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5 publications mentioning mmu-mir-3099

Open access articles that are associated with the species Mus musculus and mention the gene name mir-3099. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 137
The expression of miR-3099 reduced (by ~9-fold; P < 0.001) as the blastocysts developed into an early stage embryo at day 7 (E7), suggesting that miR-3099 was either expressed in a spatially restricted manner or generally down-regulated at this stage. [score:8]
Expression of miR-3099 is upregulated in differentiating neuronal/glial cellsExpression of miR-3099 was observed in the preplate of the E11.5 telencephalon and later in the cortical plate of the E13.5-E17.5 cerebral cortex, by which time the majority of the cells in these structures are committed to their respective neuronal lineages. [score:8]
Expression of miR-3099 is upregulated in differentiating neuronal/glial cells. [score:6]
Expression profiling of miR-3099 throughout embryogenesisThe expression of miR-3099 in mES cells led us to hypothesize that this miRNA may play a role in early embryogenesis and therefore we characterised its expression profile throughout development. [score:6]
Similar to the embryonic expression profiles, the diverse expression profile of miR-3099 in multiple organs of the adult mouse further supports a widespread role in the development and function of these organs. [score:6]
We analysed the expression level of miR-3099 in P19 cells (Figure 6A) and found a statistically significant (P = 0.04) ~2-fold upregulation of miR-3099 in ~50% differentiated P19 cells compared to the proliferating cells (Figure 6B). [score:5]
To specifically locate the expression of miR-3099 during embryogenesis, we performed whole mount in situ hybridisation on E9.5 embryos (n = 3) and showed that miR-3099 was expressed throughout the embryo with the exception of the developing heart (Figure 3C). [score:5]
Figure 5Expression profiling of miR-3099 novel miRNA in the whole brain of different developmental stages, different adult mouse brain regions and organs. [score:4]
The expression of miR-3099 in mES cells led us to hypothesize that this miRNA may play a role in early embryogenesis and therefore we characterised its expression profile throughout development. [score:4]
Contrasting with the almost ubiquitous expression profile in early development, miR-3099 was not detected in a few regions such as the E9.5 developing heart and the ventricular zone of the telencephalon/developing cerebrum. [score:4]
Embryo-wide expression of miR-3099 during early embryogenesis suggests a pan-regulatory role, possibly functioning as a 'housekeeping' miRNA in basic cellular processes. [score:4]
When we compared the expression of miR-3099 in various adult mouse organs to the P150 whole brain, we found significant differences in the expression levels among the organs (P < 0.001) (Figure 5C). [score:4]
Figure 6Expression of miR-3099 in. [score:3]
Figure 4Expression profiling of miR-3099 novel miRNA in E11.5-E15.5 whole embryos and the E17.5 whole brain. [score:3]
Expression of miR-3099 was observed in the preplate of the E11.5 telencephalon and later in the cortical plate of the E13.5-E17.5 cerebral cortex, by which time the majority of the cells in these structures are committed to their respective neuronal lineages. [score:3]
Using stemloop RT-qPCR, we showed that miR-3099 was expressed in E3.5 blastocysts (Figure 3B). [score:3]
Therefore, increased miR-3099 expression during P19 differentiation raises the possibility that this miRNA may have a functional role during neural differentiation or neuronal cell function. [score:3]
Section ISH of the E11.5 whole embryos showed that miR-3099 was expressed throughout the embryo, especially in the preplate of the telencephalon, somites and hindlimb region (Figure 4). [score:3]
Figure 3Expression profiling of miR-3099 novel miRNA in mouse embryonic stem cells (with conditional allele for Dicer), E3.5 blastocysts, E7 and E9.5 embryos. [score:3]
Under high magnification, miR-3099 was found to express specifically in the preplate (PP) of telencephalon (tel) (E11.5), cortical plate (CP) of the CC (E13.5-E17.5) and the germinal layer of mesencephalon (mes) (E11.5-E13.5). [score:3]
By E13.5, miR-3099 expression was restricted to the cortical plate of the cortical neuroepithelium, striatum, medial pallium (hippocampal allocortex) and subventricular/ventricular zone of the superior and inferior colliculi. [score:3]
