRNAs, suggesting that miRNA -mediated mechanisms regulated N efficiency by regulating AAs transport and phosphorylation throughout all these five tissues. [score:3]
Phosphorylation of L-AAs (FDR = 5.45E-04, n = 20, such as miR-30b-3p), and transport of AAs (FDR = 7.96E-04, n = 20, such as miR-30b-3p) was predicted to be impacted by jejunum negatively associated miRNAs. [score:1]
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[+] score: 9
Other miRNAs from this paper: bta-mir-221, bta-mir-222, bta-mir-30d, bta-mir-10a, bta-mir-30e, bta-mir-10b, bta-mir-30a, bta-mir-30c, bta-mir-331, bta-mir-135a-2, bta-mir-135a-1, bta-mir-188, bta-mir-30f, bta-mir-670, bta-mir-873, bta-mir-1839, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2306, bta-mir-2308, bta-mir-2309, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2366, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2389, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2422, bta-mir-2284r, bta-mir-2284h, bta-mir-2448, bta-mir-2284o, bta-mir-2284e, bta-mir-193a-2, bta-mir-2284w, bta-mir-2284x, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-6525, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-6526-1, bta-mir-6526-2, bta-mir-2284y-7, bta-mir-6526-3, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
In porcine mammary epithelial cells, coliform mastitis induced differential expression of miR-10a/10b and miR-30b/c/f [56]. [score:3]
Challenge of bovine MAC-T mammary epithelial cells with pathogenic Staphylococcus aureus or Escherichia coli led to differential expression of seventeen miRNA genes among which were bta-miR-30b-5p and bta-miR-30c [39]. [score:3]
Thus, Streptococcus agalactiae -induced mastitis of bovine mammary glands resulted in altered expression of thirty five miRNAs including a bta-miR-30 family member, bta-miR-135a, and bta-miR-2284 family members [41]. [score:3]
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9
[+] score: 8
A total of 68 miRNAs were profiled using Firefly [®] technology, of which miRNAs miR-122 (P-value = 0.048), miR-16 (P-value = 0.042), miR-30b (P-value = 0.029), miR-320a (P-value = 0.042), miR-15b (P-value = 0.089), miR-16-2 (P-value = 0.061), miR-17 (P-value = 0.072), and miR-22 (P-value = 0.069) were found to be upregulated in female-conditioned embryo media (Figure 1). [score:4]
Both miR-30b (Ye et al., 2015) and miR-15 (Zhang et al., 2015) are involved in regulation of the epithelial-mesenchymal transition in cancer cells, which bears many similarities to the epithelial-mesenchymal transition important for successful implantation and gastrulation (Kalluri and Weinberg, 2009). [score:2]
For example, miR-30b transfected into human endometrial epithelial cells induced transcriptomic changes in the cells (Ye et al., 2015). [score:1]
miRNAs miR-122 (P-value = 0.048), miR-16 (P-value = 0.042), miR-30b (P-value = 0.029), miR-320a (P-value = 0.042), miR-15b (P-value = 0.089), miR-16-2 (P-value = 0.061), miR-17 (P-value = 0.072), and miR-22 (P-value = 0.069) were found to be significant. [score:1]
[1 to 20 of 4 sentences]
10
[+] score: 6
GO analysis also revealed potentially novel regulation, such as that of miR-27 in transcriptional and membrane/vesicle -associated processes, miR-30 in immune homeostasis and cellular metabolism and transport, and miR-33 in cardiac development. [score:3]
Although several roles have been suggested for the miR-30 family, including regulation of adipogenesis, lipid metabolism, cardiovascular disease and cancer [33], this family remains inadequately characterized. [score:2]
By analyzing AGO-bound miRNAs in MDBK cells, we found the abundance profile dominated by members of the miR-30, let-7, miR-17, miR-374, miR-21, miR-27 and miR-15 families together accounting for more than 50% (Supplementary Fig.   S2a) [14]. [score:1]
