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16 publications mentioning hsa-mir-579

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-579. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 45
These results could be interpreted that either miR-579 regulates CPSF2 expression via translational repression or that mRNA changes may occur outside of the analyzed time window. [score:6]
We could show that miR-579 targets its host ZFR, and that via APA two ZFR transcripts exist, one that is targeted by its intronic miRNA, and another one that is not. [score:5]
ZFR is targeted and differentially regulated by its intronic miRNA hsa-miR-579. [score:4]
To experimentally validate the direct binding and targeting of hsa-miR-579 to its host ZFR, we subcloned its 32UTR into the MCS of the psiCheck-2 vector. [score:4]
F) In U87 cells, expression changes of the long (miRNA binding site containing; red) and short (without miRNA binding site; blue) alternatively polyadenylated UTRs after transfection with pre-miR-579 or with scrambled control was determined by quantitative RT-PCR. [score:3]
It hosts the human-specific miRNA gene hsa-mir-579 in intron 11 (intron length: 4,722 bp, distance to the upstream exon: 684 bp), which appears to be co-expressed with its host gene, as there is no bioinformatic evidence of an individual promoter region for this miRNA. [score:3]
We then chose ZFR and its intronic miRNA miR-579 as an example and could show that ZFR is in fact targeted by miR-579. [score:3]
D) In U87 cells transiently transfected with scrambled control or pre-miR-579, ZFR and CPSF2 mRNA expression was analyzed by quantitative RT-PCR. [score:3]
miR-579 targets its host, ZFR, and the APA associated gene CPSF2. [score:3]
These data show that miR-579 not only targets its host ZFR, but due to the position of the polyadenylation sites, this interaction might be differentially controlled. [score:3]
It contains a seed site for hsa-miR-579 at position-chr5:32,354,558–32,354,564 and, according to our database, APA sites at positions chr5:32,354,730, chr5:32,355,524, and chr5:32,355,823 (Fig. 2B). [score:1]
Values are shown as miR-579 transfection relative to scrambled control (n = 5; *, p < 0.05). [score:1]
B) Schematic diagram of the ZFR 32UTR including polyadenylation sites and the seed matching site for miR-579. [score:1]
Importantly, only the longest UTR isoform harbors the binding site for hsa-miR-579 at nucleotide position 1301 after the CDS. [score:1]
Since CPSF2’s 32UTR contains a seed-matching motif for miR-579 at 168 bp after the CDS, we first investigated, if CPSF2 is a target of miR-579. [score:1]
C) U87 cells were co -transfected with reporter constructs containing wildtype ZFR-32UTR or ZFR-32UTR lacking the miR-579 binding site (mut 32UTR) along with pre-miR-579 or negative control (NC). [score:1]
While CPSF2 mRNA levels were unaffected after miR-579 transfection (Fig. 2D), western blotting revealed a significant reduction in CPSF2 protein abundance (Fig. 2E). [score:1]
After pre-miR-579 transfection of U87 cells, a decrease of mRNA levels of ZFR (29%) was observed (Fig. 2D). [score:1]
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2
[+] score: 23