Using the mouse whole brain, there was a significant difference (P = 0.02) in the miR-3099 expression among E11.5, E13.5, E15.5, E17.5, postnatal day (P) 1.5 and P150 samples (Figure 5A). [score:3]
Strong expression of miR-3099 was detected in the E11.5 embryo. [score:3]
In E15.5 embryos, miR-3099 expression was observed primarily in the cortical plate of the cerebral cortex. [score:3]
We also performed stemloop RT-qPCR expression analysis of miR-3099 in various regions of the mouse brain and organs. [score:3]
In E17.5 whole brains, miR-3099 expression was prominent in the cortical plate, piriform cortex and at lower levels, in the hippocampal formation. [score:3]
No significant differences (P = 0.45) in miR-3099 expression were observed among cerebellum, cerebrum, hippocampus, medulla, olfactory bulb and thalamus (Figure 5B). [score:3]
Cross sectional analysis of the telencephalon confirmed that miR-3099 was expressed in the neuroepithelium (Figure 3D). [score:3]
Cryosection of the stained embryos shows expression of miR-3099 in the neuroepithelium of the telencephalon (D, inset in C). [score:3]
A significant finding of the study was the embryo-wide expression profile of miR-3099 in mid-gestation embryos, which became restricted to the central nervous system, suggesting a role for this miRNA in neural differentiation or function. [score:3]
Expression profiling of miR-3099 throughout embryogenesis. [score:3]
The analysis revealed a novel miRNA, miR-3099, with potential regulatory effects on early embryogenesis, and involvement in neuronal cell differentiation/function in the brain during late embryonic and early neonatal development. [score:3]
MiR-3099 expression was found to be increased after E11.5 and was maintained in postnatal day 1.5 (P1.5) and P150 whole brains. [score:2]
MiR-3099 was found to be expressed at the highest level in the pancreas, followed by the thymus, large intestine, heart, small intestine, kidney, brain, testis, ovary, skin, skeletal muscle, liver, stomach and spleen. [score:2]
The stem loop RT primer contains a target site for a universal reverse primer (5'-GTAGGATGCC GCTCTCAGG-3', GeneWorks) and a target site for UniversalProbe Library (UPL) Probe #21 (Roche Diagnostics), which were used in subsequent cDNA amplification processes together with a specific forward primer for miR-3099 (5'-CGCGTAGGCT AGAGAGAGGT-3', GeneWorks). [score:2]
This finding further suggests that miR-3099 may play an important regulatory role during neurogenesis or in neuronal function. [score:2]
In situ hybridisation analysis using LNA probes for miR-scrambled and miR-3099 was performed on E11.5-E15.5 developing embryos and E17.5 whole brain paraffin sections. [score:1]
To evaluate the expression profile of miR-3099 in the later stages of embryogenesis, we performed section ISH. [score:1]
The membrane was exposed to a storage phosphor screen in a cassette at room temperature for 1 day for miR-3099 blot and 8 days for other blots before scanned using Typhoon™ 9400 (GE Healthcare). [score:1]
This novel miRNA has been identified as miR-3099. [score:1]
analysis of miR-3099 in E11.5-P150 whole brain (A), brain regions in P150 whole brain (B) (n = 2 for each group) and various mouse organs harvested from P150 adult mouse (C) (n = 2 for all except P150 whole brain, skeletal muscle, spleen, stomach and testes, where n = 3). [score:1]
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2
[+] score: 73
Targets of miR-3099: The regions of the 3′ untranslated regions (UTRs) of Vcan and Nap1l1 containing the miRNA binding sites were PCR amplified from total mouse DNA and cloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) using the following primers: Nap1l1: 5′-GATCGAGCTCGATCAGAACCCAGCCGAGT-3′ and 5′-GATCTCTAGAGCATCCCATGAGAGCTAAAACT-3′; Vcan: 5′-GATCGAGCTCGCGCTGATCCTTAAAATGGC-3′ and 5′-GATCTCTAGAATTTACATGGCCATCGGTGC3′. [score:8]
html, using default settings) predicts a set of 58 targets for unedited miR-3099, whereas 161 targets are predicted for the edited version, with only three mRNA targets present in both sets. [score:7]
In contrast, unedited miR-3099 reduced Nap1|1 reporter gene expression by 30% while the edited version had only reduced gene expression by 14%. [score:5]
As miR-3099 is edited by the essential ADAR1 enzyme (see below) we could not test for changes in the expression of predicted targets in the absence of ADAR1 in vivo. [score:5]
The genes potentially targeted by the edited form were enriched for (among others): ‘Glutamatergic synapse’ (five genes), ‘Axon guidance’ (four genes) and ‘Notch signaling pathway’ (three genes), whereas the targets of unedited miR-3099 were not (Supplementary Figure S4). [score:5]