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[+] score: 5
org/), ranging from 3 target genes for miR-2892 and 1262 targets predicted for miR-30a-5p, miR-30b-5p, miR-30c, miR-30d, and miR-30f. [score:5]
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[+] score: 4
Other miRNAs from this paper: bta-let-7f-2, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-26b, bta-mir-125a, bta-mir-125b-1, bta-mir-128-1, bta-mir-181a-2, bta-mir-27b, bta-mir-140, bta-mir-15b, bta-mir-92a-2, bta-let-7d, bta-let-7g, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-125b-2, bta-mir-374a, bta-mir-128-2, bta-mir-146b, bta-mir-152, bta-mir-155, bta-mir-181d, bta-mir-24-1, bta-mir-223, bta-mir-374b, bta-mir-500, bta-mir-708, bta-mir-92a-1, bta-mir-9-1, bta-mir-9-2, bta-mir-1249, bta-mir-181a-1, bta-mir-2285a, bta-mir-2285d, bta-mir-2285b-1, bta-mir-2285c, bta-mir-2478, bta-mir-2898, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2285n-7, bta-mir-2285k-2, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2285k-5, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2285ae, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Both strains induced miR-221, ST12 induced miR-30b, miR-223, miR-374b and miR-500 but down-regulated miR-125a and miR-125b, while ST103 induced miR-222, all associated with alternative macrophage activation [34– 37]. [score:4]
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13
[+] score: 3
Two abundantly expressed miRNAs of miR-30 family, miR-30a and miR-30d, have also been considered as regulators in promoting insulin sensitivity [53]. [score:3]
[1 to 20 of 1 sentences]
14
[+] score: 3
In vivo context, studies have shown that the over -expression of miR-30b in the mouse mammary gland affects the lipid droplet formation [35]. [score:3]
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15
[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-23a, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-196a-1, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-196a-2, hsa-mir-210, hsa-mir-181a-1, hsa-mir-218-1, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-30b, hsa-mir-128-1, hsa-mir-145, hsa-mir-191, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-30c-1, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-361, hsa-mir-337, hsa-mir-148b, hsa-mir-196b, hsa-mir-425, hsa-mir-20b, hsa-mir-486-1, hsa-mir-488, hsa-mir-181d, hsa-mir-498, hsa-mir-519c, hsa-mir-520a, hsa-mir-526b, hsa-mir-520d, hsa-mir-506, hsa-mir-92b, hsa-mir-608, hsa-mir-617, hsa-mir-625, hsa-mir-641, hsa-mir-1264, hsa-mir-1271, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-30d, bta-mir-128-1, bta-mir-145, bta-mir-181a-2, bta-mir-181b-2, bta-mir-20b, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-148b, bta-mir-181c, bta-mir-191, bta-mir-210, bta-mir-23a, bta-mir-361, bta-mir-425, bta-let-7g, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-99b, hsa-mir-890, hsa-mir-888, hsa-mir-889, hsa-mir-938, hsa-mir-1184-1, hsa-mir-1203, hsa-mir-1204, hsa-mir-1265, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-128-2, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-218-1, bta-mir-296, bta-mir-30f, bta-mir-486, bta-mir-488, bta-mir-92a-1, bta-mir-92b, bta-mir-1271, bta-mir-181a-1, bta-mir-181b-1, bta-mir-148c, hsa-mir-1184-2, hsa-mir-1184-3, hsa-mir-486-2, bta-mir-1264, bta-mir-148d
Interestingly, the expression level of certain miRNA families namely, the let 7 family (let-7a, let-7c, let-7d, let-7d*, let-7e, let-7f, let-7i), miR-181 family (miR-181a, miR-181b), miR-30 family (miR-30b*, miR-30c-2*, miR-30e ), miR-425 family (miR-425, miR-425*), miR-92 family (miR-92a, miR-92a-1*, miR-92b) and miR-196 family (miR-196a, and miR-196b) were repressed in SE animal group. [score:3]