Main findings were: (1) low expression of miR-579-3p is a negative prognostic factor correlating with poor survival; (2) expression levels of miR-579-3p decrease during melanoma progression i. e., from nevi to stage III/IV melanoma; (3) miR-579-3p acts as oncosuppressor by targeting the 3′ untranslated region (3′UTR) of two oncoproteins (BRAF and an E3 ubiquitin protein ligase, MDM2); (4) moreover miR-579-3p ectopic expression impairs the establishment of drug resistance in human melanoma cells; and (5) miR-579-3p is strongly down-regulated in matched tumor samples from patients before and after the development of resistance to targeted therapies [37] and was also identified in cell lines resistant to BRAF/MEK inhibitors. [score:21]
miR-579-3p is a novel master regulator of melanoma progression and drug resistance metastatic melanoma. [score:2]
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3
[+] score: 15
Six of these miRNAs miR-16, miR-302d-3p, miR-378e, miR-570–3p, miR-574-5p, miR-579; were down-regulated and one was up-regulated miR-25-3p in T1DM plasma-derived exosome samples compared to the control (p value < 0.05) (Figs  2B and 3). [score:6]
We identified and confirmed that of the seven miRNAs whose levels change in plasma exosomes from T1DM subjects, 6 which were lower in T1DM patients (miR-16, miR-302d-3p, miR-378e, miR-570-3p, miR-574-5p, and miR-579) and 1 upregulated (miR-25-3p) several appear to play important roles in diabetes. [score:4]
miRNA miRBase ID sequence hsa-miR-16-5p MIMAT0000069 uagcagcacguaaauauuggcg hsa-miR-25-3p MIMAT0000081 cauugcacuugucucggucuga hsa-miR-302d-3p MIMAT0000718 uaagugcuuccauguuugagugu hsa-miR-378e MIMAT0018927 acuggacuuggagucagga hsa-miR-570-3p MIMAT0003235 cgaaaacagcaauuaccuuugc hsa -miRNA- 574-5p MIMAT0004795 ugagugugugugugugagugugu hsa -miRNA-579 MIMAT0003244 uucauuugguauaaaccgcgauu hsa -miRNA- 631 MIMAT0003300 agaccuggcccagaccucagc hsa -miRNA-let-7 MIMAT0000062 ugagguaguagguuguauaguu hsa-RNU6-2 — acgcaaattcgtgaagcgtt Because of the absence of a validated reference genes in the plasma exosome samples for the normalization of exosome microRNA expression data, it was critical to choose an appropriate housekeeping microRNA. [score:3]
To validate the RNA sequencing data, we performed a qRT-PCR analysis of hsa-let-7, hsa-miR-631, RNU6, hsa-miR-16-5p, hsa-miR-25-3p, hsa-miR-302d-3p, hsa-miR-378e, hsa-miR-570-3p, hsa-miR-574-5p, and hsa-miR-579. [score:1]
Furthermore, miR-16, miR-570, miR-574 and miR-579 have been found in extracellular vesicles released from human pancreatic islets [18]. [score:1]
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4
[+] score: 14
Besides has-miR-579, the other four nodes were up-regulated differentially expressed miRNAs. [score:6]
Hsa-miR-579 was a down-regulated miRNA of ovarian cancer samples, and miR-579 is found to dysregulated in colorectal cancer with liver metastasis [21]. [score:5]
According to their degrees in MFSN, hsa-miR-579, hsa-miR-942, hsa-miR-105, hsa-miR-150, and hsa-miR-27a* were selected as hub nodes in MFSN. [score:1]
In all, 5 nodes with degrees more than 30, including hsa-miR-579 (36), hsa-miR-942 (31), hsa-miR-105 (31), hsa-miR-150 (34), and hsa-miR-27a* (32) were selected as the hub nodes in MFSN. [score:1]
GO: 0007268 (synaptic transmission) and GO: 0019226 (transmission of nerve impulse) were the two common functions of miRNAs in MFSN, and hsa-miR-579 (36), hsa-miR-942 (31), hsa-miR-105 (31), hsa-miR-150 (34), and hsa-miR-27a* (32) were selected as the hub nodes in MFSN. [score:1]
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5
