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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-20a, hsa-mir-24-2, hsa-mir-93, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-125a, hsa-mir-196a-1, hsa-mir-10a, hsa-mir-181c, hsa-mir-196a-2, hsa-mir-218-1, hsa-mir-218-2, hsa-mir-222, mmu-let-7d, mmu-mir-106a, hsa-let-7g, hsa-let-7i, hsa-mir-125a, mmu-mir-196a-1, mmu-mir-196a-2, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-20a, mmu-mir-24-2, mmu-mir-93, mmu-mir-338, mmu-mir-340, mmu-mir-10a, mmu-mir-17, mmu-mir-218-1, mmu-mir-218-2, mmu-mir-222, mmu-mir-181c, hsa-mir-375, mmu-mir-375, hsa-mir-340, hsa-mir-338, hsa-mir-335, mmu-mir-335, hsa-mir-196b, mmu-mir-196b, mmu-mir-301b, hsa-mir-301b, mmu-mir-1896, mmu-let-7j, mmu-let-7k
We noticed that there were many miRNAs, such as mir-340-3p, mir-340-5p, mir-1896, mir-546, and mir-301b, whose expression were not significantly affected by Activin A or Wnt3a alone at progression or maturation stages, but regulated by Activin A and Wnt3a co-treatment.
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Other miRNAs from this paper: hsa-let-7e, hsa-mir-29a, hsa-mir-32, mmu-mir-126a, mmu-mir-142a, mmu-mir-146a, mmu-mir-194-1, mmu-mir-195a, hsa-mir-139, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-212, hsa-mir-223, mmu-mir-290a, hsa-mir-142, hsa-mir-126, hsa-mir-146a, hsa-mir-194-1, hsa-mir-195, mmu-let-7e, mmu-mir-29a, rno-mir-7a-1, mmu-mir-32, mmu-mir-139, mmu-mir-212, mmu-mir-223, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-362, mmu-mir-362, rno-let-7e, rno-mir-7a-2, rno-mir-7b, rno-mir-29a, rno-mir-32, rno-mir-126a, rno-mir-139, rno-mir-142, rno-mir-146a, rno-mir-194-1, rno-mir-194-2, rno-mir-195, rno-mir-212, rno-mir-223, hsa-mir-451a, mmu-mir-451a, rno-mir-451, hsa-mir-483, hsa-mir-146b, hsa-mir-497, mmu-mir-483, rno-mir-483, rno-mir-497, mmu-mir-497a, mmu-mir-146b, mmu-mir-710, rno-mir-146b, rno-mir-362, rno-mir-126b, hsa-mir-451b, mmu-mir-195b, mmu-mir-451b, mmu-mir-126b, mmu-mir-142b, mmu-mir-497b
B) The level of let-7e, miR-212-3p, miR-546 and miR-710 in exosomes from MIN6B1 treated or not with cytokines for 48 h cells was determined by qPCR.
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For example, miR-546 and miR-710 were increased in response to cytokines whereas let-7e and miR-212-3p were more abundant in exosomes of untreated MIN6B1 cells (see Additional file 3: Figure S2B for confirmation of these results by qPCR).
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Other miRNAs from this paper: mmu-mir-1a-1, mmu-mir-30a, mmu-mir-30b, mmu-mir-101a, mmu-mir-133a-1, mmu-mir-140, mmu-mir-145a, mmu-mir-151, mmu-mir-181a-2, mmu-mir-184, mmu-mir-195a, mmu-mir-30e, mmu-mir-301a, mmu-let-7d, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-mir-208a, mmu-mir-15a, mmu-mir-21a, mmu-mir-22, mmu-mir-322, mmu-mir-148b, mmu-mir-1a-2, mmu-mir-100, mmu-mir-210, mmu-mir-181a-1, mmu-mir-222, mmu-mir-133a-2, mmu-mir-370, mmu-mir-448, mmu-mir-451a, mmu-mir-463, mmu-mir-541, mmu-mir-449c, mmu-mir-499, mmu-mir-711, mmu-mir-721, mmu-mir-743b, mmu-mir-881, mmu-mir-883a, mmu-mir-883b, mmu-mir-147, mmu-mir-873a, mmu-mir-208b, mmu-mir-1b, mmu-mir-1195, mmu-mir-1927, mmu-mir-1933, mmu-mir-1943, mmu-mir-669o, mmu-mir-3067, mmu-mir-3074-1, mmu-mir-3102, mmu-mir-3074-2, mmu-mir-3470a, mmu-mir-3470b, mmu-mir-5123, mmu-mir-195b, mmu-mir-145b, mmu-mir-21b, mmu-mir-21c, mmu-mir-873b, mmu-mir-451b, mmu-mir-30f
86 miR-873-as 1,016 e-miR-743b-5p 23.75 miR-449c-as 515e-miR-715 || 69.35 miR-541-as 439 e-miR-881* 56.21 miR-148b-as 336 e-miR-370 97.41 miR-546-as 333 e-miR-3067 100 miR-3074-as 262 e-miR-448-5p 100miR-451-as ‡ 286 e-miR-669o-5p 99.35 † Novel miR* that are processed within the expected window of the mature strand are labelled “generic”.
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The intragenic miRNAs, miR-26a-2 (miR-26a-2 and miR-546) cluster and miR-26b, are both associated with CpG islands of 1752 and 1098 bp near the transcription start site (TSS) in the host gene or overlapping with the predicted host gene promoter, respectively.
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