Taken together these results demonstrate that editing in the seed region strongly redirects miR-3099 from one target to another. [score:4]
Still, to test for a change of target specificity in vivo we selected the best target predictions for unedited and edited miR-3099, Nap1l1 and Vcan, respectively and cloned their 3′UTRs into the pmiRGlo vector for analysis by dual luciferase assay. [score:4]
miR-3099 is upregulated during neuro-differentiation and might therefore also play a role in embryogenesis and neuronal function (46). [score:4]
Age- and differentiation -dependent editing of miR-3099 may well be a mechanism repressing Vcan expression in adult, differentiated tissues (45). [score:3]
Figure 3. Editing of miR-3099 changes its target specificity. [score:3]
Together this indicates that this particular editing event may lead to dramatic retargeting of miR-3099 in wild-type mice. [score:3]
Still, cotransfection of pre-edited miR-3099 led to an ∼22% reduction of the Vcan reporter while the unedited miR-3099 did not affect reporter gene expression. [score:3]
es, (47– 49)) we looked for enrichment in KEGG pathways in the two sets of predicted targets of miR-3099. [score:3]
As miR-3099 is edited up to 80% in the brain, the major amount of this miRNA has the potential to target a different set of mRNAs. [score:3]
Using a reporter assay we demonstrate that two predicted targets for edited and unedited versions of the most highly edited miRNA, miR-3099, are only affected by the edited and unedited miR-3099, respectively. [score:2]
The Vcan 3′ UTR, the predicted target of edited miR-3099, showed a 44% reduction compared to empty vector control when cotransfected with edited miR-3099 but not with the unedited version. [score:2]
In contrast, editing sites in all other newly identified edited miRNAs, except for position 2 of miR-3099, are significantly less edited in the ADAR2 mutant. [score:1]
Four of these abundant newly identified events harbor the edited adenosine in the seed region (miR-467c, position 3; miR-467e, position 4; miR-3099, position 2; miR-411, position 2). [score:1]
This would be in agreement with a possible requirement of editing of miR-3099 for its proper function in neuro-differentiation. [score:1]
The highest editing level is found in mmu-miR-3099 at position 7 of the mature sequence, which is edited to 80% in wild type, and to 66% in ADAR2 mutant brain (Table  4). [score:1]
Pri-miRNA constructs: Roughly 120-nucleotide-long sequences of mmu-pri-mir-3099 and -497 were PCR amplified from total mouse DNA and cloned into pSUPERIOR. [score:1]
miR-3099 was identified in a deep sequencing study of the developing mouse brain (46). [score:1]
We co -transfected the two different reporter constructs (pmiRGlo + Nap1l1 or Vcan 3′UTR) with either unedited or pre-edited pri-miR-3099 or empty vector control (pSuperior-neo-GFP) into human HeK293 cells and measured the effect of the two different miRNA versions on protein expression of the reporters. [score:1]
The Vcan 3′UTR responds to edited miR-3099 while the Nap1l1 UTR shows a stronger response to unedited miR-3099. [score:1]
miR-3099 has already been found to be edited at this position in other studies (18, 38, 43). [score:1]
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3
[+] score: 7
Mir466i was upregulated in NLPs, while Mir7007, mmu-mir-703, Mir107, Mir361, Mir6918, Mir6982, and Mir3099 were downregulated in NLPs. [score:7]
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4
[+] score: 2
Thus, we identified 11 putative novel testis miRNAs with high confidence and detected miR-3099-3p previously described in newborn ovary (Table 2). [score:1]
An additional 143 pre-miRNAs have been described in the latest miRBase 15.0 version but only candidate 185 has been identified as mir-3099-3p, first isolated in newborn ovaries [40]. [score:1]
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5
[+] score: 1
S7 7. Nrf2 -dependent effect of 5 mg / kg PQ Nrf2(+/+) PQ5—Nrf2(–/–) PQ5 miR-135a, miR-376c, miR-31, miR-let-7i*, miR-669b*, miR-344, miR-15b, miR-700*, miR-3099, miR-377, miR-338-5p, miR-382, miR-219-3p and miR-310a S8 8. Nrf2 -dependent effect of 10 mg / kg PQ Nrf2(+/+) PQ10—Nrf2(–/–) PQ10 miR-495*, miR-154*, miR-let-7b, miR-1983, miR-103 and miR-26a S9 The miR-380-3p / Sp3 mRNA pathway is worth to mention here. [score:1]
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