[+] score: 14
Further, mol-mir168a showed sequence homology with hsa-miR579, a human miRNA that normally regulates TNFα expression during endotoxin tolerance [38]. [score:4]
Based on these results we identified mol-miR168a homologous to hsa-miR579, a human miRNA with many target genes including SIRT1. [score:3]
We focused our attention on mol-miR168a because this is the first plant miRNA involved in cross-kingdom activity (osa-miR168a shown high sequence homology with mol-miR168a) [14] and it has functional homology with hsa-miR579, a putative regulator of the SIRT1 gene. [score:2]
Human miRNA name M. oleifera miRNA name r-value Sequence alignment details hsa-miR6503-3p mol-miR166i 0.67 -GGAC-AGG-U-CA--CC-CC hsa-miR548ah-5p mol-miR393c 0.65 -AAAG-GAU-GCA-UG-U-- hsa-miR3940-5p mol-miR159a 0.65 -UGG-UUG--G-G-GCUCU- hsa-miR579 mol-miR168a 0.57 UC--UUGGU--A---CG-GA- hsa-miR4534 mol-miR159a 0.71 GGAU-GA-G-G-G-UCU hsa-miR1306-3p mol-miR6478 0.78 AC-UU-GCUC-G-UGGUG hsa-miR4703-3p mol-miR6300 0.72 GU-GUUGUA-U-UA-UG- hsa-miR5008-5p mol-miR6300 0.67 G-C-UUG--G-A-AGUGG hsa-miR4273 mol-miR398c 0.67 GUGUUCUC-G-U-G-C--COMIR software [33] predicted human genes that could be regulated by M. oleifera miRNAs. [score:2]
Human miRNA name M. oleifera miRNA name r-value Sequence alignment details hsa-miR6503-3p mol-miR166i 0.67 -GGAC-AGG-U-CA--CC-CC hsa-miR548ah-5p mol-miR393c 0.65 -AAAG-GAU-GCA-UG-U-- hsa-miR3940-5p mol-miR159a 0.65 -UGG-UUG--G-G-GCUCU- hsa-miR579 mol-miR168a 0.57 UC--UUGGU--A---CG-GA- hsa-miR4534 mol-miR159a 0.71 GGAU-GA-G-G-G-UCU hsa-miR1306-3p mol-miR6478 0.78 AC-UU-GCUC-G-UGGUG hsa-miR4703-3p mol-miR6300 0.72 GU-GUUGUA-U-UA-UG- hsa-miR5008-5p mol-miR6300 0.67 G-C-UUG--G-A-AGUGG hsa-miR4273 mol-miR398c 0.67 GUGUUCUC-G-U-G-C-- COMIR software [33] predicted human genes that could be regulated by M. oleifera miRNAs. [score:2]
The transfection of synthetic mol-miR168a, a plant miRNA that show sequences homology with hsa-miR579, determined a significant decrease of SIRT1 protein level in comparison with HF and control samples (Fig 9B and 9C). [score:1]
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6
[+] score: 13
MiR-335-5p and miR-579-3p were upregulated in both groups, whereas miR-126-5p was upregulated in clinical samples and downregulated in the mo del system. [score:10]
MiR-335-5p, miR-579-3p, and miR-126-5p were shared by the expression profiles of HPV -positive tonsillar tumors and of the HPV immortalized keratinocyte clones, whereas miR-328-3p, miR-34c-3p, and miR-885-5p were shared by the miRNA profiles of HPV -negative tonsillar tumors and the HPV -negative keratinocytes. [score:3]
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7
[+] score: 7
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-26b, hsa-mir-29a, hsa-mir-30a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-15b, mmu-mir-29b-1, mmu-mir-30a, mmu-mir-30b, mmu-mir-125a, mmu-mir-125b-2, mmu-mir-130a, mmu-mir-138-2, mmu-mir-181a-2, mmu-mir-182, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-10a, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-181a-1, mmu-mir-297a-1, mmu-mir-297a-2, mmu-mir-301a, mmu-mir-34c, mmu-mir-34b, mmu-let-7d, mmu-mir-106a, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-138-2, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-138-1, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-26b, mmu-mir-29a, mmu-mir-29c, mmu-mir-34a, rno-mir-301a, rno-let-7d, rno-mir-344a-1, mmu-mir-344-1, rno-mir-346, mmu-mir-346, rno-mir-352, hsa-mir-181b-2, mmu-mir-10a, mmu-mir-181a-1, mmu-mir-29b-2, mmu-mir-138-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-125b-1, hsa-mir-106b, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-34b, hsa-mir-34c, hsa-mir-301a, hsa-mir-30e, hsa-mir-362, mmu-mir-362, hsa-mir-369, hsa-mir-374a, mmu-mir-181b-2, hsa-mir-346, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-10a, rno-mir-15b, rno-mir-26b, rno-mir-29b-2, rno-mir-29a, rno-mir-29b-1, rno-mir-29c-1, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-34b, rno-mir-34c, rno-mir-34a, rno-mir-106b, rno-mir-125a, rno-mir-125b-1, rno-mir-125b-2, rno-mir-130a, rno-mir-138-2, rno-mir-138-1, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-181a-1, hsa-mir-449a, mmu-mir-449a, rno-mir-449a, mmu-mir-463, mmu-mir-466a, hsa-mir-483, hsa-mir-493, hsa-mir-181d, hsa-mir-499a, hsa-mir-504, mmu-mir-483, rno-mir-483, mmu-mir-369, rno-mir-493, rno-mir-369, rno-mir-374, hsa-mir-582, hsa-mir-615, hsa-mir-652, hsa-mir-449b, rno-mir-499, hsa-mir-767, hsa-mir-449c, hsa-mir-762, mmu-mir-301b, mmu-mir-374b, mmu-mir-762, mmu-mir-344d-3, mmu-mir-344d-1, mmu-mir-673, mmu-mir-344d-2, mmu-mir-449c, mmu-mir-692-1, mmu-mir-692-2, mmu-mir-669b, mmu-mir-499, mmu-mir-652, mmu-mir-615, mmu-mir-804, mmu-mir-181d, mmu-mir-879, mmu-mir-297a-3, mmu-mir-297a-4, mmu-mir-344-2, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-493, mmu-mir-504, mmu-mir-466d, mmu-mir-449b, hsa-mir-374b, hsa-mir-301b, rno-mir-466b-1, rno-mir-466b-2, rno-mir-466c, rno-mir-879, mmu-mir-582, rno-mir-181d, rno-mir-182, rno-mir-301b, rno-mir-463, rno-mir-673, rno-mir-652, mmu-mir-466l, mmu-mir-669k, mmu-mir-466i, mmu-mir-669i, mmu-mir-669h, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-466j, mmu-mir-1193, mmu-mir-767, rno-mir-362, rno-mir-504, rno-mir-582, rno-mir-615, mmu-mir-3080, mmu-mir-466m, mmu-mir-466o, mmu-mir-466c-2, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466p, mmu-mir-466n, mmu-mir-344e, mmu-mir-344b, mmu-mir-344c, mmu-mir-344g, mmu-mir-344f, mmu-mir-374c, mmu-mir-466b-8, hsa-mir-466, hsa-mir-1193, rno-mir-449c, rno-mir-344b-2, rno-mir-466d, rno-mir-344a-2, rno-mir-1193, rno-mir-344b-1, hsa-mir-374c, hsa-mir-499b, mmu-mir-466q, mmu-mir-344h-1, mmu-mir-344h-2, mmu-mir-344i, rno-mir-344i, rno-mir-344g, mmu-let-7j, mmu-mir-30f, mmu-let-7k, mmu-mir-692-3, rno-let-7g, rno-mir-15a, rno-mir-762, mmu-mir-466c-3, rno-mir-29c-2, rno-mir-29b-3, rno-mir-344b-3, rno-mir-466b-3, rno-mir-466b-4
Of these miRNAs, 12 were upregulated (miR-34b, miR-138, miR-297a, miR-301, miR-449, miR-466, miR-493, miR-579, miR-582, miR. [score:4]
1Proliferation, Invasion, Tumor suppression [63– 66] miR-344 ↓2.0 ↓3.2 NA miR-346 ↓2.4Proliferation [67, 68] miR-362 ↓2.3Proliferation, Invasion, Apoptosis [69– 76] miR-369 ↓2.8 ↓2.6 ↓2.1Aerobic glycolysis [77] miR-374 ↑3.0 ↓2.2 NA miR-449 ↑2.7 ↑2.4Proliferation [78– 81] miR-463 ↓2.7 NAmiR-466 [°] ↑2.4 ↑2.1 ↓3.5 NA miR-483 ↓3.2Apoptosis [82] miR-493 ↑2.1 ↓2.2Proliferation [83– 85] miR-499a ↓5.0 ↑2.3Proliferation [86] miR-504 ↓2.6 ↑2.0Proliferation, Apoptosis [87, 88] miR-579 ↑2.8 NAmiR-582 [^] ↑2.4Proliferation [89] miR-615 ↓2.1Proliferation, Invasion [90, 91] miR-652 ↑2.4Proliferation, EMT [92, 93] miR-669b ↓2.1 NA miR-669h ↓3.6 ↑2.3 NA miR-669i ↓2.3 NA miR-669k ↓7.2 ↓5. [score:3]