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16
[+] score: 3
When mouse pups were fed by wild-type dams or transgenic mice engineered to overexpress miR-30b by approximately an order of magnitude in milk [35], there were no significant differences in detected miR-30b levels in various organs and blood of nine mice in each group. [score:3]
[1 to 20 of 1 sentences]
17
[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26b, hsa-mir-27a, hsa-mir-31, hsa-mir-33a, hsa-mir-99a, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-147a, hsa-mir-34a, hsa-mir-182, hsa-mir-199a-2, hsa-mir-212, hsa-mir-221, hsa-mir-224, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-132, hsa-mir-142, hsa-mir-145, hsa-mir-152, hsa-mir-153-1, hsa-mir-153-2, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-127, hsa-mir-134, hsa-mir-200c, hsa-mir-106b, hsa-mir-361, hsa-mir-148b, hsa-mir-20b, hsa-mir-410, hsa-mir-202, hsa-mir-503, hsa-mir-33b, hsa-mir-643, hsa-mir-659, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-221, bta-mir-26b, bta-mir-27a, bta-mir-99a, bta-mir-125a, bta-mir-125b-1, bta-mir-145, bta-mir-199a-1, bta-mir-27b, bta-mir-31, bta-mir-127, bta-mir-142, bta-mir-20b, bta-let-7d, bta-mir-132, bta-mir-148b, bta-mir-200c, bta-mir-22, bta-mir-23a, bta-mir-29b-2, bta-mir-361, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-34a, hsa-mir-708, hsa-mir-147b, hsa-mir-877, hsa-mir-940, hsa-mir-548j, hsa-mir-302e, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-100, bta-mir-106b, bta-mir-130a, bta-mir-134, bta-mir-147, bta-mir-152, bta-mir-153-1, bta-mir-153-2, bta-mir-182, bta-mir-24-1, bta-mir-199a-2, bta-mir-202, bta-mir-212, bta-mir-224, bta-mir-33a, bta-mir-33b, bta-mir-410, bta-mir-708, bta-mir-877, bta-mir-940, bta-mir-29b-1, bta-mir-148c, bta-mir-503, bta-mir-148d
unst) TGF-beta signaling pathway miR-106b, −132, −148b-5p, −182, −212, −374a, −548j 1.75E-07 TGFBR2, TGFB2, SMAD2, SMAD3, SMAD4, BMPR2 Axon guidance miR-22-5p, −30b, −31, −33a-3p, −182, −132, −550a 2.85E-06 EFNB1, DCC, EPHB4, EPHA3, PLXNA1, PAK4 MAPK signaling pathway miR-30b, −106b, −132, −182, −212, −548j, −202-5p 6.43E-05 MAP3K1, MAP3K5, KRAS, MRAS, GRB2, FGF7 Endocytosis miR-33a-3p, −106b, −182, −374a, −374b, −202-5p 3.83E-04 RAB11FIP4, RAB11FIP2, EEA1, IGF1R, EPS15, EPN Colorectal cancer miR-30b, −33a-3p, −106b, −132, −212, −384, −494 5.94E-04 SOS1, FZD3, SMAD2, DCC, MAPK1, BAX Pathways in cancer miR-33a-3p, −107, −132, −212, −494, −495, −548j 5.95E-04 E2F1, FGF18, WNT16, FGF7, PTEN, MITF Wnt signaling pathway miR-132, −212, −33a-3p, −494, −940, −495, −548j, −107 6.73E-04 LRP5, LRP6, TCF7, PLCB4, DVL3, WNT1, WNT5A Neurotrophin signaling pathway miR-106b, −30b, −940, −182, −212, −107, 0.001267 PIK3R2, NTRK2, NTRK3, RPS6KA6, IRS1, RAC1 Oocyte meiosis miR-212, −132, −940, 495, −595, −107 0.003369 CDC27, CPEB1, PRKACB, FBXW11, MAPK1, RPS6KA3 GnRH signaling pathway miR-940, −495 0.007085 PRKCA, SRC, MAP3K2, MAPK14, GRB2, MAP3K3 Underexpressed miRNAs (Hyp vs. [score:3]
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[+] score: 3
In another study testosterone treatment was reported to alter miR-22, miR-690, miR-122, let-7a, miR-30 and let-7d expression in female rat liver [25]. [score:3]
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19
[+] score: 2
Laubier J. Castille J. Le Guillou S. Le Provost F. No effect of an elevated miR-30b level in mouse milk on its level in pup tissues RNA Biol. [score:1]
It should be mentioned that the “nutritional hypothesis” of milk-derived exosomes is primarily based on three mouse mo dels, which are inherently problematic: (1) miRNA-375 KO mice; (2) miRNA-200c/141 KO mice [82] and (3) transgenic mice presenting high levels of miRNA-30b in milk [83]. [score:1]