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8
[+] score: 6
Other miRNAs which are known to target TNF-α expression in LPS-tolerised cells are miR-221, miR-579 and miR-125b [30]. [score:5]
However, mimic transfection of miR-221, miR-579 and miR-125b only restores TNF-α levels to approximately 80% that of non-tolerised cells [30]. [score:1]
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9
[+] score: 6
As mentioned in Table  4, the miRNA hsa-miR-579 targets a total of seventeen genes from different gene-modules out of which two genes (i. e., SPRY2 and YBX2) belong to blue module, another two genes (i. e., two specific GNAS family proteins) are coming from brown module, one gene (viz. [score:3]
On the other hand, as mentioned in [38], the topmost miRNA namely hsa-miR-579 has a connection with Alzheimer’s Disease; whereas the second top miRNA entitled as hsa-miR-495 relates to HIV mediated dementia [39]. [score:3]
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10
[+] score: 5
Eight miRNAs (let-7d, and miR-579, -107, -195, -152, -424, -130b, and -143) were shared by the three MPRNs and simultaneously downregulated under all the three manipulations. [score:4]
All of these eight miRNAs but miR-579 and -424 have been reported to be associated with glioma. [score:1]
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11
[+] score: 4
Lastly, 7 hubs from the 11 hubs discovered by DIANA-microT are retrieved within the 40 degree sorted nodes on the TargetScan network (in decreasing order by degree: miR-548c-3p, miR-590-3p, miR-579, miR-186, miR-513a-3p, miR-661, miR-495 and lastly miR-940). [score:3]
It comprises miR-495, miR-548c-3p, miR-590-3p, miR-186, miR-579, miR-513a-3p, miR-543 and miR-944. [score:1]
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12
[+] score: 4
The top 20 miRNAs significantly up- or down-regulated in Exo [Hypoxic] compared to Exo [Normoxic] are listed in Table 1. Nine miRNAs were expressed at a significantly lower level in Exo [Hypoxic] compared to Exo [Normoxic]: miR-521 (Fold change = 0.0005), miR-27a (Fold change = 0.24), miR-324 (Fold change = 0.446), miR-579 (Fold change = 0.448), miR-502 (Fold change = 0.396), miR-222 (Fold change = 0.232), miR-135b (Fold change = 0.325), miR-146a (Fold change = 0.456) and miR-491(Fold change = 0.482). [score:4]
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13
[+] score: 3
miR-579-3p controls melanoma progression and resistance to target therapy. [score:3]
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14
[+] score: 3
However, neither miR-125b nor other microRNAs known to target TNFα (miR-221 and miR-579) [53] were detected in our screen. [score:3]
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15
[+] score: 3
Ten miRNAs (let-7e, miR-128, miR-323-3p, miR-133b, miR-18b, miR-144, miR-451, miR-150, miR-486-3p, and miR-196b-5p) reflected a fourfold differential expression, and three miRNAs (miR-130b-5p, miR-452, and miR-579) were only identified in plasma from RA patients. [score:3]
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16
[+] score: 3
For example, rearrangements of chromosome 5, especially 5p gain, are often related to cervical cancer [45], and a miRNA located at 5p13.3, miR-579, is predicted to target the genes PTGS2 and IRF1, which are involved in cervical cancer. [score:3]
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