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[+] score: 2
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-20a, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
The miRNA families miR-181, miR-143, and miR-21 were the most abundant in control groups, while miR-21, miR-181, and miR-30 were the most abundant in animals infected with P. salmonis (Valenzuela-Miranda et al., 2017). [score:1]
Sea louse Caligus rogercresseyi, which affects Chilean aquaculture, were studied during infestation in Atlantic salmon and the most abundant families were mir-10, mir-21, mir-30, mir-181, and let7 in skin, head and kidney (Valenzuela-Muñoz et al., 2017). [score:1]
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[+] score: 2
Other miRNAs from this paper: bta-mir-30d, bta-mir-30e, bta-mir-30a, bta-mir-30c, bta-mir-30f
To enhance proper processing of short hairpin RNA (shRNA) sequences by Drosha, the myostatin siRNA sequences were modified for cloning by adding microRNA30 (mir30) sequences using methods described by (Paddison et al., 2004) (http://hannonlab. [score:1]
Myostatin shRNA mir30 amplicons were cloned into the 3′ UTR region of the enhanced green fluorescent protein (eGFP) within the PEG vector, as previously described (Golding et al., 2010). [score:1]
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22
[+] score: 2
Other miRNAs from this paper: bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-151, bta-mir-30d, bta-mir-320a-2, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-31, bta-mir-34b, bta-mir-107, bta-mir-15b, bta-mir-181b-2, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-138-2, bta-mir-181c, bta-mir-214, bta-mir-455, bta-mir-93, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-let-7a-1, bta-mir-487b, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-15a, bta-mir-34a, bta-mir-1-2, bta-mir-1-1, bta-mir-105b, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-138-1, bta-mir-152, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-206, bta-mir-30f, bta-mir-409a, bta-mir-432, bta-mir-486, bta-mir-495, bta-mir-543, bta-mir-9-1, bta-mir-9-2, bta-mir-1185, bta-mir-1271, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2384, bta-mir-2284v, bta-mir-2284q, bta-mir-2404-1, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2424, bta-mir-2284r, bta-mir-2284h, bta-mir-2404-2, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-424, bta-mir-2284w, bta-mir-2284x, bta-mir-409b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, bta-mir-133c, bta-mir-2284ab, bta-mir-2284ac
The largest miRNA family size identified was miR-2284, which consisted of 12 members, and let-7, miR-30, and miR-181/376 possessed 9, 7, and 4 members, respectively; whereas other miRNA families such as miR-1, miR-31, miR-93, and miR-206 had only one member (Additional file 1). [score:1]
This was also the case for some other miRNA families, such as bta-let-7 (from 6 to 1,434,682 reads), bta-miR-30 (from 34 to 12,681 reads) and bta-miR-181 (from 376 to 20,258 reads). [score:1]
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[+] score: 1
In five cases (bta-mir-30b, bta-mir-193a, bta-mir-345, bta-mir-365, bta-mir-423) we discovered that miRNA* are more abundant than corresponding miRNA as evidenced by higher counts of sequence reads originating from miRNA* arms of the microRNA precursor sequences (Supplemental Table S1). [score:1]
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[+] score: 1
Other miRNAs from this paper: mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-29b-1, mmu-mir-30b, mmu-mir-99a, mmu-mir-126a, mmu-mir-132, mmu-mir-141, mmu-mir-181a-2, mmu-mir-185, mmu-mir-193a, mmu-mir-199a-1, mmu-mir-200b, mmu-mir-34c, mmu-let-7d, mmu-mir-196a-1, mmu-mir-196a-2, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-20a, mmu-mir-22, mmu-mir-23a, mmu-mir-26a-1, mmu-mir-26b, mmu-mir-34a, mmu-mir-200c, mmu-mir-212, mmu-mir-181a-1, mmu-mir-26a-2, mmu-mir-29b-2, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-378a, mmu-mir-451a, mmu-mir-674, mmu-mir-423, mmu-mir-146b, bta-mir-26a-2, bta-let-7f-2, bta-mir-16b, bta-mir-20a, bta-mir-26b, bta-mir-99a, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-193a, bta-let-7d, bta-mir-132, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-22, bta-mir-23a, bta-mir-29b-2, bta-mir-423, bta-let-7g, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-23b, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-34a, bta-mir-141, bta-mir-146b, bta-mir-16a, bta-mir-185, bta-mir-196a-2, bta-mir-196a-1, bta-mir-199a-2, bta-mir-212, bta-mir-26a-1, bta-mir-29b-1, bta-mir-181a-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-2284w, bta-mir-2284x, bta-mir-2284y-1, mmu-let-7j, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285t, bta-mir-2284z-2, mmu-let-7k, mmu-mir-126b, bta-mir-2284ab, bta-mir-2284ac
Le Guillou et al. [29] recently showed that the over expression of an miRNA, miR-30b, in the mouse mammary gland results in a lactation defect characterised by the presence of acini structures with abnormally small lumen and defective of lipid droplet formation. [score:1]
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[+] score: 1
Other miRNAs from this paper: bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-21, bta-mir-27a, bta-mir-30d, bta-mir-320a-2, bta-mir-199a-1, bta-mir-27b, bta-mir-107, bta-mir-140, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-let-7g, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-103-2, bta-mir-1-2, bta-mir-1-1, bta-mir-101-1, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-143, bta-mir-152, bta-mir-154a, bta-mir-185, bta-mir-199a-2, bta-mir-206, bta-mir-30f, bta-mir-335, bta-mir-33a, bta-mir-33b, bta-mir-370, bta-mir-378-1, bta-mir-432, bta-mir-9-1, bta-mir-9-2, bta-mir-1224, bta-mir-376b, bta-mir-376d, bta-mir-376c, bta-mir-376a, bta-mir-1839, bta-mir-1185, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-199c, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-378-2, bta-mir-2284w, bta-mir-2284x, bta-mir-3432a-1, bta-mir-3432a-2, bta-mir-3604-1, bta-mir-3604-2, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-154c, bta-mir-376e, bta-mir-154b, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-6526-1, bta-mir-6526-2, bta-mir-503, bta-mir-2284y-7, bta-mir-6526-3, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-6536-1, bta-mir-2284aa-4, bta-mir-6536-2, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-378d, bta-mir-3432b, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
The largest miRNA family size identified was miR-2284, which consisted of 27 members; miR-2285, let-7, miR-30, and miR-376 possessed 22, 8, 6, and 5 members, respectively, whereas other miRNA families such as miR-107, miR-122, miR-140, and miR-1839 had only one member (Table S1). [score:1]
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[+] score: 1
A recent study reported that the fine coordination of Smo activity by the miR-30 family controlled the specification and differentiation of distinct muscle cell types of zebrafish embryos [14]. [score:1]
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[+] score: 1
In mice, miR-146a, miR-16, miR-195, miR-30, and miR-744 have been reported as reference miRNAs in blood [4]. [score:1]
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